急性颅脑损伤后血清内源性类洋地黄物质含量变化

目的 探讨颅脑损伤后血清内源性类洋地黄物质（ＥＤＬＳ）含量变化及其机理和临床意义。方法 采用放免法检测６３例急性颅脑外伤患者血清ＥＤＬＳ浓度变化,对其中１９便作动态观测,１６例行手术前后对比,２３例与预后进行相关分析。结果 不同程度脑外伤皇２４小时内血清ＥＬＤＳ均升高,以轻伤组最为典型;随着损伤程度的加剧,少数病例ＥＤＬＳ不变甚至下降,术后或随伤情的恢复,ＥＤＬＳ多同步下降,不同病病理类型的脑损伤     

The Contribution of Cdc2 in Rotenone-Induced G2/M Arrest and Caspase-3-Dependent Apoptosis

Neuronal cell cycle reentry maintained in a G2-like state before cell death, has been confirmed in dopaminergic neurons of patients with Parkinson's disease (PD). Caspase-3 is a final effector in apoptotic dopaminergic neurons in patients. The association of aberrant G2/M regulation with caspase-3 dependent apoptosis remains to be elucidated. Cell division cycle protein 2 (Cdc2) is a key player in G2/M transition in mitotic cells. Although the deregulation of Cdc2 correlated with the control of apoptosis in neurons, the molecular pathway by which Cdc2 involves in apoptosis is not clear. In a rotenone-based cell model of PD, we demonstrated that rotenone arrested cell cycle at G2/M phase and activated caspase-3 both in cytoplasm and nucleus. The decreased activity of Cdc2 by roscovitine or rotenone enhanced G2/M arrest. The increased cells in G2/M arrest by rotenone upregulated the expression of Cdc2. Suppression of Cdc2 expression downregulated cleaved caspase-3/9 and delayed cell apoptosis. Used together, the upregulation of Cdc2 contributes to rotenone-induced caspase-3/9-dependent apoptosis, which is associated with the enhancement of G2/M arrest. Our results suggest the deregulation of Cdc2 as a transition between cell cycle arrest and cell death.     

大肠癌组织中VEGF、MIF的表达及临床意义

目的：探讨大肠癌组织中血管内皮生长因子（VEGF）及人巨噬细胞游走抑制因子（MIF）的表达及临床意义。方法选取80份经手术切除并经病理证实为大肠癌的组织蜡块（观察组）及80份距癌肿边缘5cm以上经病理证实无癌细胞残存的正常大肠组织（对照组）,SP法检测VEGF、MIF在大肠癌及正常大肠组织中的表达。结果VEGF,MIF在观察组中阳性率为77．50％、82．50％,在对照组阳性率为22．50％、27．50％,观察组阳性率明显高于对照组,差异有统计学意义（P＜0．05）。VEGF、MIF在大肠癌组织中的表达与肿瘤的浸润深度、淋巴结转移、临床分期相关,与性别、年龄、组织学分化无关。结论VEGF、MIF在大肠癌组织中高表达。     

冠心病人心房收缩时间间期测定及其意义

以心阻抗图法对52例冠心病人进行心房收缩时间间期(ASTI)的测量,并与100例同龄组正常人对比,结果表明:冠心病人心房射血前时间缩短(P<0.01),心房射血时间延长(P<0.01),二者比值下降(P<0.01)。ASTI 对于无创性评定冠心病人心室舒张期顺应性改变是一种简单有效的新方法。     

必止咳胶囊制剂工艺研究

采用正交设计,以苦杏仁中指标成分苦杏仁甙的含量为指标,对必止咳胶囊的制剂工艺条件进行试验考察,优选出最佳生产工艺路线及提取工艺条件.     

含奥沙利铂方案介入治疗中晚期肝癌的疗效观察

目的观察含奥沙利铂方案介入治疗中晚期肝癌的疗效和不良反应.方法对79例中晚期肝癌患者在肝动脉中灌注奥沙利铂、表阿霉素、5-氟脲嘧啶,每例完成2～3次后评价疗效及不良反应.结果 79例中完全有效(CR)3例(3.8%),部分有效(PR)54例(68.4%),稳定(SD)16例(20.2%),进展(PD)6例(7.6%),总有效率(CR PR)为72.2%(57/79).甲胎蛋白下降幅度为73.3%(33/45).主要不良反应为胃肠道反应及骨髓抑制.结论含奥沙利铂方案介入治疗中晚期肝癌具有较高的疗效,不良反应发生率低.     

房室间隔缺损的二维超声心动图临床及X线分析

本文报告运用二维超声心动图技术,结合临床和X线检查,诊断房室间隔缺损54例,手术证实15例。结果表明:二维超声心动图对诊断房室间隔缺损具有重要价值。在进行这项检查时,如结合做超声造影、临床和X线表现,对较小的房室间隔缺损也能得到明确诊断。     

Giant cervical goiter in Hashimoto’s thyroiditis: A case report

A giant cervical goiter, defined as a thyroid mass larger than 8 cm in diameter, is usually a nodular or adenomatous goiter. A giant cervical goiter can also be caused by hyperthyroidism (i.e., Hashimoto’s thyroiditis). The surgical indications for patients with Hashimoto’s disease include suspected malignant tumors, persistent symptoms related to the disease, or persistent enlargement of the goiter. We herein describe a woman who developed symptoms of compression from a thyroid tumor, the volume of which was almost the largest reported in the relevant literature to date. The bilateral lobes of the giant thyroid tumor were removed by total en bloc excision. We protected the bilateral recurrent laryngeal nerve and preserved the bilateral upper and lower parathyroid glands in situ. The excised left lobe tumor was 16 × 9 × 5.5 cm, whereas the right lobe tumor was 12 × 8 × 4 cm. The pathological diagnosis was Hashimoto’s thyroiditis. Although surgical excision is difficult, it is still the main treatment modality for giant goiters in patients with Hashimoto’s thyroiditis and can help to reduce the occurrence of complications.     

联合应用取栓术及动脉溶栓治疗158例急性下肢动脉栓塞

[背景]探讨球囊导管取栓术及动脉置管溶栓联合应用治疗下肢动脉栓塞的效果.[病例报告]利用球囊导管给158例急性下肢动脉栓塞患者进行下肢动脉取栓术,术后在动脉内留置导管,持续滴入溶栓药物,连续2～4d.治疗成功者为128例(81.0%),好转为20例(12.7%),有效率为93.7%(148/158);截肢者为7例(4.2%);所有患者术后踝臂指数提高为0.75～0.90,平均为0.83;未见穿刺部位明显血肿及球囊扩张导致夹层形成等并发症发生.[讨论]下肢动脉取栓术及置管溶栓联合应用对下肢动脉栓塞治疗有明显的效果.     

胆石症胆道感染并发心包积脓7例分析

本院26年中胆石症、胆道感染并发心包积脓7例。其中6例死亡,作者指出,凡胆石症并感染的患者出现心肺症状和体征,应警惕本并发症的发生。A型超声和X线检查对诊断有重要帮助。一旦确诊应及时行心包穿刺抽脓或心包造口引流;及时有效地处理胆道原发病灶是预防和减少本并发症的根本措施。