全文获取类型
收费全文 | 5971篇 |
免费 | 276篇 |
国内免费 | 61篇 |
专业分类
耳鼻咽喉 | 53篇 |
儿科学 | 93篇 |
妇产科学 | 18篇 |
基础医学 | 761篇 |
口腔科学 | 155篇 |
临床医学 | 320篇 |
内科学 | 1592篇 |
皮肤病学 | 154篇 |
神经病学 | 281篇 |
特种医学 | 245篇 |
外科学 | 1010篇 |
综合类 | 30篇 |
预防医学 | 231篇 |
眼科学 | 83篇 |
药学 | 411篇 |
中国医学 | 36篇 |
肿瘤学 | 835篇 |
出版年
2023年 | 37篇 |
2022年 | 74篇 |
2021年 | 135篇 |
2020年 | 74篇 |
2019年 | 103篇 |
2018年 | 107篇 |
2017年 | 122篇 |
2016年 | 150篇 |
2015年 | 146篇 |
2014年 | 179篇 |
2013年 | 242篇 |
2012年 | 387篇 |
2011年 | 402篇 |
2010年 | 231篇 |
2009年 | 216篇 |
2008年 | 366篇 |
2007年 | 414篇 |
2006年 | 421篇 |
2005年 | 443篇 |
2004年 | 417篇 |
2003年 | 410篇 |
2002年 | 391篇 |
2001年 | 59篇 |
2000年 | 49篇 |
1999年 | 85篇 |
1998年 | 94篇 |
1997年 | 73篇 |
1996年 | 87篇 |
1995年 | 53篇 |
1994年 | 38篇 |
1993年 | 41篇 |
1992年 | 28篇 |
1991年 | 21篇 |
1990年 | 22篇 |
1989年 | 35篇 |
1988年 | 23篇 |
1987年 | 13篇 |
1986年 | 13篇 |
1985年 | 13篇 |
1984年 | 18篇 |
1983年 | 8篇 |
1982年 | 11篇 |
1981年 | 9篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1976年 | 6篇 |
1975年 | 7篇 |
1968年 | 3篇 |
排序方式: 共有6308条查询结果,搜索用时 0 毫秒
61.
Expression of apoptosis-related proteins in adenomyotic uteri treated with danazol and GnRH agonists. 总被引:2,自引:0,他引:2
Ken Ueki Koji Kumagai Hidetoshi Yamashita Zhong-lian Li Minoru Ueki Yoshinori Otsuki 《International journal of gynecological pathology》2004,23(3):248-258
The biologic properties of adenomyosis and the effects of therapeutic agents on adenomyosis were evaluated with immunohistochemistry, terminal deoxy-nucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method, transmission electron microscopy, and analysis of genomic abnormality. In the adenomyotic endometrium, estrogen receptor (ER) expression was more intense than in the eutopic endometrium during the secretory phase, and bcl-2 was constantly expressed throughout the menstrual cycle. The expression of ER and bcl-2 was weaker in the adenomyotic endometrium treated with danazol than in that treated with gonadotro-pin-releasing hormone agonist (GnRHa), whereas bcl-2 phosphorylated on serine-87 was more intensely expressed in danazol-treated adenomyotic endometrium than in the GnRHa-treated one. The number of TUNEL-positive cells increased in the adenomyotic endometrium treated with danazol or GnRHa. Ultrastructurally, most of the adenomyotic endometrial cells treated with danazol underwent postapoptotic necrosis and formed a cluster of dead cells. In contrast, cells treated with GnRHa underwent typical apoptosis and were sparsely distributed in the adenomyotic endometrium. Analysis of several cancer-related genes showed no microsatellite instability or loss of heterozygosity in adenomyotic tissues. Therefore, we conclude that the occurrence of adenomyosis is correlated to bcl-2 expression regulated by estrogen and ER rather than genetic mutation. 相似文献
62.
Nishiguchi S Shiomi S Sasaki N Iwata Y Tanaka H Kubo S Hirohashi K Ochi H 《Annals of nuclear medicine》2000,14(5):383-386
A 39-year-old woman with acute cholecystitis and gallstones underwent laparoscopic cholecystectomy. She suffered from recurrent episodes of cholangitis due to injury of the major bile ducts during laparoscopic cholecystectomy. Hepatobiliary scintigraphy with Tc-99m Sn-N-pyridoxyl-5-methyltryptophan was performed. Although normal bile excretion was found from the left hepatic duct to the percutaneous transhepatic biliary drainage (PTBD) tube, excretion from the right hepatic lobe was prolonged. Scintigraphy with Tc-99m diethylenetriaminepentaacetic acid-galactosyl human serum albumin demonstrated atrophy of the right hepatic lobe and enlargement of the left hepatic lobe. Cholangiography via the PTBD tube revealed complete obstruction of the left hepatico-jejunal anastomosis and could not enhance the right intrahepatic bile duct. A right hepatic lobectomy was performed because of the atrophy, glissonitis and the absence of an appropriate bile duct for reconstruction. Postoperatively she was active and exhibited no evidence of recurrent cholangitis. 相似文献
63.
Keyaki Sasaki Tomoaki Morioka Natsumi Okada Yuka Natsuki Yoshinori Kakutani Akinobu Ochi Yuko Yamazaki Tetsuo Shoji Takashi Ohmura Masanori Emoto 《Journal of diabetes investigation.》2022,13(7):1286
Fulminant type 1 diabetes is characterized by a rapid progression of insulin deficiency triggered by viral infection. Here, we report a case of a 45‐year‐old Japanese woman with fulminant type 1 diabetes that developed 8 days after receiving messenger ribonucleic acid vaccine against severe acute respiratory syndrome coronavirus 2. She had been healthy and had no symptoms suggestive of viral infection before the vaccination. Laboratory tests showed exhaustion of insulin secretion and negative results for islet autoantibodies. Human leukocyte antigen genotype analysis showed the DRB1*04:05 and DQB1*04:01 alleles. This is the first case report of new‐onset fulminant type 1 diabetes after severe acute respiratory syndrome coronavirus 2 vaccination, and suggests that a severe acute respiratory syndrome coronavirus 2 vaccine might trigger the onset of fulminant type 1 diabetes in susceptible individuals. However, a causal relationship remains to be identified, and further studies are required to determine the incidence of such cases. 相似文献
64.
Seven africanes (1, 2a,b, 3-6), two of them new (1, 2a), three secoafricanes (7-9), one of them new (7), and two norsecoafricanes (10, 11a), one of them new (10), all of them swartzianin-type, have been isolated from an Argentine collection of the endemic liverwort Porella swartziana. The structures of the new compounds were established by extensive 1D and 2D NMR spectroscopic data. Absolute configurations of compounds 2a, 2b, and 10 were derived on the basis of CD spectra. The compounds were tested for activity against a variety of microbes, but none were found to exhibit significant antibacterial activity. 相似文献
65.
66.
Tetsuro Uchida Yoshinori Kuroda Atsushi Yamashita Mitsuaki Sadahiro 《Journal of cardiac surgery》2019,34(12):1673-1675
Currently, total arch replacement (TAR) using frozen elephant trunk (FET) technique is gaining popularity for various thoracic aortic pathologies. FROZENIX is the first commercialized open stent‐graft (OSG) in Japan, and it is a useful alternative to previously self‐made OSG. FROZENIX is easy to deploy compared with formerly launched OSGs. However, some challenges have been identified with FROZENIX. Herein, we present the cases of two patients in whom we encountered serious unexpected kinking of FROZENIX during TAR performed using FET, which was related to its structural property. The clinical course and the bailout are described. 相似文献
67.
Benjamin D. Varco-Merth William Brantley Alejandra Marenco Derick D. Duell Devin N. Fachko Brian Richardson Kathleen Busman-Sahay Danica Shao Walter Flores Kathleen Engelman Yoshinori Fukazawa Scott W. Wong Rebecca L. Skalsky Jeremy Smedley Michael K. Axthelm Jeffrey D. Lifson Jacob D. Estes Paul T. Edlefsen Louis J. Picker Cheryl M.A. Cameron Timothy J. Henrich Afam A. Okoye 《The Journal of clinical investigation》2022,132(10)
Proliferation of latently infected CD4+ T cells with replication-competent proviruses is an important mechanism contributing to HIV persistence during antiretroviral therapy (ART). One approach to targeting this latent cell expansion is to inhibit mTOR, a regulatory kinase involved with cell growth, metabolism, and proliferation. Here, we determined the effects of chronic mTOR inhibition with rapamycin with or without T cell activation in SIV-infected rhesus macaques (RMs) on ART. Rapamycin perturbed the expression of multiple genes and signaling pathways important for cellular proliferation and substantially decreased the frequency of proliferating CD4+ memory T cells (TM cells) in blood and tissues. However, levels of cell-associated SIV DNA and SIV RNA were not markedly different between rapamycin-treated RMs and controls during ART. T cell activation with an anti-CD3LALA antibody induced increases in SIV RNA in plasma of RMs on rapamycin, consistent with SIV production. However, upon ART cessation, both rapamycin and CD3LALA–treated and control-treated RMs rebounded in less than 12 days, with no difference in the time to viral rebound or post-ART viral load set points. These results indicate that, while rapamycin can decrease the proliferation of CD4+ TM cells, chronic mTOR inhibition alone or in combination with T cell activation was not sufficient to disrupt the stability of the SIV reservoir. 相似文献
68.
Yoshida H Mamada Y Taniai N Mizuguchi Y Kakinuma D Ishikawa Y Kanda T Matsumoto S Bando K Akimaru K Tajiri T 《Journal of Hepato-Biliary-Pancreatic Surgery》2008,15(2):178-182
Background/Purpose According to the General rules for the clinical and pathological study of primary liver cancer, compiled by the Liver Cancer Study Group of Japan, ruptured hepatocellular carcinoma (HCC) is classified as stage 4, even
if the tumor is small and solitary. We examined the long-term results of elective hepatectomy for the treatment of ruptured
HCC.
Methods A first hepatectomy was performed without operative death in 193 patients with HCC. Ten patients had ruptured HCC (ruptured
group) and 183 patients had nonruptured HCC (nonruptured group). The extension of HCC was macroscopically classified as stage
1 in 23 patients, stage 2 in 71, stage 3 in 53, and stage 4 in 46.
Results Cumulative survival rates in the ruptured group at 1, 5, and 10 years were 90.0%, 67.5%, and 20.3%, respectively. The cumulative
survival rate was lower in patients with stage 4 disease in the nonruptured group than that in patients in the ruptured group
(P < 0.05). Cumulative survival rates did not differ significantly between patients in the ruptured group and those with stage
2 or stage 3 disease.
Conclusions Survival rates after elective hepatectomy in patients with ruptured HCC are good, even if the disease is classified as stage
4. 相似文献
69.
70.
Yoshinori Matsuoka Yukishi Nakayama Tomoko Yamada Akira Nakagawachi Kouichi Matsumoto Kimihide Nakamura Kyousuke Sugiyama Yoshinori Tanigawa Yoshinobu Kakiuchi Yoshiro Sakaguchi 《The Brazilian journal of infectious diseases》2013,17(1):7-12
Background and aimsVibrio vulnificus causes an infectious disease that has extremely poor convalescence and leads to necrotic fasciitis. In this study, we sought to define the characteristic epidemiology of V. vulnificus infection and clarify its diagnosis at the global level.MethodsOver a period of 10 years, we investigated the appearance of symptoms, underlying conditions, treatment, and mortality in 12 patients (eight men, four women; >50 years old; average age, 66 years,) infected with V. vulnificus.ResultsThe development of symptoms occurred primarily between June and September, a period during which seawater temperature rises and the prevalence of V. vulnificus increases. All patients had underlying diseases, and seven patients reported a history of consuming fresh fish and uncooked shellfish. The patients developed sepsis and fever with sharp pain in the limbs. Limb abnormalities were observed on visual examination. All patients underwent debridement; however, in the survival group, the involved limb was amputated early in 80% patients. The mortality rate was 58.3%.ConclusionRecognition of the characteristic epidemiology and clinical features of this disease is important, and positive debridement should be performed on suspicion. When the illness reaches an advanced stage, however, amputation should be the immediate treatment of choice. 相似文献