瘦体素透过表皮生长因子接受器转活化於鼠主动脉平滑肌细胞诱发内皮素表现

INTRODUCTION Obesity is a major risk factor for the development of diabetes. OBESITY AND DIABETES SPECIFIC AIMS The aim of the present study was to clarify the regulation of endothelin-1 by leptin in vascular smooth muscle cells. We further determined the intracellular signal transduction pathways focusing especially on epidermal growth factor receptor transactivation involved in this process.     

血管紧张素Ⅱ在血管中经由氧化张力引起的讯息传递

高血压、动脉粥状硬化、高血脂症以及糖尿病都会以起血管功能性及结构性的异常。这些异常包括内皮功能失常、改变血管收缩力以及血管重组。在这些变化过程中常会牵涉到血管平滑肌的增生、细胞雕零、细胞迁移、发炎以及纤维化。在许多影响因子中，血管紧张素（angiotensionⅡ，AngⅡ）无疑是属最重要的一个。血管紧张素会诱发内皮（endothelin-1）的产生，而内皮素在血管紧张素的作用中，扮演重要的调节角色。此外，血管紧张素会诱使氧游离基（reactive oxygen species．ROS）上升。在本报告中我们将焦点集中在血管紧张素、内皮素以及ROS在血管平滑肌增生过程中所扮演的角色。     

Dietary Calcium Decreases Plasma Cholesterol Level only in Female but not in Male Hamster Fed a High Cholesterol Diet

ObjectiveTo investigate the effect of dietary calcium on plasma lipoprotein profile in castrated and ovariectomized hamsters.MethodsMale, castrated, female and ovariectomized hamsters (n=36 each group) were randomly divided into three sub-groups (n=12) and fed one of the three diets containing 0, 2, and 8 g calcium per kg diet for a period of six weeks. Changes in plasma lipoprotein profile were monitored at the end of week 0, 3 and 6.ResultsPlasma total cholesterol (TC), non-high density lipoprotein cholesterol (non-HDL-C), triacylglycerols (TG) and TC/HDL-C were decreased only in intact female and ovariectomized hamsters. In contrast, three levels of dietary calcium had no effect on lipoprotein profiles in both intact male and castrated hamsters.ConclusionBeneficial modification of lipoprotein profile by dietary calcium was gender-dependent at least in hamsters.     

Clinical Significance of Induced Atrial Tachycardia After Termination of Longstanding Persistent Atrial Fibrillation Using a Stepwise Approach

Induced Atrial Tachyarrhythmia During Atrial Fibrillation Ablation. Introduction: The utility of inducibility test of atrial tachycardia (AT) in patients with longstanding persistent atrial fibrillation (AF; LPAF) is unclear. This study aimed to evaluate the significance of induced AT and the impact of their ablation on the clinical outcome. Methods: In 194 patients with LPAF (>1 year) who underwent catheter ablation (pulmonary vein isolation with substrate ablation), an inducibility test was performed after AF termination. Results: AT was induced in 108 (56%) patients (induced AT group); neither AT nor AF was inducible in 37 (19%, noninduction group). During 39 ± 21 months, AT recurred in 30 patients (28%), AF in 19 (17%), and no arrhythmia in 56 (52%) among induced AT group, although there was a recurrence of AT in 9 (24%, P = 0.68), AF in 6 (16%, P = 0.85), and no arrhythmia in 22 (60%, P = 0.42) among noninduction group (P = NS). Note that 10 patients with repeated ablation in induced AT group revealed 8 different and 2 similar recurrent ATs compared to the induced ATs at first session. The mean cycle length of induced AT that terminated by ablation (271 ± 64 ms) was longer than that without (249 ± 58 ms, P < 0.05). In induced AT group, AT recurrence rate in patients who achieved AT termination by ablation was lower than those without termination (5% vs 36%, P < 0.05). Conclusions: ATs that are inducible after LPAF termination do not necessarily become clinical AT. However, patients who achieved noninducibility of AT by ablating slower cycle length of AT had better outcomes. (J Cardiovasc Electrophysiol, Vol. 23, pp. 1171–1178, November 2012)     

LSCM观察三七总皂甙及其组分对游离钙离子影像变化的影响

用盖玻片或ＭａｔＴｅｋ培养皿培养猪主动脉内皮细胞（ａＥＣ）约１８ｈ后，以荧光探针（ｆｌｕｏ－３／ＡＭ终浓度１０μｍｏｌ·Ｌ－１）负载，当细胞内游离钙离子（Ｃａ２＋）与ｆｌｕｏ－３结合后，通过激光扫描共聚焦显微镜（ＩｎＳＩＧＨＴ－ｐｌｕｓＩＱ型）观察细胞的荧光影像变化。基于三七总皂甙（ｔ－ＰＮＳ）对ａＥＣ所产生的生物活性物质有明显的作用，本拟进一步探索上述药物对ａＥＣ中［Ｃａ２＋］；的影响。实验结果显示：在ＡＴＰ作用后，ｔ－ＰＮＳ可使ａＥＣ内的荧光强度在２０～８０ｓ内急剧升高至峰值。但其组分Ｒｂ１及Ｒｇ１使荧光强度有下降或无明显变化。提示ｔ－ＰＮＳ有使内皮细胞［Ｃａ２＋］；升高的作用。     

JUVENILE CHRONIC ARTHRITIS AND WEGENER'S GRANULOMATO SIS

We present a case of Wegener's granulomatosis in a teenage boywith systemic onset juvenile chronic arthritis. We discuss theimplication of these two uncommon conditions coexisting in onepatient. The recent characterization of the antineu trophilcytoplasmic antibody (ANCA) as a marker of small vessel vasculitishas helped in the differential diagnosis of pul monary renalsyndromes. KEY WORDS: ANCA, 1gM ANCA, Systemic vasculitis.     

Serine and d-serine position-1 substituted angiotensin II analogues Synthesis using new 2,3,5,6-tetrafluorophenyl active esters and biological activities

[Ser¹], ( 32 ), [d -Ser¹]- ( 29 ), [Ser¹, Leu⁸]- ( 31 ), and [d -Ser¹, Leu⁸] angiotensin II ( 30 ) were synthesized by a repetitive method in solution using new protected amino acid 2,3,5,6-tetrafluorophenyl active esters. 32 and 29 were agonists, and 31 and 30 were specific antagonists to angiotensin II (AII) receptors determined by the rabbit aortic strip (RAS) and rat blood pressure (RBP) assays. It was found that the hydroxymethyl side chains of serine and D-serine in position-l has an important influence on the agonistic activity of the analogues. The pressor activities of 32 and 29 were 129 and 314%, respectively, as potent as AII. On the other hand, 31 and 30 were effective in antagonizing the AII-induced contraction of RAS and rise in RBP.