糖尿病患者胰岛素基因启动子的突变检测和临床应用

目的 :探讨胰岛素基因启动子突变是否与中国人 2型糖尿病相关。方法 :随机选择黑龙江省中国汉族人 89例 2型糖尿病 ,应用聚合酶链式反应———单链象多态性 (PCR -SS CP)检测方法检测胰岛素启动子突变。结果 :89例 2型糖尿病中未发现 1例异常泳动变化。结论 :胰岛素基因启动子突变可能不是中国人 2型糖尿病的重要遗传因素。文献报道美国黑人中胰岛素基因启动子突变 ( 8个碱基TGGTCTAA的重复序列 )与 2型糖尿病相关 ,本研究结果提示此种相关有明显的种族异质性     

Single-cell transcriptome atlas reveals protective characteristics of COVID-19 mRNA vaccine

Messenger RNA (mRNA) vaccines are promising alternatives to conventional vaccines in many aspects. We previously developed a lipopolyplex (LPP)-based mRNA vaccine (SW0123) that demonstrated robust immunogenicity and strong protective capacity against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection in mice and rhesus macaques. However, the immune profiles and mechanisms of pulmonary protection induced by SW0123 remain unclear. Through high-resolution single-cell analysis, we found that SW0123 vaccination effectively suppressed SARS-CoV-2-induced inflammatory responses by inhibiting the recruitment of proinflammatory macrophages and increasing the frequency of polymorphonuclear myeloid-derived suppressor cells. In addition, the apoptotic process in both lung epithelial and endothelial cells was significantly inhibited, which was proposed to be one major mechanism contributing to vaccine-induced lung protection. Cell−cell interaction in the lung compartment was also altered by vaccination. These data collectively unravel the mechanisms by which the SW0123 protects against lung damage caused by SARS-CoV-2 infection.     

列车乘务员SCL—90评定结果分析

采用ＳＣＬ—９０症状自评量表对某铁路分局４６７名列车乘务员的心理卫生状况进行了分析。结果显示总均分、阳性症状均分、阳性项目数、九组症候群因子分、达到或超过中等严重程度的发生频数均显著高于对照组。经多元逐步回归分析,仅细菌总数和二氧化碳对列车乘务员心理卫生状况有一定影响,其原因多与工作环境的不良因素和工作性质有关。     

糖尿病中医证型的分布及动态资料对比分析

目的:掌握糖尿病住院患者中医证型的分布及动态发展资料。方法:运用统计学方法,对本院1996-1998年所有糖尿病患者的中医病证进行编码、分类、统计。结果:1996年糖尿病患者中医证候分布主要顺位为:肺胃燥热证(29.8%)、气阴两虚证(25.7%)、肾阴亏虚证(22.2%)、阴阳两虚证类(11.1%)。1997年中医证候谱发生变化,顺位为气阴两虚证(41.4%)、肺胃燥热证(24.5%)、阴阳两虚证(20.7%)、肾阴亏虚证(13.8%)。1998年为肺胃燥热证(33.2%)、气阴两虚证(30.5%)、阴阳两虚证(21.3%)、肾阴亏虚证(14.9%)。结论:危害糖尿病患者较重的阴阳两虚证所占病例逐年增多,要引起高度警惕,如不及时治疗,消渴病证候将向严重方向转移,威胁患者生命     

新生血管性青光眼视乳头筛板结构的研究

采用正常死者眼球和新生血管性青光眼的筛板组织, 研究高眼压对视乳头筛板和结缔组织的作用． 正常眼球１２ 只, 新生血管性青光眼眼球１２ 只, 摘除眼球后用１０ ％ 福尔马林溶液固定, 切取视乳头组织约２ ｍ ｍ ×２ ｍｍ , 石蜡包埋, 苏木素－ 伊红染色, 免疫细胞化学 Ａ Ｂ Ｃ 法染色． 结果： 正常人筛板的横纵切面具有特定形态, 新生血管性青光眼筛板孔板变形, 筛孔周围结缔组织断裂, 丧失了筛板结构． 结果表明：高眼压下筛孔板和结缔组织的改变, 造成对筛板孔神经纤维的挤压, 阻滞了神经纤维轴浆的运输, 导致神经纤维功能上的损害和相应的视野缺损．     

新生儿胆红素脑病23例临床分析

１９９５年１月－１９９７年１２月仅三年收治新生儿胆红素脑病２３例,有增长趋势,引起胆红素脑病的病因依次是溶血（６５．２％）低体重（５６．５％）,感染（４７．８％）特别提出必须重视对体重〈２．５ｋｇ,低于胎龄儿生后一周内的管理,医学教育上应把新生儿黄疸,胆红素脑病作为必,必考的课程,对其层从事内儿科,妇产科的医务人员也要进行有关的继续教育。     

针刺关元穴对小鼠红细胞免疫功能影响的实验研究

目的：探讨针刺关元穴对机体红细胞免疫的影响。方法：将小鼠随机分为对照组和针刺组,针刺组每日针刺关元穴,对照组不予针刺。１１ 日后采用补体致敏酵母菌血凝法,并分别测定各组小鼠的脾脏系数,检测针刺关元穴对小鼠红细胞免疫粘附功能的影响。结果：在血凝滴度为１：１６ 时,对照组与针刺组小鼠的阳性率有显著性差异（Ｐ＜ ０．０１）;脾脏系数分别为：对照组（５．７９±０．８８ｍ ｇ／ｇ）,针刺组（６．０８±０．３４）ｍ ｇ／ｇ。针刺组明显高于对照组（Ｐ＜ ０．０５）。结论：针刺关元穴对小鼠的红细胞免疫粘附功能有增强作用。     

鼻咽癌中与已知基因同源的差异表达cDNA序列

采用新近发展的ｃＤＮＡ代表性差异分析法筛选鼻咽癌中不表达的或表达降低的ｃＤＮＡ序列。结果显示：有９个与已知基因高度同源的ｃＤＮＡ序列。通过对这些已知基因的结构和功能分析,发现有与细胞骨架成分相关的基因：αａｃｔｉｎｉｎ,ｅｚｒｉｎ和细胞角蛋白１３;直接与瘤基因和抑瘤基因相互作用的基因：鲨烯合成酶和ＴＲＩＰ１基因;直接参与ＤＮＡ合成以及调控基因转录和翻译的基因：ＴＡＦⅡ６８和组蛋白Ｈ１０;另外还有人类补体因子Ｂ及类转运ＲＮＡ合成酶的基因。这些基因大多具有相当于抑瘤基因的功能。从而进一步说明鼻咽癌的发生是多基因相互作用的结果。     

Effects of topical epinephrine on aqueous humor dynamics in the cat

The purpose of this study was to investigate, in cats, the effects of topical epinephrine on aqueous humor dynamics as measured by the non-invasive method of fluorophotometry and by other methods. Measurements were carried out on 12 cats before and after one week of twice daily treatment with 2% epinephrine hydrochloride to one eye. Aqueous flow and outflow facility were determined using fluorophotometry. Uveoscleral outflow was calculated from these results and was evaluated with anterior chamber perfusion of FITC-dextran. Outflow facility also was measured by tonography. Epinephrine-treated eyes, compared with their baseline values, showed a 31% reduction in intraocular pressure (P<0.001), a 23% reduction in aqueous flow (P<0.05), a 60% increase in fluorophotometric outflow facility (P<0.05), and a 43% increase in tonographic outflow facility (P<0.05). Treated eyes, compared with contralateral control eyes, showed a 27% reduction in IOP (P<0.005), a 25% reduction in aqueous flow (P<0.005), a 38% increase in fluorophotometric outflow facility (P<0.05), and a 34% increase in tonographic outflow facility. When evaluated by both fluorophotometry and FITC-dextran tracer methods, epinephrine had no significant effect on uveoscleral outflow. It was concluded that, in cats treated with topical epinephrine twice daily for a week, a reduction in intraocular pressure is induced by an increase in outflow facility and decrease in aqueous flow.     

准分子激光原位角膜磨削术后继发圆锥角膜病因分析

目的 对４眼准分子激光原位角膜磨削术（ＬＡＳＩＫ）后继发圆锥角膜进行病因分析。方法 为４眼重度近视患者行常规ＬＡＳＩＫ治疗,术后角膜地形图分析确诊圆锥角膜,对手术前皇检查的各项数值行回顾性分析。结果 ４眼ＬＡＳＩＫ术后患者激光切削后剩余角膜基质厚度均大于２００ｕｍ,眼压正常,术后７个月～１年半发生圆锥角膜,其中１眼行穿透性角膜移植术获得满意疗效。结论 虽然ＬＡＳＩＫ术后保留角膜常规厚度,眼压正常,