桡动脉穿刺冠状动脉内介入治疗的临床应用

目的：探讨经桡动脉穿刺选择性冠状动脉造影及冠状动脉腔内成形术（PTCA）及支架术的临床意义。方法：对102例具备冠状动脉造影适应证的患者,采用经桡动脉穿刺法行右、左冠状动脉造影,并根据冠状动脉造影结果决定是否行经皮冠状动脉腔内成形术（PTCA）及支架术。结果：冠状动脉狭窄（50-70）％21例（21／102,占21％）,〉75％以上68例（68／102,占67％）,68例行PTCA及支架植入术,〈50％13例（13／102,占12％）,冠状动脉正常25例（25／70,占35．5％）。结论：通过冠状动脉造影,可以定性诊断,指导治疗;桡动脉穿刺具有出血并发症少等优点。     

分子靶向治疗:肿瘤治疗的里程碑

在上两个世纪里,肿瘤治疗出现过两次飞跃,一次是Halsted提出肿瘤根治术,另一次是Fish将化学治疗整合于根治术。此后,肿瘤治疗徘徊不前,直到分子靶向治疗出现。分子靶向治疗指使用小分子化合物、单克隆抗体、多肽等物质特异性干预调节肿瘤细胞生物学行为的信号通路,从而抑制肿瘤发展。临床实践证明,分子靶向治疗不仅能"杀灭肿瘤",而且能诱导肿瘤细胞向正常细胞分化而"治愈肿瘤",或通过抑制癌基因信号、延缓肿瘤肿瘤发展而使患者"带瘤生存",在将来有可能将恶性肿瘤转化成类似糖尿病、高血压的慢性病。本文概述了分子靶向治疗的理论基础、标志性成果和发展趋势。     

DNA-PKcs在鼻咽癌组织中的表达及其临床意义

背景与目的:DNA双链断裂(DNA double-strand break,DSB)是射线杀灭肿瘤细胞的主要机制,而同源重组(homologous recombination,HR)和非同源性末端连接(DNA nonhomologous end-joining,NHEJ)是DSB的两条重要修复途径.DNA-PK催化亚单位(catalytic subunit of DNA-dependent protein kinase,DNAPKcs)是NHEJ途径的主要修复蛋白之一,在DSB中发挥着重要作用.本研究旨在通过检测鼻咽癌组织中DNA-PKcs的表达情况,探讨其与鼻咽癌临床病理特征及预后的关系.方法:采用免疫组化SP法检测223例鼻咽癌患者组织中DNA.PKcs的表达,并结合患者的临床及预后资料进行分析.结果:223例鼻咽癌患者中,DNA.PKcs的过表达率为36.8%.卡方检验显示,DNA.PKcs表达强弱与患者的性别、年龄、病理分型及N分期的相关性均无统计学意义(P>0.05),与TNM分期、T分期、M分期均有关(P<0.05).单因素生存分析显示,DNA-PKes过表达患者5年总生存率低于低表达患者(54.6%vs.79.4%),差异有统计学意义(P<0.05).多因素Cox回归模型分析显示T、N、M分期及DNA-PKcs表达水平是影响鼻咽癌患者预后的独立因素(P<0.05).结论:DNA-PKcs在大部分鼻咽癌组织中均有表达,DNA-PKcs的表达水平与鼻咽癌患者预后相关,对患者的预后判断有一定的指导意义.     

健脾温胃祛瘀汤治疗慢性浅表性胃炎42例疗效观察

目的:观察健脾温胃祛瘀汤治疗慢性浅表性胃炎的临床疗效。方法:将84例本病患者随机分为治疗组和对照组各42例,治疗组采用健脾温胃祛瘀汤治疗,对照组采用硫糖铝和猴头菌片治疗。结果:治疗组与对照组的总有效率分别为92.86%和73.81%,两组比较,P<0.05。结论:健脾温胃祛瘀汤治疗慢性浅表性胃炎疗效满意。     

区域军队药材供应机构分布优化模型的构建与应用

目的:优化区域内军队药材供应机构与被保障对象单位的保障关系,实现药材供应机构资源的合理利用与配置.方法:采用专家咨询、运筹学整数规划等方法,按照"就近、就便保障"的联勤保障原则建立药材供应机构分布优化模型.结果:构建了基于主渠道供应及药材供应机构位置不变的药材供应机构分布优化模型,并对某区域药材供应保障关系优化进行了应用.结论:初步运用提示分布优化模型合理,进一步结合地理信息技术与决策支持系统,可为区域药材供应机构保障关系调整提供信息化工具.     

健胃舒分煎颗粒治疗功能性消化不良30例总结

目的:观察健胃舒分煎颗粒治疗功能性消化不良(FD)的临床疗效。方法:将90例FD患者随机分为3组,健胃舒组口服健胃舒分煎颗粒,每日1剂;胃苏组口服胃苏颗粒,每日15g;莫沙比利组口服莫沙比利,每日15mg;疗程均为2个月;于治疗前后进行临床证候疗效评价、胃电图(EGG)测定、胃动素和胃泌素检测。结果:3组患者临床证候均明显改善;健胃舒组总有效率为96.67%,胃苏组总有效率为83.34%,莫沙比利组总有效率为76.67%;3组治疗后胃电节律、胃电幅度均较治疗前明显改善,而健胃舒组明显优于胃苏组和莫沙比利组(P<0.05或P<0.01);3组治疗后胃泌素和胃动素分泌均较治疗前有显著性差异(P<0.01),其中胃动素分泌治疗前后差值健胃舒组明显优于胃苏组和莫沙比利组(P<0.05);且有效病例1年后复发率健胃舒组为27.59%,胃苏组为40%,莫沙比利组为53.17%,健胃舒组与莫沙比利组比较有显著性差异(P<0.01)。结论:健胃舒分煎颗粒治疗FD疗效明显。     

Structure Characterization and Impact Effect of Al-Cu Graded Materials Prepared by Tape Casting

With the need of developing new materials, exploring new phenomenon, and discovering new mechanisms under extreme conditions, the response of materials to high-pressure compression attract more attention. However, the high-pressure state deviating from the Hugoniot line is difficult to realize by conventional experiments. Gas gun launching graded materials could reach the state. In our work, the corresponding Al-Cu composites and graded materials are prepared by tape casting and hot-pressing sintering. The microstructure and the acoustic impedance of the corresponding Al-Cu composites are analyzed to explain the impact behavior of Al-Cu graded materials. Computed tomographic testing and three-dimension surface profilometry machine results demonstrated well-graded structure and parallelism of the graded material. Al-Cu GMs with good parallelism are used to impact the Al-LiF target at 2.3 km/s using a two-stage light-gas gun, with an initial shock impact of 20.6 GPa and ramping until 27.2 GPa, deviating from the Hugoniot line.     

Research on Design and Performance of Self-Compacting Cement Emulsified Bitumen Mixture (CEBM)

To meet the needs of the road industry for maintenance operations, a new cement emulsified bitumen mixture (CEBM) with early-strength, self-compacting, and room-temperature construction characteristics was designed. The strength formation mechanism of CEBM was revealed with a scanning electron microscope (SEM) and the surface free energy (SFE) theory. The mechanical properties and road performance of the CEBM were investigated extensively. The results show that before the demulsification of emulsified bitumen, the SFE of the bitumen–aggregate–water three-phase system was reduced due to the replacement of the bitumen–aggregate interface with water. The adhesion work between the emulsified bitumen and the aggregate is negative, which means the adhesion between the emulsified bitumen and the aggregate will not occur spontaneously due to the existence of water. The liquid emulsified bitumen improves the workability of the mixture and ensures that the mixture can be evenly mixed and self-compacted. After demulsification, the work of adhesion between the residual bitumen and the aggregate is positive, which means residual bitumen and aggregate can bond spontaneously. In addition, the hydration products of cement and aggregate form a skeleton, and the emulsified bitumen film wraps and bonds the cement and aggregate together, creating strength. The emulsified bitumen, cement content, and curing conditions have significant effects on the stability of CEBM. The recommended dosage of emulsified bitumen and cement is 8% and 8–10%, respectively. This material integrates the hardening effect of cement and the viscoelastic performance of bitumen and has good workability, mechanical properties, and road performance. Therefore, the CEBM is technically feasible for application to bitumen pavement.     

Development and Primary Application of an Indirect ELISA Based on Rep Protein to Analyze Antibodies against Porcine Cocirvirus-like Virus (PCLV)

Porcine circovirus-like virus (PCLV) is a member of circovirus that contains a single-strand DNA genome, which may be one of the pathogens that causes diarrheal symptoms in pigs. The Rep protein encoded by the genome of PCLV may be responsible for viral genome replication. The development of serological detection methods for PCLV is of great necessity for clinical diagnosis, as well as epidemiological investigations. Therefore, this study attempted to build an indirect enzyme-linked immunosorbent assay (ELISA) to examine antibodies against PCLV based on the His-tagged recombinant Rep protein. Full-length PCLV Rep protein was induced and expressed in E. coli and was purified as an antigen to establish an ELISA detection kit. The purified Rep protein was used to inject into mice to produce specific antibodies. There was no cross-reaction of Rep-based ELISA with antisera against other porcine viruses. The intra-assay and inter-assay coefficient variations (CVs) were 0.644–8.211% and 0.859–7.246%, respectively, indicating good repeatability. The non-cross-reaction with TGEV, PRRSV and PCV2 testing showed high sensitivity and high specificity for this ELISA assay. A total of 1593 serum samples collected from different pig farms in Jiangxi Province were tested for anti-PCLV Rep antibodies, and 284 (17.83%) of the 1593 samples were Rep antibody positive. Altogether, the indirect ELISA detection tool developed in this study could be applied to examine serum of PCLV antibodies with good repeatability, high sensitivity and high specificity. In addition, field sample detection results suggested that the PCLV antibody has a low prevalence in pig populations in Jiangxi Province of China.     

内镜腹腔镜联合治疗胆总管结石150例报告

目的:探讨内镜腹腔镜联合治疗胆总管结石的价值。方法:回顾性分析150例胆总管结石患者经内镜腹腔镜联合治疗的临床资料。结果:本组患者全部治愈。120例患者接受了十二指肠乳头括约肌切开术(endoscopic sphicterotomy,EST)和十二指肠逆行胰胆管造影术(endoscapic retrograde cholangiopancreatography,ERCP);30例患者接受了腹腔镜胆囊切除术(laparoscopic cholecystectomy,LC)+腹腔镜胆总管切开取石术(laparoscopic choledocholithotomy T-tube drainage,LCTD)。结论:内镜腹腔镜联合治疗胆总管结石优于传统手术,在具备较高内镜、腹腔镜技术水平的条件下是安全可行的,且可作为首选方式。