TCD在锁骨下动脉盗血综合征诊断中的临床研究

目的探讨经颅多普勒超声(TCD)对锁骨下动脉盗血综合征(SSS)的诊断价值。方法回顾性总结100例经TCD检查诊断的锁骨下动脉(Sub-A)严重狭窄或闭塞患者。用经颅多普勒超声仪检测双侧椎动脉血流速度及频谱的变化,根据同侧椎动脉(Ip-VA)TCD检测的血流方向,将盗血程度分为:无盗血(Ip-VA完全正向)、Ⅰ度盗血(Ip-VA收缩期有切迹)、Ⅱ度盗血(Ip-VA收缩期反向,舒张期正向)和Ⅲ度盗血即完全盗血(Ip-VA完全反向)。盗血途径:基底动脉参与盗血(基底动脉呈盗血频谱)或不参与盗血两种。结果经TCD检查无盗血35例,Ⅰ度盗血20例,Ⅱ度盗血15例,Ⅲ度盗血30例。结论 TCD是诊断SSS及盗血程度的可靠方法,适用于临床诊断。     

腰骶段穴位对不稳定膀胱调节的穴位特异性研究

目的通过临床随机对照研究,观察针刺肾俞、会阳、肾俞加会阳对不稳定膀胱(USB)主要症状的影响,探索针刺不同穴位治疗USB的穴位特异性。方法将89例USB患者随机分为三组,即肾俞组、会阳组和肾俞加会阳组,以I-PSS积分为观察指标,分别在治疗3次、治疗6次后进行疗效评定和组间比较。结果(1)肾俞组、会阳组和肾俞加会阳组均能明显改善USB患者I-PSS总积分(P<0.05),会阳组和肾俞加会阳组疗效优于肾俞组(P<0.05)。(2)肾俞组、会阳组和肾俞加会阳组的有效率分别为50.0%、82.8%和90.0%。但会阳组和肾俞加会阳组要明显高于肾俞组(P<0.05)。结论会阳穴、肾俞穴、肾俞加会阳穴均能调节USB患者排尿紊乱症状,改善生活质量。并且以肾俞加会阳穴治疗效应最显著。     

Vertical split fracture of the vertebral body following oblique lumbar interbody fusion: A case report

Rationale:Oblique lumbar interbody fusion (OLIF) is an effective and safe surgical technique widely used for treating spondylolisthesis; however, its use is controversial because of several associated complications, including endplate injury. We report a rare vertebral body fracture following OLIF in a patient with poor bone quality.Patient concerns:A 72-year-old male patient visited our clinic for 2 years with lower back pain, leg radiating pain, and intermittent neurogenic claudication.Diagnoses:Lumbar magnetic resonance imaging revealed L4-5 stenosis.Intervention:We performed OLIF with percutaneous pedicle screw fixation and L4 subtotal decompressive laminectomy. We resected the anterior longitudinal ligament partially for anterior column release and inserted a huge cage to maximize segmental lordosis. No complications during and after the operation were observed. Further, the radiating pain and back pain improved, and the patient was discharged. Two weeks after the operation, the patient visited the outpatient department complaining of sudden recurred pain, which occurred while going to the bathroom. Radiography and computed tomography revealed a split fracture of the L5 body and an anterior cage displacement. In revision of OLIF, we removed the dislocated cage and filled the bone cement between the anterior longitudinal ligament and empty disc space. Further, we performed posterior lumbar interbody fusion L4-5, and the screw was extended to S1.Outcomes:After the second surgery, back pain and radiating pain in the left leg improved, and he was discharged without complications.Lesson:In this case, owing to insufficient intervertebral space during L4-5 OLIF, a huge cage was used to achieve sufficient segmental lordosis after anterior column release, but a vertebral body coronal fracture occurred. In patients with poor bone quality and less flexibility, a huge cage and over-distraction could cause a vertebral fracture; hence, selecting an appropriate cage or considering a posterior approach is recommended.     

基因重组毕赤酵母表达瑞替普酶过程中的酶活性分析

目的 研究毕赤酵母诱导表达瑞替普酶过程中的关键酶活性.方法 以摇瓶培养为研究对象,在用甲醇诱导后,连续取样,破碎菌体制成无细胞悬液,检测乙醇氧化酶、甲醛脱氢酶、PDC、G-6-PD、ID、α-KGD和SD的活性.结果 乙醇氧化酶比活在0～6 h逐渐增加,在第6h达到最大44.5 U/mg蛋白.随后迅速下降.在第24～48 h有所回升,然后又逐渐降低.FAD比活在第0～48h逐渐增加.在第48h达到最大值6.72U/mg蛋白,随后逐渐降低.直至放瓶.G-6-PD比活在第2h～6h逐渐增加,在第6～24h逐渐降低,在第24～48h逐渐升高,48h后又逐渐降低直至放瓶.PDC比活在第0～6h逐渐降低,随后略有升高的趋势.ID、α-KGD、SD的活性变化有相似趋势.在前6 h酶活均迅速下降,在第6～24 h缓慢下降,随后ID活性继续缓慢下降,而α-KGD和SD活性在第2～48 h逐渐升高,在第48 h均达到最高值,然后又逐渐降低,直至放瓶.结论 根据酶活变化规律,可将整个诱导期分为4个阶段:第1阶段为诱导0～6 h,是甲醇适应期;第Ⅱ阶段为诱导6～24h,是快速生长期;第Ⅲ阶段为诱导24～48h,是产物积累期;第Ⅳ阶段为诱导48～72h,是代谢缓慢期.在甲醇适应期,甲醇完全氧化代谢流占主导地位.在快速生长期和产物积累期,代谢流逐渐向糖酵解途径和TCA循环途径迁移.     

海军官兵心理韧性及应对方式对职业倦怠的影响

目的 探讨海军官兵心理韧性及应对方式对职业倦怠的影响,为促进海军官兵的心理健康提供依据。方法 使用职业倦怠量表（MBI）、特质应对方式问卷（TCSQ）、心理韧性问卷（CD-RISC）对588名海军官兵进行问卷调查。结果 海军官兵职业倦怠总分为（33.34±9.93）分,其中,情绪耗竭维度分为（10.40±4.31）分,去个性化维度分为（7.28±3.05）分,低职业效能维度分为（15.66±6.62）分,积极应对得分为（36.40±7.21）分,消极应对得分为（26.06±9.92）分,心理韧性平均分为（89.79±19.27）分。职业倦怠与消极应对之间具有显著的相关性（r=0.45,P<0.05）,与心理韧性具有显著的负相关(r=-0.46,P<0.05)。线性回归结果表明,影响职业倦怠的因素为兵种（β=0.28）和心理韧性（β=-0.32）。结论 职业倦怠与职业环境（兵种）与消极应对相关,心理韧性对其有预防保护作用。     

Biocompatibility assessment of nanomaterials for environmental safety screening

In view of the extensive use of nanoparticles in countless applications, a fast and effective method for assessing their potential adverse effects on the environment and human health is extremely important. At present, in vitro cell‐based assays are the standard approach for screening chemicals for cytotoxicity because of their relative simplicity, sensitivity, and cost‐effectiveness compared with animal studies. Regrettably, such cell‐based viability assays encounter limitations when applied to determining the biological toxicity of nanomaterials, which often interact with assay components and produce unreliable outcomes. We have established a cell‐impedance‐based, label‐free, real‐time cell‐monitoring platform suitable for use in a variety of mammalian cell lines that displays results as cell index values. In addition to this real‐time screening platform, other traditional cytotoxicity assays were employed to validate cytotoxicity assessments. We suggest that the cell impedance measurement approach is effective and better suited to determining the cytotoxicity of nanomaterials for environmental safety screening. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1170–1182, 2017.     

用多重PCR方法鉴别不同卡介苗菌株

目的用多重PCR方法鉴别不同卡介苗菌株。方法根据卡介苗DNA的6个目的区域的特点设计引物,进行多重PCR扩增,然后电泳检测分析。结果多重PCR方法能够鉴别不同卡介苗亚株。结论应用多重PCR方法鉴别卡介苗菌株具有专一性好、重复性高、快速简便等优点。     

用于卡介菌多糖核酸制剂效力实验的小鼠模型的建立

目的  建立用于卡介菌多糖核酸制剂效力实验的小鼠模型。方法  选用猪血清、牛血清白蛋白、卵白蛋白作为致敏原,腹腔注射BALB/c小鼠以建立Ⅰ型变态反应动物模型,并通过在卡介菌多糖核酸制剂效力实验中应用建立的小鼠模型来验证其可行性。结果  猪血清、牛血清白蛋白和卵白蛋白均可作为致敏原致敏BALB/c小鼠,但牛血清白蛋白和卵白蛋白的致敏效果优于猪血清。卡介菌多糖核酸制剂效力实验显示,牛血清白蛋白致敏小鼠建立的动物模型可更好地反映卡介菌多糖核酸制剂的效力。结论  牛血清白蛋白致敏小鼠建立的动物模型可用于卡介菌多糖核酸制剂效力实验。