自体表皮细胞培养与异体真皮组合应用研究

严重烧伤病人皮肤修复中主要未解决的问题是真皮的替代。动物实验结果表明，异体皮移植后５天，用自体培养表皮细胞膜片覆盖真皮床，１４天后复合皮成活率是８４．６％±２．４％。组织学检查证实表皮已形成了复层结构，可见基底层、颗粒层和角质层。临床应用中，异体皮移植后１０天，去除异体表皮覆盖病人的自体培养表皮，３５天后未见排斥征象，异体真皮促进了培养表皮的分层、成熟和完整，组织学检查证实表皮细胞的边缘清楚，已分化形成颗粒层和角质层，真皮多细胞，已血管化，但表皮嵴缺乏。     

碱性成纤维细胞生长因子（bFGF）加速猪背部创伤修复的实验研究

实验采用猪背部创伤模型，观察重组的碱性成纤维细胞生长因子（ｂＦＧＦ）对创伤修复的作用。在无菌条件下用特制打孔器在猪背部切割皮肤，形成２．５ｃｍ２的园型创面。将１５０个创面分成５组，分别以ｂＦＧＦ（６０ｕ／ｃｍ２创面），ｂＦＧＦ（（６０ｕ／ｃｍ２创面）加ＳＤ－Ａｇ、ＳＤ－Ａｇ、生理盐水以及不治疗等方法处理，每间隔一天换药一次。实验结果表明，ｂＦＧＦ具有显著促进肉芽组织生长、加速创面再上皮化率以及增加创面愈合组织抗张力强度等作用。ＦＧＦ显著促修复效应的确切机制尚不完全清楚，可能包括直接与间接效应两方面。     

酶组化显示睾丸LDH－X方法的再探讨

对小鼠睾丸冰冻切片ＬＤＨ－Ｘ的显示方法作了进一步探讨。实验表明，以Ｌ－２－羟基－３－甲基戊酸（ＨＭＶ）为特异性底物，能相当有效地显示睾丸ＬＤＨ－Ｘ的分布，且该底物的配制方法简便易行。在显示ＬＤＨ－Ｘ的孵育液中采用萘酚蓝（ＮＢ）作中间递电子体较吩嗪甲基硫酸酯更具优越性。     

Central circulation in rats with different tolerance to acute blood loss

Study of the dynamics of cardiac output in rats with different tolerance to acute massive blood loss showed that the pumping ability of the heart remains intact during the entire posthemorrhagic period in all high-resistant and in 65% low-resistant rats. In 35% rats that were low-resistant to blood loss, the cardiac output deficiency syndrome developed after cessation of bleeding against the background fall in arterial pressure and a decrease in the hepatic blood flow, which are the signs of rapid variant of the dysfunction produced by acute blood loss. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 10, pp. 384–388, October, 1998     

Midazolam in treatment of various types of seizures in children

Midazolam is a recently developed water-soluble benzodiazepine that shares anxiolytic, muscle relaxant, hypnotic and anticonvulsant actions with other members of this class. There are limited studies that midazolam can be used successfully to treat seizures in adults and children. In this study, 0.2 mg/kg intramuscular (IM) midazolam was administered to 11 children (eight boys and three girls), aged 3 days to 4 years (mean age 1.8±1.4 years), with seizures of various types. In all but one child, seizures stopped in 15 s–5 min after injection. No side effects were observed. These results suggest that IM administration of midazolam may be useful in a variety of seizures during childhood, especially in case of intravenous (IV) line problem.     

Effects of specific antibodies on the ultrastructure of mouse oocytes

The effects of antiovarian antiserum and monoclonal antibodies to the oolemma antigens on the ultrastructure of mouse oocytes and their microenvironment are studied. The antioolemma monoclonal antibodies cause more pronounced degenerative changes in the oocyte that in its microenvironment. Antiovarian antiserum induces greater changes in the microenvironment than in the oocyte. Changes induced in the oocyte by the antiserum are secondary relative to changes occurring in the microenvironment, while changes observed in the oocyte treated with monoclonal antibodies are primary. Translated fromByulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 123, No. 1, pp. 115–119, January, 1997     

蝮蛇抗栓酶与肝素抗凝治疗小儿肾病的疗效比较

本文对60例小儿肾病患儿随机分为3组,蝮蛇抗栓酶治疗组,肝素治疗组,常规治疗组(对照组),结果总有效率分别为95％,90％,60％。蝮蛇抗栓酶组与对照组比较,差异有极显著性(P<0.01),肝素组与对照组比较,差异有显著性(P<0.05)。结果提示抗凝治疗对小儿肾病高凝状态有明显改善作用,能增加临床疗效。蝮蛇抗栓酶抗凝效果优于肝素。     

IGF-Ⅰ及IGF-Ⅱ受体反义基因对SMMC-7721肝癌细胞株增殖能力的影响

目的；研究胰岛素样生长因子Ⅰ受体、Ⅱ受体（IGF－ⅠR，IGF－ⅡR）反义基因对SMMC－7721细胞体外增殖能力的影响。方法：应用反义技术，在已将IGF－ⅠR反义基因转染入SMMC－7721肝癌细胞株的基础上（获得ⅠR－AS细胞），继续利用IGF－ⅡR反义基因真核表达系统，通过DOTAP^TM脂质体介导的转技术，将IGF－ⅡR反义基因导入该细胞，经G418及潮霉素双重筛选获得稳定表达IGF－Ⅰ,IGF－ⅡR反义基因的SMMC－7721细胞（Ⅰ、ⅡR－AS细胞）。结果：光镜，电镜下反义转染细胞形态学上肿瘤性有一定减弱；在软琼脂培养基中生长能力低下（P＜0．01），反义G418抗性转染细胞克隆形成能力低下（P＜0．05）；在细胞生长曲线上与对照组相比有轻度下降趋势。结论：IGF－ⅠR，IGF－ⅡR反义基因对SMMC－7721肝癌细胞株的恶性增殖有明显抑制作用。     

120例感染患者骨髓涂片细胞形态观察

目的 :探讨感染患者骨髓细胞的形态学变化。　方法 :对 12 0例确诊有感染性病症的患者进行骨髓细胞形态分析。　结果 :感染患者骨髓中网状细胞增高者占 15 % ,伴吞噬细胞增高者占 8.3% ,有异型淋巴细胞出现者占9.2 % ,浆细胞相对增高者占 14 .2 %。　结论 :部分感染患者骨髓内既有网状细胞、吞噬细胞及浆细胞的反应性增生 ,亦可伴有异型淋巴细胞的增生