Effects of rat urotensin Ⅱ on coronary flow and myocardial eNOS protein expression in isolated rat heart

AIM: To examine the effects of urotensin Ⅱ, a recently discovered endogenous peptide, on coronary flow (CF), cardiac function, and endothelial nitric oxide synthase (eNOS) expression in isolated rat hearts. METHODS: Heart was isolated and perfused retrogradely via the aorta in Langendorff mode. Rat urotensin Ⅱ was administered in the perfusion solution. The eNOS content in myocardium was determined by Western blot. RESULTS: Rat urotensin Ⅱ had no effect on the heart rate, left ventricular systolic pressure, left ventricular end-diastolic pressure, or ±dp/dtmax. While rat urotensin Ⅱ dose-dependently increased CF. CF was increased by 11.43 %, 6.67 %, 6.62 %, 6.56 %, 6.36 %, and 5.86 % respectively in a time-dependent manner at 5, 10, 15, 20, 25, and 30 min after injection of rat urotensin II 6.66×10-2μg. The maximal effect on CF was found at 5 min following urotensin II administration. NG-nitro-L-arginine methyl ester (L-NAME) did not prevent the increased CF in response to urotensin II. Rat uroten     

大黄复方液体喷雾剂中挥发油微乳与相图的应用

目的:用三元相图法优选大黄复方液体喷雾剂中挥发油形成微乳的最佳处方方法:选泊洛沙姆-108为乳化剂,乙醇为助乳化剂,姜黄、金银花挥发油为油相,借助三元相图的研究手段确定乳化剂和助乳化剂的最佳用量结果:泊洛沙姆-108与乙醇量的比为1:10,处方量挥发油形成微乳,稳定分散于制剂中.结论:借助三元相图研究中药复方液体制剂中挥发油形成微乳的最佳处方,为液体制剂中挥发油的稳定分散问题提供了一条捷径;通过微乳增加中药复方制剂中难溶性挥发油类药物的溶解度,提高中药复方液体制剂的稳定性.     

不同启动子指导的HSV1-sr39TK/GCV系统对小鼠淋巴细胞的敏感性研究

目的观察慢病毒载体中不同启动子指导的突变型单纯疱疹病毒1型胸苷激酶(HSV1-sr39TK)基因在小鼠T淋巴细胞内的表达差异,并进一步比较对丙氧鸟苷(ganciclovir,GCV)的敏感性。方法用亚克隆技术将PCR扩增的HSV1-sr39TK基因分别连接至慢病毒载体不同启动子后,然后在HSV1-sr39TK基因后以内部核糖体进入位点(internal ribosome entry sites,IRES)连接绿色荧光蛋白(green fluorescent pro-tein,GFP)报告基因;采用三质粒包装系统包装病毒,将病毒感染刀豆蛋白A(concanavalin,ConA)激活的小鼠淋巴细胞,分别用流式细胞术、RT-PCR法鉴定HSV1-sr39TK和GFP基因在小鼠淋巴细胞的表达,用Cell Counting Kit-8(CCK-8)法观察感染不同病毒的淋巴细胞对前体药物GCV的敏感性。结果不同启动子指导的HSV1-sr39TK及GFP基因均可在小鼠淋巴细胞表达,巨细胞病毒(cytomegalovirus,CMV)启动子驱动表达能力最强,磷酸甘油激酶(phosphoglycerokinase,PGK)启动子最弱。感染含CMV启动子病毒的淋巴细胞对GCV的IC50值最小。结论在小鼠淋巴细胞内CMV启动子驱动的慢病毒载体HSV1-sr39TK基因表达最强,对前体药物GCV敏感性最高。     

增殖细胞核抗原突变体的稳定高表达对HeLa细胞顺铂敏感性的影响

目的 建立稳定高表达增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)或突变体PC-NA(mutant PCNA,mPCNA)目的蛋白的宫颈癌细胞系HeLa细胞,观察PCNA在顺铂诱导的DNA损伤修复中的作用.方法 采用显性负突变策略,通过构建重组人PCNA或mPCNA的真核表达质粒pCDNA3.1/V5-His A-PCNA(His-PCNA)和pCDNA31/V5-His A-mPCNA(His-mPCNA),稳定转染到HeLa细胞中,G418筛选建立稳定高表达目的蛋白的HeLa细胞系;Western印迹法检测蛋白的表达情况;MTT法测定顺铂损伤后不同细胞系的细胞存活率.结果 真核表达质粒经酶切、测序分析与实验设计完全一致;稳定建系后,Western印迹结果显示在相应位置可见清楚的目的条带;MTT结果显示稳定高表达突变体PCNA的细胞系与对照组相比,其细胞存活率呈明显下降趋势.结论 成功构建了真核表达质粒His-PCNA和His-mPC-NA;建立了稳定高表达该质粒的HeLa细胞系;稳定高表达突变体PCNA的细胞系对顺铂损伤更敏感.     

胸主动脉腔内修复术采用裙边型烟囱支架（Longuette™）的PATENCY临床试验研究结果

背景与目的 烟囱技术是胸主动脉腔内修复术（TEVAR）中重建左锁骨下动脉（LSA）的方法,Ⅰa型内漏是其主要并发症。裙边型烟囱支架（Longuette?）专为烟囱技术设计,用于重建LSA时降低Ⅰa型内漏。为评估Longuette?烟囱支架联合TEVAR治疗累及LSA的Stanford B型主动脉夹层（TBAD）的疗效,笔者开展了前瞻性、多中心临床试验（PATENCY临床试验）。本研究总结PATENCY临床试验的1年结果和经验。方法 2018年10月—2022年3月,全国26家血管外科中心参与PATENCY临床试验,共纳入150例符合标准的TBAD患者。所有患者均在TEVAR术中采用Longuette?烟囱支架重建LSA。评估患者围手术期和术后12个月的临床效果和不良事件,并分析患者术后1年累积生存率、LSA通畅率和无内漏率。结果 患者年龄30~77岁,平均（54.48±11.12）岁,138例（92.0%）患者合并高血压病;急性、亚急性和慢性TBAD分别占74.7%,17.3%和8.0%。124例（82.7%）患者采用全身麻醉。手术成功率为99.33%（149/150）,手术时间（91.67±41.47）min,X线暴露时间（31.36±16.71）min,手术出血量为（71.55±60.40）mL。围手术期内漏发生率为5.33%（8/150）,包括Ⅰ型6例、Ⅱ型1例、Ⅳ型1例;1例（0.67%）患者发生Longuette?烟囱支架急性闭塞,再次实施腔内手术后恢复通畅;1例（0.67%）患者术后发生急性脑梗死;2例（1.33%）患者术后发生逆撕的Stanford A型主动脉夹层,其中1例术后3周死亡。术后30 d死亡2例（1.33%）。142例患者进行了密切随访,随访时间为11.67（5~16）个月。无主动脉支架和Longuette?烟囱支架移位。2例Ⅰ型内漏患者分别于术后6个月和1年进行了再次介入栓塞手术治疗,术后1年随访仍有6例患者有轻微的内漏持续存在,患者TBAD假腔保持稳定,无明显症状,均予以保守治疗。Longuette?烟囱支架内狭窄和闭塞分别发生1例和2例,逆撕的Stanford A型主动脉夹层患者1例,假腔增大,患者均无明显症状,予以保守治疗。无发生脑卒中、截瘫、左上肢缺血等并发症。术后12个月累积生存率、LSA通畅率、无内漏率分别为97.96%、97.96%和91.91%。结论 采用Longuette?烟囱支架在TBAD腔内治疗中重建LSA简便、安全、有效,其能够有效降低围手术期Ⅰa型内漏的发生率,为微创治疗主动脉弓部病变提供新的治疗方式。     

Two important factors affecting the prognosis of patients treated with sorafenib followed by regorafenib for advanced hepatocellular carcinoma

ObjectiveTo investigate the effects of hand–foot syndrome (HFS) and fatigue on disease progression and survival in patients treated with sorafenib followed by regorafenib for advanced hepatocellular carcinoma.MethodsA retrospective analysis of patients with advanced hepatocellular carcinoma treated with sorafenib in our hospital from 1 October 2018 to 31 October 2021 was performed, and clinical and pathological data and follow-up results were obtained. Patients were divided into groups according to the severity of HFS and fatigue. Survival analysis among the groups was performed using the Kaplan–Meier method, continuous variables were analyzed using the t-test, and factors associated with survival were evaluated using multivariate Cox regression analysis.ResultsThe study included 150 men and 23 women with a mean age of 60.77 years (range: 40–85 years). The median overall survival (OS), progression-free survival (PFS), and time to tumor progression (TTP) increased with increasing severity of HFS. Conversely, the median OS, PFS, and TTP decreased with increasing severity of fatigueConclusionHFS and fatigue were independent risk factors affecting TTP, PFS, and OS among patients treated with sorafenib followed by regorafenib for advanced hepatocellular carcinoma.     

护理风险预警机制在慢性乙型病毒性肝炎肝衰竭病人护理中的应用效果

目的:探讨护理风险预警机制在慢性乙型肝炎肝衰竭病人护理中的应用效果。方法:选取2018年1月—2019年12月我院收治的104例慢性乙型肝炎肝衰竭病人,按照病人入院顺序,并采用单双号抽签方式分为对照组和观察组,各52例。对照组给予常规护理干预,观察组临床护理中引入护理风险预警机制,对比两组肝功能指标、并发症发生情况、护理满意度、护理质量评分、护理缺陷评分及住院时间。结果:干预后,两组肝功能指标均有所改善,且观察组下降幅度较对照组大(P<0.001);观察组与对照组并发症发生率分别为7.69%、25.00%,观察组与对照组总满意度分别为96.15%、76.92%,观察组病人护理质量评分高于对照组,护理缺陷评分及住院时间低于对照组,差异均有统计学意义(P<0.05)。结论:将护理风险预警机制用于慢性乙型肝炎肝衰竭病人的临床护理中,有利于改善病人肝功能指标,减少并发症及护理缺陷,提高护理满意度,提升护理质量,缩短病人住院时间。     

Altereporenes A–E,five epoxy octa-hydronaphthalene polyketides produced by an endophytic fungus Alternaria sp. YUD20002

Five previously undescribed epoxy octa-hydronaphthalene polyketides, altereporenes A–E (1–5) were isolated from rice culture of the endophytic fungus Alternaria sp. YUD20002 derived from the tubers of Solanum tuberosum. Their structures were determined on the basis of comprehensive spectroscopic analyses, while the absolute configurations were elucidated by the comparison of experimental and calculated specific rotations. Meanwhile, the antimicrobial, cytotoxic, anti-inflammatory and acetylcholinesterase inhibitory activities of compounds 1–5 were also investigated.

Five previously undescribed epoxy octa-hydronaphthalene polyketides, altereporenes A–E (1–5) were isolated from rice culture of the endophytic fungus Alternaria sp. YUD20002 derived from the tubers of Solanum tuberosum.     

浸润型肺结核CT与临床疗效对比分析

目的 评价初治浸润型肺结核患者的临床疗效和螺旋CT影像学特点的对比研究。方法 116例浸润型肺结核患者,采用抗结核治疗,分别在1,2,4,8和12个月进行对比研究。结果 在1,2和4个月的对比研究中,螺旋CT影像学表现的好转情况明显低于临床表现（P〈0．05）,而8和12个月对比结果两者表现相同。结论 CT表现晚于临床症状的转归。说明即使临床结核中毒症状好转或消失,也不应该忽视结核的化学治疗。     

血浆游离双链DNA在原发性肝癌中的诊断价值

目的:探讨血浆中游离双链DNA(cell-free double-stranded DNA,cf-dsDNA)在原发性肝癌(primary hepa-tic carcinomas,PHC)诊断中的应用.方法:招募62例PHC患者和41例良性肝病患者以及45例健康对照,填写信息登记表.抽取治疗前患者和健康对照的空腹血样,...