Determination of antigen-dependent activity of human lung cancer transfer factor by H3-leucine leucine leukocyte adherence inhibition assay]

This report is to demonstrate the antigen-dependent activity of human lung cancer transfer factor (Sp-TF). Sp-TFM was prepared from spleen of mice immunized with the human lung cancer cell line A549. [3H]-leu leukocyte adherence inhibition assay ([3H]-leu-LAI) was modified to identify activity of Sp-TFM. Leukocytes obtained from non-immunized mice were divided into eight groups as follows: 1. Control without TF or antigen; 2. Sp-TFM and antigen of cell line A549 (A549 Ag); 3. Sp-TFM alone; 4. Sp-TFM and ascitic tumor cell H22 antigen of mice (H22Ag); 5. Sp-TFM and antigen of human gastric cancer cell (HGCCAg); 6. Sp-TFM and antigen of human normal lung tissue (NLTAg); 7. Nor special TF of mice (N-TFM) and A549Ag; 8. A549Ag alone. When normal leukocytes were incubated with Sp-TFM and A549 antigen, the leukocytes adherence inhibition index (LAII) was significantly higher than those of the other groups. The different LAII of Sp-TFM to A549Ag and other experimental groups were highly significant (P less than 0.001). The results demonstrated that Sp-TFM could transfer specific cell mediated immunity to non-immune leukocytes. The TF prepared from spleen of goat immunized with antigen from lung cancer of patients (Sp-TFG) showed antigen specific activity as well as Sp-TFM.     

Computerized tomography based on DEI refraction information.

Conventional computerized tomography (CT) technique is based on the absorption contrast. In recent years, X-ray phase-contrast CT (PCCT) has been developing rapidly. It uses the phase information to reconstruct the object and provide high contrast and spatial resolution. Diffraction enhanced imaging (DEI) method is one of the three phase-sensitive X-ray imaging techniques. DEI method employs an analyzer crystal to extract the object's refraction information which can be used for CT. However, when DEI refraction CT is combined with the conventional CT algorithm, it should be satisfied that the refraction information of an arbitrary point in the object is invariable at every projection view. In this paper, the invariance condition of refraction information is analyzed and two feasible methods are provided for reconstruction. Using these two methods, two samples of weak absorption are reconstructed with the experimental data obtained at Beijing Synchrotron Radiation Facility (BSRF).     

用酶联免疫吸附术观察向附睾注射鱼肝油酸钠后抗精子抗体的变化

本研究用酶联免疫吸附术观察8只家兔在附睾注射鱼肝油酸钠后血清及精浆中的抗精子抗体。血清抗精子抗体阳性率为37.5～50.0%,最早检出率为术后2个月,6～7月达高峰。精浆抗精子抗体阳性率为25%,最早检出率为术后4个月,且此时己达高峰。与输精管结扎资料相比,发现血清抗精子抗体检出率偏低,而精浆相应抗体偏高,表明向附睾注射鱼肝油酸钠引起的免疫反应有别于结扎,以生殖道局部为主。     

Smooth muscle relaxant effect of dehydroindicolactone

Dehydroindicolactone, a novel linear furanocoumarin with an 8-substituted side chain carrying a 5-member ring lactone, showed spasmolytic activity in a number of smooth muscle preparations. The spasmolytic activity seems to lie in its ability to block the dihydropyridine-sensitive calcium channel.     

加减化裁随证治之——何任治疗癌症学术经验探究(下)

癌症为深危重疾,对于绝大部分癌症患者,临床普遍采用手术、中医、化疗、放疗、生物等综合方法进行治疗。由于癌症的种类、所处阶段、先前采用的治疗方法以及病人体质等等的不同,求诊时病人的证     

A novel DRB1*09 allelic sequence in the Jing ethnic minority of China.

A new DRB1 allele, DRB1*0902, has been identified in an individual of the Jing ethnic minority. Its sequence was confirmed by sequencing of PCR products and clones. This allele differed by three nucleotides from DRB1*09012 at positions 157, 161 and 166, and resulted in amino acid motif substitution from VAES to DAEY.     

美国医院的集团化运作

自1923年以来的这段时间,是医院发展史上最具探索性的时期.许多医院因为严峻的经济大环境而艰难度日,住院率低下,公益负担日增,融资渠道日益萎缩,私立机构的状况更是让人揪心,前景看起来一片黯淡.然而所幸的是,每次经济危机也带来了新的观念和方法.医院在逆境中反思,逐渐改变旧有的行为方式,引入商业运营模式,学会当家理财.