蓝-黄视野计与标准的白-白视野计对早期原发性开角型青光眼视野的对比研究

目的　评价蓝 -黄视野计 (blue- on- yellow perim etry,B/ Y)又称短波长视野计 (short- wavelength auto-mated perim etry,SWAP)与标准的白色视野计 (white- on- white perimetry,W/ W)在早期青光眼视野损害诊断中的意义。方法　利用 Hum phrey- - 75 0型全自动视野计 C- 30 - 2全阈值检测程序对 2 0例 (4 0只眼 )正常人、2 0例 (36只眼 )可疑青光眼、18例 (32只眼 )确诊的早期原发性开角型青光眼 (primary open- angle glaucoma,POAG)分别进行 W/ W与 B/ Y的视野检查。结果　正常人、可疑青光眼、早期青光眼 B/ Y检测的全视网膜光敏感度均值 (MS)低于 W/ W检测的结果 ,两者有显著性意义 (t=11.6 8,P <0 .0 1;t=14 .0 1,P <0 .0 1;t=14 .6 8,P <0 .0 1)。B/ Y检测光敏感度缺损均值 (MD)与 W/ W检测的结果 ,正常人组无显著性意义 (t=1.0 4 ,P <0 .0 1) ,其它两组有显著性意义 (t=4 .88,P <0 .0 1;t=3.378,P <0 .0 1)。W/ W检查正常人组与可疑性青光眼的 MS差别无显著意义 (t=2 .5 4 ,P >0 .0 1) ,B/ Y有显著意义 (t=5 .5 7,P <0 .0 1)。两组的 MD差别均有显著意义 (t=3.16 ,P <0 .0 1,t=6 .2 6 ,P <0 .0 1) ;W/ W与 B/ Y检查正常人组与早期青光眼组的 MS差别有显著意义 (t=6 .4 7,P <0 .0 ;t=10 .19,P     

宫颈鳞癌中Cyclin E的表达和HPV16感染的相关研究

目的探讨Cyclin E在宫颈鳞状细胞癌中的表达及其与HPV16感染的关系.方法应用免疫组化S-P法对34例宫颈鳞状细胞癌组织进行Cyclin E与HPV16的检测,并与宫颈不典型增生和正常宫颈组织进行比较.结果正常宫颈组织无Cyclin E表达,宫颈不典型增生组织仅有少量袁达(11.11%),宫颈鳞状细胞癌有58.52%表达(P＜0.001),且与HPV16感染有明显相关性(P＜0.05).结论高危HPV感染是宫颈癌的重要病因,HPV感染通过多种途径改变了细胞的周期性调控,诱发细胞周期GI-S期检测点异常,引起细胞恶性增生,HPV感染与Cyclin E异常表达在宫颈癌的发生发展中起重要作用.     

The role of electrochemical biosensors in SARS-CoV-2 detection: a bibliometrics-based analysis and review

The global pandemic of COVID-19, which began in late 2019, has resulted in extremely high morbidity and severe mortality worldwide, with important implications for human health, international trade, and national politics. Severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the primary pathogen causing COVID-19. Analytical chemistry played an important role in this global epidemic event, and detection of SARS-CoV-2 even became a part of daily life. Analytical chemists have devoted much effort and enthusiasm to this event, and different analytical techniques have shown very rapid development. Electrochemical biosensors are highly efficient, sensitive, and cost-effective and have been used to detect many highly pathogenic viruses long before this event. However, another fact is that electrochemical biosensors are not the technology of choice for most detection applications. This review describes for the first time the role played by electrochemical biosensors in SARS-CoV-2 detection from a bibliometric perspective. This paper analyzed 254 relevant research papers up to June 2022. The contributions of different countries and institutions to this topic were analyzed. Keyword analysis was used to explore different methodological attempts of electrochemical detection techniques. More importantly, we are trying to find an answer to the question: do electrochemical biosensors have the potential to become a genuinely employable detection technology in an outbreak of infectious disease?

This review describes for the first time the role played by electrochemical biosensors in SARS-CoV-2 detection from a bibliometric perspective.     

双黄连注射剂不良反应的原因及分析

目的观察、分析和总结双黄连注射剂的不良反应和应对措施。方法通过查阅1994～2008年期间国内公开发行的医药卫生期刊和国家食品药品监督管理局药品不良反应监测中心《信息通报》有关药品不良反应的资料进行分析。结果11083例双黄连注射剂不良反应的病例报告中出现严重不良反应的有306例,涉及158家企业的产品。性别、年龄段没有明显差异;以用药当天出现不良反应为主,严重不良反应的治愈或好转率〉90%,总死亡率高达5.9%,大多数病例对疾病本身影响不大。结论应根据双黄连注射液出现不良反应的可能原因,采取有针对性的安全防治措施。     

UPLC法测定一捻金中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的含量

目的:建立超高效液相色谱法测定一捻金中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1含量的方法。方法:色谱柱:ZORBAX Eclipse Plus C18柱(2.1 mm×50 mm,1.8μm);流速:0.21 ml·min-1;柱温:30℃;流动相:乙腈-水梯度洗脱;检测波长:203 nm;进样量:1μl。结果:人参皂苷Rg1在浓度为0.020 3~0.303 9 mg·ml-1时线性关系良好(r=0.999 6),平均回收率为99.05%,RSD为1.3%(n=6);人参皂苷Re在浓度为0.020 2~0.302 7 mg·ml-1时线性关系良好(r=0.999 8),平均回收率为101.31%,RSD为1.1%(n=6);人参皂苷Rb1在浓度为0.020 3~0.305 1 mg·ml-1时线性关系良好,r=0.999 8,平均回收率为100.71%,RSD为0.9%(n=6)。结论:该方法简单、准确,可同时测定3种人参皂苷的含量,可作为一捻金的质量控制。     

实行教考分离是提高中医教学质量的有效措施

教学质量的高低对于学校来说可谓生死攸关的大事.为了提高教学质量,各学校均进行了积极的探索,除了对教学方法、课程设置、教材建设和师资队伍建设等方面进行改革外,实行教考分离已被认为是保证和提高教学质量的有效措施.教考分离能否取得成功的关键是题库建设.没有高质量的题库用于考试就无法形成高要求的标准,也就难以达到教考分离的目的.笔者结合我校题库建设的实际,谈一些认识.     

清气凉营注射液对风温肺热病患者血清促炎、抗炎因子的调节作用

目的观察清气凉营注射液对风温肺热病（热在肺卫证）患者体内血清促炎及抗炎因子的调节作用及机制。方法将风温肺热病（热在肺卫证）60例患者随机分为两组,治疗组给予清气凉营注射液静滴,对照组予以双黄连注射液静滴,疗程均为3d;比较两组疗效及血清促炎、抗炎因子的变化情况。结果治疗组疗效优于对照组,其血清促炎因子（TNF-α、IL-6、IL-8）下降幅度及抗炎因子（IL-4、IL-10）上升幅度均大于对照组。结论清气凉营注射液作用机制可能与其调节细胞因子、平衡促炎／抗炎因子有关。     

Clinical trial on the role of tuina in rehabilitation therapy following total hip replacement

目的：观察推拿在全髋关节置换术后康复治疗中的临床疗效。方法：将60例患者随机分成治疗组和对照组。治疗组30例进行推拿结合康复治疗,对照组30例进行单纯康复治疗。两组均治疗2星期。分别观察两组患者术后7天、术后2星期、术后6星期的改良人工髋关节Harris量表及汉密尔顿焦虑量表（Hamilton Anxiety Rating Scale,HAMA）。结果：Harris量表评分总分,两组组内患者不同时间点多重比较差异有统计学意义（P〈0．05）;术后1天、术后7天及术后6星期两组间比较,差畀均无统计学意义（A,0．05）;术后2星期两组间比较差异有统计学意义（P〈0．05）。HAMA量表两组组内患者不同时间点多重比较差异有统计学意义（P〈0．05）;术后1天两纽间评分无显著性差异（P〉0．05）;术后7天、术后2星期、术后6星期两组间评分均有显著性差异（P〈0．05）。结论：单位时间内,推拿结合康复治疗对全髋关节置换术后患者的疼痛、关节活动范围及焦虑等方面的改善作用优于单纯康复治疗。     

Value of combined detection of serum amyloid A,C-reactive protein and procalcitonin in differential diagnosis of respiratory tract infection in children of China

ObjectiveTo explore the diagnostic value of combined detection of serum amyloid A (SAA), C-reactive protein (CRP) and procalcitonin (PCT) in children with bacteria or non-bacterial respiratory tract infection.Methods200 children with respiratory tract infections diagnosed in our hospital were included in the study. According to the results of the aetiological examination, they were divided into bacterial infection group and non-bacterial infection group. At the same time, 100 healthy children admitted to the hospital for physical examination during the same period were selected as the healthy subjects control group. Changes in serum SAA, PCT and CRP in three groups were compared. Comparison of a positive rate of the single index and combined detection were performed. Children with bacterial infections were treated with conventional antibiotics. The changes in serum SAA, PCT and CRP in the infection group before and after treatment were compared. The efficacy of SAA, PCT and CRP alone and in combination was compared.ResultsThe serum SAA, PCT and CRP levels in the bacterial infection group were higher than those in the non-bacterial infection group and healthy children, and the differences were statistically significant. The positive detection rates and combined detection rates of serum SAA, PCT and CRP in the bacterial infection group were higher than those in the non-bacterial infection group and the healthy subject''s control group. After conventional antibiotic treatment, serum SAA, PCT and CR levels in children with bacterial infection were significantly decreased.ConclusionThe combined detection based on SAA, CRP and PCT can effectively identify and diagnose respiratory tract infection in children, providing a certain reference for the promotion of the diagnostic scheme.

Key messages

• Serum SAA, PCT and CRP were highly expressed in children with respiratory tract infection, and the expression level was the highest in children with bacterial pneumonia.
• The combined detection of serum SAA, CRP and PCT indicators have higher diagnostic efficiency and can effectively make a differential diagnosis of respiratory tract infection in children.

     

Anti-tumor pharmacology of natural products targeting mitosis

Cancer has been an insurmountable problem in the history of medical science. The uncontrollable proliferation of cancer cells is one of cancer’s main characteristics, which is closely associated with abnormal mitosis. Targeting mitosis is an effective method for cancer treatment. This review summarizes several natural products with anti-tumor effects related to mitosis, focusing on targeting microtubulin, inducing DNA damage, and modulating mitosis-associated kinases. Furthermore, the main disadvantages of several typical compounds, including drug resistance, toxicity to non-tumor tissues, and poor aqueous solubility and pharmacokinetic properties, are also discussed, together with strategies to address them. Improved understanding of cancer cell mitosis and natural products may pave the way to drug development for the treatment of cancer.