视网膜脱离巩膜环扎术后眼前节结构的初步观察

目的 观察巩膜环扎术后眼前节结构的变化,探讨术后继发性闭角型青光眼的发病机制。方法 应用超声生物显微镜检查３０例（３３只眼）孔源视网膜脱离患者术前、后３－７天的活体奶前节形态结构,并分别进行定量测定,检查结果经统计学分析两组间配对ｔｓｗｃｗ,以Ｐ〈０．０５作为差异有显著性标准。结果 术前１只眼存在裂隙样睫状体脱离。术后１１只眼（３３．３％）发生睫状体脱离,睫状体增厚水肿;术前睫状体厚度为（８２１．     

老年性痴呆的药物治疗进展

目前尚无治疗老年性痴呆(Alzheimer's disease,AD)的特效方法,但多种药物的应用对于改善认知功能、缓解精神症状、提高患者的生活质量有重要意义.目前老年性痴呆的治疗药物主要涉及神经递质促进剂、脑血液循环改善剂以及脑能量代谢激活剂等.另据报导抗氧化剂、抗炎药、雌激素以及神经生长因子等也对该病有一定疗效.     

The influence of ocular pulsatility on scanning laser Doppler flowmetry.

PURPOSE: To determine the effect of the cardiac cycle on scanning laser Doppler flowmeter measurements of retinal capillary blood flow in rhesus monkeys and humans. METHODS: Multiple scanning laser Doppler flowmetry images of rhesus monkey and human retinal capillary blood flow over a range of heart rates were obtained. Average flow values were determined for the 64 scan lines that compose the two-dimensional flow map. Cutaneous blood flow was measured simultaneously with a laser Doppler flowmeter. The temporal relationships between retinal capillary blood flow, peripheral arterial pulse, and cutaneous blood flow were determined. In addition, human retinal capillary blood flow in a 10 x 10-pixel area during different phases of the cardiac cycle was compared. RESULTS: Regular oscillations in human and rhesus monkey retinal capillary blood flow are evident as alternating bright and dark horizontal bands in scanning laser Doppler flowmetry images. These fluctuations are temporally correlated with cutaneous blood flow. Linear regression of actual vs predicted heart rate based on peaks in retinal capillary flow yielded r = 0.999 in a rhesus monkey and 0.938 in a human. Retinal capillary blood flow in a 10 x 10-pixel area fluctuated as much as 50% depending on the phase of the cardiac cycle. CONCLUSIONS: The alternating bright and dark banding pattern observed in scanning laser Doppler flowmetry scans of retinal capillary blood flow is related to the cardiac pulse. The errors introduced by pulse-related fluctuations in retinal capillary blood flow are significant and must be minimized or corrected for accurate and reproducible measurements of ocular hemodynamics.     

Effects of topical epinephrine on aqueous humor dynamics in the cat

The purpose of this study was to investigate, in cats, the effects of topical epinephrine on aqueous humor dynamics as measured by the non-invasive method of fluorophotometry and by other methods. Measurements were carried out on 12 cats before and after one week of twice daily treatment with 2% epinephrine hydrochloride to one eye. Aqueous flow and outflow facility were determined using fluorophotometry. Uveoscleral outflow was calculated from these results and was evaluated with anterior chamber perfusion of FITC-dextran. Outflow facility also was measured by tonography. Epinephrine-treated eyes, compared with their baseline values, showed a 31% reduction in intraocular pressure (P<0.001), a 23% reduction in aqueous flow (P<0.05), a 60% increase in fluorophotometric outflow facility (P<0.05), and a 43% increase in tonographic outflow facility (P<0.05). Treated eyes, compared with contralateral control eyes, showed a 27% reduction in IOP (P<0.005), a 25% reduction in aqueous flow (P<0.005), a 38% increase in fluorophotometric outflow facility (P<0.05), and a 34% increase in tonographic outflow facility. When evaluated by both fluorophotometry and FITC-dextran tracer methods, epinephrine had no significant effect on uveoscleral outflow. It was concluded that, in cats treated with topical epinephrine twice daily for a week, a reduction in intraocular pressure is induced by an increase in outflow facility and decrease in aqueous flow.     

8—Br—cAMP对人视网膜母细胞瘤HXO—Rb44细胞癌基因的表达效应

目的 研究８－Ｂｒ－ｃＡＭＰ对培养的人视网膜母细胞瘤ＨＸＯ－Ｒｂ４４细胞癌基因表达的效应及其与该细胞生长的关系。方法 ｃ－ｆｏｓ ｍＲＮＡ,Ｎ－ｍｙｃ ｍＲＮＡ及ｐ２１ｒａｓ ｍＲＮＡ均以原位杂交ＲＮＡ斑点印迹技术检测,对繁殖细胞核抗原,ｖ－Ｆｏｓ,Ｎ－Ｍｙｃ和Ｐ＾２１ｒａｓ蛋白表达的免疫反应性则采用免疫组化及收白质斑点印迹技术检测。     

B型超声检查对渗出型老年性黄斑变性的诊断价值

目的:探讨渗出型老年性黄斑变性(Age-related macular degeneration,AMD)的B型超声影象特征。方法:采用NIDEKUS-3300型超声诊断仪,对31例(54只眼)渗出型AMD进行了观察。结果:视网膜色素上皮脱离时,眼内后极部出现膜状弧形回声,光带与眼球壁之间,可见一个梭形透声区;渗出或出血未完全吸收阶段,呈混合性回声;渗出或出血逐渐吸收并为瘢痕组织所替代时,可见实体性小隆起物,强回声,形状似凸透镜或三角形,凸起端指向球后,玻璃体腔面平坦。结论:B型超声在渗出型AMD的诊断、治疗随访观察中具有实用价值。眼科学报1999;15:233-235。     

角膜缘干细胞与结膜移植治疗翼状胬肉

目的:观察角膜缘干细胞与结膜移植治疗翼状胬肉的疗效。方法:采用自体角膜缘干细胞与结膜移植术,对38例43眼初发和复发性翼状胬肉患者进行治疗,术后随访3～30月,平均10月。结果:38例43眼中34例38眼上皮愈合稳定,角膜恢复正常光滑、透明,胬肉无复发。4例5眼失访。结论:自体角膜缘上皮移植为病变区角膜和结膜提供新的干细胞来源,是治疗翼状胬肉的理想方法。眼科学报1999;15:89—90。     

两种LASIK微型角膜刀的临床应用探讨

目的　比较ＫＮ－ ５０００ 型微型角膜刀与Ｍｏｒｉａ 微型角膜刀在ＬＡＳＩＫ 术中制作角膜瓣时的优缺点。方法　选用连续病例５６ 例１０３ 眼,随机分ＫＮ 组和Ｍｏｒｉａ 组,ＫＮ 组２８ 例５３眼,术前近视度数平均为－ ９－２１±７－４１Ｄ,散光≤－５－００Ｄ,Ｍｏｒｉａ 组２８ 人５０ 眼,术前近视度数平均为－１０－４７±３－７３Ｄ,散光≤－２－５０Ｄ,术中分别用ＫＮ－５０００ 微型角膜刀和Ｍｏｒｉａ 微型角膜刀制作角膜瓣。结果　两组病例角膜瓣边缘整齐。基质面：ＫＮ组全部术眼光滑、规则,Ｍｏｒｉａ 组部分术眼有“搓衣板”现象。瓣异常：ＫＮ组游离瓣２ 眼,Ｍｏｒｉａ 组不全瓣５ 眼。ＫＮ组角膜瓣质量优于Ｍｏｒｉａ 组,瓣异常率低于Ｍｏｒｉａ 组。结论　ＫＮ－ ５０００ 微型角膜刀在ＬＡＳＩＫ术中制瓣时性能及安全性、稳定性优于Ｍｏｒｉａ 组微型角膜刀。ＫＮ－ ５０００ 微型角膜刀还具有良好的易学性和进行ＬＡＳＩＫ角膜板层移植的功能。     

碱性成纤维细胞生长因子在人晶体上皮细胞的蛋白检测研究

研究成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ,ｂＦＧＦ）对人晶体上皮细胞（ｈｕｍａｎｌｅｎｓｅｐｉｔｈｅｌｉａｌｃｅｌｓ,ＨＬＥＣｓ）的促增殖作用。方法：采用免疫组织化学检查（ＡＢＣ法）来检测人晶体上皮细胞上ｂＦＧＦ蛋白水平,并用图像分析进行相对定量。结果：定性及定量地证明了人晶体上皮细胞有ｂＦＧＦ蛋白存在。结论：阐明了人晶体上皮细胞本身存在的ｂＦＧＦ以蛋白的形式参与了术后后囊混浊的形成。     

春季结膜炎穹窿结膜的临床病理

证明春季结膜炎在穹窿结膜的临床病理。方法：详细记载球（角膜缘）、睑及穹窿结膜的临床表现;对３８眼（２１例）的穹窿结膜（上穹窿３４眼、下穹窿４眼）进行活检,组织切片ＨＥ染色,光学显微镜下观察病理改变。结果：结膜上皮不同程度增生,上皮下淋巴细胞、浆细胞浸润或胶元纤维增生,３８眼标本中３２眼有嗜酸性白细胞、１眼有嗜碱性白细胞浸润。一极轻病例双眼下穹窿结膜只有很少几个淋巴细胞,其中却散在个别嗜酸性白细胞。结论：春季结膜炎的临床病理改变包括穹窿结膜。