大鼠重组IgE Fc区CH2-3的原核表达及活性研究

目的 研究大鼠IgE的Fc区CH2-3的生物学活性。方法 构建大鼠IgE的Fc区CH2-3的原核表达质粒pBAD／gⅢA／Ch2-3，并转化入TOP10中，阿拉伯糖诱导表达、周质腔抽提、Ni-NTA金属鳌合柱纯化获得大鼠IgE的Fc区CH2-3，细胞及动物水平检测蛋白质生物学活性。结果 原核系统表达出大鼠IgE的Fc区CH2-3，它能够阻断OVA激发的RBL-2H3的脱颗粒反应，阻断被动皮肤实验。结论 大鼠IgE的Fc区CH2-3能够封闭IgE高亲和力受体，阻断过敏反应。     

Adult-onset type II citrullinemia and idiopathic neonatal hepatitis caused by citrin deficiency: involvement of the aspartate glutamate carrier for urea synthesis and maintenance of the urea cycle

Citrin is a mitochondrial aspartate glutamate carrier primarily expressed in the liver, heart, and kidney. We found that adult-onset type II citrullinemia is caused by mutations in the SLC25A13 gene that encodes for citrin. In this report, we describe the frequency of SLC25A13 mutations, the roles of citrin as a member of the urea cycle and as a member of the malate-aspartate shuttle, the relationship between its functions and symptoms of citrin deficiency, and therapeutic issues.     

Novel micro-crosslinked poly(organophosphazenes) with improved mechanical properties and controllable degradation rate as potential biodegradable matrix

As biodegradable materials, linear polyphosphazenes undergo rapid hydrolysis degradation but exhibit poor mechanical properties. Blending with biodegradable polyesters or inorganic particles strengthen their mechanical properties but give rise to slower degradation rate. To balance the mechanical properties and the degradation rate, micro-crosslinked polyphosphazenes were synthesized in this study. Their glass transition temperatures, mechanical properties, and in vitro degradation behavior were investigated. 2-hydroxyethyl methacrylate (HEMA) was firstly attached to the side chain along with glycine ethyl ester to prepare co-substituted poly(organophosphazene) with pendant ethenyl substituents. The co-substituted poly(organophosphazene) was blended with HEMA or acrylic acid (AA) followed by a free radical polymerization to prepare micro-crosslinked poly(organophosphazenes). The resulting crosslinked polymers showed two separate glass transition temperatures depending on the HEMA or AA feed. Incorporation of crosslinking affected the mechanical properties positively. Crosslinked poly(organophosphazenes) showed an approximately 11-17 fold increase in terms of modulus of elasticity when compared to the linear counterpart. In vitro degradation tests indicated that HEMA-crosslinked polymers hydrolyzed at a retarded rate while AA-crosslinked polymers hydrolyzed at a moderate rate compared to linear polymers.     

先天性心脏病儿童白细胞介素2活性及膜表面白细胞介素2受体的研究

目的 研究先天性心脏病（先心病）患儿的免疫功能状态。方法 采用间接免疫荧光检测膜表面白细胞介素2受体（Tac）、植物血凝素刺激后的膜表面白细胞介素2受体（Tac-a),MTT比色法检测白细胞介素2活性（IL－2）。结果 与健康儿童比较,先心病患儿IL－2活性和Tac-a都显著降低（P＜0．01）;与非紫绀型先心病患儿比较,紫绀型先心病患儿IL－2活性显著降低（P＜0．01）。结论 先心病患儿免疫功能缺陷可能是其易患感染性疾病的原因之一,增强免疫治疗对预防先心病患儿感染十分必要。     

药用辅料在制剂中的应用概述

药用辅料是药物制剂的基础材料的重要的组成部分,在制剂型和生产中起着关键作用,它不仅赋予药物一定剂型,并且与提高药物的疗效,降低毒副作用有很大的关系,因此,研究开发,合理应用辅仅可提高药物制剂质量和生产技术水平,而且可取得较大的社会及经济效益。     

改良的颈神经丛阻滞麻醉的临床观察

目的：探讨甲状腺大部切除手术应用改良的颈神经丛阻滞麻醉效果。方法：全部病例均采用一点法深浅颈神经丛阻滞。将全部病例随机分为A、B两组（每组30例）,深丛均为0.25%布比卡因液或1.14%利多卡因和0.14%地卡因混合液,每侧各约5-6ml。A组为与深丛相同浓度的局麻药液,每侧约8-10ml,B组为与A组所用药同等剂量,但其浓度为A组的2/3（即加入1/3容量的生理盐水）,使浅丛容量较A组增加1/3,约为11-13.5ml。结果：两组病人经过顺利,均无并发症。阻滞效果均较好,但A组病人在手术进行到牵拉甲状腺上极时,有12例病人牵拉痛较明显。结论：低浓度高容量法颈丛阻滞,效果可靠、安全、并发症少,是甲状腺大部切除较理想的麻醉方法。     

数字减影泪道造影术的应用

目的：评价数字减影泪道造影术在临床检查泪道阻塞中的应用价值。方法：利用数字减影泪道造影术对82只正常眼及56只溢泪眼进行检查。并对图像进行分析。结果：数字减影泪道造影术能够提供清晰的泪道图像并对泪道阻塞的部位及泪道改变作出准确的判断。结论：数字减影泪道造影术是一种快速、有效和无损伤的检查方法。在泪道阻塞的诊断和治疗中将起重要作用。     

肝硬化患者肾脏血液动力学的研究

目的 研究肝硬化患者肾脏血液动力学的改变。方法 应用多普勒超声测定４２例肝硬化患者肾脏血液动力学指标。并分析这些指标与肝功能及平均动脉压之间的关系。结果 不同阶段的肝硬化患者肾内血管收缩程度有明显的差异。结论 肝硬化早期即有肾血管的收缩,肝功越差肾血管收缩越明显。肾血管的收缩是全身血液动力学改变的一部分。