首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   140927篇
  免费   12403篇
  国内免费   9665篇
耳鼻咽喉   1101篇
儿科学   1724篇
妇产科学   2479篇
基础医学   16090篇
口腔科学   2295篇
临床医学   18277篇
内科学   22420篇
皮肤病学   1561篇
神经病学   7450篇
特种医学   4854篇
外国民族医学   81篇
外科学   14207篇
综合类   23015篇
现状与发展   29篇
一般理论   6篇
预防医学   8957篇
眼科学   3861篇
药学   15078篇
  133篇
中国医学   7331篇
肿瘤学   12046篇
  2024年   409篇
  2023年   2105篇
  2022年   5240篇
  2021年   6645篇
  2020年   4855篇
  2019年   4514篇
  2018年   4566篇
  2017年   4111篇
  2016年   3870篇
  2015年   5824篇
  2014年   7367篇
  2013年   6759篇
  2012年   9842篇
  2011年   10940篇
  2010年   6841篇
  2009年   5434篇
  2008年   7228篇
  2007年   7470篇
  2006年   7462篇
  2005年   7215篇
  2004年   5035篇
  2003年   4766篇
  2002年   4017篇
  2001年   3726篇
  2000年   3836篇
  1999年   3962篇
  1998年   2726篇
  1997年   2372篇
  1996年   1726篇
  1995年   1609篇
  1994年   1388篇
  1993年   889篇
  1992年   1287篇
  1991年   1142篇
  1990年   983篇
  1989年   867篇
  1988年   755篇
  1987年   688篇
  1986年   531篇
  1985年   482篇
  1984年   261篇
  1983年   187篇
  1982年   126篇
  1981年   122篇
  1980年   84篇
  1979年   122篇
  1978年   64篇
  1976年   55篇
  1975年   56篇
  1974年   68篇
排序方式: 共有10000条查询结果,搜索用时 217 毫秒
51.
本研究拟探讨染料木黄酮对前列腺癌细胞LNCaP和CWR22RV1的增殖、迁移和侵袭能力的影响。一、材料与方法1.材料:前列腺癌细胞株LNCaP和CWR22RV1(中国科学院上海生命科学研究院);Genistein(天津科瑞泰科技有限公司);RPMI 1640、胎牛血清(FBS)、胰蛋白酶(美国Gibco公司);E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin,Abcam公司);聚氰基丙烯酸正丁酯(BCA)试剂盒、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)上样缓冲液(×5)及蛋白质印迹法(Western blot)试剂盒(上海市碧云天生物公司);2.实验方法:采用噻唑蓝(MTT)实验、划痕实验和Transwell实验检测Genistein对细胞增殖、迁移及侵袭能力的影响。采用Western blot检测E-cadherin、N-cadherin、Vimentin、CD44和Oct-4的表达水平。  相似文献   
52.

Background

Rosacea is a chronic inflammatory skin condition whose etiology has been linked to mast cells and the antimicrobial peptide cathelicidin LL-37. Individuals with refractory disease have demonstrated clinical benefit with periodic injections of onabotulinum toxin, but the mechanism of action is unknown.

Objectives

To investigate the molecular mechanism by which botulinum toxin improves rosacea lesions.

Methods

Primary human and murine mast cells were pretreated with onabotulinum toxin A or B or control. Mast cell degranulation was evaluated by β-hexosaminidase activity. Expression of botulinum toxin receptor Sv2 was measured by qPCR. The presence of SNAP-25 and VAMP2 was established by immunofluorescence. In vivo rosacea model was established by intradermally injecting LL-37 with or without onabotulinum toxin A pretreatment. Mast cell degranulation was assessed in vivo by histologic counts. Rosacea biomarkers were analyzed by qPCR of mouse skin sections.

Results

Onabotulinum toxin A and B inhibited compound 48/80-induced degranulation of both human and murine mast cells. Expression of Sv2 was established in mouse mast cells. Onabotulinum toxin A and B increased cleaved SNAP-25 and decreased VAMP2 staining in mast cells respectively. In mice, injection of onabotulinum toxin A significantly reduced LL-37-induced skin erythema, mast cell degranulation, and mRNA expression of rosacea biomarkers.

Conclusions

These findings suggest that onabotulinum toxin reduces rosacea-associated skin inflammation by directly inhibiting mast cell degranulation. Periodic applications of onabotulinum toxin may be an effective therapy for refractory rosacea and deserves further study.  相似文献   
53.
54.
55.
综述近年中医药治疗慢性前列腺炎的临床研究,认为本病病因病机之核心在于脾肾亏虚为本,湿热、痰浊、瘀毒为标,病久则伤及脾肾,由实转虚。中医内治法主要以辨证论治、辨病论治或单方验方为主,外治法以中药洗浴、中药灌肠、肛门给药、针灸为主。中医药治疗本病优势明显。应继续完善对中医药作用机制的认识,制订统一的辨证论治及疗效评价标准,针对效果显著的名方开展研究。  相似文献   
56.
Two new bisamides, aglaiamides A (1) and B (2), along with three known ones (35), were isolated from the leaves of Aglaia perviridis. Their structures were established on the basis of detailed spectroscopic analyses.  相似文献   
57.
Capillary malformation–arteriovenous malformation syndrome (CM‐AVM) is an autosomal dominant disorder caused by RASA1 mutations. The prevalence and phenotypic spectrum are unknown. Evaluation of patients with multiple CMs is challenging because associated AVMs can be life threatening. The objective of this study was to describe the clinical characteristics of children presenting with features of CM‐AVM to an academic pediatric dermatology practice. After institutional review board approval was received, a retrospective chart review was performed of patients presenting between 2009 and 2012 with features of CM‐AVM. We report nine cases. Presenting symptoms ranged from extensive vascular stains and cardiac failure to CMs noted incidentally during routine skin examination. All demonstrated multiple CMs, two had Parkes Weber syndrome, and two had multiple infantile hemangiomas. Seven patients had family histories of multiple CMs; three had family histories of large, atypical CMs. Six had personal or family histories of AVMs. Genetic evaluation was recommended for all and was pursued by six families; four RASA1 mutations were identified, including one de novo. Consultations with neurology, cardiology, and orthopedics were recommended. Most patients (89%) have not required treatment to date. CM‐AVM is an underrecognized condition with a wide clinical spectrum that often presents in childhood. Further evaluation may be indicated in patients with multiple CMs. This study is limited by its small and retrospective nature.  相似文献   
58.
59.
<正>随着多媒体应用的普及,解剖学实验教学中也已把多媒体教学作为重要的教学手段。然而,以电脑加投影仪为代表的传统多媒体设备,由于其自身的缺陷,难以完全取代黑板教学,所以多媒体和黑板在教学中最好结合使用以取长补短~([1])。尽管如此,在两者的结合教学中还是存在一些问题。比如,要使投影仪显示效果清晰,需要教室光线黑暗一些,而黑板教学又要求教室光线比较明亮;电子教鞭一晃而过的指示方法让学生感觉不够  相似文献   
60.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号