基于生信学数据挖掘和实验验证阿魏酸介导间充质干细胞外泌体调控炎症miRNA表达情况

目的 基于生信分析筛选出人脐血间充质干细胞来源的外泌体(humanumbilicalcordbloodmesenchymalstem cells-derived exosomes,hUCBMSC-Exos)中可用于调控炎症过程的微小RNA(microRNA,miRNA)。在此基础上,研究阿魏酸(ferulicacid,FA)对hUCBMSC-Exos中与炎症调控相关miRNA的影响。方法 从现有数据库中分别筛选hUCBMSC-Exos中的miRNAs和与调控炎症相关的miRNAs,取2组miRNAs的交集,从而获得hUCBMSC-Exos中与调控炎症相关的候选miRNA分子组。取足月健康新生儿脐带血,将分离所得的单个核细胞置于DMEM/F12培养基培养至P4代细胞,进行成骨、成软骨和成脂诱导分化鉴定。实验分为2个部分来验证FA对hUCBMSC-Exos及hUCBMSCs分泌相关炎症因子的作用：(1)将鉴定成功的hUCBMSC-Exos随机分为实验组及对照组,实验组用2 mg·L^-1 FA干预,对照组用等体积PBS液处理,培养24 h。采用超速离心法结合超滤法提取h...     

升降散临床应用研究进展

升降散为中医升降气机、开郁透热的著名方剂。临床上,该方或其加味方,或与西医药联合使用,用于包括呼吸、消化、循环、泌尿、神经、内分泌、皮肤等在内的多科及多种疾病的治疗,均取得一定疗效。现基于循证的角度,对近十年来关于升降散临床运用方面的研究进行系统梳理和评析,为该方的临床疗效评价及合理运用提供参考。     

腹腔镜在未婚女性巨大卵巢囊肿中的应用

目的 探讨未婚女性巨大卵巢囊肿行腹腔镜手术的方法及其安全性、依从性..方法对21例未婚巨大卵巢囊肿患者行腹腔镜辅助微小切口治疗,镜下行囊肿穿刺抽吸囊液,经2～3 cm Trocar切口将囊肿拉出腹壁外剥除,并行卵巢成形术送回腹腔.结果21例手术均在腹腔镜辅助微小切口下顺利完成,术后病理均为良性,平均手术时间(56±13...     

高校突发事件案例研究——以小白自杀未遂为例

结合高校突发事件的定义、类型,采用事件过程描述的方式,以高校辅导员的视角对突发事件的处理进行详细介绍,重点突出应急操作及事后处理,并反思应对突发事件过程中高校辅导员的重要职责。     

不同年代首次住院心境障碍患者药物应用状况分析

目的 探讨不同年代首次住院心境障碍患者药物应用状况.方法 将1989年度、1999年度、2009年度在我院首次住院的心境障碍患者分别设为A组(77例)、B组(136例)、C组(136例),统计3组患者的药物应用状况.结果 3组新型抗精神病药物、抗抑郁剂、情绪稳定剂及苯二氮艹卓类药物应用频率比较差异均有显著性(P＜0.05或0.01);新型抗精神病药物、新型抗抑郁剂、苯二氮艹卓类药物及情绪稳定剂丙戊酸钠应用频率显著升高.结论 首次住院心境障碍患者新型抗精神病药、新型抗抑郁剂及苯二氮艹卓类药物应用频率有明显增加趋势,而情绪稳定剂中单用丙戊酸钠及丙戊酸钠联合碳酸锂治疗明显增加,临床用药状况趋向合理化.     

脐血源间充质干细胞治疗肌萎缩侧索硬化的临床研究

目的对脐血间充质干细胞（uMscs）治疗肌萎缩侧索硬化进行研究。方法将脐血间充质干细胞用腰穿的方法移植人肌萎缩侧索硬化患者的脑脊液中,术后给予鼠神经生长因子,比较患者治疗前后的变化。结果采用Norris评分量表,表明患者治疗前后的病情有显著改善,差异具有统计学意义（P〈0．05）。结论脐血间充质干细胞对治疗肌萎缩侧索硬化有一定的临床效果。     

Peptide p3 may play a neuroprotective role in the brain

Alzheimer's disease (AD) is an age-related neurodegenerative disease. The amyloid-β (Aβ) peptide is considered a major etiological factor in the development of AD. BACE1-deficient mice and forebrain-specific conditional presenilin1 and presenilin2 double knockout mice (presenilins cDKO mice) both lack Aβ, but exhibit completely different phenotypes. The peptide p3 may play a neuroprotective role. A lack of peptide p3 could trigger an inflammatory response in the brain of presenilins cDKO mice.     

中西医结合治疗放射性直肠炎疗效观察

目的探讨中西医结合治疗放射性直肠炎的经验。方法对320例经放射治疗后出现放射性直肠炎的患者,随机分为两组,中西医结合组和西医组,治疗10d、20d后观察两组疗效及直肠镜检情况。结果中西医结合组对患者症状改善及增进食欲、改善睡眠及精神状况明显优于西医组（P＜0.05）,有显著性差异。结论采用中西医结合药物口服加灌肠治疗放射性直肠炎疗效显著,而且安全可靠、无毒副作用,具有广泛的实用性。     

乳酸菌阴道胶囊联合达克宁栓治疗妊娠期外阴阴道假丝酵母菌病的疗效对照研究

目的：探讨乳酸菌阴道胶囊联合达克宁栓治疗妊娠期外阴阴道假丝酵母菌病的疗效.方法：选择68例妊娠期外阴阴道假丝酵母菌病患者,随机分为联合组和对照组.两组患者均予以达克宁栓放入阴道置于阴道后穹隆处,每晚1枚,连用3d.联合组加用乳酸菌阴道胶囊放入阴道置于阴道后穹隆处,早上2枚,连用5d.对照组除不使用乳酸菌阴道胶囊外余治疗均同联合组.观察两组患者治疗结束后1周的临床疗效,并随访治疗后3个月的复发率.结果：联合组患者治疗后的临床总有效率明显高于对照组（χ2=6.93,P〈0.01）.对照组与联合组治疗后3个月内分别复发13例和4例.联合组患者治疗后3个月内复发率明显低于对照组（χ2=6.35,P〈0.05）.结论：乳酸菌阴道胶囊联合达克宁栓治疗妊娠期外阴阴道假丝酵母菌病较确切,能明显降低复发率,具有治疗和预防外阴阴道假丝酵母菌病复发的作用.     

Genome-wide detection of natural selection in African Americans pre- and post-admixture

It is particularly meaningful to investigate natural selection in African Americans (AfA) due to the high mortality their African ancestry has experienced in history. In this study, we examined 491,526 autosomal single nucleotide polymorphisms (SNPs) genotyped in 5210 individuals and conducted a genome-wide search for selection signals in 1890 AfA. Several genomic regions showing an excess of African or European ancestry, which were considered the footprints of selection since population admixture, were detected based on a commonly used approach. However, we also developed a new strategy to detect natural selection both pre- and post-admixture by reconstructing an ancestral African population (AAF) from inferred African components of ancestry in AfA and comparing it with indigenous African populations (IAF). Interestingly, many selection-candidate genes identified by the new approach were associated with AfA-specific high-risk diseases such as prostate cancer and hypertension, suggesting an important role these disease-related genes might have played in adapting to a new environment. CD36 and HBB, whose mutations confer a degree of protection against malaria, were also located in the highly differentiated regions between AAF and IAF. Further analysis showed that the frequencies of alleles protecting against malaria in AAF were lower than those in IAF, which is consistent with the relaxed selection pressure of malaria in the New World. There is no overlap between the top candidate genes detected by the two approaches, indicating the different environmental pressures AfA experienced pre- and post-population admixture. We suggest that the new approach is reasonably powerful and can also be applied to other admixed populations such as Latinos and Uyghurs.