全文获取类型
收费全文 | 7226篇 |
免费 | 515篇 |
国内免费 | 72篇 |
专业分类
耳鼻咽喉 | 51篇 |
儿科学 | 180篇 |
妇产科学 | 214篇 |
基础医学 | 1001篇 |
口腔科学 | 102篇 |
临床医学 | 641篇 |
内科学 | 1856篇 |
皮肤病学 | 179篇 |
神经病学 | 889篇 |
特种医学 | 274篇 |
外科学 | 612篇 |
综合类 | 11篇 |
预防医学 | 452篇 |
眼科学 | 52篇 |
药学 | 544篇 |
中国医学 | 15篇 |
肿瘤学 | 740篇 |
出版年
2024年 | 15篇 |
2023年 | 125篇 |
2022年 | 260篇 |
2021年 | 374篇 |
2020年 | 240篇 |
2019年 | 304篇 |
2018年 | 312篇 |
2017年 | 255篇 |
2016年 | 293篇 |
2015年 | 330篇 |
2014年 | 395篇 |
2013年 | 477篇 |
2012年 | 711篇 |
2011年 | 694篇 |
2010年 | 380篇 |
2009年 | 285篇 |
2008年 | 417篇 |
2007年 | 381篇 |
2006年 | 357篇 |
2005年 | 289篇 |
2004年 | 230篇 |
2003年 | 203篇 |
2002年 | 159篇 |
2001年 | 30篇 |
2000年 | 28篇 |
1999年 | 24篇 |
1998年 | 34篇 |
1997年 | 15篇 |
1996年 | 15篇 |
1995年 | 11篇 |
1994年 | 5篇 |
1993年 | 8篇 |
1992年 | 12篇 |
1991年 | 13篇 |
1990年 | 14篇 |
1989年 | 16篇 |
1988年 | 9篇 |
1987年 | 5篇 |
1986年 | 11篇 |
1985年 | 9篇 |
1984年 | 9篇 |
1983年 | 6篇 |
1979年 | 8篇 |
1977年 | 6篇 |
1975年 | 3篇 |
1973年 | 3篇 |
1970年 | 4篇 |
1969年 | 6篇 |
1968年 | 3篇 |
1960年 | 2篇 |
排序方式: 共有7813条查询结果,搜索用时 15 毫秒
41.
Alexey D. Sereda Mikhail E. Vlasov Galina S. Koltsova Sergey Y. Morgunov Dmitry A. Kudrjashov Irina P. Sindryakova Olga L. Kolbasova Valentina M. Lyska Andrei Y. Koltsov Sergey P. Zhivoderov Elena Y. Pivova Vladimir M. Baluishev Andrey E. Gogin Denis V. Kolbasov 《Viruses》2022,14(8)
The African swine fever virus (ASFV) is the cause of a recent pandemic that is threatening the global pig industry. The virus infects domestic and wild pigs and manifests with a variety of clinical symptoms, depending on the strain. No commercial vaccine is currently available to protect animals from this virus, but some attenuated and recombinant live vaccine candidates might be effective against the disease. This article describes the immunobiological characteristics of one such candidate—the laboratory-attenuated ASFV strain, Katanga-350—which belongs to genotype I. In this study, we assessed clinical signs and post-mortem changes, the levels of viremia and the presence of viral DNA caused by injection of ASF virus strains Katanga-350, Lisbon-57, and Stavropol 08/01. Intramuscular injection of this strain protected 80% of pigs from a virulent strain of the same genotype and seroimmunotype (Lisbon-57). At least 50% of the surviving pigs received protection from subsequent intramuscular infection with a heterologous (genotype II, seroimmunotype VIII) virulent strain (Stavropol 08/01). Virus-specific antibodies were detectable in serum and saliva samples between 8–78 days after the first inoculation of the Katanga-350 strain (the observational period). The results suggested that this strain could serve as a basis for the development of a recombinant vaccine against ASF viruses belonging to seroimmunotype I. 相似文献
42.
Valentina Cariello Patrizia Lombardo Luisa Castelli Carmela Brillantino Carmela De Fusco Antonio Rossi Rocco Minelli Giuseppe Paviglianiti Roberto Grassi Eugenio Rossi 《Radiology Case Reports》2022,17(8):2747
Langerhans cell histiocytosis (LCH) is a myeloid neoplasm characterized by a clonal proliferation of CD1a+/CD207+ dendritic cells. Although individuals of any age can be affected, the disease is most common in infants younger than 5 years of age, especially males. A wide range of manifestations, from asymptomatic to aggressive, have been described, along with multiorgan involvement. Even though the majority of bone lesions are observed, skin, lymph nodes, brain and lungs can also be involved. The involvement of hematopoietic system, including bone marrow, liver and spleen, is less frequent yet associated with worse prognosis, due to a worse treatment response. Diagnosis of LCH is based on the integration of clinical, laboratory, and radiological data; however, only histopathological examination might confirm it. As far as the spleen involvement is concerned, according to literature, it has been reported in about 15% patients with multisystem involvement, nonetheless only a few cases show parenchymal lesions. The present study reports the case of an infant with LCH with multisystem involvement, including bone, skin, liver, and spleen, with evidence of parenchymal lesions. 相似文献
43.
Matic Mihevc rt Zavrnik Majda Mori Lukan
i
Tina Virti
Valentina Prevolnik Rupel Marija Petek ter Zalika Klemenc Keti Antonija Poplas Susi
《Slovenian Journal of Public Health》2022,61(4):216
IntroductionArterial hypertension (AH) and type 2 diabetes (T2D) represent a significant burden for the public health system, with an exceptionally high prevalence in patients aged ≥65 years. This study aims to test the acceptability, clinical effectiveness, and cost-effectiveness of telemonitoring in elderly patients with AH and T2D at the primary care level.MethodsA m ulti-centre, prospective, randomized, controlled t rial w ill be conducted. Patients a ged ≥ 65 y ears with AH and T2D will be randomized in a 1:1 proportion to a mHealth intervention or standard care group. Patients in the intervention group will measure their blood pressure (BP) twice weekly and blood glucose (BG) once monthly. The readings will be synchronously transmitted via a mobile application to the telemonitoring platform, where they will be reviewed by a general practitioner who will indicate changes in measurement regimen or carry out a teleconsultation. The primary endpoint will be a change in systolic BP (SBP) and glycated haemoglobin (HbA1c) relative to standard care up to 12 months after inclusion. Secondary endpoints will be a change in other observed clinical variables, quality-of-life indexes, and costs.Expected resultsTelemonitoring will be an acceptable method of care associated with significant reductions in SBP and HbA1c levels and an increase in quality-of-life indexes in the intervention group. However, the cost-effectiveness threshold (incremental cost-effectiveness ratio below €25,000/quality-adjusted life year) might not be reached.ConclusionThis study will provide new evidence for scaling up telemonitoring network at the primary care level and modifying telemonitoring protocols to achieve the best clinical and cost-effective outcomes. 相似文献
44.
This paper reviews the state-of-the-art technologies, characterizations, materials (precursors and encapsulants), and challenges concerning multicolor and white light-emitting diodes (LEDs) based on carbon dots (CDs) as color converters. Herein, CDs are exploited to achieve emission in LEDs at wavelengths longer than the pump wavelength. White LEDs are typically obtained by pumping broad band visible-emitting CDs by an UV LED, or yellow–green-emitting CDs by a blue LED. The most important methods used to produce CDs, top-down and bottom-up, are described in detail, together with the process that allows one to embed the synthetized CDs on the surface of the pumping LEDs. Experimental results show that CDs are very promising ecofriendly candidates with the potential to replace phosphors in traditional color conversion LEDs. The future for these devices is bright, but several goals must still be achieved to reach full maturity. 相似文献
45.
Susanna Esposito Ilaria Testa Elena Mariotti Zani Daniela Cunico Lisa Torelli Roberto Grandinetti Valentina Fainardi Giovanna Pisi Nicola Principi 《Nutrients》2022,14(15)
In the last 20 years, gut microbiota in patients with cystic fibrosis (CF) has become an object of interest. It was shown that these patients had gut dysbiosis and this could explain not only the intestinal manifestations of the disease but also part of those involving the respiratory tract. The acquisition of previously unknown information about the importance of some bacteria, i.e., those partially or totally disappeared in the gut of CF patients, in the regulation of the activity and function of the gut and the lung was the base to suggest the use of probiotics in CF patients. The main aim of this paper is to discuss the biological basis for probiotic administration to CF patients and which results could be expected. Literature analysis showed that CF intestinal dysbiosis depends on the same genetic mutations that condition the clinical picture of the diseases and is aggravated by a series of therapeutic interventions, such as dietary modifications, the use of antibiotics, and the administration of antacids. All this translates into a significant worsening of the structure and function of organs, including the lung and intestine, already deeply penalized by the genetic alterations of CF. Probiotics can intervene on dysbiosis, reducing the negative effects derived from it. However, the available data cannot be considered sufficient to indicate that these bacteria are essential elements of CF therapy. Further studies that take into account the still unsolved aspects on how to use probiotics are absolutely necessary. 相似文献
46.
Possible risk for cancer among children born following assisted reproductive technology in Israel 下载免费PDF全文
47.
Domenica Giannandrea Natalia Platonova Michela Colombo Mara Mazzola Valentina Citro Raffaella Adami Filippo Maltoni Silvia Ancona Vincenza Dolo Ilaria Giusti Andrea Basile Anna Pistocchi Laura Cantone Valentina Bollati Lavinia Casati Elisabetta Calzavara Mauro Turrini Elena Lesma Raffaella Chiaramonte 《Haematologica》2022,107(9):2183
Multiple myeloma (MM) is an incurable hematologic neoplasm, whose poor prognosis is deeply affected by the propensity of tumor cells to localize in the bone marrow (BM) and induce the protumorigenic activity of normal BM cells, leading to events associated with tumor progression, including tumor angiogenesis, osteoclastogenesis, and the spread of osteolytic bone lesions. The interplay between MM cells and the BM niche does not only rely on direct cell-cell interaction, but a crucial role is also played by MM-derived extracellular vesicles (MM-EV). Here, we demonstrated that the oncogenic NOTCH receptors are part of MM-EV cargo and play a key role in EV protumorigenic ability. We used in vitro and in vivo models to investigate the role of EV-derived NOTCH2 in stimulating the protumorigenic behavior of endothelial cells and osteoclast progenitors. Importantly, MM-EV can transfer NOTCH2 between distant cells and increase NOTCH signaling in target cells. MM-EV stimulation increases endothelial cell angiogenic ability and osteoclast differentiation in a NOTCH2-dependent way. Indeed, interfering with NOTCH2 expression in MM cells may decrease the amount of NOTCH2 also in MM-EV and affect their angiogenic and osteoclastogenic potential. Finally, we demonstrated that the pharmacologic blockade of NOTCH activation by γ-secretase inhibitors may hamper the biological effect of EV derived by MM cell lines and by the BM of MM patients. These results provide the first evidence that targeting the NOTCH pathway may be a valid therapeutic strategy to hamper the protumorigenic role of EV in MM as well as other tumors. 相似文献
48.
Fedor M. Zurabov Ekaterina A. Chernevskaya Natalia V. Beloborodova Alexander Yu. Zurabov Marina V. Petrova Mikhail Ya. Yadgarov Valentina M. Popova Oleg E. Fatuev Vladislav E. Zakharchenko Marina M. Gurkova Ekaterina A. Sorokina Egor A. Glazunov Tatiana A. Kochetova Victoria V. Uskevich Artem N. Kuzovlev Andrey V. Grechko 《Viruses》2022,14(12)
Increasing evidence suggests that gut dysbiosis is associated with coronavirus disease 2019 (COVID-19) infection and may persist long after disease resolution. The excessive use of antimicrobials in patients with COVID-19 can lead to additional destruction of the microbiota, as well as to the growth and spread of antimicrobial resistance. The problem of bacterial resistance to antibiotics encourages the search for alternative methods of limiting bacterial growth and restoring the normal balance of the microbiota in the human body. Bacteriophages are promising candidates as potential regulators of the microbiota. In the present study, two complex phage cocktails targeting multiple bacterial species were used in the rehabilitation of thirty patients after COVID-19, and the effectiveness of the bacteriophages against the clinical strain of Klebsiella pneumoniae was evaluated for the first time using real-time visualization on a 3D Cell Explorer microscope. Application of phage cocktails for two weeks showed safety and the absence of adverse effects. An almost threefold statistically significant decrease in the anaerobic imbalance ratio, together with an erythrocyte sedimentation rate (ESR), was detected. This work will serve as a starting point for a broader and more detailed study of the use of phages and their effects on the microbiome. 相似文献
49.
Ludovica Natali MSc Valentina Meregalli MSc Katie Rowlands BSc Jerome Di Pietro BA Janet Treasure MD Enrico Collantoni MD Paolo Meneguzzo MD Elena Tenconi PhD Angela Favaro MD Francesca Fontana MD Enrico Ceccato DClinPsych Alessandra Sala MD Lucia Valmaggia PhD Valentina Cardi PhD 《The International journal of eating disorders》2024,57(3):703-715
50.
Anna Onnis Emanuele Andreano Chiara Cassioli Francesca Finetti Chiara Della Bella Oskar Staufer Elisa Pantano Valentina Abbiento Giuseppe Marotta Mario Milco DElios Rino Rappuoli Cosima T. Baldari 《The Journal of experimental medicine》2023,220(2)
CTL-mediated killing of virally infected or malignant cells is orchestrated at the immune synapse (IS). We hypothesized that SARS-CoV-2 may target lytic IS assembly to escape elimination. We show that human CD8+ T cells upregulate the expression of ACE2, the Spike receptor, during differentiation to CTLs. CTL preincubation with the Wuhan or Omicron Spike variants inhibits IS assembly and function, as shown by defective synaptic accumulation of TCRs and tyrosine phosphoproteins as well as defective centrosome and lytic granule polarization to the IS, resulting in impaired target cell killing and cytokine production. These defects were reversed by anti-Spike antibodies interfering with ACE2 binding and reproduced by ACE2 engagement by angiotensin II or anti-ACE2 antibodies, but not by the ACE2 product Ang (1-7). IS defects were also observed ex vivo in CTLs from COVID-19 patients. These results highlight a new strategy of immune evasion by SARS-CoV-2 based on the Spike-dependent, ACE2-mediated targeting of the lytic IS to prevent elimination of infected cells. 相似文献