全文获取类型
收费全文 | 35345篇 |
免费 | 2878篇 |
国内免费 | 414篇 |
专业分类
耳鼻咽喉 | 372篇 |
儿科学 | 1039篇 |
妇产科学 | 639篇 |
基础医学 | 5088篇 |
口腔科学 | 529篇 |
临床医学 | 3361篇 |
内科学 | 7598篇 |
皮肤病学 | 777篇 |
神经病学 | 2657篇 |
特种医学 | 1357篇 |
外国民族医学 | 17篇 |
外科学 | 4373篇 |
综合类 | 1287篇 |
现状与发展 | 1篇 |
一般理论 | 2篇 |
预防医学 | 2823篇 |
眼科学 | 688篇 |
药学 | 2686篇 |
6篇 | |
中国医学 | 638篇 |
肿瘤学 | 2699篇 |
出版年
2023年 | 290篇 |
2022年 | 709篇 |
2021年 | 1174篇 |
2020年 | 725篇 |
2019年 | 891篇 |
2018年 | 1082篇 |
2017年 | 792篇 |
2016年 | 784篇 |
2015年 | 957篇 |
2014年 | 1219篇 |
2013年 | 1567篇 |
2012年 | 2282篇 |
2011年 | 2267篇 |
2010年 | 1405篇 |
2009年 | 1164篇 |
2008年 | 1679篇 |
2007年 | 1708篇 |
2006年 | 1659篇 |
2005年 | 1540篇 |
2004年 | 1362篇 |
2003年 | 1307篇 |
2002年 | 1236篇 |
2001年 | 1119篇 |
2000年 | 1110篇 |
1999年 | 993篇 |
1998年 | 348篇 |
1997年 | 309篇 |
1996年 | 267篇 |
1995年 | 213篇 |
1994年 | 210篇 |
1993年 | 174篇 |
1992年 | 520篇 |
1991年 | 499篇 |
1990年 | 479篇 |
1989年 | 435篇 |
1988年 | 454篇 |
1987年 | 384篇 |
1986年 | 378篇 |
1985年 | 369篇 |
1984年 | 258篇 |
1983年 | 189篇 |
1982年 | 121篇 |
1981年 | 106篇 |
1979年 | 216篇 |
1978年 | 111篇 |
1977年 | 125篇 |
1976年 | 117篇 |
1974年 | 134篇 |
1972年 | 129篇 |
1969年 | 104篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
171.
172.
Jegdish P. Babu Ronald F. Schell Jack L. Le Frock 《Journal of clinical microbiology》1978,8(3):288-292
Several investigators have evaluated clinically a variety of commercially available blood culture media. No agreement has been reached as to which of these media is optimal for detection of bacteremia. The purpose of this study was to determine the rate of recovery of microorganisms from various blood culture media. A total of 23 blood culture media were inoculated with 7 to 15 microorganisms per bottle in the presence or absence of an erythrocyte-serum mixture. The results demonstrated that blood culture media differed in their ability to support the growth of microorganisms. At 4 days after inoculation, only 10 of the 23 blood culture media supported the growth of 91% (10 of the 11) or more of the test microorganisms. The recovery rate of microorganisms depended not only upon the type of medium but also upon the manufacturer of the type of blood culture medium. The addition of an erythrocyte-serum mixture to the blood culture media did not influence the difference in the recovery rate of microorganisms among media and the same type of medium prepared by different manufacturers. The majority (15 of the 23) of the blood culture media supplemented with the erythrocyte-serum mixture failed to support the growth of 91% or more of the test microorganisms at 4 days after inoculation. These results have demonstrated that blood culture media need to be improved. Better quality control measures should also be implemented to evaluate commercial blood culture media. 相似文献
173.
P.-Y. Le Pennec F. Noizat-Pirenne M.T. Klein D. Fraval J.M. Lascaux Ph. Rouger 《Transfusion Clinique et Biologique》1996,3(6):401-404
In this paper we chose to emphasize three aspects of our work. First we underlined that “low grade and high grade” D weak red blood cells studied at the DNA level could, when monoclonal antibodies were used, give patterns of positive and negative reactions like partial RH1(D) cells. Secondly, we showed the importance of the technical conditions of the study which are essential for establishing a pattern of reactivity defining an epitope. It appears that the use of papain treated cells at room temperature can be misleading for the definition of epitope especially with IgM antibodies. Lastly we pointed out the interest of Rh variant cells, defined at the gene level, to study the expression of RH1(D) epitopes on the external part of the membrane. 相似文献
174.
Fifty-eight patients with well-documented history of seasonal rhinoconjunctivitis caused by grass pollens were allocated randomly on a double-blind basis to receive either sublingual therapy with a solution of purified, standardized allergen preparation (Stallergenes) or a matched placebo for 17 weeks. The assessment of the effect of oral immunotherapy, done with drops of five-grass allergen extract, was on the clinical symptoms and on the medication score of the authorized rescue treatments. The actively treated patients had significantly (P<0.05 to P<0.01) fewer symptoms of rhinitis (sneezing and rhinorrhea) and of conjunctivitis (redness and tears) during the pollen season than the placebo group. Consumption of nasal solution of sodium cromoglycate and of betamethasone and dexchlorpheniramine was significantly less in the desensitized group (P<0.01). Side-effects were negligible. This study concludes that perlingual immunotherapy with grass pollen extract in grass-pollen-sensitive seasonal hay fever and conjunctivitis patients is effective, easy to perform, inexpensive, and safe. 相似文献
175.
Sylvestre Marchaux Paul Fornes Stphanie Petit Catherine Poisson Didier Thevenin Thierry Le Tourneau Philippe Asseman Patrick Bruneval Pierre-Vladimir Ennezat 《Cardiovascular pathology》2008,17(4):241-243
Myocardial dysfunction without coronary involvement may occur in acute cerebral diseases. The inverted Takotsubo pattern has been recently recognized as a novel heart neurologic stress-related syndrome. We report on the case of a 40-year-old woman presenting with massive subarachnoid hemorrhage and brain death. Echocardiography revealed an extensive left ventricular circumferential akinesis except at the apex. Histologic analysis of the heart confirmed the absence of myocardial infarction and revealed only sparse foci of myocyte necrosis with contraction bands in the akinetic areas. 相似文献
176.
Missense FGFR3 mutations create cysteine residues in thanatophoric dwarfism type I (TD1) 总被引:10,自引:1,他引:10
Rousseau F; el Ghouzzi V; Delezoide AL; Legeai-Mallet L; Le Merrer M; Munnich A; Bonaventure J 《Human molecular genetics》1996,5(4):509-512
Thanatophoric dwarfism (TD) is a sporadic lethal skeletal dysplasia with
micromelic shortening of the limbs, macrocephaly, platyspondyly and reduced
thoracic cavity. In the most common subtype (TD1), femurs are curved, while
in TD2, straight femurs are associated with cloverleaf skull. Mutations in
the fibroblast growth factor receptor 3 (FGFR3) gene were identified in
both subtypes. While TD2 was accounted for by a single recurrent mutation
in the tyrosine kinase 2 domain, TD1 resulted from either stop codon
mutations or missense mutations in the extracellular domain of the gene.
Here, we report the identification of FGFR3 mutations in 25/26 TD cases.
Two novel missense mutations (Y373C and G370C) were detected in 8/26 and
1/26 TD1 cases respectively. Both mutations created cysteine residues in
the juxta extramembrane domain of the receptor. Sixteen cases carried the
previously reported R248C (9/26 cases), S249C (2/26 cases) or stop codon
FGFR3 mutations (5/26 cases). Our results suggest that TD1 is a genetically
homogeneous condition and give additional support to the view that newly
created cysteine residues in the extracellular domain of the protein play a
key role in the severity of the disease.
相似文献
177.
The antibody spectrum in individuals with defect expression of HLA class II and the LFA-1 glycoprotein family genes
下载免费PDF全文
![点击此处可从《Clinical and experimental immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
C I Smith C Bremard-Oury F Le Deist C Griscelli P Aucouturier G M?ller L Hammarstr?m J L Preud'homme R S Weening 《Clinical and experimental immunology》1988,74(3):449-453
HLA class II antigens and the LFA-1 (lymphocyte function-associated type 1) glycoprotein family are cell surface structures of central importance in many lymphocyte reactions. Specific antibodies are normally restricted to particular IgG subclasses. In order to study the mechanism of isotype restriction we have analysed specific IgG1, IgG2, IgG3 and IgG4 antibodies directed against a number of different protein and polysaccharide antigens in individuals with HLA class II or LFA-1 deficiency. HLA class II deficiency resulted in decreased total IgG2, IgG4 and IgA in 4/4 patients, whereas total IgM and IgG were low in 2/4. In HLA class II deficiency the levels of specific antibodies directed against protein as well as polysaccharide antigens were frequently low. Virtually normal total as well as specific antibody levels were found in individuals with both severe and moderate forms of LFA-1 deficiency. There was no clear evidence for an abnormal subclass pattern of specific antibodies with a shift from one subclass to an isotype which is normally not used, in any of the patient groups investigated. 相似文献
178.
179.
Pierre-Andr Bcherel Liliane Le Goff Sandra Ktorza Fateh Ouaaz Jean-Michel Mencia-Huerta Bernard Dugas Patrice Debr M. Djavad Mossalayi Michel Arock 《European journal of immunology》1995,25(10):2992-2995
Human keratinocytes (HK) generate nitric oxide (NO) and proinflammatory mediators following activation with either IgE/anti-IgE immune complexes or a combination of lipopolysaccharide (LPS) and interferon-γ (IFN-γ). Recently, interleukin-10 (IL-10) has been shown to down-regulate various inflammatory responses and to be secreted by lymphocytes and dendritic cells during skin inflammatory reactions. We show here that IL-10 down-regulates the production of tumor necrosis factor (TNF)-α and IL-6 by activated HK. Also, induction of inducible nitric oxide synthase (iNOS) expression in HK by IgE/anti-IgE or LPS/IFN-γ is significantly reduced by the addition of IL-10. This effect is dose dependent and correlates with reduction of iNOS mRNA production and enzyme level. Therefore, IL-10 down-regulates NO-mediated HK inflammatory responses and may thus participate in the regulation of the skin immune network. 相似文献
180.
Cellular receptors play an important role in viral pathogenesis. Until now little was known on echovirus (EV) receptor. Using detergent-treated KB cell extracts as immunogen, a mouse monoclonal antibody (Mab 143) was produced that selectively blocks the attachment of EV-11 to KB and other susceptible cells. By immunoblotting, Mab 143 detected a 44,000 protein on susceptible cell lines but not on cell lines from nonprimate origin. The receptor protein complex, purified from KB cell membranes by immunoaffinity using Mab 143 as ligand, was shown to contain a single glycoprotein with apparent molecular weight of 44,000 (gp44). The role of gp44 in the attachment of EV-11 onto KB cells was demonstrated by the ability (i) of affinity-purified gp44 to reduce the infectivity of EV-11 and (ii) of rabbit polyclonal antisera raised against gp44 to protect cells from the replication of various EV, as did Mab 143. 相似文献