全文获取类型
收费全文 | 8316篇 |
免费 | 397篇 |
国内免费 | 38篇 |
专业分类
耳鼻咽喉 | 52篇 |
儿科学 | 197篇 |
妇产科学 | 60篇 |
基础医学 | 986篇 |
口腔科学 | 175篇 |
临床医学 | 512篇 |
内科学 | 1945篇 |
皮肤病学 | 121篇 |
神经病学 | 404篇 |
特种医学 | 413篇 |
外科学 | 1846篇 |
综合类 | 98篇 |
预防医学 | 217篇 |
眼科学 | 106篇 |
药学 | 788篇 |
中国医学 | 5篇 |
肿瘤学 | 826篇 |
出版年
2023年 | 50篇 |
2022年 | 73篇 |
2021年 | 119篇 |
2020年 | 78篇 |
2019年 | 111篇 |
2018年 | 135篇 |
2017年 | 98篇 |
2016年 | 123篇 |
2015年 | 133篇 |
2014年 | 172篇 |
2013年 | 237篇 |
2012年 | 328篇 |
2011年 | 387篇 |
2010年 | 246篇 |
2009年 | 205篇 |
2008年 | 357篇 |
2007年 | 346篇 |
2006年 | 328篇 |
2005年 | 354篇 |
2004年 | 302篇 |
2003年 | 292篇 |
2002年 | 341篇 |
2001年 | 295篇 |
2000年 | 334篇 |
1999年 | 308篇 |
1998年 | 148篇 |
1997年 | 116篇 |
1996年 | 124篇 |
1995年 | 98篇 |
1994年 | 84篇 |
1993年 | 71篇 |
1992年 | 241篇 |
1991年 | 200篇 |
1990年 | 216篇 |
1989年 | 185篇 |
1988年 | 190篇 |
1987年 | 188篇 |
1986年 | 151篇 |
1985年 | 179篇 |
1984年 | 97篇 |
1983年 | 70篇 |
1982年 | 48篇 |
1979年 | 85篇 |
1978年 | 47篇 |
1977年 | 44篇 |
1976年 | 33篇 |
1974年 | 40篇 |
1972年 | 36篇 |
1970年 | 32篇 |
1969年 | 42篇 |
排序方式: 共有8751条查询结果,搜索用时 0 毫秒
921.
922.
Normal 123I‐MIBG uptake areas may be associated with hyperinnervation and arrhythmia risk in phenol model rabbit hearts 下载免费PDF全文
923.
Protective action of luminal bile salts in necrotizing acute pancreatitis in mice. 总被引:1,自引:0,他引:1 下载免费PDF全文
G Gomez C M Townsend Jr D W Green S Rajaraman T Uchida G H Greeley Jr R D Soloway J C Thompson 《The Journal of clinical investigation》1990,86(1):323-331
Bile salts in the intestinal lumen act to inhibit the release of cholecystokinin (CCK). Recent studies have shown that CCK may play a permissive role in the development of acute pancreatitis. In this study, the amount of luminal bile salts in female Swiss Webster mice was either decreased by feeding 4% (wt/wt) cholestyramine or increased by feeding 0.5% sodium taurocholate for 1 wk. Plasma levels of CCK were stimulated by cholestyramine and inhibited by taurocholate. Then, acute pancreatitis was induced either by caerulein injections, or by feeding a choline-deficient, ethionine-supplemented (CDE) diet. Feeding of cholestyramine significantly decreased survival from 25% to 0% in the CDE pancreatitis, and increased the magnitude of elevation of serum amylase levels and the extent of pancreatic necrosis in both models of pancreatitis; CCK-receptor blockade with CR-1409 completely abolished the adverse effects of cholestyramine. In contrast, feeding of taurocholate significantly increased survival to 100% and decreased the elevation of serum amylase and pancreatic necrosis; CCK-8 antagonized these actions of taurocholate. Luminal bile salts appear to provide a physiologic protection against necrotizing pancreatitis, at least in part, both by inhibiting the release of CCK and by promoting resistance of the pancreas to CCK excessive stimulation in vivo. 相似文献
924.
PKClambda regulates glucose-induced insulin secretion through modulation of gene expression in pancreatic beta cells 下载免费PDF全文
Hashimoto N Kido Y Uchida T Matsuda T Suzuki K Inoue H Matsumoto M Ogawa W Maeda S Fujihara H Ueta Y Uchiyama Y Akimoto K Ohno S Noda T Kasuga M 《The Journal of clinical investigation》2005,115(1):138-145
Altered regulation of insulin secretion by glucose is characteristic of individuals with type 2 diabetes mellitus, although the mechanisms that underlie this change remain unclear. We have now generated mice that lack the lambda isoform of PKC in pancreatic beta cells (betaPKClambda(-/-) mice) and show that these animals manifest impaired glucose tolerance and hypoinsulinemia. Furthermore, insulin secretion in response to high concentrations of glucose was impaired, whereas the basal rate of insulin release was increased, in islets isolated from betaPKClambda(-/-) mice. Neither the beta cell mass nor the islet insulin content of betaPKClambda(-/-) mice differed from that of control mice, however. The abundance of mRNAs for Glut2 and HNF3beta was reduced in islets of betaPKClambda(-/-) mice, and the expression of genes regulated by HNF3beta was also affected (that of Sur1 and Kir6.2 genes was reduced, whereas that of hexokinase 1 and hexokinase 2 genes was increased). Normalization of HNF3beta expression by infection of islets from betaPKClambda(-/-) mice with an adenoviral vector significantly reversed the defect in glucose-stimulated insulin secretion. These results indicate that PKClambda plays a prominent role in regulation of glucose-induced insulin secretion by modulating the expression of genes important for beta cell function. 相似文献
925.
BACKGROUND: It has not previously been reported that WBC-reduced RBC preparations can cause transfusion-associated GVHD, even in an immunocompetent individual. CASE REPORT: A 74-year-old man suffered a hemorrhage from the mesentery of the transverse colon after a traffic accident. During surgery, he received 10 units of RBCs from 10 donors in a solution containing mannitol, adenine, phosphate, citrate, glucose and NaCl (MAP). MAP RBCs had been stored for 7 to 8 days before use. On the 27th day after surgery, an erythematous, pruritic rash appeared over the face, neck, and trunk, which was associated with low-grade fever and pancytopenia. Transfusion-associated GVHD was strongly suspected and was confirmed by skin biopsy. To determine the origin of lymphocytes causing GVHD, several microsatellite loci were amplified from DNA of the patient's nails and blood and from blood samples of all 10 RBC donors by using PCR. Amplified alleles derived from the patient's blood were identical to those from one of the 10 samples. CONCLUSION: These findings indicate that transfusions of MAP-RBCs can cause transfusion-associated GVHD in an elderly but immunocompetent host. 相似文献
926.
High-affinity autoantibodies specifically eliminate granulocyte-macrophage colony-stimulating factor activity in the lungs of patients with idiopathic pulmonary alveolar proteinosis 总被引:8,自引:0,他引:8 下载免费PDF全文
Uchida K Nakata K Trapnell BC Terakawa T Hamano E Mikami A Matsushita I Seymour JF Oh-Eda M Ishige I Eishi Y Kitamura T Yamada Y Hanaoka K Keicho N 《Blood》2004,103(3):1089-1098
Deficiency of granulocyte-macrophage colony-stimulating factor (GM-CSF) in mice results in pulmonary alveolar proteinosis (PAP) from impaired surfactant catabolism by alveolar macrophages (AMs). Recently, we have shown that neutralizing anti-GM-CSF autoantibodies develop specifically in patients with idiopathic pulmonary alveolar proteinosis (iPAP). Analogous to murine PAP models, it is plausible that the autoantibodies reduce GM-CSF activity, resulting in AM dysfunction and surfactant accumulation. To examine this hypothesis, we estimated the neutralizing activity of the autoantibodies in the lungs of patients and characterized their biologic properties. GM-CSF bioactivity was completely abrogated in the bronchoalveolar lavage fluid (BALF) of patients with iPAP but not in healthy subjects. Autoantibodies were present in the alveoli in high concentrations and colocalized with GM-CSF. They recognized human GM-CSF with high avidity (K(AV) = 20.0 +/- 7.5 pM) and high specificity, reacting with its superstructure and neutralizing GM-CSF activity to a level 4000 to 58 000 times the levels of GM-CSF normally present in the lung. Although target epitopes varied among patients, GM-CSF amino acids 78 to 94 were consistently recognized. Thus, autoantibodies bind GM-CSF with high specificity and high affinity, exist abundantly in the lung, and effectively block GM-CSF binding to its receptor, inhibiting AM differentiation and function. Our data strengthen the evidence associating anti-GM-CSF autoantibodies with the pathogenesis of this disease. 相似文献
927.
I Yamamoto I Iwasaki E Miyauchi S Azukizawa T Kigoshi K Uchida S Morimoto 《Nippon Naibunpi Gakkai zasshi》1990,66(1):42-49
Altered sodium intake is known to cause a greater change in plasma 18-hydroxycorticosterone (18-OHB) level than in plasma aldosterone level, resulting in an increase of plasma 18-OHB/aldosterone ratio in sodium-depleted man and rats. To evaluate the role of endogenous angiotensin II in the high plasma 18-OHB/aldosterone ratio in sodium-depleted rats, we examined the effect of the angiotensin I converting enzyme inhibitor SQ 14225 on plasma 18-OHB and aldosterone in sodium-depleted (SD) and sodium-repleted (SR) conscious rats. Plasma renin activity (PRA) and plasma angiotensin II were higher in the SD rats than in the SR rats. The ingestion of SQ 14225 caused an increase in PRA and a decrease in plasma angiotensin II, whereas these changes were more prominent in the SD rats than in the SR rats. Plasma 18-OHB and aldosterone levels were higher in the SD rats than in the SR rats. The plasma 18-OHB/aldosterone ratio was also higher in the SD rats than in the SR rats. The ingestion of SQ 14225 caused decreases in plasma 18-OHB and aldosterone levels in both the SR and SD rats, whereas the SQ 14225-induced decreases in plasma 18-OHB and aldosterone levels were more prominent in the SD rats than in the SR rats. Thus, the ingestion of SQ 14225 induced a decrease in the plasma 18-OHB/aldosterone ratio in both the SR and SD rats. The decrease in plasma 18-OHB/aldosterone ratio was more prominent in the SD rats than in the SR rats.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
928.
Reisaku Kono Akira Sasagawa Keizo Ishii Michinari Ochi Seiji Sugiura Hidemi Matsumiya Yukio Uchida Kazuko Kameyama Michiko Kaneko Nakao Sakurai 《Lancet》1972
A new type of conjunctivitis was prevailing in a pandemic fashion all over South-East Asia and Japan in 1970 and 1971. The disease is characterised by severe subconjunctival hæmorrhage, but its clinical course is usually benign and complete recovery comes within a fortnight. A cytopathic agent has been isolated by primary human embryonic kidney cell culture of conjunctival scrapings or swabs taken from patients with acute hæmorrhagic conjunctivitis (A.H.C.) in three different outbreaks in Japan. One of the earliest isolates which was chosen for detailed study showed general characteristics of enterovirus, but it was not neutralised by intersecting pools of antisera against known enteroviruses, and it was not pathogenic to suckling mice. It is suggested that the agent is an enterovirus of a new serotype. 10 pairs of acute and convalescent sera were tested, and there were definite neutralising antibody rises against this agent in all of them. No specific bacteria were found from cultures of conjunctival swabs. It is concluded that A.H.C. in Japan is caused by a hitherto unknown type of enterovirus and that outbreaks in other countries may be due to a similar agent. 相似文献
929.
Relationship among plasma iron, plasma iron turnover, and reticuloendothelial iron release 总被引:1,自引:0,他引:1
A circadian rhythm was demonstrated in 10 males and 10 females with respective mean decreases in plasma iron concentration at 18 hr of 62% and 47% of morning values. Ferrokinetic studies performed on 5 normal males and 5 normal females showed a more rapid disappearance rate and lower plasma iron turnover in the evening. Parallel studies were done on 6 normal males in the morning and 4 normal males in the evening of the release of reticuloendothelial iron at 8 and 18 hr after intravenous injection of 59Fe chondroitin ferrous sulfate. The 6-hr release in the morning was 54.1% and in the evening 25.9%. Composite data from morning and evening showed a correlation between plasma iron level and plasma iron turnover (r = 0.76, p less than 0.001). A similar correlation existed between the plasma iron level and the percent of radioiron released from the reticuloendothelial system (r = 0.67, 0.02 less than p less than 0.05). These data are consistent with a fluctuating iron release from the reticuloendothelial cell in normal subjects, which would account for the diurnal variation in plasma iron. 相似文献
930.