尼龙1010／6及尼龙1010／66动态力学分析

通过动态力学参数温度谱,研究了尼龙１０１０／６、尼龙１０１０／６６两个共聚物系列的动态力学参数与组成的关系。研究表明尼龙１０１０／６、尼龙１０１０／６６在测试温度范围内出现三个明显的松驰转变：α、β、γ,其中各共聚物的β、γ松驰温度相差不大,而α松驰温度随组成改变有明显改变。一般均聚物的α松驰温度较高,共聚物的α松驰温度均低于均聚物,二个系列以尼龙１０１０／６（２９．８／７０．２）、尼龙１０１０／     

Clonal growth of human acute leukemia cells in serum-free methylcellulose medium

Ｔｈｅｇｒｏｗｔｈｏｆｌｅｕｋｅｍｉａｉｎｓｅｍｉｓｏｌｉｄｃｕｌｔｕｒｅｉｎｖｉｔｒｏｗａｓｄｅｐｅｎｄｅｎｔｏｎｔｈｅｐｒｅｓ－ｅｎｃｅｏｆｅｘｏｇｅｎｏｕｓｓｅｒｕｍ．Ｓｅｒｕｍｉｓａｍｉｘ－ｔｕｒｅｃｏｎｔａｉｎｉｎｇｌａｒｇｅｎｕｍｂｅｒｏｆｕｎｉｄｅｎｔｉｆｉｅｄｃｈｅｍｉｃａｌｃｏｍｐｏｎｅｎｔｓａｎｄｕｎｉｄｅｎｔｉｆｉｅｄｂｉｏ－ｌｏｇｉｃａｌｌｙａｃｔｉｖｅｓｕｂｓｔａｎｃｅｓ［１］,ｗｈｉｃｈｐｒｏｖｉｄｅｔｈｅｎｕｔｒｉｅｎｔｓｎｅｃｅｓｓａｒｙｆｏｒｔｈｅｃｅｌｌｇｒｏ…     

Reduction of tumorigenicity by an interferon-γ-gene-transduced tumor on another syngeneic tumor in a murine model

To evaluate the effect of interferon-γ-genetransduced cells, DS mice were inoculated into their footpads with syngeneic mammary adenocarcinoma SC42 admixed with interferon-γ producing mammary adenocarcinoma SC115Kγ, which had been established by an interferon-γ-gene transduction in another syngeneic mammary adenocarcinoma SC115 using retroviral vectors. These mice rejected both tumor cells and developed resistance to subsequent challenges with either SC115 or SC42 cells inoculated into their opposite posterior footpads. These results thus indicate that systemic immunological memory to each of the independent tumor cell lines developed in these mice. Although the SC42 cells admixed with irradiated SC115Kγ cells were rejected by these mice, the SC42 cells admixed with irradiated SC115neoR, in which the neo-gene had been transduced, were observed to proliferate. Tumor rejection was reversed by an in vivo administration of anti-interferon-γ antibody, thus suggesting that locally produced interferon-γ plays an important role in tumor elimination and immunological memory induction. In conclusion, interferon-γ-gene-transduced tumor cells are therefore considered to have a therapeutic potential for other types of malignant tumor cell lines.     

P2u receptor-mediated release of endothelium-derived relaxing factor/nitric oxide and endothelium-derived hyperpolarizing factor from cerebrovascular endothelium in rats

BACKGROUND AND PURPOSE: Stimulation of P2u purinoceptors by UTP on endothelium dilates the rat middle cerebral artery (MCA) through the release of endothelium-derived relaxing factor/nitric oxide (EDRF/NO) and an unknown relaxing factor. The purpose of this study was to determine whether this unknown relaxing factor is endothelium-derived hyperpolarizing factor (EDHF). METHODS: Rat MCAs were isolated, cannulated, pressurized, and luminally perfused. UTP was added to the luminal perfusate to elicit dilations. RESULTS: Resting outside diameter of the MCAs in one study was 209+/-7 micrometer (n=10). The MCAs showed concentration-dependent dilations with UTP administration. Inhibition of NO synthase with NG-nitro-L-arginine methyl ester (L-NAME) (1 micromol/L to 1 mmol/L) did not diminish the maximum response to UTP but did shift the concentration-response curve to the right. Scavenging NO with hemoglobin (1 or 10 micromol/L) or inhibition of guanylate cyclase with ODQ (1 or 10 micromol/L) had effects on the UTP-mediated dilations similar to those of L-NAME. In the presence of L-NAME, dilations induced by 10 micromol/L UTP were accompanied by 13+/-2 mV (P<0.009) hyperpolarization of the vascular smooth muscle membrane potential (-28+/-2 to -41+/-1 mV). Iberiotoxin (100 nmol/L), blocker of the large-conductance calcium-activated K channels, sometimes blocked the dilation, but its effects were variable. Charybdotoxin (100 nmol/L), also a blocker of the large-conductance calcium-activated K channels, abolished the L-NAME-insensitive component of the dilation to UTP. CONCLUSIONS: Stimulation of P2u purinoceptors on the endothelium of the rat MCA released EDHF, in addition to EDRF/NO, and dilated the rat MCA by opening an atypical calcium-activated K channel.     

Neurothekeoma palpebrae: a rare nerve sheath tumor arising in the eyelid

PURPOSE: To report a case of an eyelid neurothekeoma, a rare peripheral nerve sheath tumor. METHODS: Case report. RESULTS: An excisional biopsy, performed on a lesion removed from the upper eyelid of a 76-year-old woman, revealed the clinical and histopathologic features of a neurothekeoma, a tumor consisting of multiple collections of spindle cells in a myxomatous background. Immunohistochemical characterization showed positivity for NK1/C3, neuron-specific enolase, and alcian blue. CONCLUSION: Neurothekeoma palpebrae should be considered in the differential diagnosis of solitary nodules of the eyelids.     

大剂量甲基强的松龙静脉内冲击疗法治疗顽固性巩膜炎

评估甲基强的松龙对严重顽固性巩膜炎的疗效,为治疗严重顽固性巩膜炎寻找一种理想的方法。方法：１０例严重顽固性巩膜炎患者采用静脉内大剂量甲基强的松龙冲击疗法（５００ｍｇ／日）,连续３天为一疗程,可重复几个疗程,每疗程后间隔３～４天,并逐渐减量。结果：３～４疗程后,１０例患者其平均记分由治疗前的１２．２分降低到２．４分（Ｐ＜０．０１）,病情改善者３例,治愈者７例。副反应轻微。结论：静脉内大剂量甲基强的松龙冲击疗法治疗巩膜炎疗效高、作用快、副反应轻,是一种理想的治疗方法。值得进一步总结。     

电针对去势大鼠外周血淋巴细胞雌激素受体影响的研究

目的　从生殖内分泌 -免疫网络角度探讨电针治疗围绝经期综合征的作用机制。方法　以去势大鼠为实验对象 ,取双侧三阴交、太溪、后三里穴 ,采用放射免疫法和流式细胞分析技术 ,观察电针治疗对去势大鼠血清雌激素 (E2 )、促黄体生成素 (LH)、促卵泡生成素(FSH)及外周血淋巴细胞内雌激素受体 (ER)水平的影响。结果　电针可使去势大鼠血清E2 明显升高 ,LH及FSH明显下降 ,外周血淋巴细胞内ER明显升高 ,与造模组比较有显著性差异。结论　电针可明显改善去势大鼠的生殖内分泌功能 ,并能调节免疫细胞的雌激素受体表达。     

The attitudes of cancer patients and their families toward the disclosure of terminal illness.

PURPOSE:To ascertain the attitude of cancer patients and their families toward disclosure of terminal illness to the patient. PATIENTS AND METHODS: We constructed a questionnaire that included demographic and clinical information and delivered it to 758 consecutive individuals (433 cancer patients and 325 families that have a relative with cancer) at seven university hospitals and one national cancer center in Korea. RESULTS: 380 cancer patients and one member from each of 281 families that have a relative with cancer completed the questionnaire. Cancer patients were more likely than family members to believe that patients should be informed of the terminal illness (96.1% v 76.9%; P <.001). Fifty percent of the family members and 78.3% of the patients thought that the doctor in charge should be the one who informs the patient. Additionally, 71.7% of the patients and 43.6% of the family members thought that patients should be informed immediately after the diagnosis. Stepwise multiple logistic regression indicated that the patient group was more likely than the family group to want the patient to be informed of the terminal illness (odds ratio [OR], 9.76; 95% CI, 4.31 to 22.14), by the doctor (OR, 4.00; 95% CI, 2.61 to 6.11), and immediately after the diagnosis (OR, 3.64; 95% CI, 2.45 to 5.41). CONCLUSION: Our findings indicated that most cancer patients want to be informed if their illness is terminal, and physicians should realize that the patient and the family unit may differ in their attitude toward such a disclosure. Our results also reflect the importance of how information is given to the patient.