A longitudinal study of semen quality in pesticide spraying danish farmers

It was hypothesized that occupational exposure to pesticides during a spraying season causes changes in semen quality that might be detected in a longitudinal study. We analyzed the within-person changes in semen quality and reproductive hormones across a spraying season in groups of farmers using and not using pesticides. A total of 248 men collected two semen samples (participation rate: 32%). The median sperm concentration declined significantly from the first to the second sample in both groups, but there was no statistical difference in the decline between the two groups, unadjusted or adjusted. Only minor changes were found in sperm morphology, vitality, motility, sperm chromatin denaturation (SCSA), and reproductive hormones, and the differences in changes between the two groups were nonsignificant, or, in the opposite direction to the expected. There was no relation between the changes in sperm parameters in relation to pesticide exposure variables. In conclusion, use of pesticides by Danish farmers is not a likely cause of short-term effects on semen quality and reproductive hormones.     

神经根慢性嵌压损伤的动物模型建立

目的：建立一种由自身骨性增生造成神经根慢性嵌压损伤的动物模型，为相关实验提供造模方法一方法：30只健康家猫，手术显露右侧C7、C8和L5、L6冲经根及其椎问孔内口，用牙髓钻破坏椎间孔周围骨皮质后，将“V”形松质骨块沿骨壁嵌于神经根通道的骨性管道内及侧隐窝后方，左侧做正常对照。在造模术前和术后第2、4、8、12、24周行磁刺激运动诱发电位(MEP)检测，每次随机选6只，4～5只行影像学检查，以确定神经根通道的狭窄程度和神经根受压状态，6只均做病理组织学检查和椎间孔截面积测量。结果：术后早期实验侧肢体出现行为异常：而后有不同程度肌萎缩；后期部分肢体远端出现溃疡。影像学检查随着嵌压时间延长．实验侧椎间孔骨痂增多，狭窄加重。神经根受压变形，椎间孔骨性截面积8周后明显减小，与对照侧比较有显著性差异。术后2周，神经根组织学检查主要表现为神经束膜、内膜的水肿。髓鞘肿胀；4周后发生节段性脱髓鞘：8周时神经轴突增粗、断裂，远端瓦勒氏变性；12周后变性冲经结构崩解、吸收，形成空洞：24周时整个神经干纤维化。术后4周时实验侧MEP开始出现潜伏期延长；8周时伴有波形分化不清；12周波幅明显下降：24周MEP的引出困难，部分电位消失。结论：采用椎问孔内自体松质骨植入，造成神经根慢性嵌压性损伤模型成功率高，操作简单，适用于脊柱各个节段，其损伤部位和形式与临床更为接近。     

病毒白细胞介素-10转基因在造血干细胞中的表达

目的　观察逆转录病毒转病毒白细胞介素 10 (vIL 10 )基因在体内的表达。方法　用MSCVneo vIL 10重组体在体外转导CBA (H 2 K)小鼠的造血干细胞 (HSCs) ,给经致死照射(90 0rads)的 2 0只同基因CBA(H 2 K)小鼠注入经MSCVneo vIL 10转染的HSCs ,2× 10 6HSCs/只。酶联免疫吸附测定 (ELISA)、逆转录 聚合酶链反应 (RT PCR )、Westernblot分析vIL 10的表达。结果　移植MSCVneo vIL 10转染HSCs的 2 0只小鼠 ,移植后 8周用ELISA检测 ,其中 15只小鼠血清的vIL 10浓度为 :2 70～ 13 40ng/L ,5只小鼠血清的vIL 10为阴性。 12周后有 2只小鼠vIL 10测不出 ,13只小鼠长时间表达vIL 10达 6个月。对照组小鼠血清vIL 10均为阴性。RT PCR和Westernblot证实小鼠的器官均有vIL 10的mRNA和蛋白的表达。结论　逆转录病毒能有效地将vIL 10基因导入造血干细胞并在体内长时间表达。     

Structural and ultrastructural characteristics of human pineal gland,and pineal parenchymal tumors

We have studied 20 pineal parenchymal tumors (PPT) and 4 normal or cystic pineal glands both by light and electron microscopy and immunohistochemistry with antibodies against glial markers [glial fibrillary acidic protein (GFAP) and protein S-100] or neural/neuroendocrine markers [neurofilaments (NF), synaptophysin and chromogranin A]. Light microscopy revealed the cellular organization of pinealocytes in the normal gland and in different morphological types of pineal tumors (typical pineocytomas, PPT with intermediate differentiation, mixed PPT exhibiting elements of both pineocytoma and pineoblastoma and pineoblastomas). Immunohistochemistry showed the presence of GFAP and protein S-100 in interstitial cells in nonneoplastic pineal gland. Cell processes were labeled with anti-synaptophysin and anti-NF antibodies. No immunoreactivity was found for chromogranin A in non-neoplastic pineal gland. In pineocytomas, GFAP and protein S-100 were observed in interstitial cells. Synaptophysin and NF were present in the large rosettes of pineocytomas. Synaptophysin, NF and chromogranin A were present in pineocytomas with a lobular arrangement of cells. Anti-chromogranin A immuno-reactivity was also seen in lobular areas of some PPT with intermediate differentiation. Analysis of normal human pineal gland by electron microscopy showed the presence of vesicle-crowned rodlets (VCR or synaptic ribbons), fibrous filaments (F), paired twisted filaments but few dense-core vesicles (DCV) in normal pinealocytes. Tumoral pineal cells appeared to differentiate either towards a neurosensory pathway characterized by the presence of sensory cells elements (VCR and F), or towards a neuroendocrine pathway, with the occurrence of many DCV. Immunogold labeling demonstrated the presence of chromogranin A in neurosecretory granules.Supported by grants from the Région Rhône Alpes and from INSERM (CJF 90-10)     

再生医学技术在烧伤传统治疗无效病人中的应用(附38例报告)

目的：总结重度烧伤传统疗法无效改为再生医学(MEBT/MEB0)技术治疗变化规律和临床验．方法：将我科l995年5月至2002年5月收治的院外采用传统疗法无效改用MEBT/MEBO技术治疗的38例重度烧伤病人临床资料进行回顾性总结，病人一旦入院创面均改用MEBT/MEBO治疗，初始予以彻底清创，规范用药、规范操作，规范认识；全身实行系统综合治疗措施．以临床观察和病人感觉评价治疗效果。结果：本组38例全部治愈，末植皮自行愈合者21例．占55.26％．深Ⅲ度创面自愿要求植皮者17例，占44.74％。经随访多数无增生性瘢痕，部分愈后有局限性瘢癌，质软无残废。结论：重度烧伤经传统疗法久治不愈或疗效欠佳病人．病情复杂，并发症较多治疗难度也较大，再生医学可有效改善以上缺陷和病症．是重度烧伤病人传统治疗无效时的理想疗法。     

Multiple occult vascular malformations of the brain and spinal cord: MRI diagnosis

Summary We report a patient with multiple angiographically occult vascular malformations in the brain and spine. Magnetic resonance imaging showed multiple lesions in brain and spine with hypointense areas on both T1 and T2-weighted images. These hypointense areas are usually secondary to hemosiderin deposits consistent with remote bleeding in the lesions. We conclude that when magnetic resonance reveals an intraspinal lesion with signal intensity characteristics consistent with a vascular malformation, an examination of the brain should be performed to rule out associated intracranial lesions. The finding of multiple lesions in the brain with identical signal intensity characteristics reinforces the diagnosis of vascular malformation.     

胶态果胶铋对实验性大鼠急性胃炎及兔慢性胃炎疗效的观察

果胶铋是一种新型的铋制剂，实验发现：０．５ｇ／ｋｇ／ｄ果胶铋灌胃大鼠能够明显减轻由ＨＣＩ引起的急性胃炎胃粘膜的病变程度；同时还发现，０．２５／ｋｇ／ｄ果胶铋灌胃家兔对兔实验性变态反应性胃炎有明显的治疗作用，能显著减小病变面积，减轻或消除胃粘膜炎症，且未见溃疡发生。其疗效与得乐疗效相比无显著性差异。     

Ebstein畸形82例心血管造影分析

报道我院1958～1992年期间的 Ebstein 畸形82例。平片表现可归纳为球型、方型和不典型4种心脏形态。介绍该畸形的心血管造影方法,所见征象和相应的病理基础。主要造影征象有:1.心脏膈面双切迹征;2.帆样征:3.房化心室:4.三尖瓣闭锁不全。本文结合文献对本病病理,X 线表现进行了系统的讨论,并着重突出了我们的观点。     

Pharmacokinetics of famotidine in patients with cirrhosis and ascites

Summary The pharmacokinetics of famotidine has been investigated in ascitic cirrhotic patients. 10 decompensated cirrhotic patients were studied (9 m, 1 f), who had normal renal function, and six healthy control subjects (4 m, 2 f), matched for age, sex and weight. Each subject received on two occasions, at least four days apart, a single oral (40 mg) or intravenous dose (20 mg) of famotidine, at 21.00 h in a randomised manner. Serial blood samples were collected and famotidine in plasma was determined by a HPLC/UV method. Plasma data were subjected to non compartmental pharmacokinetic analysis.There were no statistically significant differences in pharmacokinetic parameters between the two groups after either the intravenous or oral administration of famotidine.The findings suggest that the dose of famotidine may not require any adjustment in ascitic patients without renal failure.     

钡离子(Ba~■)诱发心室肌细胞自发电活动的实验

用心肌细胞跨膜电位记录,证实高浓度Ba~■(4mol/L)可诱发豚鼠心室肌自发电活性,这种自发电活性主要来自延迟后除极电位(DAD)及触发活动,且可被维拉帕米(异搏定)阻断,提示DAD的产生与钙离子内流有关。