全文获取类型
收费全文 | 1523篇 |
免费 | 44篇 |
国内免费 | 5篇 |
专业分类
耳鼻咽喉 | 18篇 |
儿科学 | 18篇 |
妇产科学 | 58篇 |
基础医学 | 210篇 |
口腔科学 | 9篇 |
临床医学 | 104篇 |
内科学 | 370篇 |
皮肤病学 | 37篇 |
神经病学 | 111篇 |
特种医学 | 36篇 |
外科学 | 142篇 |
综合类 | 9篇 |
预防医学 | 71篇 |
眼科学 | 11篇 |
药学 | 145篇 |
中国医学 | 19篇 |
肿瘤学 | 204篇 |
出版年
2023年 | 5篇 |
2022年 | 31篇 |
2021年 | 51篇 |
2020年 | 22篇 |
2019年 | 18篇 |
2018年 | 14篇 |
2017年 | 15篇 |
2016年 | 17篇 |
2015年 | 34篇 |
2014年 | 29篇 |
2013年 | 57篇 |
2012年 | 86篇 |
2011年 | 81篇 |
2010年 | 59篇 |
2009年 | 47篇 |
2008年 | 87篇 |
2007年 | 112篇 |
2006年 | 122篇 |
2005年 | 138篇 |
2004年 | 123篇 |
2003年 | 109篇 |
2002年 | 109篇 |
2001年 | 15篇 |
2000年 | 6篇 |
1999年 | 12篇 |
1998年 | 12篇 |
1997年 | 5篇 |
1996年 | 8篇 |
1995年 | 5篇 |
1994年 | 8篇 |
1993年 | 5篇 |
1992年 | 15篇 |
1991年 | 6篇 |
1987年 | 6篇 |
1986年 | 5篇 |
1985年 | 6篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 6篇 |
1981年 | 4篇 |
1979年 | 4篇 |
1978年 | 6篇 |
1977年 | 6篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1971年 | 7篇 |
1966年 | 3篇 |
1964年 | 3篇 |
排序方式: 共有1572条查询结果,搜索用时 15 毫秒
91.
High-resolution whole-organ mapping with SNPs and its significance to early events of carcinogenesis 总被引:3,自引:0,他引:3
Tuziak T Jeong J Majewski T Kim MS Steinberg J Wang Z Yoon DS Kuang TC Baggerly K Johnston D Czerniak B 《Laboratory investigation; a journal of technical methods and pathology》2005,85(5):689-701
We attempted to identify deleted segments in two model tumor suppressor gene loci on chromosomes 13q14 and 17p13 that were associated with clonal expansion of in situ bladder preneoplasia using single nucleotide polymorphisms (SNPs)-based whole-organ histologic and genetic mapping. For mapping with SNPs, the sequence-based maps spanning approximately 27 and 5 Mb centered around RB1 and p53, respectively, were assembled. The integrated gene and SNP maps of the regions were used to select 661 and 960 SNPs, which were genotyped by pyrosequencing. Genotyping of SNPs was performed on DNA samples corresponding to histologic maps of the entire bladder mucosa in human cystectomy specimens with invasive urothelial carcinoma. By using this approach, we have identified deleted regions associated with clonal expansion of intraurothelial neoplasia; which ranged from 0.001 to 4.3 Mb (average 0.67 Mb) and formed clusters of discontinuous deleted segments. The high resolution of such maps is a prerequisite for future positional targeting of genes involved in early phases of bladder neoplasia. This approach also permits analysis of the overall genomic landscape of the involved region and discloses that a unique composition of noncoding DNA characterized by a high concentration of repetitive sequences may predispose to deletions. 相似文献
92.
93.
Background. The following study is an attempt to draw the readers' attention to the issue of the multifariousness of the intervertebral discs' destructive processes resulting in the neuro-vertebral syndrome. The professional literature mentioned here gives a prominence to the thread of immunological phenomena accompanying disc diseases and their treatment.
Material and methods. Selected groups of patients with a high probability of the coexistence of immunological processes were analyzed in terms of pain patterns and topographic cross-sections of the intervertebral discs. They were then treated transdermically with butapirazole and levamisole hydrochloride under the control of Lasegu's bioengineer test, electro-sensory tests and CIC level tests.
Results. A Lasegu's test, an electro-sensory (I) test and a survey showed that the groups of patients treated locally 15 times with Butapirazole showed an almost complete recovery of the symmetry with no effect on the CIC level and the localized pain intensity (II). The majority of the patients experienced a regression of ailments within 3 months. As far as the group of patients treated with Levamisole is concerned both root (I) and localized pains (II) subsided rapidly as confirmed by a survey and a Lasegu's test as well as the electro-sensory tests (I), (II). A high decrease of the level of immunological complexes was noticed after only 15 Levamisole infusions. This correct level was maintained 3 months later, which was shown by further tests.
Conclusions. This seems to be the premise confirming the effect of a localized inflammatory point on the level of immunological complexes present in blood (CIC). 相似文献
Material and methods. Selected groups of patients with a high probability of the coexistence of immunological processes were analyzed in terms of pain patterns and topographic cross-sections of the intervertebral discs. They were then treated transdermically with butapirazole and levamisole hydrochloride under the control of Lasegu's bioengineer test, electro-sensory tests and CIC level tests.
Results. A Lasegu's test, an electro-sensory (I) test and a survey showed that the groups of patients treated locally 15 times with Butapirazole showed an almost complete recovery of the symmetry with no effect on the CIC level and the localized pain intensity (II). The majority of the patients experienced a regression of ailments within 3 months. As far as the group of patients treated with Levamisole is concerned both root (I) and localized pains (II) subsided rapidly as confirmed by a survey and a Lasegu's test as well as the electro-sensory tests (I), (II). A high decrease of the level of immunological complexes was noticed after only 15 Levamisole infusions. This correct level was maintained 3 months later, which was shown by further tests.
Conclusions. This seems to be the premise confirming the effect of a localized inflammatory point on the level of immunological complexes present in blood (CIC). 相似文献
94.
95.
David John Faulkner, one of the pioneers of marine natural products chemistry and the 2003 recipient of the ASP Research Achievement Award, passed away on November 23, 2002. John was very pleased to learn that he'd been named as the award recipient and was intending to present the ASP Research Achievement Award Address at the annual meeting in July 2003, Chapel Hill, North Carolina. John's untimely death left an unprecedented event in the history of the ASP-a posthumous Research Achievement Award to a deserving individual and an untimely loss for us all. We are bereft of a colleague, a friend, and a mentor, and the opportunity to hear John's words on the occasion of his own award. In tribute to John, we have assembled a retrospective of John's work that is not meant to be a comprehensive review (this would take considerably more space) but a selection of highlights and personal vignettes from some of those that trained in marine natural products under his mentorship. This paper is a written account of the symposium presented by the authors at the ASP Annual Meeting in Chapel Hill, North Carolina, on July 16, 2003. 相似文献
96.
Kossuga MH MacMillan JB Rogers EW Molinski TF Nascimento GG Rocha RM Berlinck RG 《Journal of natural products》2004,67(11):1879-1881
A new antifungal agent, (2S,3R)-2-aminododecan-3-ol (1), has been isolated from the ascidian Clavelina oblonga collected in Brazil. The structure of 1 was established by analysis of spectroscopic data, including absolute stereochemistry determined by circular dichroism analysis of the dibenzoyl derivative 2. Compound 1 displayed antifungal activity against Candida albicans ATCC 10231 with a MIC of 0.7 mug/mL and against Candida glabrata with a MIC of 30 microg/mL. 相似文献
97.
98.
Rogalińska M Błoński JZ Hanausek M Walaszek Z Robak T Kiliańska ZM 《Cancer Detection and Prevention》2004,28(6):433-442
The purpose of the study was to determine some apoptotic events in mononuclear cells obtained from peripheral blood of patients with B-cell chronic lymphocytic leukemia (B-CLL) during and after therapy with 2-chlorodeoxyadenosine (2-CdA; C), and the combination of 2-CdA with cyclophosphamide (CC), or 2-CdA with mitoxantrone and cyclophosphamide (CMC). Western blot technique was performed to estimate expression/proteolytic degradation of generally accepted apoptotic markers, i.e., Bcl-2 protein, lamin B, PARP-1, and caspase-3 in leukemic cells isolated from blood samples of patients before treatment and subjected to drug(s) administration. The decrease of antiapoptotic protein Bcl-2 expression and proteolytic cleavage of nuclear proteins--lamin B and PARP-1 were observed in leukemic cells of patients treated according to the above therapy protocols, however, each to a different level among the studied groups. The obtained results indicated also that procaspase-3 was cleaved and activated in leukemic cells of three drug(s) treated groups. However, the cleavage of procaspase-3 and the generation of fragments with mol. mass of 17/20 kDa occurred especially effectively among patients treated according to CMC regimen. The changes in expression/proteolytic degradation of the above selected apoptotic markers, are accompanied by the appearance of apoptotic morphology in leukemic cells originated from blood of patients treated with the above drug(s) in comparison to untreated ones. 相似文献
99.
100.