全文获取类型
收费全文 | 591篇 |
免费 | 29篇 |
国内免费 | 7篇 |
专业分类
耳鼻咽喉 | 3篇 |
儿科学 | 37篇 |
妇产科学 | 3篇 |
基础医学 | 44篇 |
口腔科学 | 19篇 |
临床医学 | 46篇 |
内科学 | 146篇 |
皮肤病学 | 8篇 |
神经病学 | 10篇 |
特种医学 | 85篇 |
外科学 | 71篇 |
综合类 | 64篇 |
预防医学 | 19篇 |
眼科学 | 9篇 |
药学 | 33篇 |
中国医学 | 1篇 |
肿瘤学 | 29篇 |
出版年
2024年 | 1篇 |
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 10篇 |
2020年 | 7篇 |
2019年 | 8篇 |
2018年 | 9篇 |
2017年 | 4篇 |
2016年 | 11篇 |
2015年 | 17篇 |
2014年 | 13篇 |
2013年 | 20篇 |
2012年 | 20篇 |
2011年 | 15篇 |
2010年 | 23篇 |
2009年 | 33篇 |
2008年 | 17篇 |
2007年 | 29篇 |
2006年 | 34篇 |
2005年 | 10篇 |
2004年 | 12篇 |
2003年 | 4篇 |
2002年 | 8篇 |
2001年 | 11篇 |
2000年 | 7篇 |
1999年 | 13篇 |
1998年 | 42篇 |
1997年 | 31篇 |
1996年 | 40篇 |
1995年 | 17篇 |
1994年 | 18篇 |
1993年 | 22篇 |
1992年 | 4篇 |
1991年 | 8篇 |
1990年 | 2篇 |
1989年 | 9篇 |
1988年 | 24篇 |
1987年 | 9篇 |
1986年 | 8篇 |
1985年 | 17篇 |
1984年 | 7篇 |
1983年 | 4篇 |
1982年 | 3篇 |
1981年 | 3篇 |
1980年 | 4篇 |
1977年 | 3篇 |
1976年 | 5篇 |
1975年 | 3篇 |
排序方式: 共有627条查询结果,搜索用时 15 毫秒
51.
52.
53.
KD McCloskey UA Anderson RA Davidson YR Bayguinov KM Sanders SM Ward 《British journal of pharmacology》2009,156(2):273-283
Background and purpose:
W/Wv and wild-type murine bladders were studied to determine whether the W/Wv phenotype, which causes a reduction in, but not abolition of, tyrosine kinase activity, is a useful tool to study the function of bladder interstitial cells of Cajal (ICC).Experimental approach:
Immunohistochemistry, tension recordings and microelectrode recordings of membrane potential were performed on wild-type and mutant bladders.Key results:
Wild-type and W/Wv detrusors contained c-Kit- and vimentin-immunopositive cells in comparable quantities, distribution and morphology. Electrical field stimulation evoked tetrodotoxin-sensitive contractions in wild-type and W/Wv detrusor strips. Atropine reduced wild-type responses by 50% whereas a 25% reduction occurred in W/Wv strips. The atropine-insensitive component was blocked by pyridoxal-5-phosphate-6-azophenyl-2′,4′-disulphonic acid in both tissue types. Wild-type and W/Wv detrusors had similar resting membrane potentials of −48 mV. Spontaneous electrical activity in both tissue types comprised action potentials and unitary potentials. Action potentials were nifedipine-sensitive whereas unitary potentials were not. Excitatory junction potentials were evoked by single pulses in both tissues. These were reduced by atropine in wild-type tissues but not in W/Wv preparations. The atropine-insensitive component was abolished by pyridoxal-5-phosphate-6-azophenyl-2′,4′-disulphonic acid in both preparations.Conclusions and implications:
Bladders from W/Wv mice contain c-Kit- and vimentin-immunopositive ICC. There are similarities in the electrical and contractile properties of W/Wv and wild-type detrusors. However, significant differences were found in the pharmacology of the responses to neurogenic stimulation with an apparent up-regulation of the purinergic component. These findings indicate that the W/Wv strain may not be the best model to study ICC function in the bladder. 相似文献54.
Calvin KY Cheng Eric HY Lau Dennis KM Ip Alfred SY Yeung Lai Ming Ho Benjamin J Cowling 《BMC public health》2009,9(1):339
Background
Influenza surveillance systems provide important and timely information to health service providers on trends in the circulation of influenza virus and other upper respiratory tract infections. Online dissemination of surveillance data is useful for risk communication to health care professionals, the media and the general public. We reviewed national influenza surveillance websites from around the world to describe the main features of surveillance data dissemination. 相似文献55.
Novel vanilloid receptor-1 antagonists: 1. Conformationally restricted analogues of trans-cinnamides
Norman MH Zhu J Fotsch C Bo Y Chen N Chakrabarti P Doherty EM Gavva NR Nishimura N Nixey T Ognyanov VI Rzasa RM Stec M Surapaneni S Tamir R Viswanadhan VN Treanor JJ 《Journal of medicinal chemistry》2007,50(15):3497-3514
The vanilloid receptor-1 (VR1 or TRPV1) is a member of the transient receptor potential (TRP) family of ion channels and plays a role as an integrator of multiple pain-producing stimuli. From a high-throughput screening assay, measuring calcium uptake in TRPV1-expressing cells, we identified an N-aryl trans-cinnamide (AMG9810, compound 9) that acts as a potent TRPV1 antagonist. We have demonstrated the antihyperalgesic properties of 9 in vivo and have also reported the discovery of novel, orally bioavailable cinnamides derived from this lead. Herein, we expand our investigations and describe the synthesis and biological evaluation of a series of conformationally constrained analogues of the s-cis conformer of compound 9. These investigations resulted in the identification of 4-amino- and 4-oxopyrimidine cores as suitable isosteric replacements for the trans-acrylamide moiety. The best examples from this series, pyrimidines 79 and 74, were orally bioavailable and exhibited potent antagonism of both rat (IC50 = 4.5 and 0.6 nM, respectively) and human TRPV1 (IC50 = 7.4 and 3.7 nM, respectively). In addition, compound 74 was shown to be efficacious at blocking a TRPV1-mediated physiological response in vivo in the capsaicin-induced hypothermia model in rats; however, it was ineffective at preventing thermal hyperalgesia induced by complete Freund's adjuvant in rats. 相似文献
56.
57.
A Lally D Casabonne T Waterboer B Imko‐Walczuk KM Michael M Pawlita R Newton F Wojnarowska 《Journal of the European Academy of Dermatology and Venereology》2010,24(3):302-307
Background Renal transplant recipients (RTR) have a well recognized increased risk of cutaneous malignancy. A clinical observation that RTR with skin cancer often had multiple seborrhoeic warts prompted an investigation in RTR into the relationship between seborrhoeic warts and skin cancer and an exploration into potential risk factors for seborrhoeic warts in this population, including infection with human papillomavirus (HPV). Methods This was a case control study involving 308 RTR. Clinical examinations identified seborrhoeic warts. Histological records reviewed to look for evidence of prior cutaneous malignancy. Seroprevalence of antibodies to 34 different HPV types tested using multiplex serology. Odds ratios (OR) calculated using unconditional logistic regression analysis to look for associations between skin cancer, HPV infection and seborrhoeic warts, controlling for potential confounding factors of gender, age and time since transplantation. Results Seborrhoeic warts were associated with non‐melanoma skin cancer [OR = 3.7; 95% confidence intervals (CI) ranging from 1.6–8.9; P = 0.002] when confounding factors of gender, age and time since transplantation were controlled for. There was also an association between seborrhoeic warts and viral warts (OR = 3.0, CI: 1.6–5.4; P < 0.0001), but no association between seborrhoeic warts and infection with single or multiple HPV types. Conclusions Seborrhoeic warts are associated with cutaneous malignancy, but not with any of the HPV types tested. The reasons for this association are unclear. RTR with multiple seborrhoeic warts may require more regular cutaneous examination to monitor for early signs of skin cancer. 相似文献
58.
Abstract: In hemodialysis, a certain degree of bacterial contamination on the dialysate side is a regular finding. Concern has been growing that this contamination may lead to a chronic inflammatory response in the patient. Ultrafiltration of dialysate can be used to reduce bacterial content and levels of cytokine-inducing substances upstream of the patient's dialyzer. The aim of this study was to test in vitro the rejection capacity of a polysulfone hollow-fiber ultrafilter (ETF 609, NISSHO Co., Osaka, Japan) challenged with bacterial filtrates derived from Pseudomonus aeruginosa PA 103. Results showed a reduction of interleukin-Iβ-inducing activity (measured on peripheral blood mononuclear cells) from 5,035 ± 394 pg/ml prefilter to nondetectable levels postfilter and endotoxin levels (limulus amebocyte lysate assay) of 4,167 ± 1,079 versus 12 ± 2 pg/ml, respectively. In conclusion, ultrafiltration of dialysate with the polysulfone ultrafilter ETF 609 leads to a potent reduction of cytokine-inducing activity. 相似文献
59.
60.