c-myc反义寡核苷酸对HL-60细胞增殖及c-myc基因表达的影响

目的：观察c-myc基因反义寡核苷酸(ASODN)对HL-60细胞增殖及c-myc基因表达的抑制作用．探讨ASODN作为药物进行白血病基因治疗的可行性、方法：应用c-myc反义寡核苷酸封闭HL-60细胞c-myc基因、RTPCR方法检测该基因表达抑制情况．流式细胞仪进行细胞周期分析,倒置显微镜观察细胞形态变化．绘制细胞生长曲线,MTT法检测细胞增殖状态。结果：c-myc基因反义寡核苷酸转染HL-60细胞后,c-myc基因表达明显受抑;S期细胞比值由55.6％降至16．7％;细胞生长受抑,增殖能力减弱,其抑制作用具有序列特异性及剂量依赖性。结论c-myc基因反义寡核苷酸对HKL-60细胞增殖及c-myc基因的表达具有明显的抑制作用,有望成为抑制白血病细胞增殖的核酸药物。     

A Th2-score in the tumor microenvironment as a predictive biomarker of response to Bacillus Calmette Guérin in patients with non-muscle invasive bladder carcinoma: A retrospective study

Intravesical Bacillus Calmette Guerin (BCG) is the gold standard therapy for intermediate/high-risk nonmuscle invasive bladder cancer (NMIBC). However, the response rate is ~60%, and 50% of non-responders will progress to muscle-invasive disease. BCG induces massive local infiltration of inflammatory cells (Th1) and ultimately cytotoxic tumor elimination. We searched for predictive biomarker of BCG response by analyzing tumor-infiltrating lymphocyte (TIL) polarization in the tumor microenvironment (TME) in pre-treatment biopsies. Pre-treatment biopsies from patients with NMIBC who received adequate intravesical instillation of BCG (n = 32) were evaluated retrospectively by immunohistochemistry. TME polarization was assessed by quantifying the T-Bet+ (Th1) and GATA-3+ (Th2) lymphocyte ratio (G/T), and the density and degranulation of EPX+ eosinophils. In addition, PD-1/ PD-L1 staining was quantified. The results correlated with BCG response. In most non-responders, Th1/Th2 markers were compared in pre-and post-BCG biopsies. ORR was 65.6% in the study population. BCG responders had a higher G/T ratio and a greater number of degranulated EPX+ cells. Variables combined into a Th2-score showed a significant association with higher scores in responders (p = 0.027). A Th2-score cut-off value >48.1 allowed discrimination of responders with 91% sensitivity but lower specificity. Relapse-free survival was significantly associated with the Th2-score (p = 0.007). In post-BCG biopsies from recurring patients, TILs increased Th2-polarization, probably reflecting BCG failure to induce a pro-inflammatory status and, thus, a lack of response. PD-L1/PD-1 expression was not associated with the response to BCG. Our results support the hypothesis that a preexisting Th2-polarized TME predicts a better response to BCG, assuming a reversion to Th1 polarization and antitumor activity.     

中药多途径治疗溃疡性结肠炎的疗效及对炎症因子的影响

目的 研究中药多途径治疗溃疡性结肠炎的疗效及对炎症因子的影响。方法 选取2016年2月-2017年11月收治的136例溃疡性结肠炎患者,按随机数表法分成对照组（美沙拉嗪口服联合灌肠治疗）与研究组（中药煎液内服+中药灌肠+中药穴位贴敷治疗）,各68例。分析2组治疗前和治疗12周后炎症因子水平、T淋巴细胞亚群水平（CD₃⁺、CD₄⁺、CD₈⁺、CD₄⁺/CD₈⁺）、结肠镜及病理检查评分、中医症状（腹痛、腹泻、黏液脓血便、里急后重评分及总积分）评分、治疗有效率及复发率。结果 治疗后,研究组白细胞介素-6、肿瘤坏死因子-α、超敏C反应蛋白水平低于对照组,白细胞介素-10高于对照组,差异具有统计学意义（P<0.05）;研究组CD₃⁺、CD₈⁺水平明显低于对照组,CD₄⁺与CD₄⁺/CD₈⁺明显高于对照组（P<0.05）;研究组结肠镜、病理检查评分均明显优于对照组（P<0.05）;2组治疗后中医症状均明显好转,研究组治疗后中医症状评分均明显低于对照组（P<0.05）;研究组治疗总有效率明显高于对照组（P<0.05）;治疗后随访6个月,研究组复发率明显低于对照组（P<0.05）。结论 中药多途径治疗溃疡性结肠炎的临床疗效确切,有利于降低炎症反应,提升肠道黏膜免疫功能,促进溃疡愈合,改善中医症状,安全性高,复发率低,值得临床应用。     

YAP和Caspase在恶性肿瘤中的作用机制及其关联的研究进展

正常情况下细胞增殖与凋亡之间保持着动态平衡,维持着机体的生理状态,细胞异常增殖或凋亡抑制时细胞过度增殖组织异常生长导致肿瘤的发生。Hippo通路是最近发现的一条细胞信号传导通路,主要功能有调控器官体积、保持细胞增殖凋亡平衡、细胞接触性抑制的调节等。YAP是Hippo通路上的重要因子,YAP表达异常可导致Hippo通路不能正常发挥作用而细胞异常增殖导致肿瘤的发生。Caspase是与细胞凋亡密切相关的蛋白水解酶家族,一般以酶原形式存在,在凋亡信号的作用下激活启动型Caspase引发Caspase级联反应导致细胞凋亡,其功能异常导致各种相关疾病的发生。本文将介绍Hippo通路的主要组成及其作用机制,YAP和Caspase基因的作用机制及其与肿瘤发生发展的相关性。     

羟基磷灰石骨修复材料

该文就当前骨修复材料研究的热点——羟基磷灰石生物活性陶瓷的晶体结构和组成、力学性质、生物降解性和粉末的制备等方面作了较为详细的阐述，并对其发展趋势进行了探讨。认为羟基磷灰石是一种很有前途的陶瓷人工骨置换材料。     

姚芷龄从肝风下迫辨治女子阴道坠胀验案2则

目的阴道坠胀临床多从气虚不能托举脏器论治,有时并不能达到很好效果,该文从肝风下迫入手,可以对其他临床医生辨治类似病证起到一定的启迪作用。方法列举姚芷龄从肝风下迫辨治妇女阴道坠痛验案2则,以阐释对于妇女阴道坠胀从肝风下迫理论辨治的疗效。结果平肝息风法治疗阴道坠胀临床疗效甚佳。结论从肝风下迫辨治妇女阴道坠痛具有较大临床意义,除可考虑气虚不能托举脏器外,也应从肝风下迫脏腑的角度考虑病情。     

SD大鼠脏器重量及脏器系数正常参考值研究

农药安全性评价中,脏器重量及脏器系数是亚慢性毒性试验中检测受试物毒作用的重要指标.我们分析了2年多来11批亚慢性试验（90 d）中阴性对照组264只SD大鼠（雌雄各半）的体重和脏器重量的测定结果,求出脏器重量及脏器系数的正常参考值,供安全性评价时参考.