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61.
High-Throughput Generation of P. falciparum Functional Molecules by Recombinational Cloning 总被引:2,自引:0,他引:2
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Joo Carlos Aguiar Joshua LaBaer Peter L. Blair Victoria Y. Shamailova Malvika Koundinya Joshua A. Russell Fengying Huang Wenhong Mar Robert M. Anthony Adam Witney Sonia R. Caruana Leonardo Brizuela John B. Sacci Jr. Stephen L. Hoffman Daniel J. Carucci 《Genome research》2004,14(10B):2076-2082
Large-scale functional genomics studies for malaria vaccine and drug development will depend on the generation of molecular tools to study protein expression. We examined the feasibility of a high-throughput cloning approach using the Gateway system to create a large set of expression clones encoding Plasmodium falciparum single-exon genes. Master clones and their ORFs were transferred en masse to multiple expression vectors. Target genes (n = 303) were selected using specific sets of criteria, including stage expression and secondary structure. Upon screening four colonies per capture reaction, we achieved 84% cloning efficiency. The genes were subcloned in parallel into three expression vectors: a DNA vaccine vector and two protein expression vectors. These transfers yielded a 100% success rate without any observed recombination based on single colony screening. The functional expression of 95 genes was evaluated in mice with DNA vaccine constructs to generate antibody against various stages of the parasite. From these, 19 induced antibody titers against the erythrocytic stages and three against sporozoite stages. We have overcome the potential limitation of producing large P. falciparum clone sets in multiple expression vectors. This approach represents a powerful technique for the production of molecular reagents for genome-wide functional analysis of the P. falciparum genome and will provide for a resource for the malaria resource community distributed through public repositories. 相似文献
62.
František Moravec Sonia Ternengo Céline Levron 《Acta parasitologica / Witold Stefański Institute of Parasitology, Warszawa, Poland》2006,51(2):111-118
Gravid or subgravid females of the following three species belonging to the nematode genus Philometra were collected from marine perciform fishes of the Bonifacio Strait Marine Reserve, Corsica, France: Philometra justinei sp. nov. (prevalence 1.5%, intensity 11) and Philometra sp. (prevalence 3%, intensity 2) from the abdominal cavity and gonads, respectively, of the striped red mullet Mullus surmuletus (Mullidae), and Philometra serranellicabrillae Janiszewska, 1949 (prevalence 27%, intensity 2–6) from the gonads of the comber Serranus cabrilla (Serranidae). The newly described species, P. justinei, is mainly characterized by the absence of an anterior bulbous inflation of the oesophagus, body length of the gravid female
(196 mm), conspicuously large amphids, number and distribution of cephalic papillae, and the morphology of larvae. It is the
second known species of Philometra parasitizing fishes of the family Mullidae and the ninth species of this genus reported from marine fishes of the Mediterranean
region. P. serranellicabrillae, studied for the first time by SEM, is redescribed. 相似文献
63.
Morand-Joubert L Marcellin F Launay O Guiramand-Hugon S Gérard L Yeni P Aboulker JP;Agence Nationale de Recherches sur le SIDA Study Group 《Journal of acquired immune deficiency syndromes (1999)》2005,38(3):268-276
Cellular HIV-1 DNA level was sequentially measured by quantitative polymerase chain reaction in 141 patients not previously treated with highly active antiretroviral therapy (HAART), who were enrolled in a 72-week randomized trial (ANRS 081 "Trianon") comparing 2 regimens, including 3 drugs from 2 classes (indinavir + stavudine + lamivudine, group 1) or 3 classes (indinavir + stavudine + nevirapine, group 2). The median decrease from baseline to week 72 in cellular HIV-1 DNA level was not significantly different between the 2 groups (0.54 and 0.45 log10 copies/10 peripheral blood mononuclear cells [PBMCs] in groups 1 and 2, respectively), whereas a higher proportion of patients maintained a plasma HIV-1 RNA level less than 20 copies/mL at week 72 in group 1 than in group 2 (79% and 52%; P = 0.0009). Furthermore, the difference in cellular HIV-1 DNA decrease from baseline to week 72 between patients who achieved a plasma HIV-1 RNA level less than 20 copies/mL at week 72 and those who did not was not statistically significant (0.54 and 0.45 log10 copies/10 PBMCs, respectively; P = 0.14). The decay in cellular HIV-1 DNA from baseline to week 72 was higher in antiretroviral-naive patients than in pretreated patients (0.55 and 0.23 log10 copies/10 PBMCs, respectively; P = 0.0008). The cellular HIV-1 DNA level change under therapy was best fitted to a 2-phase decay model with a junction point at week 16, from which its half-life was estimated at 18 weeks during the initial phase and at 104 weeks thereafter. In conclusion, the changes under therapy in cellular HIV-1 DNA level, which were mostly coincident to those of plasma HIV-1 RNA, did not add significant information to the comparison of the viral efficacy of the 2 studied regimens. 相似文献
64.
STUDY OBJECTIVES: The goal of this study was to examine the natural history of periodic limb movements in sleep (PLMS) in older adults. DESIGN: Prospective, longitudinal design. SETTING: San Diego area. PARTICIPANTS: Community-dwelling older adults. INTERVENTIONS: NA MEASUREMENTS AND RESULTS: Subjects participated in follow-up visits every few years. Sleep disorders were assessed at each visit using sleep questionnaires and overnight sleep recordings. Changes in PLMS over time were examined as well as variables that were associated with change. Average PLMS did not change over time, although there was considerable variability within the sample. In women, changes in PLMS were associated with changes in sleep-disordered breathing. This relationship was not found in men. CONCLUSIONS: Although the prevalence of PLMS is high for older adults, the severity of the disorder does not progressively worsen with increasing age. 相似文献
65.
Bone-specific antibodies in sera from patients with celiac disease: characterization and implications in osteoporosis 总被引:5,自引:0,他引:5
Sugai E Cherñavsky A Pedreira S Smecuol E Vazquez H Niveloni S Mazure R Mauriro E Rabinovich GA Bai JC 《Journal of clinical immunology》2002,22(6):353-362
Osteopenia and osteoporosis are well-known complications detected in celiac disease patients with still obscure pathogenesis. In the present study we investigated the presence of circulating anti-bone autoantibodies in patients with celiac disease and explored their role in the associated bone disease. We evaluated serum samples from 33 patients at the time of diagnosis and from 20 of them after treatment. Sera from patients with inflammatory bowel disease (n = 9), nonceliac osteoporotic (n = 18), and healthy individuals (n = 10) were used as controls. The presence of IgA specific anti-bone antibodies was first investigated using indirect immunofluorescence on cryosections of fetal rat tibia (20-day pregnancy). Furthermore, samples were homogenized and total tissue extracts were subjected to Western blot analysis to confirm immunoreactivity. At diagnosis, sera from 51.5% (17/33) of celiac patients had antibodies that recognized antigenic structures in chondrocytes and the extracellular matrix along mature cartilage, bone interface, and perichondrium of fetal rat bone. Among controls, only two osteoporotic patients showed very low titles of anti-bone autoantibodies. The immunostaining was localized in areas where an active mineralization process occurred and was similar to the distribution of the native bone tissue transglutaminase. The frequency of patients with positive baseline titers of anti-bone antibodies diminished significantly after treatment (P = 0.048). Western blot assays confirmed the presence of autoantibodies in sera from patients with a positive immunofluorescence staining. Autoantibodies recognized a major protein band on tissue extracts with a molecular weight of 77–80 kDa, which could be displaced when sera were preadsorbed with human recombinant tissue transglutaminase. We provide original evidence that patients with celiac disease have IgA-type circulating autoantibodies against intra- and extracellular structures of fetal rat tibia. Our findings suggest that these antibodies recognize bone tissue transglutaminase as the autoantigen, and based on the localization of the immunoreactivity we speculate that they might have an active role in the pathophysiology of celiac disease-associated bone complications. 相似文献
66.
Heterogeneous pattern of chromosomal breakpoints involving the MYC locus in multiple myeloma 总被引:2,自引:0,他引:2
Fabris S Storlazzi CT Baldini L Nobili L Lombardi L Maiolo AT Rocchi M Neri A 《Genes, chromosomes & cancer》2003,37(3):261-269
Chromosomal rearrangements of the MYC locus, which often involve the IG loci, are recurrent events in multiple myeloma (MM) and plasma cell leukemia (PCL). We used dual-color fluorescence in situ hybridization (FISH) to characterize the breakpoint locations of chromosomal translocations/rearrangements involving the MYC locus at 8q24 found in a panel of 14 MM cell lines and 70 primary tumors (66 MM and 4 PCL). MYC locus alterations were observed in 21 cases: MYC/IG (mainly IGH@) fusions in 11 cell lines and three patients (2 MM and 1 PCL), and extra signals and/or abnormal MYC localizations in seven patients (5 MM and 2 PCL). Fourteen of these cases were investigated by FISH analyses by use of a panel of BAC clones covering about 6 Mb encompassing the MYC locus. The breakpoints were localized in a region 100-250 kb centromeric to MYC in four cases, a region 500-800 kb telomeric to the gene in four cases, and regions > or = 2 Mb centromeric or telomeric to MYC in five cases. Two different breakpoints were detected in the KMS-18 cell line, whereas the insertion of a MYC allele was found in a complex t(16;22) chromosomal translocation in the RPMI8226 cell line. Our data document a relatively high dispersion of 8q24 breakpoints in MM. 相似文献
67.
Maria do Carmo Debur Juliano Bordignon Claudia Nunes Duarte dos Santos Luine Rosele Vidal Meri Bordignon Nogueira Sérgio Monteiro de Almeida Sonia Mara Raboni 《Journal of clinical virology》2007,39(1):59-62
BACKGROUND: Human metapneumovirus (hMPV) has been described as an etiologic agent of acute respiratory infections (ARI), mainly in pediatric patients. Viral isolation is difficult and has low sensitivity, and consequently RT-PCR assays are currently used for detection. OBJECTIVES: Detect hMPV in ARI in hospitalized children in Southern Brazil; standardize a RT-PCR for routine hMPV diagnosis; validate a positive control for molecular tests; and perform phylogenetics analyses. STUDY DESIGN: Nasopharyngeal aspirates (NPA) from 156 hospitalized children were studied. A conserved region of the nucleoprotein gene was cloned, characterized and used to standardize an RT-PCR assay. Phylogenetic analyses were performed. Clinical data were obtained from medical records. RESULTS: hMPV was detected in 6.4% of the samples. Dyspnea and wheezing were frequently reported symptoms and the most common diagnoses were bronchiolitis, acute respiratory insufficiency or laryngotracheobronchitis. Nucleotide sequence alignment revealed 97.7% identity with genotype A1 of hMPV. The detection limit of hMPV genomes by RT-PCR in clinical samples was 180 copies/microL. CONCLUSION: This is the first report of the detection and genetic characterization of hMPV infections in children with lower ARI in Southern Brazil. 相似文献
68.
Egede Leonard E. Walker Rebekah J. Dawson Aprill Z. Zosel Amy Bhandari Sanjay Nagavally Sneha Martin Ian Frank Michael 《Quality of life research》2022,31(8):2387-2396
Quality of Life Research - This study aimed to investigate changes over time in quality of life, perceived stress, and serious psychological distress for individuals diagnosed with COVID-19 in an... 相似文献
69.
Marco Crocco Angela Calvi Paolo Gandullia Federica Malerba Anthea Mariani Sonia Di Profio Barbara Tappino Stefano Bonassi 《Nutrients》2021,13(2)
We aimed to assess Health-Related Quality of Life (HRQoL) of Italian children and their parents with coeliac disease (CD) using the Coeliac Disease Dutch Questionnaire (CDDUX). The CDDUX underwent a cross-cultural adaptation in a multi-step process, according to international guidelines. A total of 224 children aged between 8–18 years and their parents were prospectively recruited. Cronbach α coefficient was determined as a measure of internal consistency of the questionnaire and inter-children/parent reliability by intraclass correlation coefficient. Univariate and bivariate regression models were used to evaluate correlations between clinical variables and children and parents subclasses of CDDUX and overall mean Paediatric Quality of Life Inventory (PedsQL). The Italian CDDUX proved to be valid and reliable, mean CDDUX total score revealing a neutral evaluation of the quality of life in children 52.6 ± 17.2 and parents 49.5 ± 17.9 (p = 0.07) with strong correlation with PedsQL. The only clinical variable which appeared to affect significantly quality of life both in children and parents was the lower age. A comparison with our results showed remarkable differences in the HRQoL of populations of various nationalities. The Italian version of the CDDUX questionnaire is a simple and reliable tool for assessing the HRQoL in children and adolescents with CD. 相似文献
70.
Asher Y. Rosinger Samantha M. Olson Sascha R. Ellington Janice Perez-Padilla Regina M. Simeone Caitlin S. Pedati Betsy A. Schroeder Gilberto A. Santiago Freddy A. Medina Jorge L. Muoz-Jordn Laura E. Adams Romeo R. Galang Miguel Valencia-Prado Sonia Bakkour Candimar Coln Mary Goodwin Dana Meaney-Delman Jennifer S. Read Lyle R. Petersen Denise J. Jamieson Carmen C. Deseda Margaret A. Honein Brenda Rivera-García Carrie K. Shapiro-Mendoza 《Emerging infectious diseases》2021,27(5):1505
We evaluated nucleic acid amplification testing (NAAT) for Zika virus on whole-blood specimens compared with NAAT on serum and urine specimens among asymptomatic pregnant women during the 2015–2016 Puerto Rico Zika outbreak. Using NAAT, more infections were detected in serum and urine than in whole blood specimens. 相似文献