Über das Verhalten von Herzglykosiden in der Niere als Ursache ihrer unterschiedlichen diuretischen Aktivität

Ohne Zusammenfassung     

Der Nährwert des Colostrums

Ohne ZusammenfassungErschien gleichzeitig in der Festschrift für Heubner.     

Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisation

The comet assay is widely used to measure DNA damage and repair in basic research, genotoxicity testing and human biomonitoring. The conventional format has 1 or 2 gels on a microscope slide, 1 sample per slide. To increase throughput, we have designed and tested a system with 12 smaller gels on one slide, allowing incubation of individual gels with different reagents or enzymes. Thus several times more samples can be analysed with one electrophoresis run, and fewer cells and smaller volumes of test solutions are required. Applications of the modified method include treatment with genotoxic agents at different concentrations; simultaneous analysis of different lesions using a range of enzymes; analysis of cell extracts for DNA repair activity; and fluorescent in situ hybridisation (FISH) to comet DNA with specific labelled probes.     

Effect of preservation media on proliferation and collagen biosynthesis of periodontal ligament fibroblasts

Abstract The viability of periodontal ligament (PDL) cells, which are responsible for the production of ligament proteins, is essential for clinical healing of replanted exarticulated teeth. Cultured human PDL fibroblast-like cells were used to study the effects of various transport media on cell proliferation, collagen synthesis and total protein synthesis. The PDL cells were obtained from intact premolars of a healthy young male patient by trypsinization and scraping the PDL tissue. Proliferation of the cells, the activity of prolyl 4-hydroxylase, total protein and collagen synthesis were studied after 60 min incubation in Dulbecco's Modified Eagle's Medium (DMEM), milk, saliva and tap water. Proliferation capacity was also investigated after prolonged incubation in milk. The results indicated that milk and saliva were superior to water in maintaining these cell functions, but as the saliva used in this study does not resemble that in the clinical situation, we recommend milk as a temporary storage medium for exarticulated teeth before replantation.     

Osteoclasts and Biomaterials

Abstract There is a growing market of biomaterials for orthopedic applications. As soon as these materials are surgically introduced into the constantly remodeling bone of the patient, they start to interact with the local cells: osteoblasts and osteoclasts. At the first glance, the bone building osteoblasts seem to be the more important cells for osseointegration of implants. However, it is mainly the bone resorbing action of osteclasts that determines the longevity of the implant. In this paper, we give a short overview over the current understanding of osteoclast biology; we review the interaction between biomaterials, biomaterial particles and osteoclasts, and the effects of treatment with antiosteoclastic agents like bisphosphonates on biomaterial implant healing.     

Neue und alte Antikoagulanzien im Blickpunkt

In the surgery and therapy of trauma patients, the decision for optimal thromboprophylaxis is often very difficult, although important. In the last 2 years, new anti-thrombotic agents have been developed and are now used in Germany for thromboprophylaxis in patients with hip or knee replacement surgery. This article reviews old (heparinoids and vitamin K-antagonists) and new (fondaparinux and ximelagatran) anticoagulants in perioperative settings of trauma surgery and discusses alternative anticoagulation in patients on therapy with vitamin K-antagonists.     

A study of ten small supernumerary (marker) chromosomes identified by fluorescence in situ hybridization (FISH)

In seven cases additional minute chromosomes studied by FISH were identified as no. 3, 11, 15, 18, 21 and X. Findings were unexpected except for partial trisomy 21 in an adolescent with minor features of Down's syndrome. Moreover, an i(18p) in a mentally retarded dysmorphic child and an idic(15) in a child with Fallot tetralogy was confirmed. In a child with r(21), a supernumerary marker was shown to be derived from no. 21, while in the mother an additional marker idic(22) was noted.     

Coping in longitudinal perspective: Findings from the bonn longitudinal study on aging

Within the Bonn Longitudinal Study on Aging (BOLSA) 221 women and men, born between 1890–95 and between 1900–05, were tested and interviewed in the year 1965 for the first time. Survivors were followed at seven measurement points until 1980. One of three lengthy interviews was related to the topic of stress and coping. Information on perceived stresses in the areas of housing, income, family and health were rated. Furthermore, responses to these different stress areas were analyzed by an empirically developed classification system. Findings point to a complex system of response patterns even in the group of very old participants. These response patterns were different for the four areas of stress, such as family or health etc. Response patterns related to the same problem area remained consistent over the 15-year span. Cluster analyses point to situation specific as well as person specific response patterns.This paper was originally presented at the XIV International Congress of Gerontology, Acapulco, Mexico, 18–23 June 1989.