全文获取类型
收费全文 | 596篇 |
免费 | 32篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 14篇 |
妇产科学 | 8篇 |
基础医学 | 225篇 |
口腔科学 | 6篇 |
临床医学 | 45篇 |
内科学 | 137篇 |
皮肤病学 | 12篇 |
神经病学 | 47篇 |
特种医学 | 5篇 |
外科学 | 50篇 |
综合类 | 4篇 |
预防医学 | 31篇 |
眼科学 | 5篇 |
药学 | 23篇 |
肿瘤学 | 15篇 |
出版年
2022年 | 12篇 |
2021年 | 11篇 |
2020年 | 12篇 |
2018年 | 9篇 |
2017年 | 8篇 |
2016年 | 8篇 |
2015年 | 8篇 |
2014年 | 12篇 |
2013年 | 19篇 |
2012年 | 31篇 |
2011年 | 30篇 |
2010年 | 17篇 |
2009年 | 22篇 |
2008年 | 26篇 |
2007年 | 24篇 |
2006年 | 20篇 |
2005年 | 21篇 |
2004年 | 26篇 |
2003年 | 24篇 |
2002年 | 19篇 |
2001年 | 20篇 |
2000年 | 16篇 |
1999年 | 10篇 |
1998年 | 4篇 |
1997年 | 6篇 |
1996年 | 5篇 |
1993年 | 5篇 |
1992年 | 9篇 |
1991年 | 12篇 |
1990年 | 8篇 |
1989年 | 7篇 |
1988年 | 9篇 |
1987年 | 8篇 |
1985年 | 9篇 |
1984年 | 10篇 |
1983年 | 4篇 |
1981年 | 8篇 |
1980年 | 7篇 |
1979年 | 11篇 |
1978年 | 12篇 |
1977年 | 7篇 |
1975年 | 4篇 |
1974年 | 6篇 |
1972年 | 13篇 |
1971年 | 6篇 |
1970年 | 3篇 |
1969年 | 6篇 |
1968年 | 5篇 |
1966年 | 3篇 |
1963年 | 3篇 |
排序方式: 共有628条查询结果,搜索用时 15 毫秒
21.
Anastazija Hvala Dušan Ferluga Tomaž Rott Tatjana Kobenter Mira Koselj-Kajtna Staša Kaplan-Pavlovčič 《Ultrastructural pathology》2013,37(4):295-299
Knowledge about the normal structure and pathology of interstitial capillary is limited. Splitting and multilayering of the basal membrane (BM), as a marker of chronic rejection, has been published in association with transplant glomerulopathy. The authors investigated the ultrastructural features of the interstitial capillary basal membrane in normal (15 biopsies) and in transplanted kidneys (27 biopsies from 21 patients), expressing transplant glomerulopathy (8 biopsies from 6 patients), acute tubulo-interstitial rejection (9 biopsies from 6 patients), and recurrent or de novo glomerulonephritis (10 biopsies from 8 patients). All biopsies were fixed in 1%OsO 4, embedded in Epon, and examined by electron microscope. Measurements of the interstitial capillary BM were made. The BM of interstitial capillary of intact kidney was a homogenous continuous structure, 88 nm in width on average. Thickening with diffuse multilayering of BM was most intensive in patients with transplant glomerulopathy, and much less intensive in patients with acute tubulointerstitial rejection and in patients with recurrent or de novo glomerulonephritis. These findings may provide the first information about the morphology of the normal basal lamina of interstitial capillary and support the diagnostic value of interstitial capillary changes in chronic rejection. 相似文献
22.
23.
Jari J. Hirvonen Silja Mentula Suvi-Sirkku Kaukoranta 《Journal of clinical microbiology》2013,51(9):2908-2912
We evaluated a new automated homogeneous PCR assay to detect toxigenic Clostridium difficile, the GenomEra C. difficile assay (Abacus Diagnostica, Finland), with 310 diarrheal stool specimens and with a collection of 33 known clostridial and nonclostridial isolates. Results were compared with toxigenic culture results, with discrepancies being resolved by the GeneXpert C. difficile PCR assay (Cepheid). Among the 80 toxigenic culture-positive or GeneXpert C. difficile assay-positive fecal specimens, 79 were also positive with the GenomEra C. difficile assay. Additionally, one specimen was positive with the GenomEra assay but negative with the confirmatory methods. Thus, the sensitivity and specificity were 98.8% and 99.6%, respectively. With the culture collection, no false-positive or -negative results were observed. The analytical sensitivity of the GenomEra C. difficile assay was approximately 5 CFU per PCR test. The short hands-on (<5 min for 1 to 4 samples) and total turnaround (<1 h) times, together with the high positive and negative predictive values (98.8% and 99.6%, respectively), make the GenomEra C. difficile assay an excellent option for toxigenic C. difficile detection in fecal specimens. 相似文献
24.
Ana Paula Costa de Aguiar Caroline de Oliveira Silveira Mari Aline Todero Winck Marilise Brittes Rott 《Acta parasitologica / Witold Stefański Institute of Parasitology, Warszawa, Poland》2013,58(3):304-308
The present study investigated the susceptibility of Acanthamoeba spp. trophozoites to two multipurpose systems for cleaning and maintenance of contact lenses. Three strains of trophozoites from the ATCC (A. castellani T4, A. castellani Neff, and A. polyphaga) and two Acanthamoeba isolates obtained from swimming pools (PT5 and PO1) were placed in monoxenic culture. To test their survival in cleaning solutions for contact lenses, the trophozoites were exposed for 4 and 24 h to two multipurpose solutions (A and B), and were then inoculated into a new monoxenic culture. Amoebic growth on the plates was observed after 72 h of incubation. Trophozoites from all three ATCC strains and one isolate from a swimming pool (PO1) grew in all plates after 4 h of exposure to solutions A and B. After 24 h, the ATCC strains and the PO1 isolate showed growth in most of the plates treated. Only the PT5 isolate showed susceptibility to both solutions over the time intervals tested. The two solutions were not completely effective against most strains and isolates over the time intervals tested. These results are important, since species of Acanthamoeba are widely distributed in the environment and are potential agents of eye pathologies. 相似文献
25.
Verena Tenten Sylvia Menzel Uta Kunter Eva-Maria Sicking Claudia R. C. van Roeyen Silja K. Sanden Michaela Kaldenbach Peter Boor Astrid Fuss Sandra Uhlig Regina Lanzmich Brigith Willemsen Henry Dijkman Martin Grepl Klemens Wild Wilhelm Kriz Bart Smeets Jürgen Floege Marcus J. Moeller 《Journal of the American Society of Nephrology : JASN》2013,24(12):1966-1980
26.
Silja Vocks Joachim Kosfelder Maike Wucherer Alexandra Wächter 《Psychotherapy research》2013,23(4):412-419
Abstract The aim of the present study was to assess whether habitual body avoidance and body-checking behavior influences the decrease of negative emotions during body exposure. Twenty-one eating-disordered female participants completed the Body Image Avoidance Questionnaire and the Body Checking Questionnaire. On another day, a 40-min body exposure session was conducted under standardized conditions. Every 10 min, negative emotions were assessed. It was shown that the extent of decrease in negative emotions during the body exposure session could be predicted by a lower degree of body checking. Results indicate that habitual checking behavior seems to negatively influence the effect of body exposure. Therefore, an adaptation of body exposure to patients with a higher degree of body-checking behavior might be promising. 相似文献
27.
Patrick Smith Ph.D. Martti T. Tuomisto Ph.D. James Blumenthal Ph.D. Andrew Sherwood Ph.D. Lauri Parkkinen MS Mika Kähönen MD Ilkka Pörsti MD Silja Majahalme MD Väinö Turjanmaa MD 《Annals of behavioral medicine》2013,45(1):99-109
Background
Psychosocial factors have been associated with cardiovascular outcomes, but few studies have examined the association between psychosocial function and natriuretic peptides.Purpose
The purpose of this study is to determine the predictive value of hostility, anger, and social support in relation to atrial natriuretic peptide (ANP), a marker of vascular health, among middle-aged men.Methods
One hundred twenty-one men (mean age?=?39.8 years, SD?=?4.1) underwent assessments of ANP and completed the Cook–Medley Hostility Scale, the Spielberger State–Trait Anger Scale, and the Interview Schedule for Social Interaction.Results
Higher levels of hostility (β?=?0.22 [95 % CI 0.04, 0.40], P?=?0.032) and trait anger (β?=?0.18 [95 % CI 0.01, 0.37], P?=?0.044) were associated with greater ANP levels. In contrast, higher perceived social support was also associated with lower ANP levels, (β?=??0.19 [95 % CI ?0.05, ?0.41], P?=?0.010).Conclusions
Psychosocial factors, including hostility, anger, and social support, are associated with varying ANP levels among middle-aged men, independent of cardiovascular and behavioral risk factors. 相似文献28.
Heterologous production of albicidin: a promising approach to overproducing and characterizing this potent inhibitor of DNA gyrase
下载免费PDF全文
![点击此处可从《Antimicrobial agents and chemotherapy》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Vivien E Pitorre D Cociancich S Pieretti I Gabriel DW Rott PC Royer M 《Antimicrobial agents and chemotherapy》2007,51(4):1549-1552
The phytotoxin and polyketide antibiotic albicidin produced by Xanthomonas albilineans is a highly potent DNA gyrase inhibitor. Low yields of albicidin production have slowed studies of its chemical structure. Heterologous expression of albicidin biosynthetic genes in X. axonopodis pv. vesicatoria resulted in a sixfold increase in albicidin production, offering promising strategies for engineering overproduction. 相似文献
29.
Jean H. Daugrois Denis Filloux Charlotte Julian Lisa Claude Romain Ferdinand Emmanuel Fernandez Hugo Fontes Philippe C. Rott Philippe Roumagnac 《Viruses》2021,13(5)
Visacane is a sugarcane quarantine station located in the South of France, far away from sugarcane growing areas. Visacane imports up to 100 sugarcane varieties per year, using safe control and confinement measures of plants and their wastes to prevent any risk of pathogen spread outside of the facilities. Viruses hosted by the imported material are either known or unknown to cause disease in cultivated sugarcane. Poaceae viruses occurring in plants surrounding the quarantine glasshouse are currently unknown. These viruses could be considered as a source of new sugarcane infections and potentially cause new sugarcane diseases in cases of confinement barrier failure. The aim of this study was to compare the plant virome inside and outside of the quarantine station to identify potential confinement failures and risks of cross infections. Leaves from quarantined sugarcane varieties and from wild Poaceae growing near the quarantine were collected and processed by a metagenomics approach based on virion-associated nucleic acids extraction and library preparation for Illumina sequencing. While viruses belonging to the same virus genus or family were identified in the sugarcane quarantine and its surroundings, no virus species was detected in both environments. Based on the data obtained in this study, no virus movement between quarantined sugarcane and nearby grassland has occurred so far, and the confinement procedures of Visacane appear to be properly implemented. 相似文献
30.
Arijit Biswas Vytautas Ivaskevicius Anne Thomas Michael Varvenne Brigitte Brand Hannelore Rott Iris Haussels Heiko Ruehl Ute Scholz Robert Klamroth Johannes Oldenburg 《Annals of hematology》2014,93(10):1665-1676
Mild FXIII deficiency is an under-diagnosed disorder because the carriers of this deficiency are often asymptomatic and reveal a phenotype only under special circumstances like surgery or induced trauma. Mutational reports from this type of deficiency have been rare. In this study, we present the phenotypic and genotypic data of nine patients showing mild FXIII-A deficiency caused by eight novel heterozygous missense mutations (Pro166Leu, Arg171Gln, His342Tyr, Gln415Arg, Leu529Pro, Gln601Lys, Arg703Gln and Arg715Gly) in the F13A1 gene. None of these variants were seen in 200 healthy controls. In silico structural analysis of the local wild-type protein structures (activated and non-activated) from X-ray crystallographic models downloaded from the protein databank identified potential structural/functional effects for the identified mutations. The missense mutations in the core domain are suggested to be directly influencing the catalytic triad. Mutations on other domains might influence other critical factors such as activation peptide cleavage or the barrel domain integrity. In vitro expression and subsequent biochemical studies in the future will be able to confirm the pathophysiological mechanisms proposed for the mutations in this article. 相似文献