全文获取类型
收费全文 | 3771篇 |
免费 | 182篇 |
国内免费 | 31篇 |
专业分类
耳鼻咽喉 | 14篇 |
儿科学 | 84篇 |
妇产科学 | 34篇 |
基础医学 | 901篇 |
口腔科学 | 49篇 |
临床医学 | 177篇 |
内科学 | 760篇 |
皮肤病学 | 144篇 |
神经病学 | 359篇 |
特种医学 | 108篇 |
外科学 | 521篇 |
综合类 | 10篇 |
预防医学 | 68篇 |
眼科学 | 127篇 |
药学 | 216篇 |
中国医学 | 7篇 |
肿瘤学 | 405篇 |
出版年
2023年 | 23篇 |
2022年 | 26篇 |
2021年 | 66篇 |
2020年 | 40篇 |
2019年 | 72篇 |
2018年 | 67篇 |
2017年 | 54篇 |
2016年 | 60篇 |
2015年 | 67篇 |
2014年 | 96篇 |
2013年 | 121篇 |
2012年 | 166篇 |
2011年 | 188篇 |
2010年 | 96篇 |
2009年 | 96篇 |
2008年 | 224篇 |
2007年 | 207篇 |
2006年 | 230篇 |
2005年 | 291篇 |
2004年 | 281篇 |
2003年 | 290篇 |
2002年 | 275篇 |
2001年 | 49篇 |
2000年 | 30篇 |
1999年 | 62篇 |
1998年 | 76篇 |
1997年 | 81篇 |
1996年 | 50篇 |
1995年 | 61篇 |
1994年 | 50篇 |
1993年 | 54篇 |
1992年 | 43篇 |
1991年 | 26篇 |
1990年 | 27篇 |
1989年 | 37篇 |
1988年 | 21篇 |
1987年 | 19篇 |
1986年 | 31篇 |
1985年 | 20篇 |
1984年 | 25篇 |
1983年 | 22篇 |
1982年 | 8篇 |
1981年 | 12篇 |
1980年 | 23篇 |
1979年 | 6篇 |
1978年 | 16篇 |
1977年 | 10篇 |
1976年 | 10篇 |
1975年 | 7篇 |
1969年 | 7篇 |
排序方式: 共有3984条查询结果,搜索用时 0 毫秒
61.
Hyperglycemia increases oxidative stress in various tissues and leads to diabetic cardiovascular complication. Dyslipidemia,
such as an increase in oxidized low-density lipoprotein (LDL), is well recognized in diabetic patients with hyperglycemia.
However, the mechanism by which hyperglycemia causes the increased LDL oxidation remains unclear. Albumin is the most abundant
protein in the circulation, and can function as an antioxidant. Therefore, we examined whether glycoxidative modification
inhibits the antioxidant activity of albumin to LDL oxidation and clarified the mechanism by which this modification may suppress
its antioxidant activity. Human serum albumin (HSA) was incubated in phosphate-buffered saline with and without glucose at
37°C for up to 8 weeks under aerobic conditions (referred to as glycoxidation (goHSA) and oxidation (oHSA), respectively).
Metal chelator-treated, nonoxidative HSA (chHSA) and freshly prepared HSA (fHSA) were used as controls. N
ε-(carboxymethyl)lysine (CML), a glycoxidative product, was determined by enzyme-linked immunosorbent assay. Oxidation was
estimated by measuring the thiols of the HSA molecule. Copper-mediated oxidation of LDL was conducted in the presence or absence
of modified HSAs at 37°C for 6 days. Malondialdehyde and negative charge of LDL were measured. To clarify the mechanism of
reduced antioxidant activity of HSA, we examined firstly the binding activity of modified HSAs to copper, and secondly the
effects of free radical scavengers on the formation of malondialdehyde. CML was formed in goHSA in a time- and concentration-dependent
manner. Both goHSA and oHSA significantly decreased the contents of free thiol groups compared to ch- and fHSAs. The antioxidant
activity of goHSA to LDL oxidation was the lowest among various modified HSAs. The oHSA showed a moderate decrease in antioxidant
activity. The binding activity of go- and oHSAs to copper was lower than that of ch- and fHSAs. The formation of MDA from
LDL oxidation in the presence of goHSA was completely inhibited by Tiron (1,2-dihydroxy-3,5-benzenedisulfonic acid) and superoxide
dismutase. In contrast, catalase and mannitol had no effect. Our results indicate that in vitro glycoxidation of HSA induced
a marked loss of antioxidant activity of this molecule to copper-mediated oxidation of LDL, which may be caused by the generation
of superoxide.
Received: December 17, 2001 / Accepted: June 28, 2002
Acknowledgments The authors thank Drs. Ryoji Nagai and Seikoh Horiuchi (Department of Biochemistry, Kumamoto University School of Medicine,
Kumamoto, Japan) and Drs. Hiroyuki Itabe and Tatsuya Takano (Department of Microbiology and Molecular Pathology, Faculty of
Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa, Japan) for kindly supplying antibodies. We also thank Associate
Professor Takeo Yamaguchi (Department of Chemistry, Faculty of Science, Fukuoka University) for the ESR experiment and Miss
Satoko Nagano for her excellent technical assistance. This work was supported by a Grant-in-Aid from the Ministry of Education,
Science, Sports and Culture of Japan (No. 14570171) and in part by funds from the Central Research Institute of Fukuoka University
(No. 016004).
Correspondence to N. Sakata 相似文献
62.
Takemura N Kono K Tadokoro K Shinbo G Ito I Abe C Matsuhashi N Iemura T Nishikimi T Horinaka S Matsuoka H 《Journal of cardiology》2008,51(3):205-209
We describe a 59-year-old woman with sick sinus syndrome (SSS) and arrhythmogenic right ventricular cardiomyopathy (ARVC). Diagnosis of SSS was made because she had frequent episodes of sinus arrest with prolonged ventricular asystole. Cardiac images showed a dilated right atrium (RA) and a right ventricle (RV). Electroanatomical mapping of the RA showed extensive scarring with no recordable electrical potentials. Although she had frequent premature ventricular contractions, neither spontaneous ventricular tachycardia (VT) nor induced VT was observed. Microscopic examination of the RV indicated fibrofatty myocardium. Atrial arrhythmias associated with SSS may be the cause of symptoms in some cases of ARVC. 相似文献
63.
64.
Higashiura Masaki Ohya Masaki Tanaka Yusuke Yano Takuro Yamamoto Shuto Mima Toru Negi Shigeo Shigematsu Takashi 《International journal of clinical pharmacy》2020,42(2):635-641
International Journal of Clinical Pharmacy - Background Renal anaemia worsens because of the uraemic status immediately before the initiation of haemodialysis. The haemoglobin level in patients... 相似文献
65.
66.
Yoko Uchida Fujiya Gomi Shigeo Murayama Hiroshi Takahashi 《The American journal of pathology》2013,182(5):1718-1726
67.
Mulyanto Sulaiman Ngongu Depamede Made Sriasih Masaharu Takahashi Shigeo Nagashima Suljid Jirintai Tsutomu Nishizawa Hiroaki Okamoto 《Archives of virology》2013,158(1):87-96
One hundred sixteen rats (Rattus rattus) captured in Indonesia from 2011 to 2012 were investigated for the prevalence of hepatitis E virus (HEV)-specific antibodies and HEV RNA. Using an ELISA based on HEV genotype 4 with an ad hoc cutoff value of 0.500, 18.1 % of the rats tested positive for anti-HEV IgG. By nested RT-PCR, 14.7 % of the rats had rat HEV RNA, and none were positive for HEV genotype 1-4. A high HEV prevalence among rats was associated with lower sanitary conditions in areas with a high population density. Sixteen of the 17 HEV isolates obtained from infected rats showed >93.0 % nucleotide sequence identity within the 840-nucleotide ORF1-ORF2 sequence and were most closely related to a Vietnamese strain (85.9-87.9 % identity), while the remaining isolate differed from known rat HEV strains by 18.8-23.3 % and may belong to a novel lineage of rat HEV. These results suggest a wide distribution of rat HEV with divergent genomes. 相似文献
68.
Atsushi Morita Takashi Enokizono Tatsuyuki Ohto Mai Tanaka Shiena Watanabe Yui Takada Kazuhiro Iwama Takeshi Mizuguchi Naomichi Matsumoto Masashi Morita Shigeo Takashima Nobuyuki Shimozawa Hidetoshi Takada 《Brain & development》2021,43(3):475-481
Peroxisomal acyl-CoA oxidase (ACOX1) deficiency is a rare autosomal recessive single enzyme deficiency characterized by hypotonia, seizures, failure to thrive, developmental delay, and neurological regression starting from approximately 3 years of age.Here, we report two siblings with ACOX1 deficiency born to non-consanguineous Japanese parents. They showed mild global developmental delay from infancy and began to regress at 5 years 10 months and 5 years 6 months of age respectively. They gradually manifested with cerebellar ataxia, dysarthria, pyramidal signs, and dysphasia. Brain MRI revealed T2 high-intensity areas in the cerebellar white matter, bilateral middle cerebellar peduncle, and transverse tracts of the pons, followed by progressive atrophy of these areas.Intriguingly, the ratios of C24:0, C25:0, and C26:0 to C22:0 in plasma, which usually increase in ACOX1 deficiency were within normal ranges in both patients. On the other hand, whole exome sequencing revealed novel compound heterozygous variants in ACOX1: a frameshift variant (c.160delC:p.Leu54Serfs*18) and a missense variant (c.1259 T > C:p.Phe420Ser). The plasma concentration of individual very long chain fatty acids (C24:0, C25:0, and C26:0) was elevated, and we found that peroxisomes in fibroblasts of the patients were larger in size and fewer in number as previously reported in patients with ACOX1 deficiency. Furthermore, the C24:0 β-oxidation activity was dramatically reduced.Our findings suggest that the elevation of individual plasma very long chain fatty acids concentration, genetic analysis including whole exome analysis, and biochemical studies on the patient’s fibroblasts should be considered for the correct diagnosis of ACOX1 deficiency. 相似文献
69.
Sota Iwafuchi Atsuo Kikuchi Wakaba Endo Takehiko Inui Yu Aihara Kazuhito Satou Tadashi Kaname Shigeo Kure 《Brain & development》2021,43(2):303-307
BackgroundCUL3 encodes cullin-3, a core component of a ubiquitin E3 ligase. CUL3 mutations have recently been associated with autism spectrum disorder (ASD); however, the detailed clinical courses have been described in only a limited number of patients with CUL3 mutations and neurodevelopmental diseases, including ASD.Case reportA 21-month-old Japanese girl presented with febrile status epilepticus and thereafter exhibited developmental regression, including loss of her verbal ability, eye contact, and skills in activities of daily living. Trio-based exome sequencing identified a de novo two-base insertion in CUL3, c.1758_1759insTG, p.(Thr587*).ConclusionWe report a case of a patient with ASD and a stop-gain CUL3 variant. Screening of CUL3 variants is worth considering for patients with ASD, especially those with Rett-like developmental regression. 相似文献
70.