2002年广州地区淋球菌对4种抗生素耐药性结果分析

目的了解广州地区2002年淋球菌对抗生素的耐药性及产青霉素酶淋球菌(PPNG)和高水平耐四环素淋球菌(TRNG)的流行状况。方法用琼脂稀释法测定四环素、壮观霉素、头孢三嗪和环丙沙星4种抗生素对100株淋球菌的最低抑菌浓度(MIC)及用纸片碘量法检测β-内酰胺酶。结果100株淋球菌检出PPNG 15株(15%)、TRNG 28株(28%),环丙沙星耐药率高达98%,高度耐药株(MIC≥16 mg/L)32株(32%),未发现对头孢三嗪、壮观霉素的耐药菌株,但头孢三嗪的敏感性有所下降。结论目前广州地区流行的淋球菌菌珠耐药状况仍然严重,尤以环丙沙星为甚,但对壮观霉素和头孢三嗪仍较敏感。此外,有必要持续监测球菌的耐药性。     

大鼠正畸牙移动过程中胰岛素样生长因子-Ⅰ(IGF-Ⅰ)在牙周膜中的表达

目的:观察大鼠正畸牙周膜改建过程中胰岛素样生长因子-Ⅰ(IGF-Ⅰ)在牙周膜中的表达变化,探讨IGF-Ⅰ与正畸牙齿移动的关系.方法:在大鼠上颌第一磨牙与上颌切牙之间安装正畸装置,于牙齿移动3,7,14,21, 28d后取材分别进行HE和免疫组化染色.结果:IGF-Ⅰ于牙齿移动3d后在牙周膜中表达开始增强,张力侧7d达到高峰,压力侧14d达到高峰,以后表达逐渐降低.结论:IGF-1参与了正畸牙周膜改建过程.     

Can a leukocyte depletion filter (LDF) reduce the risk of reintroduction of hepatocellular carcinoma cells?

During liver transplantation for hepatocellular carcinoma (HCC) patients, HCC could theoretically be introduced into the systemic circulation when salvaged blood is used with an autotransfusion device. Several reports have shown that some types of leukocyte depletion filters (LDFs) could completely reduce the risk for reintroducing some types of tumor cells. In this study, we tested the ability of the LDF (RCEZ1T, Pall Biomedical Co, NY, USA) to reduce the risk for reintroducing HCC cells in vitro by using a very sensitive detection method. We divided the test group into 6 groups: group I was 10 cells, group II was 20 cells, group III was 2 x 10(3) cells, group IV was 2 x 10(5) cells, group V was 2 x 10(6) cells, and group VI was 2 x 10(7) cells. The counted cells in 200 mL saline were passed through the RCEZ1T using the force of gravity. To identify the presence of cells, the pellet was resuspended, and polymerase chain reaction (PCR) was performed. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a housekeeping gene, was used as a primer. In groups I and II, the HCC cells were completely filtered in all experiments. However, in groups III, IV, and V, the HCC cells were not completely filtered in a few of the repeated experiments, with the unfiltered rate of tumor cells being between 8% and 20%. In group VI, the HCC cells were not completely filtered in all the repeated experiments. In conclusion, the RCEZ1T filter markedly reduced the risk for reintroduction of HCC cells. However, at high HCC cell load the filter cannot completely remove all the tumor cells. Further studies are required to assess the impact in clinical settings.     

SOMATOM CT高压熔断丝炸裂故障分析

简要介绍西门子SOMATOM CT高压发生器的工作原理,并着重分析了高压63A熔断丝炸裂故障原因及其判断与排除方法。文章指出了在检修高压发生器的过程中特别要注意的事项。     

Further studies on interactions between periaqueductal gray, nucleus accumbens and habenula in antinociception.

Previous findings from this laboratory with the intracerebral microinjection technique suggested that the periaqueductal gray (PAG), nucleus accumbens, and habenula might constitute a unidirectional loop to play their roles in pain modulation. In the present study we demonstrate that intra-habenular injection of naloxone antagonizes the analgesia elicited by morphine injected into the periaqueductal gray (PAG) and that intra-accumbens injection of naloxone is capable of attenuating the analgesic effects of morphine injected into the habenula. These results indicate that the relationships between these nuclei may be more complex than the putative unidirectional loop.     

卡那霉素链霉菌噬菌体SKJ1DNA限制性内切酶图谱的构建

卡那霉素链霉菌温和性噬菌体ＳＫＪ１ＤＮＡ上有２个ＡｖａＩ和ＳｍａＩ酶切位点，８个ＳａｌＩ位点，７个ＥｃｏＲＩ位点及１２个ＢａｍＨＩ位点，经酶切后用１％琼脂糖凝胶电泳测定ＳＫＪ１ＤＮＡ大小为６０．７ｋｂ。用单酶切，双酶切及片段酶切分析，得到ＳＫＪ１ＤＮＡ的ＡｖａＩ、ＳｍａＩ、ＥｃｏＲＩ、ＳａｌＩ酶切图谱及ＢａｍＩ１的部分酶切图谱，证明ＡｖａＩ和ＳｍａＩ在ＳＫＪ１ＤＮＡ上具有相同酶切位点，并经过酶切结     

钛种植体－羟基磷灰石早期修复犬下颌骨缺损与骨结合的实验研究

本研究对钛种植体－羟基磷灰石（Ｔｉ－ＨＡ）在早期修复下颌骨缺损中与骨结合的界面形态进行了扫描电镜和Ｘ线能谱观察分析。通过对９只健康狗下颌骨缺损区植入Ｔｉ－ＨＡ２、６及１２周后Ｔｉ－ＨＡ／宿主骨界面及Ｔｉ／ＨＡ界面的扫描电镜观察和Ｘ线能谱分析，结果表明Ｔｉ－ＨＡ植入骨缺损区，不引起正常的骨矿化过程改变，与宿主骨形成骨性结合；Ｔｉ／ＨＡ界面有新骨形成；扫描电镜观察和Ｘ线能谱分析在研究骨缺损修复过程中不同阶段的组织成份的变化是一个重要手段。     

经胸门奇静脉断流术的外科解剖学基础(附52例临床疗效分析报告)

观测了１００例成年尸体胃底、贲门及食管区静脉。结果显示：胃左、右静脉的出现率是９６％和９２％，胃小弯侧的静脉分布可归纳为５型：胃左静脉型、胃右静脉型、胃左静脉优势型、胃右静脉优势型和胃左右静脉均衡型。胃后静脉出现率为７３．６％，胃左静脉与左膈下静脉间有相对恒定的吻合，此种情况占４８％。在此基础上作者于１９７６年３月至１９９２年３月对５２例因门脉高压症大出血患者行经胸门奇静脉断流术，急症手术死亡率为４．９％，急症手术止血率为９２．６％，远期再出血率为１１．４％。作者认为彻底断流的关键是识别胃小弯侧静脉分型、胃左静脉汇入部位及属支变化；胃后静脉及门腔吻合。阐述了经胸门奇静脉断流的优越性。     

人巨细胞病毒感染对肾移植受者血清白细胞介素－6水平的影响

应用ＰＣＲ检测ＨＣＭＶ－ＤＮＡ，ＥＬＩＳＡ检测ＨＣＭＶ－ＩｇＭ、ＩｇＧ，诊断肾移植受者ＨＣＭＶ感染，６５例受者中ＨＣＭＶ感染者３９例，非感染者２６例。应用ＭＴＴ法检测受者血清ＩＬ－６生物活性，阐明了ＨＣＭＶ感染对肾移植受者血清ＩＬ－６水平的影响。结果表明：感染与非感染组间血清ＩＬ－６水平差异无显著性（Ｐ＞０．０５）；６例原发性感染者血清ＩＬ－６水平随感染时间延长呈增高及降低双相改变，表明慢性迁延性感染者血清ＩＬ－６水平降低。临床工作中监测ＨＣＭＶ感染的肾移植受者血清ＩＬ－６水平变化具有重要意义。     

交链孢酚单甲醚在大鼠小鼠体内的分布

对食管癌高发区粮食中分离的互隔交链孢霉的毒素交链孢酚单甲醚(AME)在大鼠、小鼠体内的分布进行研究。给动物腹腔内注射氚标记的交链孢酚单甲醚(~3H-AME),然后在不同时间测各脏器的放射性。结果表明:在所测9个脏器中,2h肝脏含量最高,食管下段在大小鼠分别居第四和第三。但大小鼠分别在72、24h则以食管下段最高,说明AME及其代谢物在此清除较慢,即对其有较高的亲和力。