Human cord blood conditioned medium enhances leukemia colony formation in vitro

Childhood T-cell acute lymphoblastic leukemia (T-ALL) is one of the most common childhood cancers. Recently, we observed that pre-conditioning sub-lethally irradiated immunodeficient mice with human cord blood mononuclear cells facilitates in these mice high level engraftment of primary T-ALL cells obtained from patients. Here we report that human cord blood cells secrete a factor(s) which markedly enhances in vitro both colony number and burst size of the T-ALL clonogenic progenitors from patients. The enhancing activity does not correspond to IL-2, IL-15, nor to several other cytokines implicated in T cell proliferation/activation. Thus, it is possible cord blood may secrete an as yet unidentified factor(s) acting on leukemia clonogenic progenitors of T-ALL. Collectively, these studies should prove invaluable in addressing the growth properties of primary T-ALL cells from patients.     

Identification of the subcellular localization of daunorubicin in multidrug-resistant K562 cell line

We examined the subcellular distribution of daunorubicin (DNR) in resistant K562 cell line which overexpress the P-glycoprotein by confocal laser scanning microscopy. Three fluorescent probes - Rhodamine123, neutral red, NBD-ceramide, which stain the mitochondria, lysosomes, Golgi apparatus respectively, were used to identify the nature of the subcellular compartment sequestering daunorubicin. In sensitive k562 cell line, nuclear and cytoplasmic DNR fluorescence was intense and diffuse. In contrast, resistant K562 cell line showed a different DNR distribution. A bright fluorescence signal was located in the perinuclear region and peripheral plasma, the nucleus and other cytoplasmic region appear as empty, as suggested by the distribution of fluorescent probe Rhodamine123 specifically for mitochondria. Verapamil, an effective resistance modulator in P-glycoprotein MDR cells, restored the DNR distribution closer to that in the parent cells. Golgic inhibitor brefeldin A and lysosomotropic agent chloroquine had little effect on drug sequestration. Our studies demonstrate that daunorubicin may be sequestered in mitochondrial compartment in the resistant cells and P-glycoprotein plays an important role on mediating DNR transport.     

健康青年男女脊柱区红外热像图的定位、定量研究

目的 :通过对健康青年男女脊柱区红外热像图的定位、定量研究 ,为医用红外热像技术的应用提供局部的相对标准参数。方法 :采用国产DW— 910 0医用红外热像仪 ,摄取正后位热像图 ,测量脊柱区不同节段的最高温度、最低温度和平均温度。结果 :(1)健康男女青年脊柱区红外热像图的平均温度分别为 (3 0 0 1±1 0 5 )℃和 (2 9 5 4± 1 19)℃。 (2 )健康男性青年脊柱区各节段红外热像图的平均温度均高于女性。结论 :健康青年男女脊柱区红外热像图的定位、定量研究 ,为正确分析人体脊柱区红外热像图提供了相对标准参数 ,具有重要的应用参考价值     

Effects of 4-MAPC,a 5α-reductase inhibitor,and cyproterone acetate on regrowth of the rat ventral prostate

Inhibitors of 5α-reductase activity cause less involution of the rat ventral prostate (VP) than does castration. Studies were conducted in adult Sprague Dawley rats to evaluate the effects of a potent 5α-reductase inhibitor, 4-MAPC, and the antiandrogen, cyproterone acetate (CA), on DNA synthesis and apoptosis. In experiment 1, VP weight fell 33%, 53%, and 83%, and DNA per ventral prostate was reduced 24%, 46%, and 71%, by 4-MAPC, CA, and castration, respectively. In experiment 2, adult rats were castrated, and the VP involuted for 7 days prior to 3 daily injections of testosterone propionate (TP; 1 mg/kg/d) ± 10 mg/kg/d of 4-MAPC or CA. ³H-thymidine incorporation into VP DNA was increased in castrated animals treated with TP, and 4-MAPC and CA reduced uptake. In experiment 3, animals were treated for 14 days with the same protocol as that used in experiment 2. VP weight was increased in all animals treated with TP when compared with castration, and was reduced by both 4-MAPC and CA. DNA in rats treated with TP was similar to that in intact animals. DNA was not reduced by 4-MAPC, but was reduced by CA. The mRNA for TRPM-2, a marker of apoptosis, was increased only in untreated castrated rats. It appears that CA has a greater inhibitory effect than 4-MAPC on DNA synthesis. A major reason why castration reduces DNA more than either 4-MAPC or CA is that neither of these agents was able to increase programmed cell death to the degree seen with castration. © 1994 Wiley-Liss, Inc.     

宁国贝母生物碱的分离和结构鉴定

从贝母新种——宁国贝母(Fritillaria ningguoensis S. C. Chen et S. F. Yin)鳞茎中分离出五个生物碱,其中碱V是一种新的生物碱,命名为宁贝新(ningpeisine),根据理化常数和光谱解析以及衍生物制备,测定其结构为N-methyl-3β-hydroxy-5α-veratranine-6-one。其余四种生物碱鉴定为浙贝甲素(peimine,verticine,Ⅰ),浙贝乙素(peiminine,verticinone,Ⅱ),异浙贝甲素(isoverticine,Ⅲ)和贝母辛(peimisine,Ⅳ)。     

改良乳房下锥形真皮瓣巨乳缩小术

为进一步保证巨乳缩小术后乳头乳晕的血供和感觉，简化手术操作。设计乳房下锥形真皮瓣，同时把皮肤磨削术应用于巨乳缩小术。自１９９４年１月至１９９７年６月为８例（１６侧）巨乳患者实施手术。全部患者术后乳头乳晕无并发症。并能大大缩短手术时间。认为皮肤磨削术乳房下锥形真皮瓣不仅可以确保乳头、乳晕的血供和感觉，且可以大大缩短手术时间，减少术后并发症     

Viral etiology of chronic cervicitis and its therapeutic response to a recombinant interferon.

Chronic cervicitis was shown to be related to papillomavirus type 16(HPV-16), herpes simplex virus type 2 and cytomegalovirus (CMV) infections as demonstrated by DNA hybridization technique and virus isolation method from samples taken from erosive and normal cervices. After one course of treatment with recombinant interferon alpha 1 (rIFN-alpha 1), 93.8% of cases showed clinical improvement and 60% marked improvement. The HPV-16 and HSV detection rates dropped down significantly after rIFN-alpha 1 treatment as compared with those before treatment. Astragalus membranaceus, a Chinese herbal drug, was shown to be synergic to interferon therapy.

     

病毒唑联合硝苯吡啶治疗病毒性肠炎疗效观察

用病毒唑和硝苯吡啶联合治疗婴幼儿病毒性肠炎１００例，同时设对照组ｌ００例给予一般治疗。结果显示治疗组平均止泻时间３．１±０．８４天，对照组平均止泻时间４．５７±１．１７天，两组差异有显著意义（χ２＝５．５９，Ｐ＜０．０１）。     

小诺霉素的药物动力学研究

报告微生物法测定血清中小诺霉素浓度,并用于家兔静脉和肌内给药及细菌性感染患者肌内给药的药物动力学研究。家兔静脉给药5mg/kg,药物动力学方程为C=~-27.36e~(-13.65(?))+22.07e~(-0.505t);家兔肌内给药5mg/kg,药物动力学方程为c=15.10(e~(-0.4(?)7)-e~(-5.143t)。吸收迅速完全,30min 可达高峰。在10例感染性疾病患者中,一次肌内给药120mg 小诺霉素,吸收迅速,平均达峰时间为0.76h,平均峰浓度9.61ug/ml,平均消除半衰期为2.63h。个体差异较大。