左旋18-甲基炔诺酮肟与左旋18-甲基炔诺酮的抗生育作用比较

本文比较了LNGO和LNG对大鼠的抗生育作用,结果表明,LNGO在剂量为10mg/kg·d时可以完全抑制大鼠着床,剂量为40,60mg/kg·d时有明显抗早孕作用。光电镜观察提示,剂量为10mg/kg·d时,对大鼠子宫内膜间质细胞和上皮细胞均有影响。组织培养研究发现,对体外人胎盘滋养层细胞有直接损伤作用。LNG对大鼠未见抗着床及抗早孕作用,对大鼠子宫内膜和体外人胎盘滋养层细胞也均未见明显作用。     

Accidental caustic ingestion in Tunisian child. Study of 330 cases

We studied retrospectively 330 cases of caustic product ingestion at the child collected in the pediatric department of Sousse (CHU Sahloul and CHU Farhat Hached) during eight years (1993-2000). It is about 194 boys and 136 girls (sex-ratio to 1.42/1) aged of 4 months at 14 years (middle age at 3 years and 5 months). Concerned products are dominated by the water of bleach (55.7%) dilute essentially (49%), caustic soda (27.9%), diluent of painting (8.5%), potash (2.7%) and the acidic products (2.2%). Endoscopy showed oeso-gastric lesions in 89% of cases: oesophagitis stage I (73.5%), stage IIa (11%), stage IIb (4%), stage III (11.2%) and an inflammatory sténose case of straightaway. The associated gastric lesions have been found in 15.1% of the cases. The recovery was the rule for all patient presenting a benign oesophagitis. The 46 cases of severe oesophogitis have been treated according two protocols: --A group (n=1 ), treated by parenteral food with treatment by antibiotics (1993-1994). --B group (n=35), treated by high dose of corticosteroids (Méthyl-prednisolone) aiming to warn esophageal sténosis (1995-2000). Three patients of the A group and five of the B group developed stenosis with statistically meaningful difference (p=0.44). Among these eight patients, six required oesophagoplasty and two had a good evolution after esophageal dilation.     

人成釉蛋白C端肽的大量表达及纯化

目的:获得高纯度的重组人成釉蛋白C端肽。方法:实验于2001-01/2004-06在解放军第四军医大学口腔内科实验室解放军第四军医大学基础部生物化学与分子生物学实验室完成。①转化pRSET-A/成釉蛋白原核表达质粒入BL21(DE3)细菌进行大量培养,加入诱导剂IPTG至终浓度为0.1mmol/L,32℃继续振荡诱导培养5h,收集细菌,诱导菌体的裂解上清经金属螯合亲和层析,洗脱Ni-NTA结合蛋白,收集洗脱蛋白,聚丙烯酰胺凝胶电泳法鉴定。②离子交换层析缓冲液(pH 7.4,50 mmol/L Tris·Cl)平衡Q SapharoseHP层析柱,将初步纯化的蛋白样品加在Q Sapharose HP凝胶介质上,梯度洗脱结合的蛋白,收集洗脱峰,制样,聚丙烯酰胺凝胶电泳法鉴定洗脱蛋白。结果:①表达重组人成釉蛋白C端肽的金属螯合亲和层析结果:诱导菌体的裂解上清经金属螯合亲和层析,洗脱下Ni-NTA结合蛋白,经聚丙烯酰胺凝胶电泳法电泳证实获得初步纯化的重组人成釉蛋白C端肽,其中仍含一些杂蛋白。②重组人成釉蛋白C端肽的离子交换层析结果:获得多个蛋白洗脱峰,聚丙烯酰胺凝胶电泳法证实获得纯化的重组人成釉蛋白C端肽。结论:获得了纯化的人成釉蛋白重组蛋白。     

中药桔丙酯联合降糖治疗对2型糖尿病患者甲襞微循环及血液流变学的影响

目的观察中药桔丙酯注射液联合降糖治疗对2型糖尿病甲襞微循环及血液流变学的影响,探讨其治疗机制.方法于2005-10/2006-08选择河北北方学院附属校医院、张家口市第一人民医院门诊及住院收治的2型糖尿病患者48例,为治疗组.应用注射用桔丙酯治疗,桔丙酯180 mg,次,1次,d,连用2周;健康志愿者30例为对照组.采用微循环显微镜,于治疗前后常规检查左手第4指甲襞第一排管袢,按田牛加权积分法对16项指标综合定量分析,判断疗效和甲襞微循环的变化.同时观察2型糖尿病患者治疗前后血液流变学各项指标的变化.结果①治疗组治疗前甲襞微循环及血液流变学各项指标均表现为不同程度的异常,治疗后甲襞微循环及血液流变学各项指标明显改善.甲襞微循环由治疗前的中、重度异常恢复到大致正常,治疗后总积分值低于治疗前(分别为1.52±0.69,4.52±0.82,t=3.96,P＜0.05).②血液流变学指标变化表明,血浆黏度、全血黏度、纤维蛋白原及红细胞电泳等各指标经桔丙酯治疗后均明显降低(P＜0 05).结论中药桔丙酯联合降糖治疗能够明显改善2型糖尿病患者血流变异常及微循环障碍.     

尿液中利尿剂、丙磺舒、咖啡因、匹莫林的固相提取和HPLC初筛分析

利尿剂广泛应用于治疗高血压和水肿。近年来,利尿剂在体育竞赛中被滥用。丙磺舒是一种抗痛风药,因该药主要在肾脏排泄,竞争性的抑制了其它药物(如甾体类药物)的排泄,为此服用过甾体类药物的运动员用它来帮助逃避药物检查。国际奥委会在1988年奥运会上正式禁用利尿剂和丙磺舒。咖啡因和匹莫林属中枢兴奋剂,因其挥发性差,气相检测困难,因此也同利尿剂一起分析。     

锕系核素促排药物——多元酚氨羧酸衍生物的合成

放射性元素的应用日益广泛。因放射性污染而造成的放射性核素进入体内并长期存在,是致癌的一个重要因素。使用螯合剂DTPA加速排出体内的放射性元素是临床上常用方法之一。但长期使用DTPA会造成体内所必需的二价金属离子的衰竭而影响人体正常代谢。同时DTPA对~(234)钍的促排效果也不理想。一类新的邻苯二酚类螯合剂LICAM系列用于促排放射性核素钚,动物实验效果较好,但未见促排~(234)钍的报道。     

含羟基氨基酸小肽的合成及其抗孕酮生成活性

用固相、逐步接肽法合成了十一个含羟基氨基酸小肽。在肽树脂裂解反应中比较了HF及TFMSA(三氟甲磺酸)两种方法,裂解收率基本相同。全部产物经C-18反相柱纯化后,氨基酸组成数值均符合预期结构要求。对孕酮分泌影响的活性筛选表明Gly-Tyr-NH₂,Gly-Tyr-Lys-OH及Gly-Ser-Lys-OH有明显的抑制hCG诱发孕酮分泌的活性(P<0.01)。     

Clinical,biochemical and molecular characterization of peroxisomal diseases in Arabs

Shaheen R, Al‐Dirbashi OY, Al‐Hassnan ZN, Al‐Owain M, Makhsheed N, Basheeri F, Seidahmed MZ, Salih MAM, Faqih E, Zaidan H, Al‐Sayed M, Rahbeeni Z, Al‐Sheddi T, Hashem M, Kurdi W, Shimozawa N, Alkuraya FS. Clinical, biochemical and molecular characterization of peroxisomal diseases in Arabs. Peroxisomes are single membrane‐bound cellular organelles that carry out critical metabolic reactions perturbation of which leads to an array of clinical phenotypes known as peroxisomal disorders (PD). In this study, the largest of its kind in the Middle East, we sought to comprehensively characterize these rare disorders at the clinical, biochemical and molecular levels. Over a 2‐year period, we have enrolled 17 patients representing 16 Arab families. Zellweger‐spectrum phenotype was observed in 12 patients and the remaining 5 had the rhizomelic chondrodysplasia punctata phenotype. We show that homozygosity mapping is a cost‐effective strategy that enabled the identification of the underlying genetic defect in 100% of the cases. The pathogenic nature of the mutations identified was confirmed by immunofluorescence and complementation assays. We confirm the genetic heterogeneity of PD in our population, expand the pool of pathogenic alleles and draw some phenotype/genotype correlations.