不同骨面型成人骨性安氏Ⅲ类牙颌畸形非手术治疗的疗效分析

目的观察不同骨面型骨性安氏Ⅲ类成人患者非手术治疗疗效的异同。方法收集北京大学口腔医学院正畸科1997—2000年就诊的非手术治疗骨性安氏Ⅲ类成人患者21例,根据不同骨面型及拔牙与否分成3组,分别对各组16项测量项目治疗前后测量值进行配对样本t检验。结果骨性Ⅲ类成人患者非手术治疗后骨骼变化有限,咬合关系的改善主要靠牙齿的代偿移动。牙齿的代偿与患者颅面形态和拔牙与否有关。结论不同骨面型或不同类骨性安氏Ⅲ类成人患者非手术治疗的疗效不尽相同。     

中药及提取物抑制肿瘤血管生成抗转移的研究进展

转移是恶性肿瘤的基本生物学特征,是临床上绝大多数肿瘤病人的致死因素^[1]。1971年Folkman^[2]就提出恶性肿瘤的一大特性是无限制地侵袭性生长和远处转移,这一特性依赖于肿瘤血管的生成,因此抑制血管生成能显著抑制肿瘤的生长和转移。目前抑制肿瘤血管生成抗转移的途径主要有：①阻断或抑制促血管生成因子通路。     

莪术醇通过降解ECM改变细胞周期抑制A549细胞增殖

目的:探讨莪术醇抑制A549细胞增殖作用及分子机理。方法:MTT法观察莪术醇对A549细胞增殖的抑制作用;天狼猩红染色法检测莪术醇对A549细胞层分泌胶原的影响;流式细胞术检测细胞周期阻滞。结果:莪术醇对A549细胞增殖有抑制作用,并能抑制细胞胶原分泌;细胞周期分析显示,莪术醇作用细胞24h,主要通过增加G1期细胞比例来抑制细胞增殖,作用48h主要影响了细胞的G2期。结论莪术醇能抑制肺腺癌A549细胞增殖,其机制可能是与莪术醇抑制ECM中胶原的分泌、诱导细胞周期阻滞有关。     

Analgesic and anticonvulsant activity of new derivatives of 2-substituted 4-hydroxybutanamides in mice

Earlier in vitro studies of the compounds marked as GT27, GT28, GT29 and BM128 revealed their inhibitory action towards murine γ-aminobutyric acid (GABA) transporters (mGAT1-mGAT4). In the present paper, the pharmacological activity of four γ-hydroxybutyric acid (GHB) amide derivatives was investigated. The following procedures were involved: locomotor activity, hot plate and electroconvulsive threshold tests. The compounds' influence on motor coordination was evaluated in the chimney test, as well. Intraperitoneal (i.p.) administration of the GHB derivatives decreased animals' locomotor activity (ED(50) values ranged between 23.79 and 26.37 mg/kg). At a dose of 25 mg/kg (i.p.) the compounds prolonged the nociceptive reaction time latency in the hot plate assay to various degree and GT28 and GT29 were the most potent ones in this respect. Their analgesic efficacy was particularly pronounced 30 min after their administration [percent of maximal possible effect (%MPE) = 16.93 and 22.72, respectively]. The investigated GHB derivatives, except for GT29 at 100 mg/kg, increased the electroconvulsive threshold by approximately 4-11 mA as compared to the vehicle-treated mice. In the chimney test they impaired the animals' motor coordination to various degree. We suggest further investigations of the compounds to estimate their biological activity.     

Retraction: In situ Raman investigation of the phase transition of NaVO2F2 under variable temperature conditions

Retraction of ‘In situ Raman investigation of the phase transition of NaVO₂F₂ under variable temperature conditions’ by Sa Zhang et al., RSC Adv., 2021, 11, 23550–23556, https://doi.org/10.1039/D1RA02827H.

We, the named authors, hereby wholly retract this RSC Advances article due to issues with the equipment used during the research and the resulting errors due to an unstable temperature control system.A Raman spectrometer was used for the testing outlined in this research, utilising coupled variable temperatures to investigate the phase transition of NaVO₂F₂. The temperature change was controlled by a THMS600 high and low-temperature sample stand. After the publication of this research, the unstable temperature was found to be caused by the system of the THMS600 sample stage, which lead to the temperature error in the analysis of phase transformation during the heating process. This has led to errors in the analysis, and therefore the conclusions of this paper are not supported. The authors also regret that the funding information was incorrectly shown in the original article.Signed: Sa Zhang, Yan Li, Liuqing Huang, Liuying Huang and Xuetao Luo, 23rd June 2022.Retraction endorsed by Laura Fisher, Executive Editor, RSC Advances.     

新疆民族药多伞阿魏挥发油成分与土壤因子相关性研究

目的 研究土壤因子对多伞阿魏挥发油成分的影响,筛选影响其含量的主导因子。方法 测定不同产地多伞阿魏挥发油成分含量及相应生长地土壤养分和pH值等,采用单因素方差分析、相关分析和偏最小二乘法和灰色关联等统计方法分析土壤因子对多伞阿魏挥发油成分含量的影响。结果 不同产地多伞阿魏挥发油成分含量和土壤因子均有显著差异。多伞阿魏12种挥发油成分总含量与土壤pH值呈显著负相关(P<0.05);愈创木醇与速效钾呈显著负相关(P<0.05);α-蒎烯与土壤速效氮、速效钾呈显著正相关(P<0.01);莰烯、右旋柠檬烯与土壤速效钾呈显著正相关(P<0.05);异愈创木醇与全磷呈显著正相关(P<0.05);β-金合欢烯与速效磷呈显著负相关(P<0.05)。偏最小二乘法分析表明,土壤pH值、速效钾含量对多伞阿魏挥发油成分的积累具有重要意义。灰色关联分析表明,土壤pH值、含水量和速效钾与多伞阿魏挥发油成分关联度较大。结论 不同产地多伞阿魏挥发油成分含量和土壤因子均有显著差异。各土壤因子对多伞阿魏挥发油成分的影响各有不同,土壤pH值、含水量和速效钾的含量可能是影响多伞阿魏挥发油成分含量的主导因子。     

维药硬尖神香草中熊果酸的薄层鉴别方法研究

目的:以熊果酸为对照品,建立新疆维吾尔药材硬尖神香草的薄层色谱鉴别方法。方法:收集两个产地8个批次的硬尖神香草生药材,根据2010版《中国药典》附录VI B(薄层色谱法)中相关项下方法进行薄层鉴别,以熊果酸作为对照品,考察不同展开系统、显色剂、温度、湿度、点样量和不同厂家硅胶板对尖神香草中熊果酸薄层鉴别的影响,从中选出维药硬尖神香草中熊果酸薄层鉴别的最佳条件。结果:吸取供试品溶液和对照品溶液各3μl点于青岛海洋化工有限公司产高效硅胶板上,在三氯甲烷-丙酮(12∶1)系统中展开,喷以10%硫酸乙醇显色,供试品与熊果酸对照品在同样的位置显紫色的斑点,所得的薄层色谱分离效果较好。结论:研究建立的硬尖神香草药材中熊果酸的薄层鉴别方法操作简便,重现性好,专属性强,可以用于维吾尔药硬尖神香草的质量控制。     

Agreement among insulin sensitivity indexes on the diagnosis of insulin resistance in polycystic ovary syndrome and ovulatory women

OBJECTIVE: The objective was to compare agreement on the diagnosis of insulin resistance (IR) among insulin sensitivity indexes in both ovulatory women and those with polycystic ovary syndrome (PCOS). STUDY DESIGN: In an observational study, the 75-g oral glucose tolerance test was performed in 105 women with PCOS and 51 ovulatory women. The insulin sensitivity indexes used were insulin quantitative sensitivity check index (QUICKI), 1/homeostasis model assessment-insulin resistance (1/HOMA-IR), area under curve for insulin (AUC-I), and the Matsuda insulin sensitivity index (COMP). For the IR diagnosis we used cut-off values described in recent publications (insulin >12 microIU/ml, 1/HOMA-IR <0.47, QUICKI < or =0.333, AUC-I > or =7000 microIU/ml 120 min, and COMP <4.75. RESULTS: Excellent agreement was assessed among insulin, QUICKI, and 1/HOMA-IR. However, the rate of IR detected by these indexes in the PCOS group (44.8-51.4%) was lower than expected. New cut-offs were then determined based on COMP results. Using these values, 1/HOMA-IR and QUICKI showed excellent agreement (kappa=0.83) with COMP. CONCLUSION: The observed agreements among insulin, QUICKI and 1/HOMA-IR were higher than 93%. Therefore, clinicians may choose any of those obtaining similar results. For clinicians who prefer COMP, but are looking for a simpler test to detect IR in PCOS women, the use of QUICKI and 1/HOMA-IR with the new cut-offs seems reasonable.     

流式细胞术检测细胞周期分析软件的比较

目的探讨流式细胞术中不同细胞周期分析软件在细胞周期分析中的特点和差异。方法采用碘化丙啶染色,在Epics XL流式细胞仪上检测细胞周期,分别应用MultiCycle和Modfit LT软件对检测数据进行分析,并比较分析结果。结果 MultiCycle和Modfit LT软件分析细胞周期各时相比例、DNA倍体分析、细胞增殖及细胞凋亡数值,比较其结果基本一致。仅人肺癌细胞系A549细胞G2/G1值应用2种不同软件分析其数据差异有统计学意义(P<0.01);Jurkat T细胞聚集体的比例应用2种不同软件分析其数据差异有统计学意义(P<0.01)。结论 MultiCycle和Modfit LT软件分析同一样本所得拟合结果有较高的一致性;对G2/G1值进行限制的操作可能更适用于组成成分较复杂的样本;虽然不同采集软件区分粘连细胞和细胞集落的方法不同,导致聚集体的比例差异显著,但并未对细胞周期分析结果的判定产生影响。     

不同超声造影参数在胰腺原发性良恶性病灶鉴别诊断中的应用

目的 探讨不同超声造影参数及模式对胰腺原发性良恶性病灶的鉴别诊断价值,以期能为诊断提供参考.方法 选择2010年6月～2013年6月赤壁市人民医院常规超声、CT等检查初步拟诊为胰腺原发性占位性病变的患者64例,其中病理证实为原发性胰腺恶性病变者48例,良性病变者16例;回顾性分析患者的临床资料,分析超声造影检查参数在良恶性病灶间的差异性;比较超声造影下胰腺原发性良恶性病灶与正常胰腺组织时间-强度曲线的不同参数指标.结果 ①超声造影低增强模式下进行胰腺良恶性病变的诊断敏感性为100.00％（48/48）、特异性为50.00％ （8/16）、阳性预测值为85.71％（48/56）、阴性预测值为100.00％（8/8）;超声造影晚增强模式下进行胰腺良恶性病变的诊断敏感性为75.00％（36/48）、特异性为12.50％（2/16）、阳性预测值为94.74％（36/38）、阴性预测值为53.84％（14/26）.②时间-强度曲线参数比较：恶性病变相对上升时间、相对平均渡越时间、相对最高增强百分数为（-1.27±4.03）s、（6.37±18.37）s、（3.84±23.24）％,良性病变为（0.97±3.12）s、（-7.50±20.18）s、（-32.37±42.15）％,两者比较差异有统计学意义（t=2.03、2.55、4.33,P＜0.05）;相对达峰时间、相对到达时间恶性病变为（-1.10±4.80）s、（-0.61±5.31）s,良性病变为（1.73±6.86）s、（1.02±6.30）s,两者比较差异无统计学意义（t=1.83、1.01,P＞0.05）.结论 超声造影检查能通过观察时间-强度曲线参数对胰腺原发性占位病灶进行鉴别诊断,具有较高的临床应用价值.