11β-羟类固醇脱氢酶与2型糖尿病

11β-羟类固醇脱氢酶(11β-HSDs)催化氢化可的松(皮质酮)和可的松(脱氢皮质酮)的相互转化，对调节局部糖皮质激素的活性起着重要作用，而糖皮质激素可诱导胰岛素抵抗。肝、脂肪等组织内11β-HSD活性的改变与肥胖、2型糖尿病等疾病密切相关。     

冠状动脉介入诊断与治疗所致低血压的观察与护理

随着21世纪的到来,中国已经跨入老龄社会,心血管病已成为威胁人类生命的头号杀手.介入诊断和治疗技术的发展有效地降低了心血管病的病死率,但是在术中或术后出现的低血压却是极为危险的,可威胁病人生命,故及时发现及时正确处理此急症是非常重要的.本研究就此问题发生的原因及护理进行分析.     

脑梗死后再发缺血性卒中与脑出血患者微血管病变及预后的比较

【目的】探讨脑梗死后再发缺血性卒中与脑出血患者的临床特点、影像学上微血管病变及预后的异同。【方法】连续收集急性复发性卒中的住院病例（初发事件为脑梗死，复发事件包括脑梗死或脑出血），详细记录临床资料。观察脑内微出血及自质病变的严重程度，随访有无严重血管事件及死亡的发生。【结果】175例再发脑梗死患者。19例复发事件为脑出血的患者进入本研究。155例（88．6％）再发脑梗死患者，以及18例（94．7％）脑出血患者存在自质病变。77例（44％）再发脑梗死患者和16例（84．2％）脑出血患者存在脑内微出血。脑出血组自质病变的严重程度及微出血的数目均明显高于再发脑梗死组（P=0．033，0．016），脑出血组的死亡率及再发脑出血的危险性明显增高（P=0．034，0．036）。【结论】严重的脑自质病变及微出血的出现与脑梗死后再发脑出血有关。多发微出血提示出血风险增高。如复发事件为脑出血，则其死亡率及再次复发脑出血的风险均明显增加。     

K562／ADM耐药细胞株的建立及其生物学特性的初步观察

我们建立的K562/ADM耐药细胞株,在ADM浓度为2.4μg/m1(4.46μM)中已稳定培养3.5个月,传了30—35代,K562/ADM亦具有多药耐受件(Multidrug Resistance,MDR)的特点,对ADM、VCR、AT—1258和DDP的耐受性分别为K562的114.7、94.0、13.3和7.4倍,但对5—FU不产生交叉耐药。K562和K562/ADM的倍增时间分别为19.2h和52.8h,集落生成率分别为37.5％和11.1％,K562染色体数为34—68,中位数为56;K562/MDM染色体数为32—90,中位数为50,K562/ADM可做为耐药机理和克服耐药措施研究的极好模型。     

Studies on monoclonal anti-isotypic and anti-idiotypic antibodies against leukemia and myeloma: IV modulation of membrane fluidity of leukemic cell lines and tonsillar cell stimulated with McAbs

Summary In this study the technique of labelling the cell membrane with DPH fluorescence polarization was used to observe the membrane fluidity of B lymphocytic cell lines and tonsillar cells from healthy persons; the modulation effect on membrane-fluidity induced by McAbs against isotypic and idiotypic determinants of IgM from patients with leukemia was studied as well. The expression of the corresponding isotypic and idiotypic determinants of IgM on the cell membrane was determined. The results show that the membrane fluidity of leukemic cell lines is remarkably higher than that of tonsillar cells from healthy persons, and McAbs against isotypic determinants of leukemic IgM can enhance the membrane fluidity of all kinds of cells mentioned above. However, the anti-idiotypic monoclonal antibody increased only the membrane fluidity of leukemic cell lines. These results indicated that there was a close relationship between the effect of McAbs on cell membrane fluidity and the expression of corresponding isotypic and idiotypic determinants of IgM on the cell membrane.     

浅论记者的职业心理动机

采访与写作是新闻记者的一种社会性活动，社会的政治经济动向、科教文化潮流、外交军事突变以及各种风云人物的活动都是这一工作反映的内容。要写出一篇好的作品．必须要有一定的社交能力、应变能力、观察能力及写作能力．这是脑力劳动和体力劳动的综合行为。当记者在枯燥的材料堆里翻开资料，当他与一个初次采访的人物去谈话．在面临危险的环境中找新闻时……．是何种动机支配着记者去克服困难．完成这些采访任务的呢？心理学认为动机是直接推动个体活动以达到一定目的的内部动力。     

Physical and spectroscopic methods for the evaluation of the interactions of antimitotic agents with tubulin.

The physico-chemical methods for the study of the binding of ligands to tubulin are examined in-depth, emphasizing the assumptions on which they are based and their limitations. The criteria of specificity and linkage to protein self-association are presented. It is shown that, of the direct equilibrium binding techniques, Hummel-Dreyer gel permeation chromatography and rapid ultracentrifugation are applicable only when binding is not linked to protein self-association. Disc filtration is valid only when the reverse unbinding reaction is very low. Binding linked to protein self-association can be measured by batch gel permeation or by dialysis equilibrium. The indirect techniques, such as fluorescence perturbation or difference absorption spectroscopy are discussed in terms of the assumptions on which they are based. They are shown to be used best only after characterization of the binding by direct techniques. Equilibrium binding parameters can also be deduced from careful kinetic experiments. Comparison of calorimetrically measured enthalpies of binding to van't Hoff enthalpies derived from equilibrium measurements indicates that the method of choice is calorimetric, while comparison with van't Hoff analysis can reveal the existence of reaction steps not detected by equilibrium measurements. Use of other indirect approaches, such as titration of an enzymic activity, can also lead to the detection of additional steps. The criteria are set up for the proper data analysis of ligand binding linked to protein self-association and the selection of the proper mode of linkage. It is shown how the thermodynamic contributions of various moieties of a ligand can be established by a rational structural modification of the ligand and the proper analysis of the binding measurements, in which all non-specific entropic contributions are taken into account. It is demonstrated also how a similar analysis of binding data can lead to conclusions about the reaction pathway from a comparison of equilibrium thermodynamic measurements on judiciously modified ligand molecules.     

青光眼小梁切除术后浅前房临床分析

目的 探讨青光眼小梁切除术后浅前房的原因及发生情况。方法 回顾总结我院1997年1月至2001年12月连续92例104只眼小梁切除术后发生浅前房的程度及原因。结果 本组发生术后浅前房16眼；占15.4％，其中结膜漏1眼(6．25％)，滤过过强11眼(68．75％)，脉络膜睫状体脱离4眼(25％)。结论 青光眼小梁切除术后浅前房原因复杂，与术前眼压关系密切，与患者年龄及青光眼类型无关，最常见的原因是房水滤过过强。     

支气管扩张症1例

患儿,男,12岁,以反复咳嗽、咯痰5年、加重2个月为主诉入院。5年前开始反复咳嗽,咯黄痰,每年至少发作4次,每次持续10~20 d。近2个月病情明显加重,无咯血,盗汗较重,体质量无减轻,无结核接触史。查体:神清,呼吸平稳,营养中等,咽充血,扁桃体Ⅰ度大。双肺底可闻及密集吸气末中小湿音,以左肺底为主。血白细胞17.4×109/L,中性粒细胞0.84,红细胞5.0×1012/L,血红蛋白151 g/L,血沉53 mm/1 h,降钙素原1.56 ng/L,C-反应蛋白67.4 mg/L。病原学检查:肺炎支原体、肺炎衣原体、腺病毒、合胞病毒、流感病毒、副流感病毒、柯萨奇病毒、埃可病毒、腮腺炎病…