清热降浊、益气活血法治疗慢性肾小球肾炎的临床疗效

目的：探究清热降浊、益气活血中药方剂联合阿托伐他汀钙片治疗慢性肾小球肾炎（CGN）患者的临床疗效。方法：选取2017年5月至2018年5月六安市中医院收治的CGN患者90例作为研究对象,按照随机数字表法分为对照组和观察组,每组45例。对照组患者给予饮食指导,并口服阿托伐他汀钙片治疗。观察组在对照组治疗的基础上给予清热降浊、益气活血中药治疗。2组CGN患者均治疗50 d,观察并记录2组患者的疗效、血脂指标和肾功能指标。结果：与治疗前比较,2组CGN患者的总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白（LDL）、血清尿素氮（BUN）、血肌酐（Scr）和24 h尿蛋白定量均明显降低,差异有统计学意义（均P<0.05）,且观察组患者的TC、Scr、BUN和24 h尿蛋白定量水平比对照组降低更显著（均P<0.05）。观察组患者的总有效率为93.33%,对照组患者的总有效率为84.44%,差异有统计学意义（P<0.05）。结论：清热降浊、益气活血中药方剂联合阿托伐他汀钙片可以显著降低CGN患者的血清血脂水平,使之恢复正常,改善肾功能,具有较高的临床应用价值。     

人血管内皮生长因子shRNA真核表达载体对肝癌细胞系SMMC-7721的增殖影响

目的:使用VEGF shRNA真核表达载体转染肝癌细胞系SMMC-7721,观察对其增殖影响。方法:使用VEGF shRNA真核表达载体转染肝癌细胞系SMMC-7721,ELISA法检测转染前后肝癌细胞系SMMC-7721的VEGF蛋白表达量,检测转染前后肝癌细胞系SMMC-7721的增殖及细胞的克隆形成能力的变化。结果:VEGF shRNA真核表达载体大部分阻断肝癌细胞系SMMC-7721的VEGF蛋白表达,转染后细胞周期中,G1期细胞增多,S期细胞减少,细胞增殖能力降低;MTT法观察细胞生长速度变慢,单个细胞的克隆形成能力明显下降。结论:VEGF shRNA真核表达载体可明显抑制SMMC-7721细胞增殖能力,为将VEGF shRNA真核表达载体应用于肝癌的基因治疗提供依据。     

胆汁分离病原菌分布及耐药特征分析

目的 研究南京医科大学第一附属医院临床送检胆汁培养的主要病原菌分布及耐药菌感染特征,为临床胆道感染提供诊疗依据.方法 回顾性分析胆汁培养病原菌1264株及耐药菌感染患者的临床资料,采用VITEK 2 Compact全自动微生物鉴定及药敏系统进行细菌鉴定、药物敏感性试验,应用WHONET 5.6进行药敏结果分析,SPSS...     

基于盆底肌加强的阴道紧缩术

目的 探讨通过加强盆底相关肌群在提升阴道紧缩术临床效果中的作用及其关键操作.方法:从作者手术项目组2018年1月至2019年12月期间实施的盆底肌加强型阴道紧缩术案例中,随机选择50例作为观察对象.均采用阴道粘膜和会阴皮肤菱形切口,行粘膜下潜行分离,依次暴露会阴浅横肌、球海绵体肌和双侧肛提肌;首先拉拢缝合固定肛提肌;然...     

ST 段抬高心肌梗死急诊冠脉介入中室颤发生的分析

目的:分析ST 段抬高心肌梗死在急诊冠脉介入中发生室颤(VF)的临床特征和冠脉造影特点.方法:对50例接受急诊冠脉介入治疗的ST段抬高心肌梗死患者进行回顾性研究,根据术中有无室颤将患者分为两组,其中9例发生心室颤动(VF组),41例患者没有发生心室颤动(无VF组).比较两组的临床特征与冠脉造影的差异.结果:两组患者的基线特征相似.VF组冠脉内溶栓占33.3%,无VF组冠脉内溶栓2.4%,两组统计学上有显著性差异(P<0.05).VF组三支病变占77.7%,无VF组占33.3%,两组统计学上有显著性差异(P<0.05).结论:(1)在ST段抬高的心梗患者在急诊冠脉介入时室颤发生可能与冠脉内急性闭塞处血栓负荷有关.(2)在ST段抬高的心梗患者在急诊冠脉介入时室颤发生与冠脉病变严重程度有关.     

白头翁总皂苷碱水解产物通过线粒体通路促进肝癌细胞凋亡研究

目的研究白头翁总皂苷碱水解产物(PAHS)抗肝癌作用及其相关机制。方法采用MTT法检测PAHS对于人肝癌细胞SMMC-7721增殖的影响,并用Giemsa染色法观察细胞形态;采用Hoechst 33258染色法以及流式细胞术检测PAHS对细胞凋亡、细胞周期及线粒体膜电位的影响;采用Western blotting检测凋亡相关蛋白Cytochrome C、Caspase-3、cleaved Caspase-3和Bcl-2的表达。采用ICR小鼠腋下sc肝癌细胞H22建立体内肝癌模型,测定肿瘤生长抑制率,并通过HE染色和透射电镜观察组织形态。结果体外结果显示,PAHS能够以剂量和时间依赖的方式抑制SMMC-7721细胞的增殖,能够将肿瘤细胞的生长周期阻滞在S期,降低线粒体膜电位。PAHS可以上调Cytochrome C、cleaved Caspase-3的表达和下调Bcl-2、Caspase-3的表达。体内实验结果显示,PAHS(50、100、200 mg/kg)可以抑制小鼠肝癌细胞的生长,具有剂量依赖性。组织学观察显示PAHS组的小鼠肿瘤组织均有大面积坏死及凋亡细胞的出现。结论 PAHS可通过诱导细胞凋亡发挥抗肝癌作用,其作用机制与线粒体凋亡途径有关。     

Didymin attenuates doxorubicin-induced cardiotoxicity by inhibiting oxidative stress

Objective: This study was designed to investigate the protective effects of didymin (Did) on doxorubicin (DOX)-induced cardiotoxicity. Methods: After pretreatment with Did (2, 4, 8 mg/kg intraperitoneal i.p.) for 7 d, the male C57 mice were injected with single dose of DOX (20 mg/kg i.p.). The cardioprotective effect of Did was observed on the 7th day after DOX treatment. Results: DOX delayed body growth and caused cardiac tissue injury, oxidative stress, and mitochondrial dysfunction. Similar experiments in H9C2 cardiomyocytes showed that DOX reduced cell viability, increased generation of reactive oxygen species (ROS) and fragmentation of DNA, decreased mitochondrial membrane potential, and induced cardiomyocyte apoptosis. However, all of these adverse effects were suppressed by Did pretreatment. Did increased protein expression of glutamate-L-cysteine ligase catalytic subunit (GCL), heme oxygenase 1 (HO-1), and nuclear factor erythroid 2-related factor 2 (Nrf2). Besides, Did also induced activation of PI3K/AKT. Conclusion: These findings indicated Did prevented DOX-induced cardiac injury and apoptosis via activating PI3K/AKT/Nrf2 signaling pathway.     

不同品种威灵仙药材的质量调查

对不同品种威灵仙药材进行质量调查.优化《中国药典》2010年版威灵仙含量测定项下方法,并测定威灵仙样品中常春藤皂苷元和齐墩果酸的含量.所测30批样品中仅有3批威灵仙Clematis chinensis药材中常春藤皂苷元和齐墩果酸的含量符合《中国药典》2010年版规定的标准;东北铁线莲C.manshurica药材中齐墩果酸质量分数大于0.30％,但常春藤皂苷元含量极微;棉团铁线莲C.hexapetala中未检测到常春藤皂苷元和齐墩果酸.     

贞芪酪蛋白肽复合物血清对人肺腺癌A549细胞分化抑制作用及相关机制研究

目的 :探索贞芪酪蛋白肽(ligustri-astragali casein co-peptid, LACP)复合物对人肺腺癌A549细胞分化的诱导作用及作用机制。 方法 :MTT法检测贞芪酪蛋白肽含药血清5%,10%,15%,20%浓度对人肺腺癌A549细胞增殖的影响;通过贞芪酪蛋白肽药物血清对A549细胞核质比和端粒酶活性的影响观察其诱导分化作用;免疫细胞化学方法观察贞芪酪蛋白肽药物血清对A549细胞Bax/bcl-2基因表达的影响。 结果 :4种不同浓度的贞芪酪蛋白肽含药血清均对A549细胞增殖有抑制作用,其中以浓度为20%的贞芪酪蛋白肽血清的抑制率最高;能降低细胞的核质比,并抑制人肺癌细胞端粒酶活性的作用;贞芪酪蛋白肽血清能促进A549细胞凋亡基因bax/bcl-2的表达,与无药血清对照组比较,差异有统计学意义(P<0.01)。 结论 :贞芪酪蛋白肽含药血清对人肺腺癌细胞A549有一定抑制作用;有诱导人肺癌细胞A549分化的作用,其主要机理可能是抑制了A549细胞端粒酶活性;贞芪酪蛋白肽诱导A549细胞凋亡的作用机制之一可能是促进促凋亡基因bax/bcl-2的表达。     

十全大补汤对荷膀胱癌小鼠大剂量化疗的减毒增效作用

Objective To investigate the toxicity attenuation and efficacy potentiation effects of Shiquan Dabu Tang (SDT) on high dose chemotherapy in T739 mice with bladder carcinoma. Methods Mouse bladder carcinoma tissue was inoculated subcutaneously into T739 mice to establish tumor-beating mice model. The tumor-bearing mice were randomly divided into a CTX group (100, 200, 400 mg/Kg respectively), a SDT group (high or low dose respectively), a high-dose SDT combined with 200 mg/Kg CTX group and a control group. The body weight, diameter of tumor nodules and complete blood count were observed subsequently. Results Different doses of SDT could effectively inhibit tumor growth in mice. SDT + CTX treatment significantly prolonged the survival time of mice by 49.4±23.3 days (P<0.01, 0.05, 0.01), compared with high dose SDT treatment (17.4±5.77) days, 200 mg/kg CTX treatment (23±14.02) days and control group (11.75±2.06) days respectively. The peripheral platelet count increased more significantly in mice treated with SDT within a week as compared to mice without SDT treatment (P<0.05). The peripheral RBC count and liB concentration increased more significantly in mice treated with SDT for 2 weeks as compared to mice without SDT treatment (P<0.05). Conclusions SDT could enhance the anti-tumor effects of high dose CTX on tumor-bearing mice and reduce toxicity in its peripheral red blood cells. The results showed that SDT combined with high dose of CTX chemotherapy had toxicity attenuation and efficacy potentiation effects in tumor-beating T739 mice.