Aberrant alteration of peripheral B lymphocyte subsets in hepatocellular carcinoma patients

Although B lymphocytes are widely known to participate in the immune response, the conclusive roles of B lymphocyte subsets in the antitumor immune response have not yet been determined. Single-cell data from GEO datasets were first analyzed, and then a B cell flow cytometry panel was used to analyze the peripheral blood of 89 HCC patients and 33 healthy controls recruited to participate in our research. Patients with HCC had a higher frequency of B10 cells and a lower percentage of MZB cells than healthy controls. And the changes in B cell subsets might occur at an early stage. Moreover, the frequency of B10 cells decreased after surgery. Positively correlated with B10 cells, the elevated IL-10 level in HCC serum may be a new biomarker in HCC identification. For the first time, our results suggest that altered B cell subsets are associated with the development and prognosis of HCC. Increased B10 cell percentage and IL-10 in HCC patients suggest they might augment the development of liver tumors. Hence, B cell subsets and related cytokines may have predictive value in HCC patients and could be potential targets for immunotherapy in HCC.     

The direct effect of lipopolysaccharide on an isolated heart is different from the effect on cardiac myocytes in vitro

IntroductionLipopolysaccharide (LPS) is widely used to induce experimental animals. However, its effects on cardiac contraction is controversial. Although LPS probably induces its influence in vivo both directly and indirectly, we focused on the direct effects of LPS in this report.Material and methodsIsolated ventricular myocytes mounted on a Langendorff apparatus were perfused with LPS. The changes in cultured H9c2 cells incubated with LPS over a 3-h exposure were compared with the changes after a 24-h incubation. Apoptosis was identified using flow cytometry and Western blotting. The mRNA levels were also determined.ResultsLPS directly stimulated cardiac contractility at low doses, although it produced inhibition at higher doses. The TLR4-coupled JAK2/STAT3 pathway was identified in H9c2 cells after LPS treatment, with an increase in intracellular calcium levels. LPS dose-dependently activated hypertrophic signals in H9c2 cells and induced apoptosis at the high dose. However, apoptosis was observed in H9c2 cells after a 24-h exposure to LPS, even at low doses. This observation appears to be associated with the level of paracrine cytokines. Changes in H9c2 cells by LPS were diminished by NPS2390, an inhibitor of the calcium-sensing receptor (CaSR). LPS also promoted CaSR mRNA expression in H9c2 cells, which may be unrelated to the changes in cytokine expression influenced by an inflammasome inhibitor.ConclusionsIn contrast to the isolated hearts, LPS activated hypertrophic signals prior to apoptotic signals in cardiac cells. Thus, LPS injury appears to be associated with CaSR, which was not markedly influenced by an inflammasome inhibitor.     

影响胰腺癌早期诊断的临床因素319例分析

[目的]探讨胰腺癌早期诊断的影响因素。[方法]回顾总结319例胰腺癌病人的临赓堑征，尤基梦首发症状和首沃就诊症状。[结果]首发症状以腹痛、上腹不适、厌食、黄疸多见，出现病，例多分别为．157型、76例、67例、46例。首次就诊时，多数病人症状增多、加重，尤其是出现厌食、黄疸、腰背疼痛、乏力、消瘦等症状的病例明显增加。73例病人首次就诊没有进行必要的辅助检查，102例病人有误诊史。[结论]对于出婴腹痛、上腹不适、厌食、黄疸等症状的病例，要考虑到胰腺癌的可能，及时进行有效的辅助检查，是发现早期胰腺癌、改善病人预后的重要措施。     

紫杉醇联合5-氟脲嘧啶持续输注治疗晚期胃癌的临床研究

目的 观察紫杉醇（PTX）联合5-氟脲嘧啶（5-Fu)持续静脉输注治疗晚期胃癌的疗效和毒性。方法 对30例晚期胃癌患者第1，第8，第15天使用PTX60mg/m^2，静脉滴注；第1-15天使用5-Fu0.25g/m^2,24h中心静脉持续输注。28天为1个周期，2个周期后评定疗效。平均3.43个周期。结果 28例可评价疗效。28例中完全缓解3例。部分缓解14例，总有效率为60.7%，中位缓解期为6.4个月。中位生存期为11.5个月。毒副作用主要为剂量限制性毒性，表现为骨髓抑制。结论 紫杉醇加5-氟脲嘧啶持续静脉输注是治疗晚期胃癌较好的化疗方案。     

急性白血病患者骨髓中GST-pi的表达与活性

目的:检测GST-pi在急性白血病(acuteleukemia,AL)患者骨髓中的表达及活性,分析其临床意义。方法:采用免疫组化方法检测160例急性白血病患者骨髓GST-pi的表达,同时采用Habig的实验方法测定其中25例标本的GST-pi活性。结果:GST-pi在复发/难治组中的表达显著高于初治/敏感组,χ2=21·910,P=0·000;AML组与ALL组GST-pi表达差异无统计学意义,P>0·05;GST-pi表达阳性组的完全缓解率明显低于GST-pi表达阴性组,χ2=46·166,P=0·000。白血病组GST-pi活性明显高于正常对照组,t=7·512,P=0·002;GST-pi表达与活性高度正相关,r=0·71,P=0·004,其中有2例患者GST-pi高表达低活性。结论:GST-pi的表达及活性与AL的发生发展密切相关,GST-pi可作为判断AL预后的重要指标。     

脑卒中流行病学调查相关文献复习

  目的  了解山东省滨州市成年居民脑卒中患病现状及其危险因素,为脑卒中的预防控制提供参考依据。  方法  于2019年4 — 9月采用多阶段分层整群随机抽样方法在滨州市抽取47个监测点共6 777名 ≥ 18周岁成年常住居民进行问卷调查、体格检查和实验室检测。  结果  滨州市6 777名成年居民中,患脑卒中者361例,加权调整后患病率为3 630.0/10万,标化患病率为2 780.1/10万;多因素非条件logistic回归分析结果显示,年龄 ≥ 60岁、身体活动不足、睡眠时间过长及有脑卒中家族史、高血压和房颤是滨州市成年居民脑卒中患病的危险因素。  结论  滨州市成年居民脑卒中患病率较高,年龄较大、身体活动不足、睡眠时间过长及有脑卒中家族史、高血压和房颤的居民是脑卒中患病的高危人群。     

β-磷酸三钙骨免疫学特性的研究进展

β-磷酸三钙(β-TCP)作为植骨材料具有优良的生物相容性和骨传导性,免疫反应在决定β-TCP体内转归中起着重要的作用,β-TCP作为异物被免疫系统识别,触发免疫细胞的不同行为模式,从而影响着新骨形成.了解β-TCP植入后诱导的免疫环境和成骨之间的影响,有助于诱导最佳的局部免疫环境,为最终骨整合提供成骨和破骨的最佳平衡...     

消除健康贫困应成为农村卫生改革与发展的优先战略

疾病与贫困结局、负担之间的负向叠加与循环已成为触发中国现有贫困发生的核心驱动因素,并构成健康贫困的本质特征因素。与经济、文化贫困相比,健康贫困已成为脱贫攻坚的关键掣肘,是中国后减贫时代成果维系的重要风险和挑战。本文瞄准健康贫困概念、理论、形成机制的研究空白,创新性将健康贫困嵌入在疾病、经济、文化与教育、制度与环境的多维耦合视域下,基于文献计量与专家定性访谈锁定健康贫困的特征要素,界定并阐释多维健康贫困的概念内涵;探索性构建了基于健康能力 – 权利 – 风险三维视角的健康贫困三角理论模型,深度解构健康能力、健康权利与健康风险在健康贫困产生、扩散与固化、消除全链条过程中的作用机制与解释框架。     

淡味药内涵析议及在儿科疾病中的运用

对中药"淡"味的概念、功效内涵进行阐述,归纳和分析淡味药的四气分布、归经分布、升降浮沉特点.并总结淡味中药在儿科疾病的配伍运用,味淡之品与小儿生理特点及病理变化相应,可准确配伍淡味药以流通津液、渗灌气血、甘淡滋脾,在肾病综合征、反复呼吸道感染、泄泻、便秘等儿科常见病治疗中发挥重要作用.以期更深入理解淡味药性理论、灵活组...     

新形势下八年制医学教育的再思考

新形势下,健康中国建设和我国卫生事业发展迫切需要具有国际竞争力的医学科学家和领军人才。在现行医学教育体系中,培养具有国际竞争力的未来医学科学家和领军人才是八年制医学教育的必然选择。创新八年制医学人才培养模式应汲取长学制试办经验,直面现存问题,聚焦创新能力和创新精神,构建以研究为基础的八年制医学人才培养模式,确立“目标引领、厚植基础、面向临床、聚集创新”的办学原则,为我国卫生事业培养具有国际竞争力的未来领军人才。