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41.
Mesangial cell proliferation in long-term streptozotocin-induced diabetes mellitus in the rat and the renoprotective activity of heparin. 总被引:3,自引:0,他引:3
G Gambaro A D'Angelo D Del Prete M Ceol S Garbisa G Bertaglia M Forino B Baggio F Anglani 《American journal of nephrology》1999,19(4):530-534
At present, it is not clear whether mesangial proliferation underlies mesangial expansion in diabetic nephropathy. To address this issue and the relationship between heparin's renoprotective and antimitogenic activities, we studied three streptozotocin-induced diabetic rat groups 5 and 12 months after diabetes induction: two groups were administered a modified heparin, each with a different protocol, and two healthy rat groups, one of which was treated with the same heparin, served as controls. Untreated diabetic animals developed clear evidence of nephropathy, namely expansion of the glomerular extracellular matrix, as expressed by glomerular basement membrane thickening, and increased mesangial deposition of type IV collagen. These alterations were prevented/cured by heparin treatment. Kidney sections were processed immunohistochemically for proliferating cell nuclear antigen and smooth muscle alpha-actin which is expressed only by proliferating mesangial cells. The number of proliferating cell nuclear antigen positive nuclei and alpha-actin-positive cells per glomerulus did not differ between groups at both 5 and 12 months. In conclusion, there is no evidence that mesangial proliferation is increased in late experimental diabetic nephropathy, and heparin seems to be renoprotective through mechanisms other than antiproliferation. 相似文献
42.
A. Tubaro G. Del FaveroD. Beltramo M. ArdizzoneM. Forino M. De BortoliM. Pelin M. PoliG. Bignami P. CiminielloS. Sosa 《Toxicon》2011,57(5):755-763
The acute oral toxicity of a new palytoxin congener, 42-hydroxy-palytoxin (42-OH-PLTX), was investigated in female CD-1 mice. The toxin (300-1697 μg/kg), administered by gavage, induced scratching, jumping, respiratory distress, cyanosis, paralysis and death of mice, with an LD50 of 651 μg/kg (95% confidence limits: 384-1018 μg/kg) within 24 h. Hematoclinical analyses showed increased plasma levels of lactate dehydrogenase and aspartate-aminotransferase at doses of 600 μg/kg and above, as well as of alanine-aminotransferase, creatine phosphokinase and potassium ions at ≥848 μg/kg. Histology revealed inflammatory lesions in the non-glandular area of the stomach of mice that survived up to 24 h after gavage (424-1200 μg/kg). Although no histological alterations were seen in skeletal and cardiac muscles, changes in some plasma biomarkers (creatine phosphokinase, lactate dehydrogenase) suggested involvement of these tissues in 42-OH-PLTX oral toxicity, in agreement with epidemiological data on seafood poisonings ascribed to palytoxins. Complete recovery of the tissue and hematological changes was observed two weeks post-exposure.Furthermore, 42-OH-PLTX induced in vitro delayed erythrocyte hemolysis at concentrations similar to those of PLTX (EC50 = 7.6 and 13.2 × 10−12 M, respectively). This hemolysis could be completely neutralized by a monoclonal anti-PLTX antibody. The in vivo data, together with the in vitro data recorded for 42-OH-PLTX, seem to indicate Na+/K+-ATPase as one of the key cellular targets of this toxin. 相似文献
43.
44.
Romualdo Benigni Chiara Laura Battistelli Cecilia Bossa Alessandro Giuliani Olga Tcheremenskaia 《journal of environmental science and health part c-environmental carcinogenesis & ecotoxicology reviews》2013,31(4):422-443
This article studies alternative toxicological approaches, with new (skin sensitization, ToxCast) and previous (carcinogenicity) analyses. Quantitative modeling of rate-limiting steps in skin sensitization and carcinogenicity predicts the majority of toxicants. Similarly, successful (Quantitative) Structure-Activity Relationships models exploit the quantification of only one, or few rate-limiting steps. High-throughput assays within ToxCast point to promising associations with endocrine disruption, whereas markers for pathways intermediate events have limited correlation with most endpoints. Since the pathways may be very different (often not simple linear chains of events), quantitative analysis is necessary to identify the type of mechanism and build the appropriate model. 相似文献
45.
AbstractIn this report, we present a case of unexplained total triploidy of donor eggs fertilized by ICSI from four different male partners of different couples. Woman who served as a donor was 27?year old, had her own healthy child, and previously twice served as a donor with normal fertilizations and healthy baby born. 相似文献
46.
Lucisano Marília Pacífico da Silva Raquel Assed Bezerra de Sousa Pereira Ana Patrícia Romualdo Priscilla Coutinho Feres Magda de Queiroz Alexandra Mussolino Nelson-Filho Paulo da Silva Lea Assed Bezerra 《Clinical oral investigations》2021,25(6):3651-3662
Clinical Oral Investigations - To answer the questions: (1) Does reducing estrogen levels influence the microbial composition of the oral cavity? (2) Does the presence of periapical lesion (PL)... 相似文献
47.
Gundela Meyer Delia Galindo-Mireles Toms Gonzalez-Hernandez Agustin Castaeyara-Perdomo Romualdo Ferres-Torres 《Brain research》1986,398(1):207-211
After injections of horseradish peroxidase into the anterior cingulate cortex of the mouse and the rat, labeled neurons were observed in different nuclei of the reticular formation of the rostral medulla oblongata: ventral part of the nucleus gigantocellularis, nucleus paragigantocellularis and nucleus lateralis reticularis. Other labeled neurons lay scattered outside the nuclear boundaries, often close to the ventral-most border of the medulla. The projection to the cingulate cortex is sparse, with rarely more than 3 cells per case, but constant. Injections into other cortical areas (visual, motor and somatosensory cortex) did not produce labeling in the medullary reticular formation. 相似文献
48.
Von Willebrand factor abnormalities in IgA nephropathy 总被引:1,自引:0,他引:1
Bertaglia G; Ossi E; Casonato A; Miglietta A; Forino M; Baggio B; Gambaro G 《Nephrology, dialysis, transplantation》1997,12(3):474-479
BACKGROUND: Plasma concentration of von Willebrand factor (vWF) has been
used as an index of endothelial dysfunction. Increased release of vWF from
endothelial cells has been reported in several conditions, and there is
also evidence that dysfunctioning endothelial cells synthesize defective
molecules. In fact, unusually large vWF multimers have been described and
related to the pathogenesis of some microangiopathic diseases. Abnormal
levels of vWF have been reported in primary glomerulitis, but this was no
referred to histological diagnosis. Furthermore, no qualitative vWF
analysis was performed in these glomerulopathies. Therefore the aim of our
study was to analyse quantitatively and qualitatively vWF in patients with
IgA (IgAN) and non-IgA mesangial proliferative glomerulonephritis (PGN).
METHODS: Fourteen IgAN patients, eight PGN patients, seven subjects with
different glomerulonephritides, and 10 healthy controls formed the basis of
this study. On peripheral venous blood collected in the presence of
protease inhibitors, vWF parameters were investigated. vWF antigenic
activity (vWF:Ag) was measured by electroimmunodiffusion. vWF subunits
mobility was studied by crossed immunoelectrophoresis (CIE) and in some
patients vWF multimeric analysis was performed by SDS- agarose gel
electrophoresis. RESULTS: Mean vWF:Ag was significantly higher in PGN
patients as compared to controls, while there was no significant difference
between PGN and IgAN patients and between IgAN and controls. CIE revealed a
pre-peak in 12 of 14 IgAN patients and a migration index which did not
differ between controls, IgAN, and PGN subjects. No pre-peak was observed
in PGN and in other glomerulonephritides. Analysis of plasma vWF multimeric
pattern by SDS- agarose gel electrophoresis disclosed in four IgAN patients
abnormally large vWF multimers that were not documented in PGN subjects.
CONCLUSIONS: This study, by showing the presence of a pre-peak and of large
vWF multimers in IgAN patients, suggests an altered postsecretory handling
of the vWF in IgAN and possibly a different role of the vWF in IgAN in
respect to PGN.
相似文献
49.