Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine

The nucleocapsid （N） protein of SARS-coronavirus （SARS-CoV） is the key protein for the formation of the helical nucleocapsid during virion assembly. This protein is believed to be more conserved than other proteins of the virus, such as spike and membrane glycoprotein. In this study, the N protein of SARS-CoV was expressed in Escherichia coli DHSalpha and identified with pooled sera from patients in the convalescence phase of SARS. A plasmid pCI-N, encoding the full-length N gene of SARS-CoV, was constructed. Expression of the N protein was observed in COS1 cells following transfection with pCI-N. The immune responses induced by intramuscular immunization with pCI-N were evaluated in a murine model. Serum anti-N immunoglobutins and splenocytes proliferative responses against N protein were observed in immunized BALB/c mice. The major immunoglobulin G subclass recognizing N protein was immunoglobulin G2a, and stimulated splenocytes secreted high levels of gamma interferon and IL-2 in response to N protein. More importantly, the immunized mice produced strong delayed-type hypersensitivity （DTH） and CD^8＋ CTL responses to N protein.     

不孕不育者对人工授精技术的伦理态度调查研究

目的：研究不孕不育者对人工授精技术的伦理态度,促进该项技术更好地为患者服务.方法：自制问卷调查了223名不孕不育者.结果：不孕不育者对AIH、AID的态度存在显著差异（P＜0.05）;对婚姻家庭生活选择相对宽容;精子的商品化趋向值得关注;必须采取一定措施以保证精子和人工授精儿的质量.结论：AID会引发许多伦理问题,临床应用时更应遵循有利于患者、知情同意等伦理原则和相关法律规定,以减少纠纷,造福社会.     

The up-regulation of preprodynorphin mRNA in trigeminoparabrachial neurons after inflammation

Inflammation of the temporomandibular joint (TMJ) produces an increase in preprodynorphin (PPD) mRNA expression in the spinal trigeminal nucleus (Vsp) and paratrigeminal nucleus (Pa5) of the rat. In this study, we further demonstrated that a portion of the TMJ inflammation-induced PPD mRNA positive neurons in the Vsp and Pa5 projected to the parabrachial nucleus (PB). In inflamed rats, the percentage of trigemino- and paratrigeminoparabrachial neurons with up-regulation of PPD mRNA was significantly increased in the ipsilateral Vsp (5.7+/-1.8%) and Pa5 (22.8+/-7.4%, n = 3) when compared with the contralateral side and with saline-treated controls (p < 0.05). These results suggest that the selective up-regulation of PPD mRNA in the Vsp and Pa5 following TMJ inflammation involves ascending trigeminal nociceptive pathways.     

Observations on the therapeutic effect of acupuncture plus tuina on retention of urine

将118例尿潴留患者随机分为2组,即针灸推拿治疗组和药物注射治疗组分别进行治疗观察.结果2组疗效有显著差异.并对2组治疗效果、治疗次数、疗效影响等比较分析,提示针灸推拿组显效率明显优于药物注射组.反映针灸配合推拿治疗尿潴留是临床一种较好的治疗方法.     

银翘解毒丸中金银花药材HPLC-UV鉴定及绿原酸含量测定

目的:建立银翘解毒丸(金银花,连翘,甘草,薄荷等)中金银花药材的鉴别和绿原酸含量测定的方法.方法:采用YWG C18色谱柱;甲醇-10%的磷酸氢二钠(冰醋酸调pH值为2.7)为流动相进行梯度洗脱;检测波长为326nm;流速为1.0mL·min-1;柱温为室温.结果:HPLC-UV法定性鉴别制剂中金银花药材;绿原酸在5.0～60μg·mL-1的范围内呈良好线性关系(r=0.9999),平均回收率为98.71%(RSD=1.57%).结论:本法操作简单,准确,重现性好,可用于银翘解毒丸的质量控制.     

Lnczc3h7a对结直肠癌细胞增殖和迁移的影响及其机制

目的 分析Lnczc3h7a在结直肠癌细胞中的表达及其对结肠癌细胞增殖和迁移的影响,并探讨其潜在的作用机制.方法 选择6种结直肠癌细胞株SW620、SW480、HCT116、DLD-1、Caco-2、HT-29与正常结直肠上皮细胞株FHC,采用RT-PCR法检测Lnczc3h7a在结直肠癌细胞株及正常结直肠上皮细胞株中...     

Determination of heavy metals in water using an FTO electrode modified with CeO2/rGO nanoribbons prepared by an electrochemical method

The rGO/CeO₂/FTO nanocomposite modified electrode was prepared by an electrochemical method. A simple and highly sensitive electrochemical sensing platform for electrochemical rGO and modified CeO₂ nanoribbons directly on FTO electrodes was developed. Simultaneous determination of Pb²⁺ and Cd²⁺ used the differential pulse anodic stripping voltammetry (DPASV) method. The method was simple to operate, and CeO₂ nanobelts could be obtained simultaneously by electrodeposition and reduction of GO without further processing. This is an environmentally friendly electrochemical method to obtain modified electrodes under mild conditions. The experimental results showed that the linear calibration curves of Pb²⁺ and Cd²⁺ are 1–300 and 0.2–500 μg L⁻¹, respectively. At the same time, no interference from other coexisting metal ions was found during the detection process, which proved that the modified electrode had good stability and repeatability.

The rGO/CeO₂/FTO nanocomposite modified electrode was prepared by an electrochemical method.     

Improved nitrogen reduction activity of NbSe2 tuned by edge chirality

Efficient catalysts for the electroreduction of N₂ to NH₃ are of paramount importance for sustainable ammonia production. Recently, it was reported that NbSe₂ nanosheets exhibit an excellent catalytic activity for nitrogen reduction under ambient conditions. However, existing theoretical calculations suggested an overpotential over 3.0 V, which is too high to interpret the experimental observations. To reveal the underlying mechanism of the high catalytic activity, in this work, we assessed NbSe₂ edges with different chirality and Se vacancies by using first principles calculations. Our results show that N₂ can be efficiently reduced to NH₃ on a pristine zigzag edge via the enzymatic pathway with an overpotential of 0.45 V. Electronic structure analysis demonstrates that the N₂ molecule is activated by the back-donation mechanism. The efficient tuning of the local chemical environments by edge chirality provides a promising approach for catalyst design.

The zigzag edge of the NbSe₂ monolayer exhibits an overpotential as low as 0.45 V along the enzymatic pathway.     

木犀草素对实验性自身免疫性脑脊髓炎大鼠SPH/SPHK1/S1P1通路及神经元凋亡的影响

目的 探讨木犀草素(Luteolin)对实验性自身免疫性脑脊髓炎(Experimental autoimmune en-cephalomyelitis,EAE)大鼠神经酰胺-鞘氨醇(Sphingosine,SPH)/鞘氨醇激酶1(Sphingosine kinase 1,SPHK1)/1-磷酸鞘氨醇受体1(Sphing...