兔动静脉短路环法诱导组织工程骨支架材料血管生成的初步研究

Objective To compare the effects of 2 vascular carriers, arteriovenous loop and arteri-ovenous bundle, on inducing angiogenesis in coral scaffold of vascularized tissue-engineered bone in animal models.Methods Thirty-six adult male New Zealand rabbits were randomized into 2 even groups.In group A, an arteriovenous loop (AVL) was formed by microsurgical anastomosis at the proximal ends between the femoral poptiteal artery and vein, and placed in the circular side groove of the coral block (6 mm × 8 mm × 10 mm) .In group B, flow-through vessels bundles of both femoral artery and vein were placed in the side grooves of the coral block.All the implants in 2 groups were wrapped by a micro-porous expand-ed-polytetrafluoroethylene (ePTFE) membrane, and fixed subcutaneously by suturing.Evaluation methods included gross morphological observations, histological examinations, India ink perfusion and vascular casting after 2, 4, 6 weeks.The density of blood vessels was analyzed by the statistical software SPSS 10.0.Results All the corals were encased by newly formed fibrovascular tissues in 2 groups.Ink-stained vessels distributed the surfaces and side grooves, and invaded the interspaces of corals.The degree of vascularization increased over the course of experiment.Blood vessel density demonstrated a significant continuous increase between 2 and 6 weeks after implantation in group A.The mean value of blood vessel density in group A (2 weeks 276.60±4.67, 4 weeks 517.20±10.66, 6 weeks 707.00 ±11.87) was significantly higher than in group B (2 weeks 153.60 ±7.16, 4 weeks 269.40±6.80, 6 weeks 279.20±6.53) (P <0.01).Vascular casting showed that in group A, significant blood vessels sprouted from all areas of the loop, espe-cially at the entrance of the arteriovenous pediele where the small tubes were densely interconnected.In group B, however, no blood vessels sprouted from the arteriovenous bundles and only some small vessels grew from the entrance and exit.Conclusions A vascularized coral model can be constructed by inserting an ar-teriovenous loop or an arteriovenous bundle, useful in vascular bone tissue engineering.The former, however, have stronger abilities to induce angiogenesis than the latter.     

自乳化释药系统的体外评价

目的 探讨自乳化释药系统的体外评价方法。方法 通过测定自乳化时间、乳化后乳剂粒径的大小及模型药物的体外溶出行为对自乳化释药系统进行体外评价。结果 优选出的自乳化处方10min内已基本乳化完全，乳化后乳剂粒子大小大多数在3μm左右，以吲哚美辛和尼莫地平为模型药物制备出自乳化释药系统，体外溶出结果表明：与混悬液相比，两种药物的体外溶出显提高。结论 自乳化释药系统能够提高难溶性药物的体外溶出。     

采用修正正交分段线性函数求解时滞系统和卷积的方法

应用修正正交分段线性函数的时滞和反向特性，推导出求解时滞系统和卷积的公式。数字仿真表明，它具有计算简单，精度高的优点。     

Mental stress-induced myocardial ischemia in coronary artery disease patients with left ventricular dysfunction

Background  Reduced left ventricular ejection fraction (LVEF) is a risk factor for poor outcomes in patients with coronary artery disease (CAD). Mental stress-induced myocardial ischemia (MSIMI) also identifies a subset of CAD patients at increased risk for future cardiovascular events. Susceptibility to MSIMI in patients with CAD and reduced LVEF is unknown. Methods and Results  We enrolled 182 patients (67 women) with a mean age of 64 years and a documented history of CAD in this study. Baseline resting ejection fraction was determined by use of technetium 99m sestamibi gated single photon emission computed tomography. Abnormal LVEF was defined as less than 45% for men and less than 50% for women (based on published norms for our software [Cedars-Sinai Medical Center]). All participants underwent mental stress testing with a public speaking task. Rest/stress myocardial perfusion single photon emission computed tomography was performed via conventional methodology. Images were visually compared for number and severity of perfusion defects by use of a scoring method from 0 to 4. A summed difference score was calculated as the difference between summed stress and rest scores. A score of greater than 3 was considered abnormal. MSIMI developed in 19% of patients with normal LVEF and 31% of those with reduced LVEF. There is no statistically significant difference between the two groups (P=.11). Conclusions  CAD patients with left ventricular dysfunction are equally susceptible to MSIMI as those with normal LVEF. This study was supported by grants HL 070265 and HL 072059 from the National Heart. Lung, and Blood Institute. This material is also the result of work supported by resources and with the use of facilities at the Department of Veterans. Affairs Medical Center, Gainesville, Fla.     

颈动脉支架置入术治疗颈动脉狭窄

颈动脉支架置入术(CS)作为一种治疗颈动脉狭窄(CAS)的新方法，与传统的颈动脉内膜剥离术相比较，具有易操作、创伤小、并发症低等优点，作者就CS的现状、操作方法及并发症处理作一综述。     

高压氧治疗对脑梗死患者血清基质金属蛋白酶-9的影响

目的探讨高压氧（HBO）治疗后急性脑梗死患者血清基质金属蛋白酶-9（MMP-9）水平的动态变化及其与临床疗效的关系。方法选取首次发病的24h以内的颈内动脉系统急性脑梗死患者76例，分为高压氧治疗组（HBO组，36例）和常规治疗组（常规组，40例）。常规组仅给予常规治疗，HBO组在常规治疗基础上加用HBO治疗。30例年龄、性别与脑梗死患者相匹配的健康人为健康对照组（正常对照组）。采用酶联免疫吸附双抗体夹心法测定脑梗死患者入院时、发病后第3、5、10天以及正常对照组血清MMP-9。所有患者于入院时和治疗后10d、1个月进行神经功能缺损评分，评价HBO组和常规组之间的疗效差异。结果（1）与正常对照组比较，HBO组和常规组入院时血清MMP-9的浓度均明显增高，差异有统计学意义（P〈0.01），但后二者之间差异无统计学意义（P〉0.05）。与治疗前相比，常规组和HBO组血清MMP-9水平均呈现先增高后降低的动态变化趋势，3d时达高峰，在治疗后3、5d时HBO组血清MMP-9浓度低于同期常规组（P〈0．05）。（2）治疗1个月后，神经功能缺失评分HBO组明显低于常规组；HBO组疗效优于常规组（P〈0．05）。（3）脑梗死患者神经功能缺损评分减少值与患者发病后第3天之血清MMP-9水平呈显著负相关。结论HBO治疗能降低脑梗死患者升高的血清MMP-9浓度，减轻神经功能缺失程度，其临床疗效可能与降低患者血清MMP-9的浓度有关。     

中西医结合治疗特发性面神经麻痹

自2003年7月-2005年2月，笔者采用中西医结合方法治疗特发性面神经麻痹32例，疗效显著，报告如下。     

再接再厉迎接我国炎症性肠病研究的新高潮——第二届全国炎症性肠病学术会议有感

2006年8月在广州召开的第二届全国炎症性肠病（IBD）学术会议检阅了2000年第一届全国会议以来该领域研究的丰硕成果．大会高质量的专题报告和论文交流。反映了我国近年IBD研究的新动向和新经验。会议由于有国外知名专家的参与、点评和樊代明、钟南山院士的报告、指示。更突显出其里程碑意义。回味这次学术会议的主要内容。对比近年来我们建议的研究策略。感触良多。深感形势喜人，发展趋势令人鼓舞。其中一些主要研究方向意义重大。需找准差距、明确目标。继续深入研究。才能赶上世界先进水平㈣。为此提出我们的意见，供同道参考。     

鼻内镜治疗颅底疾病112例报告

目的探讨鼻内镜径路治疗颅底疾病的价值。方法2002年5月~2006年12月,对112例颅底疾病行鼻内镜径路手术,其中脑脊液鼻漏39例,垂体瘤4例,脑膜膨出或脑膜脑膨出3例,蝶筛囊肿9例,蝶窦炎伴息肉2例,真菌性蝶窦炎12例,内翻性乳头状瘤11例,鼻咽纤维血管瘤6例,骨化纤维瘤2例,骨纤维异常增殖2例,脊索瘤7例,颅咽管瘤2例,鳞癌10例,未分化癌1例,乳头状瘤癌变2例。结果全组均在鼻内镜下处理,其中20例病变为次全切除,包括鳞癌8例、未分化癌1例、脊索瘤7例、颅咽管瘤2例、骨纤维异常增殖2例。脑脊液鼻漏1次手术成功31例(79.5%),2次成功4例,3次成功4例,最终成功率100%。1例脑膜脑膨出术后2个月复发并伴脑脊液漏,内镜下再次手术成功。1例内翻性乳头状瘤术后11个月复发,改行鼻侧切开术。1例真菌性蝶窦炎术后4个月复发行再次内镜手术。1例脑脊液鼻漏术后颅内感染。结论鼻内镜径路可以处理多种颅底疾病,是一种安全、有效、微创的手术方式,但仍需严格掌握手术适应证,特别是恶性肿瘤。     

高效液相色谱/质谱法检测人尿中的糖皮质激素及其它兴奋剂

采用液相色谱/质谱(LC/MS)方法检测糖皮质激素、甾体和β2激动剂。本文采用Zorbax 30 mm×2.1mm,1.8μm短柱,对12种糖皮质激素、3种甾体和2种β2激动剂的液相色谱/质谱的测定方法进行了研究,建立了6分钟内快速检测人尿中多种糖皮质激素、甾体和β2激动剂的测定方法。检测限低于5ng/ml。该方法适用于兴奋剂的筛选及确证。