Expression of interferon-alpha／beta receptor protein in liver of patients with hepatitis C virus-related chronic liver disease

ABM: To study the expression of interferon-alpha/beta (IFN-α/β) receptor protein in liver of patients with hepatitis C virus (HCV)-related chronic liver disease and its clinical significance. METHODS: A total of 181 patients with HCV-related chronic liver disease included 56 with HCV-related liver cirrhosis (LC) and 125 with chronic hepatitis C (CHC). CHC patients were treated with five megaunits of interferon-α1b six times weekly for the first 2 weeks and then every other day for 22 wk. The patients were divided into interferon (IFN) treatment-responsive and non-responsive groups, but 36 patients lost follow-up shortly after receiving the treatment. The expression of IFN-α/β receptor (IFN-α/βR) protein in liver of all patients was determined with immunofluorescence. RESULTS: In liver of patients with HCV-related chronic liver disease, the expression of IFN-α/βR protein in liver cell membrane was stronger than that in cytoplasm and more obvious in the surroundings of portal vein than in the surroundings of central vein. Moreover, it was poorly distributed in hepatic lobules. The weak positive, positive and strong positive expression of IFN-α/βR were 40% (50/125), 28% (35/125), 32% (40/125), respectively in CHC group, and 91.1% (51/56), 5.35% (3/56), and 3.56% (2/56), respectively in LC group. The positive and strong positive rates were higher in CHC group than in LC group (P<0.01). In IFN treatment responsive group, 27.8% (10/36) showed weak positive expression; 72.2% (26/36) showed positive or strong positive expression. In the non-responsive group, 71.7% (38/53) showed weak positive expression; 28.3% (15/53) showed positive or strong positive expression. The expression of IFN-α/βR protein in liver was more obvious in IFN treatment responsive group than in non-responsive group. CONCLUSION: Expression of IFN-α/βR protein in liver of patients with HCV-related chronic liver disease is likely involved in the response to IFN treatment.     

Characterization of the requirements for localization of phytochrome B to nuclear bodies

Phytochromes are red- and far-red-sensing photoreceptors that detect the quantity, quality, and duration of light throughout the entire life cycle of plants. Phytochromes accumulate in the cytoplasm in the dark. As one of the earliest responses after light illumination, phytochromes localize to the nucleus where they become associated with discrete nuclear bodies (NBs). Here, we describe the steady-state dynamics of Arabidopsis phytochrome B (phyB) localization in response to different light conditions and define four phyB subnuclear localization patterns: diffuse nuclear localization, small and numerous NBs only, both small and large NBs, and large NBs only. We show that phyB nuclear import is not sufficient for phyB NB formation. Rather, phyB accumulation in NBs is mainly determined by the percentage of the total amount of phyB protein that is in the active phyB conformer, with large NBs always correlating with strong phyB responses. A genetic screen to identify determinants required for subnuclear localization of phyB resulted in several phyB mutants, mutants deficient in phytochrome chromophore biosynthesis, and mutations in at least one previously uninvestigated locus. This study lays the groundwork for future investigations to identify the molecular mechanisms of light-regulated partitioning of plant photoreceptors to discrete subnuclear domains.     

急性冠状动脉综合征患者的CD4+CD28null T淋巴细胞电压门控性Kv1.3钾通道数量增加

目的 运用膜片钳技术检测急性冠状动脉综合征(ACS)患者的CD4+CD28nullT淋巴细胞Kv1.3钾通道的数量是否增加,探讨CD4+CD28nullT淋巴细胞上Kv1.3钾通道的表达.方法 选择17例ACS患者,11例年龄、性别匹配的正常体检者作为对照.运用荧光染色标记及膜片钳技术检测单个T淋巴细胞(CD4+CD28nullT淋巴细胞和CD4+CD28+T淋巴细胞)上Kv1.3钾通道的数量,比较Kv1.3通道在两种细胞上的差别.结果 ACS患者的CD4+CD28nullT淋巴细胞比例为(6.97±2.05)%,明显高于对照组的(1.38±0.84)%(P<0.05).ACS患者的CD4+CD28nullT淋巴细胞数量与hsCRP浓度呈正相关(r=0.52,P<0.05).ACS患者CD4+CD28nullT淋巴细胞Kv1.3通道的电导、密度、数量明显高于对照组CD4+CD28+T淋巴细胞(P<0.01).而两组CD4+CD28+T淋巴细胞间Kv1.3通道的电导、密度、数量差异无统计学意义(P>0.05).结论 ACS患者CD4+CD28nullT淋巴细胞明显增多.ACS患者CD4+CD28nullT淋巴细胞上Kv1.3钾通道数量比自身和对照的CD4+CD28+T淋巴细胞明显增多,CD4+CD28nullT淋巴细胞的功能可能与Kv1.3钾通道增多相关.     

Cloning and pharmacological characterization of a rat kappa opioid receptor.

A full-length cDNA was isolated from a rat striatal library by using low-stringency screening with two PCR fragments, one spanning transmembrane domains 3-6 of the mouse delta opioid receptor and the other unidentified but homologous to the mouse delta receptor from rat brain. The novel cDNA had a long open reading frame encoding a protein of 380 residues with 59% identity to the mouse delta receptor and topography consistent with a seven-helix guanine nucleotide-binding protein-coupled receptor. COS-1 cells transfected with the coding region of this clone showed high-affinity binding to kappa opioid receptor-selective ligands such as dynorphin A and U-50,488 and also nonselective opioid ligands such as bremazocine, ethylketocyclazocine, and naloxone. Not bound at all (or bound with low affinity) were dynorphin A-(2-13), enantiomers of naloxone and levophanol [i.e., (+)-naloxone and dextrorphan], and selective mu and delta opioid receptor ligands. Activation of the expressed receptor by kappa receptor agonists led to inhibition of cAMP. Finally, in situ hybridization revealed a mRNA distribution in rat brain that corresponded well to the distribution of binding sites labeled with kappa-selective ligands. These observations indicate that we have cloned a cDNA encoding a rat kappa receptor of the kappa 1 subtype.     

造血干细胞移植患者肠外营养支持的临床观察

目的探讨肠外营养（PN）支持对造血干细胞移植患者的重要性及各种营养素的作用。方法回顾性分析2008年1月至2009年9月106例接受造血干细胞移植患者的临床资料,根据营养支持方式不同分为PN组（n=42）和非PN组（n=64）,比较两组患者在造血重建时间、口腔黏膜炎、肝功能异常、感染及移植物抗宿主病（GVHD）发生率等方面的差异;进一步分析核黄素、谷氨酰胺、结构脂肪乳等营养素的作用。结果接受PN支持的42例患者中,包含接受核黄素的患者33例,谷氨酰胺24例,结构脂肪乳26例。PN组患者的口腔黏膜炎发生率显著低于非PN组（31．0％比51．6％,P=0．036）,而两组的感染率（42．9％比46．9％,P=0．898）、GVHD发生率（21．4％比31．3％,P=0．267）、丙氨酸氨基转移酶（ALT）水平[（49．5±9．8）U／L比（69．9±10．9）U／L,P=0．196]、粒系重建时间[（11．6±0．3）d比（12．3±0．3）d,P=0．187]及血小板重建时间[（14．1±0．9）d比（13．3±0．4）d,P=0．386]差异均无统计学意义。核黄素组的口腔黏膜炎发生率为27．3％,有低于无核黄素组（44．4％）和非PN组（51．6％）的趋势,但差异无统计学意义（P=0．073）。谷氨酰胺组、无谷氨酰胺组和非PN组在口腔黏膜炎发生率、感染率、GVHD发生率、ALT水平及造血重建时间方面的差异均无统计学意义（P均〉0．05）。结构脂肪乳组的ALT平均值为（38．7±4．9）U／L,有低于物理混合的中长链脂肪乳组[（68．5±23．0）U／L]和非PN组[（69．9±10．9）U／L]的趋势,但差异无统计学意义（P=0．243）。结论造血干细胞移植患者可从合理的PN中获益。核黄素对口腔黏膜炎的预防可能有益。结构脂肪乳与中长链脂肪乳对于肝脏功能具有同样的安全性。谷氨酰胺的作用有待进一步研究。     

上海口岸国际邮轮随船检疫工作模式初探

随着国家一系列支持政策的出台,短短几年间,我国邮轮经济已经从以国际邮轮入境接待为主的起步发展阶段,逐步进入国际邮轮出入境旅游并举的快速发展阶段,邮轮度假、邮轮旅游日益成为中国游客接受的新兴出游方式[1].同时,随着邮轮经济在中国的快速发展,上海作为国际航运中心的地位日益突出,邮轮行业在上海逐步深入,进入上海港的邮轮种类、行程安排越来越丰富多样,传统的靠泊检疫模式已无法完全满足检验检疫保障人员、船舶安全卫生的新需求,一种新型的邮轮随船检疫新模式日益得到检验检疫机构和邮轮相关行业的接收和认可.在国家质量监督检验检疫总局（以下简称国家质检总局）的大力支持和政策引导下,上海出入境检验检疫局（以下简称上海局）积极探索卫生检疫监管新模式,创造性的提出并推广了邮轮随船检疫这一适应新形势、新情况的邮轮检疫新手段,极大促进了上海的城市发展和地方经济的腾飞.     

医院行政总值班满意度分析

目的：了解医院行政总值班中存在的缺陷,分析原因和探讨其改进方法。方法发放调查表及查看有关会议记录,查看院外民众意见箱,回拨来电,询问医院行风社会监督员,调查资料采用SPSS 11．5进行统计分析。结果服务态度相关性缺陷率2．41％,业务技能相关性缺陷率23．37％,两大类缺陷率比较,存在明显差异（ P＜0．05）。结论医院行政总值班是保障医疗安全的重要防线和服务民众的重要窗口,应持续质量改进,不断提高值班人员的综合素质。     

去屑消风饮治疗颜面脂溢性皮炎疗效观察

目的：观察去屑消风饮治疗颜面脂溢性皮炎的临床疗效。方法：将60例符合纳入标准的肺胃热甚型面部脂溢性皮炎患者按照随机数字表法分为治疗组和对照组各30例,治疗组采用自拟方去屑消风饮口服治疗,对照组用维生素B6及甘草锌颗粒口服治疗,4周后观察并比较两组患者疗效。结果：治疗组在改善患者皮肤瘙痒、油腻、脱屑等方面的疗效均明显优于对照组,且治疗组总有效率93.33%明显高于对照组的46.67%,比较差异均有统计学意义（P〈0.05）。结论：自拟方去屑消风饮治疗颜面脂溢性皮炎肺胃热甚型疗效显著,值得临床推广。