全文获取类型
收费全文 | 199773篇 |
免费 | 2267篇 |
国内免费 | 70篇 |
专业分类
耳鼻咽喉 | 1490篇 |
儿科学 | 8430篇 |
妇产科学 | 3581篇 |
基础医学 | 19320篇 |
口腔科学 | 1994篇 |
临床医学 | 13708篇 |
内科学 | 35238篇 |
皮肤病学 | 1655篇 |
神经病学 | 17477篇 |
特种医学 | 9814篇 |
外科学 | 32406篇 |
综合类 | 3233篇 |
一般理论 | 10篇 |
预防医学 | 19285篇 |
眼科学 | 4072篇 |
药学 | 11524篇 |
中国医学 | 772篇 |
肿瘤学 | 18101篇 |
出版年
2023年 | 168篇 |
2022年 | 449篇 |
2021年 | 823篇 |
2020年 | 445篇 |
2019年 | 540篇 |
2018年 | 22498篇 |
2017年 | 17714篇 |
2016年 | 20067篇 |
2015年 | 1504篇 |
2014年 | 1738篇 |
2013年 | 1845篇 |
2012年 | 8399篇 |
2011年 | 22475篇 |
2010年 | 19572篇 |
2009年 | 12122篇 |
2008年 | 20563篇 |
2007年 | 22824篇 |
2006年 | 1582篇 |
2005年 | 3145篇 |
2004年 | 4235篇 |
2003年 | 5145篇 |
2002年 | 3211篇 |
2001年 | 974篇 |
2000年 | 1077篇 |
1999年 | 744篇 |
1998年 | 435篇 |
1997年 | 374篇 |
1996年 | 227篇 |
1995年 | 231篇 |
1994年 | 206篇 |
1993年 | 185篇 |
1992年 | 389篇 |
1991年 | 471篇 |
1990年 | 461篇 |
1989年 | 431篇 |
1988年 | 358篇 |
1987年 | 314篇 |
1986年 | 289篇 |
1985年 | 307篇 |
1984年 | 237篇 |
1983年 | 201篇 |
1980年 | 137篇 |
1979年 | 243篇 |
1978年 | 166篇 |
1977年 | 151篇 |
1976年 | 143篇 |
1975年 | 168篇 |
1974年 | 173篇 |
1973年 | 177篇 |
1972年 | 138篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
171.
Lijun Di Jun Ren Ying Yan 《中德临床肿瘤学杂志》2007,6(4):393-395
Objective: To evaluate the effect and safety of clinical use of zoledronic acid in the treatment of malignant hypercalcemia. Methods: A multi-center, open phase II clinical trial was conducted in 15 cases with malignant hypercalcemia who received zoledronic acid intravenously for 15 min. The level of blood calcium and side effects were recorded regularly within 28 days after injection. Results: One case was dropped out due to bad compliance. The complete response rate (the corrected serum calcium was reduced to normal level) was 100.00% (14/14). The medium time of complete response rate was 5.07 days. The medium maintain time was 22.30 days. Slight, or moderate fever was observed. Conclusion: Zoledronic acid can effectively reduce the malignant hypercalcemia. The use of zoledronic acid appears to be safety and convenient. 相似文献
172.
173.
Differentiation of pancreatic epithelial progenitor cells into hepatocytes following transplantation into rat liver 下载免费PDF全文
Mariana D. Dabeva Seong-Gyu Hwang Srinivasa Rao G. Vasa Ethel Hurston Phyllis M. Novikoff Douglas C. Hixson Sanjeev Gupta David A. Shafritz 《Proceedings of the National Academy of Sciences of the United States of America》1997,94(14):7356-7361
The ability to identify, isolate, and transplant progenitor cells from solid tissues would greatly facilitate the treatment of diseases currently requiring whole organ transplantation. In this study, cell fractions enriched in candidate epithelial progenitor cells from the rat pancreas were isolated and transplanted into the liver of an inbred strain of Fischer rats. Using a dipeptidyl dipeptidase IV genetic marker system to follow the fate of transplanted cells in conjunction with albumin gene expression, we provide conclusive evidence that, after transplantation to the liver, epithelial progenitor cells from the pancreas differentiate into hepatocytes, express liver-specific proteins, and become fully integrated into the liver parenchymal structure. These studies demonstrate the presence of multipotent progenitor cells in the adult pancreas and establish a role for the liver microenvironment in the terminal differentiation of epithelial cells of foregut origin. They further suggest that such progenitor cells might be useful in studies of organ repopulation following acute or chronic liver injury. 相似文献
174.
This study demonstrates that ZnSO4 induced chemical trauma results in an in situ regeneration of the olfactory epithelium which, when maintained in vitro, provides an enriched population of olfactory neurons. Therefore, the ability of the olfactory epithelium to respond to chemical trauma with increased mitotic activity can be used to increase growth of neurons in culture. Tissue obtained from normal or vehicle-treated adult mice produced few olfactory neurons, when maintained in culture, compared to cultures established from tissue following an in situ ZnSO4 trauma. Maximal neuronal yields were obtained in cultures established from tissue that was removed 4–6 days following chemical trauma. The morphological appearance and the presence of cell specific intermediate filament proteins were used to classify the cell types in these olfactory epithelial cultures. Single cells and aggregates of cells which were immunopositive for keratin, but immunonegative for neurofilament protein and GFAP, were identified as epithelioid. Flattened polygonal cells immunopositive for GFAP were identified as glia. A small population of flattened cells was immunonegative for all of the antibodies used in this study. Cells that had processes were immunonegative for GFAP and keratin. Some were immunopositive for 200 kDa and 160 kDa neurofilament proteins but immunonegative for the 68 kDa neurofilament protein. A few of these cells showed positive immunoreactivity with the olfactory marker protein (OMP) antibody and most likely represented the most mature olfactory neurons in the cultures. This trauma-induced culture model using olfactory tissue from adult mice can serve as a source of CNS neurons for comparison with cultured embryonic neurons. 相似文献
175.
The optimal conditions for inactivation of Bordetella pertussis organisms with glutaraldehyde for the production of a safe and potent whole cell pertussis vaccine were investigated. Two bacterial harvests from B. pertussis strain 10536 were treated with glutaraldehyde, each with 0.025, 0.05 and 0.1% concentrations of glutaraldehyde for 10, 60 and 120 min. The nine types of glutaraldehyde-inactivated pertussis vaccine (GIPV) and conventional heat-inactivated pertussis vaccine (HIPV) preparations made from two bacterial harvests were comparatively evaluated for the mouse weight gain test (MWGT), potency, and the histamine-sensitization (HS) and leucocytosis-promoting-factor (LPF) tests. The minimum period for killing the B. pertussis organisms with glutaraldehyde was>10 min for 0.025%, 10 min for 0.05% and 5 min for 0.1% concentration. The average loss in opacity varied from 5 to 10% for GIPV preparations and was 14% for HIPV preparations. The GIPV preparations except those inactivated with 0.025% glutaraldehyde for 10 min (GIPV-A) were much less toxic than the HIPV preparations in the MWGT. The GIPV-A preparations did not pass the MWGT. The GIPV preparations were also much less toxic in HS and LPF tests than the HIPV preparation. The potency of GIPV preparations inactivated with 0.05% glutaraldehyde for 10 min (GIPV-D) was similar to that of HIPV preparations. The prolonged treatments with glutaraldehyde reduced the potency. The GIPV-D preparation with good potency and less toxicity was found to be inactivated with glutaraldehyde under optimal conditions. All the preparations were innocuous in the abnormal toxicity test. 相似文献
176.
A L Usala I Wexler A Posch M K Gupta 《American journal of diseases of children (1960)》1992,146(9):1074-1077
OBJECTIVE--To determine the course of maternally derived elevations in thyrotropin-binding inhibitory immunoglobulins in a neonate. DESIGN--Case report. SETTING--University pediatric endocrinology clinic and endocrine immunology laboratory in Ohio. PARTICIPANTS--An infant with elevated thyrotropin levels but near-normal total thyroxine levels, and her mother. INTERVENTIONS--None. MEASUREMENTS/MAIN RESULTS--Thyroid hormone, thyrotropin, and thyrotropin-blocking immunoglobulin concentrations were serially measured in a woman and her infant, who was found to have elevated thyrotropin levels (234 mU/L) and borderline low thyroxine levels (95 nmol/L). As infant thyroxine concentrations remained normal (125 to 145 nmol/L), no thyroxine supplementation was given. Thyrotropin levels decreased concomitantly with thyrotropin-blocking inhibitory immunoglobulin levels, and normalized by day 56 of life. The apparent elimination half-life of thyrotropin-blocking immunoglobulins was 7.5 days. CONCLUSIONS--The observed parallel elimination kinetics suggest that the thyrotropin receptor antibody acts as a thyrotropin antagonist, resulting in compensatory thyrotropin elevations. The duration of such elevations may be predicted on the basis of such elimination. 相似文献
177.
178.
179.
R J Crawford A Gupta G Risitano F D Burke 《The Journal of hand surgery, European volume》1990,15(1):113-114
Thirty-five patients who had thirty-seven mucous cysts excised from the distal interphalangeal joints were reviewed not less than one year later. Seven out of 25 which had been treated by simple excision recurred, whereas only one out of twelve treated by excision and skin closure with a rotation flap recurred. 相似文献
180.
Yuichi Sanada Kazuhiro Yoshida Hiroyuki Itoh Satoko Kunita Kazuto Jinushi Hideo Matsuura 《Journal of hepato-biliary-pancreatic sciences》2007,14(4):401-409
We report a case of groove pancreatitis (GP) associated with a true pancreatic cyst. An 81-year-old man who had suffered epigastric pain for 4 months was referred to Saisekai Kure Hospital. Computed tomography and endoscopic retrograde pancreatography showed a cystic lesion in the groove area of the pancreas. Serum amylase elevation and imaging findings suggested GP due to the cyst. Six weeks of medical treatment did not improve the clinical symptoms. Therefore, pancreatoduodenectomy was performed. Histologic examination revealed a true cyst with intraluminal necrosis, which produced a protein plug that obstructed the Santorini duct. The parenchyma surrounding the groove area showed marked fibrosis and inflammatory cell infiltration. GP due to true pancreatic cyst was diagnosed. Although GP is usually caused by overconsumption of alcohol, which leads to changes in the pancreatic juice and the ultimate blockage of pancreatic outflow, the histologic features in our patient suggest that true pancreatic cyst stands as a secondary cause of GP. 相似文献