全文获取类型
收费全文 | 4084篇 |
免费 | 341篇 |
国内免费 | 7篇 |
专业分类
耳鼻咽喉 | 29篇 |
儿科学 | 90篇 |
妇产科学 | 53篇 |
基础医学 | 633篇 |
口腔科学 | 41篇 |
临床医学 | 546篇 |
内科学 | 982篇 |
皮肤病学 | 140篇 |
神经病学 | 255篇 |
特种医学 | 82篇 |
外科学 | 317篇 |
综合类 | 29篇 |
现状与发展 | 1篇 |
一般理论 | 1篇 |
预防医学 | 440篇 |
眼科学 | 63篇 |
药学 | 434篇 |
中国医学 | 33篇 |
肿瘤学 | 263篇 |
出版年
2024年 | 7篇 |
2023年 | 59篇 |
2022年 | 187篇 |
2021年 | 283篇 |
2020年 | 161篇 |
2019年 | 168篇 |
2018年 | 151篇 |
2017年 | 110篇 |
2016年 | 132篇 |
2015年 | 132篇 |
2014年 | 140篇 |
2013年 | 179篇 |
2012年 | 295篇 |
2011年 | 257篇 |
2010年 | 171篇 |
2009年 | 136篇 |
2008年 | 228篇 |
2007年 | 238篇 |
2006年 | 190篇 |
2005年 | 199篇 |
2004年 | 152篇 |
2003年 | 158篇 |
2002年 | 117篇 |
2001年 | 89篇 |
2000年 | 67篇 |
1999年 | 60篇 |
1998年 | 26篇 |
1997年 | 12篇 |
1996年 | 12篇 |
1995年 | 12篇 |
1994年 | 12篇 |
1993年 | 13篇 |
1992年 | 29篇 |
1991年 | 21篇 |
1990年 | 26篇 |
1989年 | 22篇 |
1988年 | 26篇 |
1987年 | 19篇 |
1986年 | 11篇 |
1985年 | 21篇 |
1984年 | 12篇 |
1983年 | 12篇 |
1982年 | 10篇 |
1981年 | 7篇 |
1980年 | 6篇 |
1979年 | 7篇 |
1978年 | 6篇 |
1969年 | 7篇 |
1967年 | 7篇 |
1966年 | 6篇 |
排序方式: 共有4432条查询结果,搜索用时 31 毫秒
21.
Role of Eotaxin-1 (CCL11) and CC chemokine receptor 3 (CCR3) in bleomycin-induced lung injury and fibrosis
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Huaux F Gharaee-Kermani M Liu T Morel V McGarry B Ullenbruch M Kunkel SL Wang J Xing Z Phan SH 《The American journal of pathology》2005,167(6):1485-1496
Eotaxin-1/CCL11 and its receptor CCR3 are involved in recruitment of eosinophils to diverse tissues, but their role in eosinophil recruitment in pulmonary fibrosis is unclear. The present study examined the pulmonary expression of CCL11 and CCR3 during bleomycin (blm)-induced lung injury and determined their importance in the recruitment of inflammatory cells and the development of lung fibrosis. In mice, blm induced a marked pulmonary expression of CCL11 and CCR3. Immunostaining for CCR3 revealed that this receptor was not only expressed by eosinophils but also by neutrophils. CCL11-deficient (CCL11(-/-)) mice developed significantly reduced pulmonary fibrosis. Expression of profibrotic cytokines such as transforming growth factor-beta1 was diminished in the absence of CCL11. Furthermore, increased lung expression of CCL11 significantly enhanced blm-induced lung fibrosis and production of profibrotic cytokines. These effects were also associated with an increase of eosinophil and neutrophil pulmonary infiltration. In contrast, mice treated with neutralizing CCR3 antibodies developed significantly reduced pulmonary fibrosis, eosinophilia, neutrophilia, and expression of profibrotic cytokines. Together, these data suggest that CCL11 and CCR3 are important in the pulmonary recruitment of granulocytes and play significant pathogenic roles in blm-induced lung fibrosis. 相似文献
22.
23.
M A Bach L Chatenoud D Wallach F Phan Dinh Tuy F Cottenot 《Clinical and experimental immunology》1981,44(3):491-500
T cell subsets and T cell functions were explored in 31 leprosy patients with the following methods: determination of the percentages of the different T cell subpopulations defined by monoclonal antibodies directed at total T cells, helper T cells and suppressor/cytotoxic T cells; measurement of the in vitro proliferative responses to mitogens; study of the concanavalin A-induced suppressive activity, assessed on MLC; measurement of delayed-type hypersensitivity by skin testing. The confrontation between immunological lepromatous patients without type-2 reaction (erythema nodosum leprosum), (2) lepromatous patients without ENL (erythema nodosum leprosum), (2) lepromatous patients was recent ENL and (3) tuberculoid patients. Unexpectedly, groups 1 and 3, although differing strongly in their clinical status and their sensitivity to lepromin (absent in group 1 and strong in group 3), showed a similar immunological profile with a normal percentage of T cells and a normal distribution of T cells among the major T cell subset contrasting with a moderate decrease of proliferative responses to mitogens and impaired delayed-type hypersensitivity reactions. Concanavalin A-induced suppressive activity was type-2 reaction) strongly differed from both other groups, showing striking abnormalities other groups, showing striking abnormalities of the repartition of the T cell subsets, with increased percentages of helper T cells and decreased percentages of suppressor T cells, and elevated proliferative responses to mitogens. Concanavalin A-induced suppressive activity was reduced in most patients of this group. It is suggested that this imbalance between T cell subsets contributes to the occurrence of ENL reactions in lepromatous patients. 相似文献
24.
J. Tamarelle A.C.M. Thiébaut B. de Barbeyrac C. Bébéar J. Ravel E. Delarocque-Astagneau 《Clinical microbiology and infection》2019,25(1):35-47
Background
The vaginal microbiota may modulate susceptibility to human papillomavirus (HPV), Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium infections. Persistent infection with a carcinogenic HPV is a prerequisite for cervical cancer, and C. trachomatis, N. gonorrheae and M. genitalium genital infections are all associated with pelvic inflammatory disease and subsequent infertility issues.Objectives
To evaluate the association between these infections and the vaginal microbiota.Data sources
The search was conducted on Medline and the Web of Science for articles published between 2000 and 2016.Study eligibility criteria
Inclusion criteria included a measure of association for vaginal microbiota and one of the considered STIs, female population, cohort, cross-sectional and interventional designs, and the use of PCR methods for pathogen detection.Methods
The vaginal microbiota was dichotomized into high-Lactobacillus vaginal microbiota (HL-VMB) and low-Lactobacillus vaginal microbiota (LL-VMB), using either Nugent score, Amsel's criteria, presence of clue cells or gene sequencing. A random effects model assuming heterogeneity among the studies was used for each STI considered.Results
The search yielded 1054 articles, of which 39 met the inclusion criteria. Measures of association with LL-VMB ranged from 0.6 (95% CI 0.3–1.2) to 2.8 (95% CI 0.3–28.0), 0.7 (95% CI 0.4–1.2) to 5.2 (95% CI 1.9–14.8), 0.8 (95% CI 0.5–1.4) to 3.8 (95% CI 0.4–36.2) and 0.4 (95% CI 0.1–1.5) to 6.1 (95% CI 2.0–18.5) for HPV, C. trachomatis, N. gonorrhoeae and M. genitalium infections, respectively.Conclusions
Although no clear trend for N. gonorrhoeae and M. genitalium infections could be detected, our results support a protective role of HL-VMB for HPV and C. trachomatis. Overall, these findings advocate for the use of high-resolution characterization methods for the vaginal microbiota and the need for longitudinal studies to lay the foundation for its integration in prevention and treatment strategies. 相似文献25.
Linda Wittkop Daniel Commenges Isabelle Pellegrin Dominique Breilh Didier Neau Denis Lacoste Jean-Luc Pellegrin Geneviève Chêne François Dabis Rodolphe Thiébaut 《BMC medical research methodology》2008,8(1):68
Background
Principal component analysis (PCA) and partial least square (PLS) regression may be useful to summarize the HIV genotypic information. Without pre-selection each mutation presented in at least one patient is considered with a different weight. We compared these two strategies with the construction of a usual genotypic score. 相似文献26.
Type I collagen gene expression in human atherosclerosis. Localization to specific plaque regions. 总被引:4,自引:2,他引:4
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
M. D. Rekhter K. Zhang A. S. Narayanan S. Phan M. A. Schork D. Gordon 《The American journal of pathology》1993,143(6):1634-1648
Because collagen is a major component of the human atherosclerotic plaque, factors controlling collagen synthesis may have a profound influence on the volume growth of these intimal lesions. In human arteries, we compared normal vs atherosclerotic media vs intimas for type I collagen gene expression using immunocytochemistry and in situ messenger RNA hybridization with subsequent correlations with plaque topographical features. We also determined the associations of such collagen gene expression with proximity to monocyte/macrophages and T lymphocytes. Type I collagen synthesis appears to be upregulated in atherosclerotic plaques compared with their underlying medias and normal internal mammary arteries and coronary diffuse intimal thickenings. At least in established and advanced coronary and carotid plaques, type I collagen gene expression is focal and especially prevalent in fibrous cap and vascularized regions. Although macrophages and type I procollagen messenger RNA and protein are both found in atherosclerotic plaques, no apparent spatial correlation between macrophage presence and type I procollagen presence was found within these atherosclerotic intimas. Type I procollagen presence appears to be negatively associated with the spatial presence of T cells. Thus, human atherosclerotic plaques exhibit nonuniform patterns of type I collagen gene expression. Although the biochemical determinants of this focal gene expression have yet to be determined, it is conceivable that stimulatory/inhibitory cytokines and other factors (eg hemodynamics) play important roles in determining the focal nature of collagen synthesis in atherosclerosis. 相似文献
27.
Chronic diarrhea,hemorrhagic colitis,and hemolytic-uremic syndrome associated with HEp-2 adherent Escherichia coli in adults infected with human immunodeficiency virus in Bangui,Central African Republic
下载免费PDF全文
![点击此处可从《Journal of clinical microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Mossoro C Glaziou P Yassibanda S Lan NT Bekondi C Minssart P Bernier C Le Bouguénec C Germani Y 《Journal of clinical microbiology》2002,40(8):3086-3088
In human immunodeficiency virus (HIV)-infected adults from the Central African Republic, the occurrence of chronic diarrhea due to HEp-2 adherent Escherichia coli (EAEC) harboring virulence markers (eaeA, BFP, EAF, astA determinant of EAST/1, positive FAS test, enteropathogenic E. coli O serogroup) was shown to be associated with AIDS. We also show that EAEC that produce verotoxin (Stx2) but do not harbor the genetic markers for classical enterohemorrhagic E. coli are involved in hemorrhagic colitis and hemolytic-uremic syndrome in patients with HIV. 相似文献
28.
29.
Cary-Blair, a transport medium for Vibrio parahemolyticus 总被引:2,自引:0,他引:2
30.
Lai PS Takeshima Y Adachi K Van Tran K Nguyen HT Low PS Matsuo M 《Journal of human genetics》2002,47(10):0552-0555
The frequency and distribution of deletions of 19 deletion-prone exons clustered in two hot spots in the proximal and central
regions of the dystrophin gene were compared in three populations from Singaporean, Japan, and Vietnam. DNA samples obtained
from 105 Singaporean, 86 Japanese, and 34 Vietnamese Duchenne muscular dystrophy patients were examined by polymerase chain
reaction amplification. Deletions of the examined exons were found in 51.2% of Japanese patients but in 40.0% or less of the
Singaporeans and Vietnamese. About two thirds of the deletions were localized in the central region and the remaining deletions
were clustered at the proximal region. The most commonly deleted exons at the central deletion hot spot were exon 50 in the
Singaporean, exons 49 and 50 in the Japanese, and exon 51 in the Vietnamese population. At the proximal deletion hot spot,
the most commonly deleted exons were exons 6 and 8 in the Singaporeans, exons 12 and 17 in the Japanese, and exons 8 and 12
in the Vietnamese. Two cases each from Singapore and Japan had large-scale gross mutations spanning both deletion hot spots.
Our results suggest that, although the presence and frequency of the two deletion hot spots may be similar in the three Asian
populations analyzed, the distribution and frequency of deletions among the different exons can vary as a result of population-specific
intronic sequences that predispose individuals to preferential deletion breakpoints.
Received: May 20, 2002 / Accepted: July 1, 2002 相似文献