伊曲康唑联合吡美莫司治疗头面部脂溢性皮炎的效果观察及其对皮肤生理参数和炎性因子水平的影响

目的：观察伊曲康唑联合吡美莫司治疗头面部脂溢性皮炎伴糠孢子菌感染的疗效及其对皮肤生理参数和炎性因子水平的影响。方法将68例脂溢性皮炎伴糠孢子菌感染患者随机分为对照组和研究组,每组34例。对照组患者给予伊曲康唑胶囊口服,并外用2％酮康唑洗剂,疗程4周;研究组患者在对照组治疗的基础上加用1％吡美莫司乳膏外用,疗程4周。比较两组患者的临床疗效、皮肤生理参数和炎性因子水平的变化。结果研究组患者的有效率为88．24％,高于对照组的61．76％（P＜0．05）;研究组的真菌镜检阴性率高于对照组,复发率低于对照组（P＜0．05）;但两组患者不良反应发生率比较差异无统计学意义（ P＞0．05）。治疗前两组患者皮肤含水量、白介素-10、经表皮失水量、皮脂分泌率、γ-干扰素和肿瘤坏死因子-α水平差异均无统计学意义（P＞0．05）,治疗后,观察组上述指标优于对照组,差异有统计学意义（P＜0．05）。两组患者治疗后皮质醇水平、pH值比较无差异（P＞0．05）。结论伊曲康唑联合吡美莫司可显著提高头面部脂溢性皮炎伴糠孢子菌感染的治疗效果,增加皮肤含水量,降低油脂分泌和炎性因子水平。     

急性脑血管病与心肌酶谱改变相关性分析

目的探讨急性脑血管病与心肌酶谱改变相关性.方法对206例急性脑血管患者的以下情况进行回顾性调查:血清心肌酶谱,性别,年龄,神志,偏瘫等级,既往有无冠心病史,中医病理因素风、火、痰、瘀、虚,脑出血,脑梗塞,心电图改变,有无脑水肿、肺部感染、消化道出血等严重并发症,预后;并将调研资料经数量化处理输入计算机,应用统计学处理软件SPSS 11.0,进行急性脑血管病诸因素与血清心肌酶谱异常的相关性分析.结果急性脑血管病患者血清心肌酶升高与脑出血呈正相关(r=0.185,P<0.05),与中医病理因素火热呈正相关(r=0.215,P<0.05),与意识障碍呈显著性正相关(r=0.256,P<0.001),与并发症的发生呈显著性正相关(r=0.251,P<0.001),与死亡呈显著性正相关(r=0.312,P<0.001),与中医病理因素风呈负相关(r=-0.139,P<0.05),与中医病理因素瘀呈负相关(r=-0.174,P<0.05),与年龄、偏瘫等级、既往有无冠心病史及中医病理因素痰、虚以及脑梗塞、心电图改变无相关.结论急性脑血管病尤其脑出血伴意识障碍患者血清心肌酶谱容易升高且预后较差.     

Smad7基因沉默对细胞增殖的影响

TGF—β是一种多功能的细胞因子,可调节细胞的分化、增殖和凋亡。它对多种上皮细胞尤其是支气管上皮细胞的生长、增殖有很强的抑制作用。Smad7作为TGF—β信号转导通路的抑制分子,可以抑制TGF—β信号在胞浆内的传导。我们以永生化人支气管上皮细胞BEP2D,及恶性转化的BERP35T2细胞为基础所做的研究表明：Smad7基因的表达随细胞恶性度增高而增高,     

重组E.coli. LLO/OVA明显增强小鼠CD11c细胞活性及抗肿瘤免疫

目的 探讨重组E.coli. LLO/OVA 诱导C57BL/6小鼠机体免疫的途径,观察其免疫后小鼠机体抑制B16-OVA黑色素瘤的效果.方法 磁珠分离E.coli. LLO/OVA及E.coli. OVA免疫后小鼠脾脏CD11c、CD4 和CD8 T细胞并检测CD11c细胞诱导同源CD4 和CD8 T增殖水平和细胞因子分泌程度;流式细胞检测小鼠脾脏内肿瘤抗原OVA257-264 SIINFEKL特异的细胞毒T细胞含量.比较两种E.coli. 免疫后,恶性黑色素瘤B16-OVA在小鼠肺内形成瘤结节的数量.结果 与E.coli. OVA相比, E.coli. LLO/OVA免疫后小鼠脾脏CD11c细胞诱导同源CD4 T细胞增殖作用增强、IL-2分泌增高;同时诱导CD8 T细胞增殖和IFN-γ分泌的作用也明显增强;OVA257-264 SIINFEKL特异的CD8 T细胞含量也明显增高;小鼠肺内形成B16-OVA瘤结节平均数明显减少. 结论 E.coli. LLO/OVA有效地诱导小鼠CD11c细胞活化,增强其对CD4 T细胞增殖和IL-2分泌以及对CD8 T细胞增殖、IFN-γ分泌的作用,诱导了更多的OVA特异的CD8 T细胞,使机体产生了更强的抗肿瘤免疫.     

刺壳花椒 HPLC 指纹图谱的研究

目的 采用高效液相色谱法研究并建立了产于张家界的刺壳花椒药材的指纹图谱。方法 Diamonsil C₁₈ 色谱柱 (250 mm×4.6 mm, 5 μm),乙腈-水流动相系统梯度洗脱,体积流量 1.0 mL/min,检测波长 278 nm。结果 对所采集的10批刺壳花椒药材进行了测定,建立了刺壳花椒的指纹图谱,共有11个共有峰。结论 HPLC 法色谱指纹图谱分析技术可用于刺壳花椒药材质量的控制。     

穴位埋线对血管性痴呆大鼠学习记忆能力和Bcl-2表达的影响

目的: 探讨穴位埋线对血管性痴呆(VD)大鼠学习记忆的影响及可能的机制.方法: 改良Pulsinelli's四血管阻断法建立血管性痴呆大鼠模型.造模后给予穴位埋线,用水迷宫检测学习记忆能力,免疫组织化学观察海马Bcl-2的表达.结果: 穴位埋线后VD大鼠学习记忆能力明显提高,海马齿状回Bcl-2表达增强.结论: 穴位埋线改善VD大鼠的学习记忆能力,可能与上调海马Bcl-2的表达、保护缺血后海马神经元有关.     

Reduction of RhoC is involved in the decreased migration of neural stem/precursor cells

OBJECTIVE Alzheimer′s disease(AD) is mainly characterized by a progressive loss of neurons and the deposition of beta-amyloid peptides(Aβ).It was demonstrated that transplanted and endogenous neural stem cells or neural precursor cells can survive,migrate,and differentiate into neurons.Previously we found that Aβ42 could disturb the migratory ability of neural stem/precursor cells.We therefore hypothesized that Aβ42 may affect some important proteins through Rho pathway and result in the decreased migration of neural stem/precursor cells.METHODS AND RESULTS We applied siRNA technology to knock down the expression of RhoC in neural stem/precursor cells and found that the expression of RhoC was down-regulated;Trans well assay was used to measure the migratory ability of the neural stem/precursor cells and the result showed that the migratory ability was disturbed when the expression of RhoC was down-regulated;WRW4 was used for inhibiting the effects of the Aβ42 through the FPRL1.CONCLUSION The reduction of RhoC was involved in the decreased migration of neural stem cells.     

The long-term outcomes of persistent childhood allergic asthma: A cross-sectional study from western Anatolia: Childhood persistent asthma in western Anatolia

BackgroundProspective cohort studies have provided useful knowledge about the natural history of asthma. However, most of the studies are conducted in western countries but the course of the disease and long-term outcomes may differ between countries due to environmental and cultural factors.ObjectiveThe aim of this study is to describe the long-term outcomes of childhood asthma, with data from a follow-up study of at least 10 years, in western Anatolia, Turkey.MethodsFifty-two patients diagnosed with persistent allergic asthma participated in the study. The patient's demographics, findings on admission, age at onset of disease, time of diagnosis, history of other allergic conditions, history of parental asthma and allergic disorders, presence of pharmacotherapy and immunotherapy were obtained from patients’ records. The factors influencing remission at the end of 10 years follow-up were evaluated.ResultsA total of 20 patients (38.5%) were on remission at the end of 10 years. The type of allergen, multi-allergen sensitivity, eosinophilia and elevated serum immunoglobulin E on admission, accompanying allergic disorders and atopy in parents, and allergen immunotherapy did not affect the remission rate (p > 0.05).ConclusionChildhood persistent asthma is not a homogeneous clinical entity but high clinical remission rates are obtained in western Anatolia. There is no significant predictor of clinical remission in long term follow-up. Prospective studies should be performed in larger asthmatic populations to obtain further data about the natural course of childhood asthma.     

大鼠心肌缺血预适应延迟相的差异蛋白质的分离及鉴定

目的采用蛋白质组学技术探讨缺血预适应心肌内源性保护的分子机制。方法应用双向凝胶电泳技术分离假手术组和心肌缺血预适应延迟相组大鼠的心肌总蛋白质。结果在假手术组的双向凝胶电泳图谱上展示704±27个蛋白质点,而在心肌缺血预适应延迟相组展示778±35个蛋白质点。经凝胶图像分析找到的差异蛋白质23个,基质辅助激光解析—电离飞行时间质谱对差异蛋白进行鉴定,共鉴定出12个蛋白质。这些蛋白质包括应激反应蛋白、代谢相关蛋白、信号调节蛋白、结构蛋白等。结论这些差异蛋白可能通过其分子伴侣功能、清除自由基、改善能量代谢等发挥心肌缺血预适应延迟相的心肌保护作用。     

中美临床执业医师资格考试中关于医学人文考核的比较研究

医师资格考试是大多数国家普遍采用的医师资格认证形式。医学人文的考核在医师资格考试中占有重要地位。以临床类别考试为例,通过文献分析、比较研究的方法,比较分析中美两国执业医师资格考试和医师资格分阶段考试实证研究考试中的医学人文考核,包括考试内容和形式、考试分值及比例。发现两国医学人文考核内容和比例基本相当,但我国现行考试医学人文考核站数较少,考试形式和呈现方式有待进一步改善,下一步应继续探索标准化病人应用于我国医师资格考试的可行性,充分发挥医师资格考试对医学教育的导向作用。