全文获取类型
收费全文 | 797篇 |
免费 | 46篇 |
国内免费 | 4篇 |
专业分类
耳鼻咽喉 | 5篇 |
儿科学 | 57篇 |
妇产科学 | 11篇 |
基础医学 | 99篇 |
口腔科学 | 10篇 |
临床医学 | 73篇 |
内科学 | 162篇 |
皮肤病学 | 27篇 |
神经病学 | 37篇 |
特种医学 | 20篇 |
外科学 | 93篇 |
综合类 | 57篇 |
预防医学 | 59篇 |
眼科学 | 5篇 |
药学 | 92篇 |
中国医学 | 9篇 |
肿瘤学 | 31篇 |
出版年
2023年 | 2篇 |
2022年 | 13篇 |
2021年 | 34篇 |
2020年 | 17篇 |
2019年 | 18篇 |
2018年 | 19篇 |
2017年 | 16篇 |
2016年 | 24篇 |
2015年 | 24篇 |
2014年 | 38篇 |
2013年 | 43篇 |
2012年 | 61篇 |
2011年 | 66篇 |
2010年 | 40篇 |
2009年 | 39篇 |
2008年 | 34篇 |
2007年 | 50篇 |
2006年 | 27篇 |
2005年 | 31篇 |
2004年 | 26篇 |
2003年 | 13篇 |
2002年 | 17篇 |
2001年 | 14篇 |
2000年 | 14篇 |
1999年 | 7篇 |
1998年 | 11篇 |
1997年 | 9篇 |
1996年 | 11篇 |
1994年 | 7篇 |
1993年 | 14篇 |
1992年 | 9篇 |
1991年 | 14篇 |
1990年 | 7篇 |
1989年 | 11篇 |
1988年 | 6篇 |
1987年 | 8篇 |
1986年 | 11篇 |
1985年 | 7篇 |
1984年 | 8篇 |
1983年 | 2篇 |
1982年 | 5篇 |
1981年 | 3篇 |
1977年 | 2篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1971年 | 2篇 |
1970年 | 1篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有847条查询结果,搜索用时 0 毫秒
21.
22.
Gaurav Bajpai Tulika Srivastava N. Patra Igamcha Moirangthem S. N. Jha D. Bhattacharyya Sk Riyajuddin Kaushik Ghosh Dharma R. Basaula Mahmud Khan Shun-Wei Liu Sajal Biring Somaditya Sen 《RSC advances》2018,8(43):24355
Substituting an ion of different size from that of the host element introduces lattice strain and defects. However, this mismatch may be significantly reduced by substituting an additional ion with a compensating size relative to the dopant. Such a double substitution might offer better solubility irrespective of the local distortions as well as the formation of defects in the valence states. Fe-substituted ZnO has been widely reported with conflicting results primarily arising from lack of chemical and structural homogeneity originating from preparation techniques, compositional fluctuations, and equivocal comprehension of actual solubility limits of the dopants. In this study, Ag ion has been incorporated in Fe-substituted ZnO to compensate the ionic size of Zn1−x[Fe0.8Ag0.2]xO (0 ≤ x ≤ 0.03125) by determining the solubility limit of the homogeneous material and their corresponding structural, mechanical, optical and magnetic properties have been investigated thoroughly. Co-substitution rearranges the lattice and leads to better crystal structures with tunable properties related to the amount of substitution.Substituting an ion of different size from that of the host element introduces lattice strain and defects. 相似文献
23.
Interactions between vascular endothelial cells and blood platelets have been investigated using a model microcirculation consisting of microcarrier beads colonized with human umbilical vein endothelial cells (HUVECs) and perfused with washed platelet suspensions. To simulate the effects of endothelial desquamation and exposure of subendothelium, fibrillar collagen in suspension was coinjected with the platelets. In this model, neither the passage of platelets alone nor collagen alone stimulated prostacyclin (PGI2) production by the HUVECs. Platelets activated by coinjection with collagen released thromboxane A2 (TXA2), and this was associated with the simultaneous production of PGI2 by the HUVECs. By means of double-isotope experiments with [3H]arachidonic acid (AA) incorporated into platelets and [14C]-AA into HUVECs, it was shown that all the PGI2 generated was derived from platelet AA and/or endoperoxides. This interpretation was strengthened by the finding that PGI2 production was not prevented by treatment of HUVECs with indomethacin followed by perfusion with collagen-stimulated platelets. AA metabolites in double-isotope label experiments were further characterized by reverse-phase chromatography, and it was shown that both cyclooxygenase and lipoxygenase products of the HUVECs were derived from platelet membrane lipid. Thrombin regularly produced transient PGI2 release, but showed rapid tachyphylaxis. Platelet-derived compounds including ADP, ATP, and platelet-activating factor (PAF) did not produce PGI2 release by HUVECs in this system. Thus, the transfer of AA and metabolites from collagen- stimulated platelets is likely to be the mechanism for PGI2 production in the context of minor degrees of endothelial desquamation. 相似文献
24.
25.
目的研究呼吸纯氧对健康人听感觉门控P50的影响。方法右利手、健康男性大学生志愿者28名,根据随机数字表分为对照组(n=12)和实验组(n=16)。佩戴面罩,对照组呼吸空气,实验组呼吸医用纯氧60 min。应用条件-测试刺激,记录吸氧前(pre0)、吸氧20 min(Oxy20)、吸氧50 min(Oxy50)、吸氧后30 min(post30)的脑电图,计算听觉P50潜伏期和P50感觉门控电位(S1与S2振幅之差)。结果 S1刺激在4个时间点各组P50潜伏期相对稳定(P0.7);吸氧50 min时,实验组比对照组P50潜伏期缩短(P0.05)。S2刺激在4个时间点各组P50潜伏期相对稳定(P0.30),两组间比较无显著性差异(P0.05)。对照组P50门控电位比较稳定(P=0.70),而实验组随吸氧时间逐渐延长,电位越来越高,停止吸氧后,电位迅速回落,Oxy20和post30(P=0.04)、Oxy50和post30(P=0.02)相比均有显著性差异。组间比较,4个时间点均无显著性差异(P0.05)。结论健康人吸60 min纯氧,可能缩短对刺激的反应时间,有增强听觉门控电位的趋势。 相似文献
26.
DC Bosanquet CN Jones N Gill P Jarvis MH Lewis 《Annals of the Royal College of Surgeons of England》2013,95(1):15-19
Introduction
Numerous strategies are employed routinely in an effort to lower rates of surgical site infections (SSIs). A laminar flow theatre environment is generally used during orthopaedic surgery to reduce rates of SSIs. Its role in vascular surgery, especially when arterial bypass grafts are used, is unknown.Methods
A retrospective review of a prospectively maintained database was undertaken for all vascular procedures performed by a single consultant over a one-year period. Cases were performed, via random allocation, in either a laminar or non-laminar flow theatre environment. Demographic data, operative data and evidence of postoperative SSIs were noted. A separate subgroup analysis was undertaken for patients requiring an arterial bypass graft. Univariate and multivariate logistical regression was undertaken to identify significant factors associated with SSIs.Results
Overall, 170 procedures were analysed. Presence of a groin incision, insertion of an arterial graft and a non-laminar flow theatre were shown to be predictive of SSIs in this cohort. In the subgroup receiving arterial grafts, only a non-laminar flow theatre environment was shown to be predictive of an SSI.Conclusions
This study suggests that laminar flow may reduce incidences of SSI, especially in the subgroup of patients receiving arterial grafts. 相似文献27.
28.
29.
Hanna Wootz Travis M. Rotterman Anders Enjin Kalicharan Patra Elodie André Brigitte Van Zundert Klas Kullander Francisco J. Alvarez 《The Journal of comparative neurology》2013,521(7):1449-1469
Motor neurons become hyperexcitable during progression of amyotrophic lateral sclerosis (ALS). This abnormal firing behavior has been explained by changes in their membrane properties, but more recently it has been suggested that changes in premotor circuits may also contribute to this abnormal activity. The specific circuits that may be altered during development of ALS have not been investigated. Here we examined the Renshaw cell recurrent circuit that exerts inhibitory feedback control on motor neuron firing. Using two markers for Renshaw cells (calbindin and cholinergic nicotinic receptor subunit alpha2 [Chrna2]), two general markers for motor neurons (NeuN and vesicular acethylcholine transporter [VAChT]), and two markers for fast motor neurons (Chondrolectin and calcitonin‐related polypeptide alpha [Calca]), we analyzed the survival and connectivity of these cells during disease progression in the Sod1G93A mouse model. Most calbindin‐immunoreactive (IR) Renshaw cells survive to end stage but downregulate postsynaptic Chrna2 in presymptomatic animals. In motor neurons, some markers are downregulated early (NeuN, VAChT, Chondrolectin) and others at end stage (Calca). Early downregulation of presynaptic VAChT and Chrna2 was correlated with disconnection from Renshaw cells as well as major structural abnormalities of motor axon synapses inside the spinal cord. Renshaw cell synapses on motor neurons underwent more complex changes, including transitional sprouting preferentially over remaining NeuN‐IR motor neurons. We conclude that the loss of presynaptic motor axon input on Renshaw cells occurs at early stages of ALS and disconnects the recurrent inhibitory circuit, presumably resulting in diminished control of motor neuron firing. J. Comp. Neurol. 521:1449–1469, 2013. © 2012 Wiley Periodicals, Inc. 相似文献
30.
Currently, considerable efforts have been devoted to the detection and quantification of hazardous multi-analytes using a single probe. Herein, we have developed a simple, environment-friendly colourimetric sensor for the sensitive, selective and rapid detection of Ni2+ and Cu2+ ions using a simple organic Schiff base ligand L in methanol–Tris–HCl buffer (1 : 1 v/v, 10 mM, pH = 7.2). The probe L exhibited a binding-induced colour change from colourless to yellow and fluorescence quenching in the presence of both Ni2+ and Cu2+ ions. The interactions between L and the respective metal ions were studied by Job''s plot, electrospray ionisation-mass spectrometry (ESI-MS), Fourier-transform infrared spectroscopy (FT-IR), density functional theory (DFT) and time-dependent density functional theory (TDDFT) calculations. The limit of detection (LOD) of L towards Ni2+ and Cu2+ was calculated to be 7.4 × 10−7 M and 4.9 × 10−7 M, respectively. Furthermore, the L–Cu2+ complex could be used as a new cascade fluorescent-colourimetric sensor to detect CN− ions with a very low level of detection (40 nM). Additionally, L could operate in a wide pH range, and thus was successfully applied for the detection and quantification of Ni2+ and Cu2+ in environmental samples, and for building OR- and IMPLICATION-type logic gates.A novel colorimetric chemosensor L has been developed for the detection of Ni2+ and Cu2+ ions. The obtained L–Cu2+ complex can be used as a new cascade fluorescent-colorimetric sensor for the nanomolar detection of CN− ions. This chemosensor has practical application. 相似文献