全文获取类型
收费全文 | 8587篇 |
免费 | 627篇 |
国内免费 | 30篇 |
专业分类
耳鼻咽喉 | 105篇 |
儿科学 | 217篇 |
妇产科学 | 237篇 |
基础医学 | 1281篇 |
口腔科学 | 155篇 |
临床医学 | 1036篇 |
内科学 | 1736篇 |
皮肤病学 | 199篇 |
神经病学 | 976篇 |
特种医学 | 265篇 |
外科学 | 844篇 |
综合类 | 68篇 |
一般理论 | 1篇 |
预防医学 | 760篇 |
眼科学 | 118篇 |
药学 | 562篇 |
中国医学 | 41篇 |
肿瘤学 | 643篇 |
出版年
2024年 | 9篇 |
2023年 | 93篇 |
2022年 | 172篇 |
2021年 | 378篇 |
2020年 | 251篇 |
2019年 | 263篇 |
2018年 | 304篇 |
2017年 | 224篇 |
2016年 | 282篇 |
2015年 | 326篇 |
2014年 | 369篇 |
2013年 | 506篇 |
2012年 | 739篇 |
2011年 | 750篇 |
2010年 | 424篇 |
2009年 | 374篇 |
2008年 | 585篇 |
2007年 | 512篇 |
2006年 | 497篇 |
2005年 | 483篇 |
2004年 | 406篇 |
2003年 | 347篇 |
2002年 | 343篇 |
2001年 | 45篇 |
2000年 | 33篇 |
1999年 | 41篇 |
1998年 | 69篇 |
1997年 | 42篇 |
1996年 | 35篇 |
1995年 | 32篇 |
1994年 | 33篇 |
1993年 | 21篇 |
1992年 | 15篇 |
1991年 | 13篇 |
1990年 | 14篇 |
1989年 | 14篇 |
1988年 | 18篇 |
1987年 | 9篇 |
1986年 | 7篇 |
1985年 | 8篇 |
1984年 | 15篇 |
1983年 | 12篇 |
1982年 | 19篇 |
1981年 | 6篇 |
1980年 | 9篇 |
1978年 | 11篇 |
1977年 | 6篇 |
1975年 | 10篇 |
1937年 | 5篇 |
1928年 | 5篇 |
排序方式: 共有9244条查询结果,搜索用时 156 毫秒
61.
Immunoglobulin molecules on the surface of mouse thymus cells have been shown by immunofluorescence. Ninety-five to 100 per cent of thymus lymphocytes were found to bind polyvalent rabbit anti-mouse immunoglobulin, although the density of the fluorescence was much less than that on the surface of B lymphocytes derived from the spleen.
Two purified antisera, an anti-IgM and an anti-kappa chain serum, also stained the cells.
The resynthesis of these immunoglobulin molecules in vitro was demonstrated after they had been removed with pronase.
相似文献62.
63.
The 505 amino acid L1 protein of the human papillomavirus type 11 (HPV 11) is the major capsid polypeptide that has been shown to self-assemble into virus-like particles (VLPs) in vivo and in vitro. While L1 is essential for viral infection, expression studies in mammalian cells have been hampered by different codon preference between the virus and its host. To optimize L1 gene expression in mammalian cells, we converted wild-type HPV 11 L1 (11 L1wt) codons to those more common in human genes. The modified HPV 11 L1 gene (11 L1h) generated protein levels that were at least 100-fold higher than those of wild-type HPV 11 L1, while no obvious differences were seen in the level of mRNA. HPV 11 L1 protein was detected in mammalian epithelial and fibroblast cells, by immunoblotting and indirect immunofluorescence (IIF) techniques. Unlike the situation in situ, IIF revealed the presence of L1 mainly at perinuclear sites. Virus-like particles assembled intranuclearly only to a low extent, as indicated by transmission electron microscopy. DNA vaccination using the HPV 11 L1h gene yielded a drastic increase in L1-specific antibody production in mice as compared to immunization with the wild-type gene. 相似文献
64.
Riitta Hari Ritva Hänninen Timo Mäkinen Veikko Jousmäki Nina Forss Mika Seppä Oili Salonen 《Neuroscience letters》1998,240(3):998-134
We describe patient E.P. who occasionally perceives a ‘ghost' hand which copies the previous positions of the left hand with a 0.5–1 min time lag, but follows the movement patterns of the right hand. The symptoms started after an operation of a ruptured aneurysm, followed by an infarction of the right frontal lobe; E.P. also has a previously lesioned corpus callosum. Neuromagnetic recordings revealed that activity of the left secondary somatosensory cortex was strongly suppressed during the ghost arm percept, thereby providing an objective correlate for E.P.'s sensations. We conclude that simultaneous mental contents about body scheme may be based on neural information extracted at considerably different times, resulting in fragmentation of bodily awareness. 相似文献
65.
Individual specific antigenic rubella virus (RV) structural proteins are required for accurate serological diagnosis of acute and congenital rubella infections as well as rubella immune status. The RV envelope glycoprotein E1 is the major target antigen and plays an important role in viral-specific immune responses. The native virion is difficult to produce in large quantities and the protein subunits are also difficult to isolate without loss of antigenicity. The production of a soluble RV E1 (designated E1ΔTm) using the baculovirus-insect cell expression system is described. In contrast to wild-type RV E1, the genetically engineered E1ΔTm protein lacks a transmembrane anchor. It behaved as a secretory protein and was secreted abundantly from insect cells. Pulse-chase studies were used to examine the synthesis, glycosylation, and secretion of E1ΔTm by the insect cells. The secreted E1ΔTm protein was purified from serum-free medium by onestep immunochromatography. The purified E1ΔTm protein retained full antigenicity and may be a convenient source of E1 protein for use in diagnostic assay and rubella vaccine development. 相似文献
66.
Tatyana B. Nesterova Sarah M. Duthie Nina A. Mazurok Al'bina A. Isaenko Nadezhda V. Rubtsova Suren M. Zakian Neil Brockdorff 《Chromosome research》1998,6(1):41-48
Comparative mapping of X-linked genes has progressed rapidly since Ohno's prediction that genes on the X chromosome should be conserved as a syntenic group in all mammals. Although several conserved blocks of homology between human and mouse have been discovered, rearrangements within the X chromosome have also been characterized. More recently, some exceptions to Ohno's law have been reported. We have used fluorescence in situ hybridization (FISH) to map five genes, Gla, G6pd, Hprt, Pgk1 and Xist, to two of the largest conserved segments of X material in five members of the genus Microtus (grey vole) and show that vole X chromosomes demonstrate greater homology to human than to mouse. Cytogenetic analysis indicates a relatively high frequency of rearrangement during vole evolution, although certain blocks of homology appear to be highly conserved in all species studied to date. On this basis we were able to predict the probable location of the rat X inactivation centre (Xic) based solely on high-resolution G-banding. Our prediction was then confirmed by mapping the rat Xist gene by FISH. The possible significance of conserving long-range chromosome structure in the vicinity of the Xic is discussed with respect to the mechanism of X inactivation. 相似文献
67.
A cross-sectional investigation of psychosocial variables in 63 female employees matched for experienced pain was conducted to study the difference between back pain sufferers who were working (Copers) and those who were off work (Dysfunctional). The subjects reported moderate to severe pain often or always during the past year and were employed at the same hospital. Thirty-seven women who had not been off work for pain made up the Copers group, whereas 26 women who had been off work for their pain made up the Dysfunctional group. Subjects were interviewed and completed a battery of questionnaires designed to penetrate level of dysfunction, perceived health, work and social satisfaction, perceived workload, coping strategies, and pain beliefs. Multiple covariate analyses that controlled for perceived workload, smoking, low-back mobility, and obesity revealed significant differences between the groups on levels of functioning, pain beliefs, and coping strategies used. Dysfunctional subjects had stronger beliefs that pain was directly related to activities that they had little control over their pain, that their health was poor, and that they tended to focus more on their pain. A discriminant analysis correctly classified 83% of the subjects as to work status based on six psychosocial variables. These results not only demonstrate the importance of psychosocial factors in back pain, but underscore the fact that work absence for back pain may he controlled by psychological factors related to beliefs and coping strategies. Future research may attempt to use these factors in the screening of patients. 相似文献
68.
Evaluation of dry and wet transported intravaginal swabs in detection of Chlamydia trachomatis and Neisseria gonorrhoeae infections in female soldiers by PCR 总被引:3,自引:0,他引:3
下载免费PDF全文
![点击此处可从《Journal of clinical microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Gaydos CA Crotchfelt KA Shah N Tennant M Quinn TC Gaydos JC McKee KT Rompalo AM 《Journal of clinical microbiology》2002,40(3):758-761
Screening women for sexually transmitted diseases (STD) in nonclinic settings is highly desirable because many infections are asymptomatic. This is especially true for military women, for whom logistical, social, and other job-related obstacles present barriers to accessing medical care. We assessed the accuracy of intravaginal swabs transported by mail in a wet versus a dry state for PCR (Amplicor CT/NG test) detection of chlamydia and gonorrhea infections in a cross-sectional study of 793 active-duty military women attending an STD clinic. PCR tests of vaginal swabs (wet and dry) were compared to local clinical methods used on cervical swabs. Standard wet vaginal swab PCR testing detected more chlamydia (11.6%) than cervical enzyme immunoassay (9.3%). For detection of chlamydia using wet swabs, the sensitivity and specificity compared with adjudicated true positives were 94.6% (87 of 92) and 99.3% (696 of 701), respectively. Comparing dry swabs to true-positives for chlamydia, the sensitivity was 91.3% (84 of 92) and the specificity was 99.3% (696 of 701). Standard wet vaginal swab PCR detected more gonorrhea (3.3%) than routine cervical culture (2.1%). The sensitivity and specificity of PCR testing of wet swabs compared to true-positives (infected patients) were 96.3% (26 of 27) and 98.2% (752 of 766) for gonorrhea, respectively. For gonorrhea, the sensitivity and specificity of dry swabs compared to true-positives (infected patients) were 88.9% (24 of 27) and 98.3% (753 of 766), respectively. PCR testing of wet and dry transported intravaginal swabs to detect chlamydia and gonorrhea infections was an accurate diagnostic method for military women. 相似文献
69.
70.
This study investigated how the human auditory brainstem represents constituent elements of speech sounds differently in children with language-based learning problems (LP, n = 9) compared to normal children (NL, n = 11), especially under stress of rapid stimulation. Children were chosen for this study based on performance on measures of reading and spelling and measures of syllable discrimination. In response to the onset of the speech sound /da/, wave V-V(n) of the auditory brainstem response (ABR) had a significantly shallower slope in LP children, suggesting longer duration and/or smaller amplitude. The amplitude of the frequency following response (FFR) was diminished in LP subjects over the 229-686 Hz range, which corresponds to the first formant of the/da/ stimulus, while activity at 114 Hz, representing the fundamental frequency of /da/, was no different between groups. Normal indicators of auditory peripheral integrity suggest a central, neural origin of these differences. These data suggest that poor representation of crucial components of speech sounds could contribute to difficulties with higher-level language processes. 相似文献