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101.
Bauer E Gendo A Madl C Garo F Roth E Kramer L 《The International journal of artificial organs》2002,25(10):923-928
In cirrhotic patients, plasma amino acid levels are severely deranged. A decreased ratio of branched-chain to aromatic amino acids (Fischer ratio) has been implicated in the pathogenesis of hepatic encephalopathy. In this prospective study, we investigated the effects of extracorporeal detoxification on amino acid levels using a sorbent suspension dialysis system. Twenty patients with documented cirrhosis and hepatic encephalopathy grade II-III not responding to standard treatment were randomized to receive either six hours of sorbent dialysis and standardized conventional medical treatment or ongoing medical treatment alone. In contrast to previous uncontrolled studies, no significant effect on amino acid levels, Fischer ratio or clinical grade of hepatic encephalopathy was detected in either treatment group. In conclusion, a 6-hour treatment with sorbent dialysis did not significantly influence plasma levels of amino acids and did not ameliorate the clinical grade of hepatic encephalopathy. 相似文献
102.
Summary 100 stool extracts from patients with poliomyelitis or from individuals after oral poliomyelitis vaccination were inoculated simultaneously into HeLa, monkey kidney, and human thyroid cultures. In most cases (88 times) a cytopathic effect was seen in all three cultures. Failures were most frequently encountered in thyroid, rarely in monkey kidney cells. They were due either to thyroid culture lots with reduced viral susceptibility or to small amounts of virus in the stool specimens. No qualitative difference was found in the host range of individual virus strains. With attenuated viruses the cytopathic effect in HeLa cells as a rule was delayed for several days.
Die Untersuchungen wurden mit Unterstützung der Deutschen Vereinigung zur Bekmpfung der Kinderlähmung e.V. durchgeführt.
Wir danken Herrn Privatdoz. Dr. F.Müller, Düsseldorf, für die freundliche überlassung einiger virushaltiger Stuhlproben. Herr Dr. A. R.Ababio, Homburg, war uns bei der Beschaffung von Stuhlproben nach oraler Poliomyelitisimpfung behilflich. Wir danken schließlich Fräulein E.Baschleben, Frau H.Gelderblom, Frau H.Kaiser und Fräulein I.Lamy für ihre Hilfe bei den Untersuchungen. 相似文献
Die Untersuchungen wurden mit Unterstützung der Deutschen Vereinigung zur Bekmpfung der Kinderlähmung e.V. durchgeführt.
Wir danken Herrn Privatdoz. Dr. F.Müller, Düsseldorf, für die freundliche überlassung einiger virushaltiger Stuhlproben. Herr Dr. A. R.Ababio, Homburg, war uns bei der Beschaffung von Stuhlproben nach oraler Poliomyelitisimpfung behilflich. Wir danken schließlich Fräulein E.Baschleben, Frau H.Gelderblom, Frau H.Kaiser und Fräulein I.Lamy für ihre Hilfe bei den Untersuchungen. 相似文献
103.
Takayuki Kadohama Nobuyuki Akasaka Kengo Nishimura Yuji Hoshino Tadahiro Sasajima Bauer E Sumpio 《Endothelium》2006,13(1):43-50
Fluid shear stress is thought to be important in maintaining the phenotype of endothelial cells (ECs) in vivo. The purpose of the study was to determine the effect of varying levels of laminar shear stress on EC elongation and alignment and the role of p38 mitogen-activated protein kinase (MAPK) on the morphologic change induced by shear stress. Cultured bovine aortic ECs were subjected to 1, 4, 7, 14, or 20 dyne/cm(2) laminar steady shear stress. On morphometric analysis of static ECs, the average orientation angle was 41 degrees , whereas after 24 h shear stress at 1, 4, 7, 14, and 20 dyne/cm(2) the angles were 34 degrees, 33 degrees, 16 degrees, 11 degrees, and 10 degrees, respectively. The shape index of static ECs was 0.76, whereas the indexes of ECs exposed to shear stress were 0.72, 0.72, 0.65, 0.50, and 0.47, respectively. The time and the magnitude of activation of p38 MAPK were dependent on the level of shear stress. The results indicate that a minimum shear stress of 7 to 14 dynes/cm(2) is necessary for cell alignment and elongation and this correlates with activity of p38 MAPK. ECs exposed to shear stress in the presence of the p38 MAPK inhibitor SB-203580 did not orient in any manner and the shape index was similar to the static cells. 相似文献
104.
H. W. Bauer 《Journal of molecular medicine (Berlin, Germany)》1981,59(4):149-155
Summary Long-term studies of 2-PAG in sera of patients with breast cancer, bronchial carcinoma, gynaecological carcinoma, melanoma and laryngeal carcinoma have proved that 2-PAG is a possible laboratory parameter for the assessment of recurrence of tumour and metastasis. The results published for trophoblastic tumour and gastrointestinal carcinoma are still divergent. Because of the large individual range of 2-PAG concentrations and the widely differing 2-PAG levels in men and women single determinations of this protein are without value. A detailed classification of the pathological mechanisms to which these proteins are submitted is still missing and consequently we have no fundamental knowledge of diseases that apart from pregnancy and neoplasia lead to changes in the physiological 2-PAG serum concentration.Dedicated to Prof. Dr. E. Schmiedt to his 60th birthday (20th of November 1980) 相似文献
105.
The distributions of occludin and claudin-1, two tight junction–associated integral membrane proteins were investigated by immunohistochemical analysis of whole-mount preparations of the blood vessels in the myelinated streak of the rabbit retina. Light microscopy revealed that occludin and claudin-1 immunoreactivities were abundant along the interface of adjacent endothelial cells of all blood vessels. Electron microscopy revealed that both proteins were distributed in a regular pattern (at regular intervals of approximately 80 nm) along the length of tight junctions, probably in the regions of tight junction strands. No other structures or cell types expressed either of these two proteins in the myelinated streak. Whereas occludin immunoreactivity was concentrated only at the tight junction interface, claudin-1 immunoreactivity also extended into the cytoplasm of the endothelial cells, suggesting a different structural role for claudin-1 than for occludin at tight junctions. Retinal pigment epithelial cells expressed occludin around their entire circumference, consistent with the function of these cells as a barrier separating the retina from the leaky vessels of the choroid. Also consistent with the association of occludin expression with vessels that exhibit functional tight junctions, this protein was expressed at only a low level in, and showed an irregular distribution along, the vessels of the choroid, a vascular bed that lacks blood-barrier properties. Further, the distribution of occludin was examined during formation and remodelling of the rat retinal vasculature. Occludin expression was evident at the leading edge of vessel formation and was found on all vessels in both the inner and outer vascular plexus. Numerous vascular segments at the early stage of vascular formation and regression lost occludin expression. The biological significance of this transient loss of occludin expression in terms of barrier function remains to be elucidated. 相似文献
106.
Shimansky Y Wang JJ Bauer RA Bracha V Bloedel JR 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》2004,155(2):156-172
Although the cerebellum has been shown to be critical for the acquisition and retention of adaptive modifications in certain reflex behaviors, this structures role in the learning of motor skills required to execute complex voluntary goal-directed movements still is unclear. This study explores this issue by analyzing the effects of inactivating the interposed and dentate cerebellar nuclei on the adaptation required to compensate for an external elastic load applied during a reaching movement. We show that cats with these nuclei inactivated can adapt to predictable perturbations of the forelimb during a goal-directed reach by including a compensatory component in the motor plan prior to movement initiation. In contrast, when comparable compensatory modifications must be triggered on-line because the perturbations are applied in randomized trials (i.e., unpredictably), such adaptive responses cannot be executed or reacquired after the interposed and dentate nuclei are inactivated. These findings provide the first demonstration of the condition-dependent nature of the cerebellums contribution to the learning of a specific volitional task. 相似文献
107.
Comparison of the real-time PCR method and the Gen-Probe amplified Mycobacterium tuberculosis direct test for detection of Mycobacterium tuberculosis in pulmonary and nonpulmonary specimens 下载免费PDF全文
Lemaître N Armand S Vachée A Capilliez O Dumoulin C Courcol RJ 《Journal of clinical microbiology》2004,42(9):4307-4309
Real-time PCR was compared to Amplified Mycobacterium tuberculosis Direct Test (AMTDII) for 100 clinical specimens. The overall sensitivities of the real-time PCR method and AMTDII were similar for respiratory and nonrespiratory specimens. However, real-time PCR seemed to be less susceptible to amplification inhibitors than AMTDII. 相似文献
108.
Transgenic rat model of Huntington's disease 总被引:12,自引:0,他引:12
von Hörsten S Schmitt I Nguyen HP Holzmann C Schmidt T Walther T Bader M Pabst R Kobbe P Krotova J Stiller D Kask A Vaarmann A Rathke-Hartlieb S Schulz JB Grasshoff U Bauer I Vieira-Saecker AM Paul M Jones L Lindenberg KS Landwehrmeyer B Bauer A Li XJ Riess O 《Human molecular genetics》2003,12(6):617-624
Huntington's disease (HD) is a late manifesting neurodegenerative disorder in humans caused by an expansion of a CAG trinucleotide repeat of more than 39 units in a gene of unknown function. Several mouse models have been reported which show rapid progression of a phenotype leading to death within 3-5 months (transgenic models) resembling the rare juvenile course of HD (Westphal variant) or which do not present with any symptoms (knock-in mice). Owing to the small size of the brain, mice are not suitable for repetitive in vivo imaging studies. Also, rapid progression of the disease in the transgenic models limits their usefulness for neurotransplantation. We therefore generated a rat model transgenic of HD, which carries a truncated huntingtin cDNA fragment with 51 CAG repeats under control of the native rat huntingtin promoter. This is the first transgenic rat model of a neurodegenerative disorder of the brain. These rats exhibit adult-onset neurological phenotypes with reduced anxiety, cognitive impairments, and slowly progressive motor dysfunction as well as typical histopathological alterations in the form of neuronal nuclear inclusions in the brain. As in HD patients, in vivo imaging demonstrates striatal shrinkage in magnetic resonance images and a reduced brain glucose metabolism in high-resolution fluor-deoxy-glucose positron emission tomography studies. This model allows longitudinal in vivo imaging studies and is therefore ideally suited for the evaluation of novel therapeutic approaches such as neurotransplantation. 相似文献
109.
Heinz Bauer 《Archives of virology》1965,15(2):253-256
Summary The hemagglutination of human and rat red blood cells by adeno-virus type 27 is completely inhibited by treatment of the virus with p-chlormercuribenzoic acid (PCMB), N-ethylmaleimid (NEM) and KJO4. Treatment of the erythrocytes has no effect. The action of PCMB and NEM is fully reverted by reduced glutathione or 1-cysteine. Under the same conditions, the hemagglutinins of other adenovirus types belonging also toRosen's group II are unaffected by these reagents.
Mit Unterstützung der Deutschen Forschungsgemeinschaft. 相似文献
Mit Unterstützung der Deutschen Forschungsgemeinschaft. 相似文献
110.
Oncogenicity of non-transforming mutants of avian sarcoma viruses 总被引:11,自引:0,他引:11