Lead in the mother-newborn system as an indicator of chemical load hazard in unfavorable environmental areas

The paper deals with the studies of the levels of lead in the biological media in women in labour and their neonatal infants who live in poor environmental areas. A comparison was made of the levels of lead in the biological media of the women in labour and neonates from the experimental and control groups. There was a relationship between the levels of lead in the biological media of the newborn and the mother. A total of 7 different biosubstrates from the women in labour and neonatal babies were examined. The studies were made by atomic absorption spectrophotometry on a Perkin Elmer 3110 device.     

Rapid progression of passaged mouse hepatocarcinoma associated with the loss of cell polarity

Two transplantable differentiated mouse hepatocarcinomas were obtained in B6 D2 F1 mice from primary tumors induced by initiation (NDEA)-promotion (phenobarbital) protocol. Both HC were slowly growing with time from passage to passage of 5-7 months (s-HC). A fast-growing variant with time between passages of 2-3 weeks appeared in one mouse on the third passage (f-HC). The three HC strains constantly retained their phenotype. The s-HCs were characterized by prominent cell polarity according to organization of the cytoskeleton and distribution of domain-specific membrane-associated markers. Cell polarity was completely destroyed in the fast-growing variant. Cell adhesion in the latter tumor was very low. An in vitro growing strain of f-HC was easily obtained enabling experimental study of regulation of progression in HCs.     

The rehabilitation of patients with diabetes mellitus and ischemic heart disease

The results of bicycle ergometry testing were studied in 20 patients with diabetes mellitus of type II without clinical signs of circulatory insufficiency, 21 patients with postinfarction cardiosclerosis with undisturbed carbohydrate tolerance, and in 18 healthy persons. The blood level of glucose in patients with diabetes mellitus was investigated on an empty stomach and 30, 60 and 120 min. after physical exercising. Similar changes of bicycle ergometry results were found in diabetic and CHD patients. In compensation of diabetes in response to graded physical exercise glycemic changes were absent, in decompensation glycemia increased in 30 and 60 min. and returned to the basal level 120 min. after testing. Since the results of bicycle exercises in diabetic (compensated) and CHD patients were the same, the principles of their physical rehabilitation must be identical. In patients with decompensated diabetes mellitus a hyperglycemic reaction to graded physical exercise serves the basis for reduction of motor activity in patients with decompensated diabetes mellitus.     

Application of vacuum drainage of purulent wound in the treatment of phlegmon of maxillofacial region with predominance of putrefactive-necrotic lesions

There was established high efficacy of method of the low dosed vacuum draining of wound with predominance of putrefactive-necrotic affection was established in patients with phlegmon of maxillofacial region.     

Human mini-antibodies to orthopoxviruses

A library of human scFv antibodies displayed on the surface of bacteriophages (MRC, Cambridge, England) was panned against the Elstree strain of vaccinia virus (VACV), which resulted in the phage repertoire enriched with clones positive to the strain. Individual clones from the repertoire were screened for binding, independently, to the vaccinia and ectromelia viruses; phage antibodies to the orthopoxviruses were selected. Ten unique antibodies were identified after their Vh- and Vl-genes were sequenced. All selected antibodies were assayed by ELISA for binding to the vaccinia, cowpox and ectromelia viruses. Furthermore, all selected antibodies were assayed for binding with major alastrim strains of the live variola virus. According to the results, the above phage antibodies recognized genus-specific epitopes, some of which differed in their conformation.