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31.
Hayashi K Katano Y Takeda Y Honda T Ishigami M Itoh A Hirooka Y Nakano I Yano M Goto H Yoshioka K Toyoda H Kumada T 《Journal of medical virology》2007,79(4):366-373
Hepatitis B virus (HBV) has been classified into eight genotypes and can be further divided into several subgenotypes that have different geographic distributions. Because of increased human migration, the prevalence of rare subgenotypes is increasing in Japanese patients with acute hepatitis B. Lamivudine-resistant strains of HBV have begun to emerge in association with chronic hepatitis B. The aim of this study was to investigate the distribution of HBV subgenotypes and lamivudine-resistant strains in patients in Japan with acute hepatitis B. One hundred twenty-three patients with acute hepatitis B and 123 with chronic hepatitis B were studied. HBV subgenotypes and lamivudine-resistance mutations were determined by direct sequencing of the preS and polymerase region, respectively. HBV subgenotypes Aa (n=3), Ae (n=23), Ba (n=7), Bj (n=3), Cs (n=7), Ce (n=76), D (n=2), and H (n=2) were detected in patients with acute hepatitis. In patients with chronic hepatitis, HBV subgenotypes Ae (n=4), Ba (n=1), Bj (n=18), and Ce (n=100) were found. Non-common Japanese subgenotypes, that is, non-Bj and non-Ce, were detected more frequently in patients with acute hepatitis (35.8%) than in patients with chronic hepatitis (4.1%) (Odds ratio, 0.076; 95%CI, 0.029-0.200; P<0.0001). Lamivudine-resistance mutations were detected in chronic hepatitis patients with breakthrough hepatitis but not in other patients. In conclusion, the prevalence of uncommon Japanese HBV subgenotypes is expected to increase, although lamivudine-resistant strains have not yet been found in patients with acute hepatitis B. 相似文献
32.
33.
Carcinomatous Lymphatic Invasion in Early Gastric Cancer Invading Into the Submucosa 总被引:5,自引:0,他引:5
Sumiya Ishigami MD Shoji Natsugoe MD Shuuichi Hokita MD Masahiro Tokushige MD Tetsushi Saihara MD Teruhiko Watanabe MD Kuniaki Aridome MD Hirohumi Iwashige MD Takashi Aikou MD 《Annals of surgical oncology》1999,6(3):286-289
Background: Lymphatic invasion is a risk factor for lymph node metastases in patients with gastric cancer. No studies have been reported, however, on the correlation between lymphatic invasion and lymph node metastasis in early gastric cancer invading into the submucosa.Methods: We performed a retrospective analysis of lymphatic invasion in 170 patients with early gastric cancer invading into the submucosa.Results: Lymphatic invasion was found in 76 patients. Lymphatic invasion correlated significantly with the presence of lymph node metastasis and vascular invasion (P < .05) and with the degree of cancerous submucosal involvement (P < .05). The presence of lymph node metastasis also correlated with the grade of submucosal invasion and lymphatic invasion. The 5-year survival of patients with lymphatic invasion was poorer than that of patients without lymphatic invasion (P < .05). Node-negative patients had similar survival, regardless of the presence of lymphatic invasion. All patients with severe lymphatic invasion had sm3 invasion and lymph node metastases.Conclusion: Although lymphatic invasion is the first stage of lymph node metastasis, lymphatic invasion in itself does not have clinical importance except for severe invasion in early gastric cancer. It is possible to predict lymph node metastases from the combined evaluation of degree of lymphatic invasion and submucosal involvement of the tumor in patients with early gastric cancer invading into the submucosa. 相似文献
34.
Sumiya Ishigami M.D. Shoji Natsugoe M.D. Yoshikazu Uenosono M.D. Yoichi Hata M.D. Aikihiro Nakajo M.D. Futoshi Miyazono M.D. Masataka Matsumoto M.D. Shuichi Hokita M.D. Takashi Aikou M.D. 《Journal of gastrointestinal surgery》2003,7(6):735-739
The sentinel node (SN) is regarded as the first drainage lymph node, and tumor cells are considered likely to directly affect
the SN. However, few reports have identified differences between SNs and non-SNs in cancer patients. Subjects in this study
included 27 patients with gastric cancer who underwent curative operation and intraoperative detection of SNs by radioisotope
methods. The mean number of SNs was 3.2 (range 1 to 5). Degree of infiltration of natural killer cells, dendritic cells, MIB-1
labeling index, and CD3-ξ expression of lymphocytes in SNs and non-SNs were examined by means of immunohistochemical methods.
Degree of infiltration was compared according to depth of invasion and between SNs and non-SNs. Patients with early-stage
cancer displayed a greater degree of infiltration of MIB-1 labeling index and CD3-ξ expression than patients with pT2 or pT3
lesions (P<0.05). The MIB-1 labeling index in SNs was significantly lower than that in non-SNs (P<0.05). However, no significant difference was observed in infiltration of natural killer cells, dendritic cells, or CD3-ξ.
Morphologic changes of dendritic cells in SNs were not definite. Our results suggest that SNs in gastric cancer might not
be suppressed, unlike in breast cancer and melanoma. SN paralysis may depend on tumor- and organ-specific characteristics
or exogenous stimulation from the gastric mucosa. Studies in progress will help to identify immunologic paralysis of the SN
in various types of cancer. Attention must therefore be paid to organ specificity. 相似文献
35.
Hideyuki Ogawa Toshiyuki Ishikawa Kouhei Matsushita Katsumi Matsumoto Tomoaki Ishigami Teruyasu Sugano Kazuaki Uchino Satoshi Umemura Shinichi Sumita Kazuo Kimura Takeshi Nakagawa Makoto Shimizu Hideo Nishikawa Atsunobu Kasai Yukio Kioka 《Circulation journal》2008,72(5):700-704
BACKGROUND: Several preliminary studies have indicated that atrial pacing can prevent atrial tachyarrhythmias. The suggested mechanisms by which pacing may be effective include suppression of premature atrial beats. METHODS AND RESULTS: The Atrial Pacing Preference (APP; Guidant, St Paul, MN, USA) algorithm allows the pacemaker to maintain a pacing rate slightly higher than the sinus rate. The preventive effects of APP on paroxysmal atrial fibrillation (AF) were studied in 51 patients (70+/-11 years). Nine patients did not complete the protocol. The pacemaker was programmed in random order to APP off and APP on at 3 different settings (ie, 8, 16 and 32 cycles) for 4 weeks each, using a cross-over design. Percentage atrial pacing was lower in APP off than at the other settings. Premature beat counts were greater in APP off than at the other settings. There was a significant difference in mode switch episode counts between APP off and the most effective setting (3,818+/-15,356 vs 596+/-1,719; p<0.01). CONCLUSIONS: The APP algorithm is a promising method for preventing atrial tachyarrhythmia in patients with an implanted pacemaker and AF. Optimizing the setting of the APP algorithm is an important issue in the prevention of AF. 相似文献
36.
Rapid reduction in BCR‐ABL1 transcript predicts deep molecular response in dasatinib‐treated chronic‐phase chronic myeloid leukaemia patients 下载免费PDF全文
Kazunori Murai Kohei Yamaguchi Shigeki Ito Takuto Miyagishima Motohiro Shindo Kentaro Wakasa Mitsue Inomata Takahiro Nagashima Takeshi Kondo Nozomu Fujimoto Satoshi Yamamoto Masakatsu Yonezumi Tatsuo Oyake Shugo Kowata Yasuhiko Tsukushi Takahiro Mine Kuniaki Meguro Kazuhiko Ikeda Reiko Watanabe Souichi Saito Shinji Sato Katsushi Tajima Takaaki Chou Kohmei Kubo Koji Oba Junichi Sakamoto Yoji Ishida The Inter‐Michinoku Dasatinib Study Group 《European journal of haematology》2018,100(1):27-35
37.
Hata K Watanabe T Kawamura YJ Ishigami H Kanazawa T Tada T Zhao B Koketsu S Nagawa H 《Digestive diseases and sciences》2003,48(12):2310-2314
38.
Comparison of direct sequencing and Invader assay for Y93H mutation and response to interferon‐free therapy in hepatitis C virus genotype 1b 下载免费PDF全文
Kazuhiko Hayashi Masatoshi Ishigami Yoji Ishizu Teiji Kuzuya Takashi Honda Hiroki Kawashima Tetsuya Ishikawa Yoshihiko Tachi Masashi Hattori Yoshiaki Katano Hidemi Goto Yoshiki Hirooka 《Journal of gastroenterology and hepatology》2018,33(1):249-255
Background and Aim
Virologic failure of interferon‐free therapy has been associated with Y93H mutation in the non‐structure 5A region in hepatitis C virus (HCV) genotype 1b, and screening is recommended. A simple assay based on Q‐Invader technology was developed for Y93H mutant screening to reduce cost and effort. The present study sought to compare two methods of detection of Y93H mutation and to evaluate the effect of Y93H mutation on response to interferon‐free therapy.Methods
Y93H mutation was examined in 258 patients with HCV genotype 1b using both direct sequencing analysis and the polymerase chain reaction (PCR)‐Invader assay. Daclatasvir and asunaprevir or ledipasvir and sofosbuvir therapy was administered to 205 patients whose sustained virological responses (SVR) were checked.Results
Hepatitis C virus was detected in 232 of 258 patients by direct sequencing and in 236 of 258 patients by the PCR‐Invader assay. Forty of 231 cases were defined as Y93 mutation by direct sequencing, and 46 of 236 cases were defined as Y93 mutation by the PCR‐Invader assay. SVR of patients who were Y93H by direct sequencing, Y93H by the PCR‐Invader assay, and Y93H by both methods was 62.5%, 82.4%, and 50%, respectively.Conclusions
The sensitivity of the PCR‐Invader assay was similar to that of direct sequencing analysis; however, the PCR‐Invader assay had a better ability to detect minor strains. Combination of the two assays would improve prediction of the response to daclatasvir and asunaprevir, but Y93H mutation had little effect on SVR in ledipasvir and sofosbuvir therapy. 相似文献39.
Noriyuki Akutsu Shigeru Sasaki Takeshi Matsui Hirofumi Akashi Kazuhiko Yonezawa Keisuke Ishigami Masayuki Tsujisaki Hiroyuki Isshiki Atsushi Yawata Satoshi Yamaoka Toshihiro Ban Takeya Adachi Seiya Nakahara Hideyasu Takagi Kohei Nakachi Katsunori Tanaka Takehiro Hirano Itaru Yamamoto Hiroyuki Kaneto Kohei Wagatsuma Yasunao Numata Hiroshi Nakase 《Internal medicine (Tokyo, Japan)》2021,60(21):3369
Objective The change in serum lipid levels by direct-acting antiviral (DAA) treatment for chronic hepatitis C varies depending on the type of DAA. How the lipid level changes induced by glecaprevir-pibrentasvir (G/P) treatment contribute to the clinical outcome remains unclear. We conducted a prospective observational study to evaluate the effectiveness of G/P treatment and the lipid level changes. Methods The primary endpoint was a sustained virologic response at 12 weeks (SVR12). The total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglyceride (TG) levels and LDL-C/HDL-C (L/H) ratio were measured every two weeks. Patients This study included 101 patients. Seventeen cases of liver cirrhosis and nine cases of DAA retreatment were registered. The G/P treatment period was 8 weeks in 74 cases and 12 weeks in 27 cases. Results SVR12 was evaluated in 96 patients. The rate of achievement of SVR12 in the evaluable cases was 100%. We found significantly elevated TC and LDL-C levels over the observation period compared to baseline. The serum levels of HDL-C did not change during treatment but were significantly increased after treatment compared to baseline. The L/H ratio was significantly increased two weeks after the start of treatment but returned to the baseline after treatment. Conclusion The primary endpoint of the SVR12 achievement rate was 100%. G/P treatment changed the serum lipid levels. Specifically, the TC and LDL-C levels increased during and after treatment, and the HDL-C levels increased after treatment. G/P treatment may be associated with a reduced thrombotic risk. Therefore, validation in large trials is recommended. 相似文献
40.
Kunio Kataoka Takuya Ishikawa Eizaburo Ohno Tadashi Iida Hirotaka Suzuki Kota Uetsuki Kazuhiro Furukawa Masanao Nakamura Takashi Honda Masatoshi Ishigami Hiroki Kawashima Yoshiki Hirooka Mitsuhiro Fujishiro 《Pancreatology》2021,21(2):451-458
Background/Objectives: Endoscopic ultrasound elastography (EUS-EG) is useful for diagnosis of small solid pancreatic lesions (SPLs), particularly in excluding pancreatic cancer (PC), but its dependence on main pancreatic duct dilatation (MPDD) has not been examined. We aimed to investigate EUS-EG for diagnosis of small SPLs with and without MPDD.MethodsPatients with pathologically diagnosed SPLs of ≤20 mm were included and retrospectively analyzed. Using the blue:green ratio, an EUS-EG image was classified as blue-dominant, equivalent, or green-dominant. Using multiple EUS-EG images per patient, a lesion with a greater number of blue-dominant than green-dominant images was classified as stiff, and the others as soft. EUS-EG images in random order were judged by three raters. Considering stiff SPLs as PC, diagnostic performance of EUS-EG was examined for SPLs with and without MPDD.ResultsOf 126 cases analyzed, 65 (52%) were diagnosed as PC, and 63 (50%) had MPDD. A total of 1077 EUS-EG images were examined (kappa coefficient = 0.783). Lesions were classified as stiff in 91 cases and soft in 35 (kappa coefficient = 0.932). The ratio of stiff to soft lesions was significantly higher in PC than in non-PC (62:3 vs. 29:32, P < 0.001). The sensitivity, specificity, and negative predictive value of a stiff lesion with vs. without MPDD for diagnosis of PC were 94%, 23%, and 50% vs. 100%, 60%, and 100%, respectively.ConclusionsUsing the EUS-EG stiffness classification for small SPLs, PC can be excluded with high confidence and concordance for a soft lesion without MPDD. 相似文献