雷公藤挥发油对小鼠免疫功能的影响

观察雷公藤挥发油对小鼠免疫功能的影响,发现给药1周后,其可减少脾脏有核细胞数,减少 PFC 和 RFC 数目,抑制脾细胞对 ConA 诱导 T 细胞增殖的反应性,减轻DTH。这说明雷公藤挥发油对小鼠体液免疫和细胞免疫均有明显的抑制作用。     

糖皮质激素及异烟肼预防食管碱性腐蚀伤后瘢痕狭窄的实验研究

实验家犬采用Ｄａｖｉｓ食管碱性腐蚀伤模型，结果显示：甲基强的松龙治疗组，狭窄段食管的管径和食管的顺应性均明显大于对照组，而胶原含量及图象分析瘢痕组织中胶原纤维的密度均显著低于对照组，表明甲基强的松龙确有控制食管腐蚀伤后瘢痕狭窄的作用。但异烟肼组所测得的上述指标，均显著优于甲基强的松龙组，说明异烟肼预防食管腐蚀伤后瘢痕狭窄的效果优于甲基强的松龙，且其不良反应小，可以长期应用。无论甲基强的松龙或异烟肼，如同时应用青霉素均可加强治疗效果。     

Concentric sclerosis (Baló): Morphometric and in situ hybridization study of lesions in six patients

Brain tissues from 6 patients with concentric sclerosis (Baló) were examined by in situ hybridization, immunocytochemistry, morphometry, and histological methods. The patients were 24 to 48 years old and had progressive cerebral symptoms and signs that lasted 15 to 100 days. Large demyelinative lesions, most frequent in the frontal white matter, contained alternating bands of demyelinated and partly myelinated white matter that were arranged in concentric or mosaic patterns. In the areas of demyelination, axons were relatively well preserved and there were perivascular inflammatory infiltrates. In 2 specimens, lesions contained regions with the characteristic appearance of actively demyelinating multiple sclerosis plaques. Oligodendroglial densities were highest in normal-appearing white matter, lower in partially myelinated areas, and lowest in demyelinated areas, which also contained many hypertrophic astrocytes closely associated with oligodendroglia. Messenger RNA levels for myelin-related proteins followed the same pattern; they were lowest in demyelinated areas, higher in partially myelinated areas, and highest in normal-appearing white matter beyond lesion margins. Our findings suggest that concentric sclerosis is a variant of multiple sclerosis, that oligodendroglial loss is important in the pathogenesis of demyelination, and that partially myelinated areas probably represent stages of ongoing myelin breakdown rather than remyelination of previously demyelinated areas.     

硫酸庆大霉素注射液的快速比色分析

本文应用二价铜离子与硫酸庆大霉素在碱性介质中形成有色复合物的原理[1],采用比色法在560nm波长处直接测定硫酸庆大霉素的含量。该法平均回收率为100.2±0.47%。CV=0.47%,测定结果满意。     

成人全唾液与血浆中醛固酮含量测定

本文采用直接放射免疫测定法,固定条件,按标准程序测得30名正常成人全唾液及血浆中的醛固酮(Aldosterone,Aldo)浓度。均值唾液为6.6±1.9nmol/L,血浆为32.5±8.0nmol/L,两者呈平行关系(r=0.4,P<0.05,=11.29+0.79X),浓度比平均为20.2％。结果进一步证实唾液作为临床测定Aldo标本的有效性与实用性,有助于对一些全身及口腔疾病进行诊断及估计预后,并可据以评价皮质类固醇治疗效果及用药的合理性。     

Study of pyogenic granuloma of the oral cavity]

Pyogenic granuloma is one of the diseases sometimes seen in otorhinolaryngology clinics. The clinical features of this disease are understood to be that the lesion is located in the oral cavity in the majority of cases that its causative agent is usually discovered and that it most likely grows as a malignant tumor. However, the entity of pathological diagnosis has not been established. Thirty-one cases of oral pyogenic granuloma, including 16 males and 15 females, are reported in this paper. The granuloma was located most frequently at the tongue, followed, in order, by the gingiva, buccal mucosa, hard palate, lip and oral floor. The period between the patient's first visit to our clinic and the onset of his/her complaint was variable. It was relatively shorter in those cases with the lesion at the gingiva or tongue as compared to other locations. The size of the lesion was smaller than 10 x 10 mm. We classified the pathological features into three patterns; granuloma type, hemangioma type, and intermediate type. Many cases of lesions located at the back of the tongue, buccal mucosa, or hard palate were of the hemangioma type, while many cases of lesions located at the top of the tongue, gingiva, or oral floor were of the granuloma type. We have the impression that pyogenic granuloma could be one of the purulent changes associated with benign oral tumors.     

替代神经吻合法治疗喉返神经麻痹的解剖学研究

通过对30例人喉神经、膈神经、颈袢的解剖观测及利用图象分析仪二维重建技术对相关神经的截面积、纤维数量的测量比较分析,结合有关实验研究资料,设计了在甲状软膏板后下方开窗,于甲杓肌神经起点处切断该神经,用颈袢主支与甲杓肌神经吻合,用膈神经与喉下神经吻合,同时重建声带的内收和外展功能,并探讨了其解剖和生理学基础,认为此手术方法治疗喉返神经麻痹比较理想和可行。     

Protective effect of Re-LPS antiserum on experimental multiple system organ failure.

This study examines the possible beneficial effect of Re-LPS (F515) antiserum on experimental multiple system organ failure (MSOF) in rabbits. The results showed that the plasma LPS level was significantly decreased, and it took a shorter period to clear up LPS in experimental than in control rabbits after receiving Re-LPS antiserum. Pretreatment with antiserum can markedly improve the function of the liver, lungs, kidneys, blood and gastrointestinal tract. The MSOF incidence in the group of rabbits receiving immune sera was only 11.2% and the survival rate was raised by about 40.0%. The results suggest that early passive immunotherapy may neutralize gut-derived endotoxin, inhibit endotoxin-induced mediators release and prevent development of severe complications due to sepsis. It is therefore postulated that LPS core antiserum may provide a prophylactic effect on the development of experimental MSOF.

     

重症胰腺炎动物模型制作及发病机理的研究

为探讨胰腺炎的发病机理，作者采用大鼠（ｎ＝５８只）十二指肠结扎法和犬（ｎ＝４６条）胰管内注射自身胆汁法制作重症胰腺炎的动物模型。该模型接近人类急性胰腺炎的发病因素，方法可靠，操作简便，费用便宜。前者适用于小动物实验，后者宜于大动物实验。作者认为胰管内压力增高和微生物是急性胰腺炎的重要条件；胰酶激活始动时间早在胰管内胆汁和肠液的反流后，胰酶激活的主要部位在腺泡细胞。     

丝烈霉素C诱发小白鼠骨髓细胞姐妹染色单体互换和染色体畸变

丝裂霉素C(MMc)诱导的姐妹染色单体互换(SCE)可反映出细胞DMA损伤修复的基本过程。有关MMC诱发活体的SCE研究不多。本实验目的,就是探讨MMC诱发小白鼠骨髓细胞SCE的剂量—效应关系,并对SCE指标与染色体畸变指标,在检测机体DNA损伤上的差异作一比较研究。结果证明,MMC诱发染色体畸变能办远低于诱发SCE的能力,MMC可诱发小白鼠骨髓细胞SCE产生,SCE值随MMC剂量的增加而增加。SCE即使在低剂量诱变剂的作用下也是敏感的。