Macular buckle technique in myopic traction maculopathy: a 16-year review of the literature and a comparison with vitreous surgery

Graefe's Archive for Clinical and Experimental Ophthalmology - The aim of this study is to review anatomical and functional outcomes following macular buckling (MB) in high myopia and to...     

The role of the medial and central amygdala in stress-induced suppression of pulsatile LH secretion in female rats

Stress exerts profound inhibitory effects on reproductive function by suppressing the pulsatile release of GnRH and therefore LH. Although the mechanisms by which stressors disrupt the hypothalamic GnRH pulse generator remain to be fully elucidated, numerous studies have implicated the amygdala, especially its medial (MeA) and central nuclei (CeA), as key modulators of the neuroendocrine response to stress. In the present study, we investigated the roles of the MeA and CeA in stress-induced suppression of LH pulses. Ovariectomized rats received bilateral ibotenic acid or sham lesions targeting the MeA or CeA; blood samples (25 μl) were taken via chronically implanted cardiac catheters every 5 min for 6 h for the measurement of LH pulses. After 2 h of baseline sampling, the rats were exposed to either: restraint (1 h), insulin-induced hypoglycemia (IIH) (0.3 U/kg, iv), or lipopolysaccharide (LPS) (25 μg/kg, iv) stress. The restraint but not IIH or LPS stress-induced suppression of LH pulses was markedly attenuated by the MeA lesions. In contrast, CeA lesioning attenuated LPS, but not restraint or IIH stress-induced suppression of LH pulses. Moreover, after restraint stress, the number of Fos-positive neurons and the percentage of glutamic acid decarboxylase(67) neurons expressing Fos was significantly greater in the GnRH-rich medial preoptic area (mPOA) of rats with intact, rather than lesioned, MeA. These data indicate that the MeA and CeA play key roles in psychogenic and immunological stress-induced suppression of the GnRH pulse generator, respectively, and the MeA-mediated effect may involve γ-aminobutyric acid ergic signaling within the mPOA.     

PEGylated N-methyl-S-methyl dithiocarbazate as a new reagent for the high-yield preparation of nitrido Tc-99m and Re-188 radiopharmaceuticals

A novel nitrido nitrogen atom donor for the preparation of ^99mTc and ¹⁸⁸Re radiopharmaceuticals containing a metal-nitrogen multiple bond is presented. HO₂C-PEG₆₀₀-DTCZ was obtained by conjugation of N-methyl-S-methyl dithiocarbazate [H₂N–N(CH₃)–C(S)SCH₃, HDTCZ] with polyethylene glycol 600 (PEG₆₀₀). Asymmetrical heterocomplexes of the type [M(N)(PNP)(B)]^0/+ (M=^99mTc, ¹⁸⁸Re; PNP=diphosphine ligands, B=DBODC, DEDC, NSH, H₂OS, CysNAc, HDTCZ) and symmetrical nitride compounds of the type [M(N)(L)₂] (L=DEDC, DPDC) have been prepared in high yield by using the newly designed nitride nitrogen atom donor HO₂C-PEG₆₀₀-DTCZ. A two-step procedure was applied for preparing the above symmetrical and asymmetrical complexes. The first step involved the preliminary formation of a mixture of nitride Tc-99m or Re-188 precursors, which contained the [MN]²⁺ core, through reduction of generator-eluted ^99mTc-pertechnetate or ¹⁸⁸Re-perrhenate with thin (II) chloride in the presence of HO₂C-PEG₆₀₀-DTCZ. In the second step, the intermediate mixture was converted either in the final mixed asymmetrical complex by the simultaneous addition of diphosphine ligand and the suitable bidentate ligand B, or in the final symmetrical complex by the only addition of the bidentate ligand L. It was also demonstrated that the novel water-soluble nitride nitrogen atom donor HO₂C-PEG₆₀₀-DTCZ did not show coordinating properties toward the MN (^99mTc, ¹⁸⁸Re) core. Biodistribution studies in rats of the hitherto unreported [^99mTc(N)(PNP₃)DTCZ]⁺ and [^99mTc(N)(PNP₅)DTCZ]⁺ complexes showed that they selectively localize in the myocardium of rats with a favourable heart-to-lung and heart-to-liver uptake ratios. In particular, the heart-to-lung and heart-to-liver uptake ratios dramatically increased in the interval between 60 and 120 min postinjection. Hence, the combination of the favourable chemical and biological properties of HO₂C-PEG₆₀₀-DTCZ might confer to this novel compound an important role for the development of new ^99mTc and ¹⁸⁸Re-nitrido radiopharmaceuticals.     

Raymond Prince and the R. M. Bucke memorial society for the study of religious experience

The study of the relationship between psychiatry and religion has become an issue of increasing importance for both research and clinical practice. This article presents the history of the R. M. Bucke Memorial Society for the Study of Religious Experience, established by Raymond Prince in Montréal in 1964 as one of the first scientific societies whose aim was to investigate those characteristics of religious experience of interest to psychiatry. It also describes some of Prince's own studies on religious experience.     

Prevalence and clinical significance of antineutrophil cytoplasmic antibodies in Churg‐Strauss syndrome

Objective

Churg‐Strauss syndrome (CSS) is classified among the so‐called antineutrophil cytoplasmic antibody–associated systemic vasculitides (AASVs) because of its clinicopathologic features that overlap with the other AASVs. However, while antineutrophil cytoplasmic antibodies (ANCAs) are consistently found in 75–95% of patients with Wegener's granulomatosis or microscopic polyangiitis, their prevalence in CSS varies widely and their clinical significance remains uncertain. We undertook this study to examine the prevalence and antigen specificity of ANCAs in a large cohort of patients with CSS. Moreover, we evaluated the relationship between ANCA positivity and clinicopathologic features.

Methods

Immunofluorescence and enzyme‐linked immunosorbent assay were used to determine the presence or absence of ANCAs in 93 consecutive patients at the time of diagnosis. The main clinical and pathologic data, obtained by retrospective analysis, were correlated with ANCA status.

Results

ANCAs were present by immunofluorescence in 35 of 93 patients (37.6%). A perinuclear ANCA (pANCA) pattern was found in 26 of 35 patients (74.3%), with specificity for myeloperoxidase (MPO) in 24 patients, while a cytoplasmic ANCA pattern, with specificity for proteinase 3, was found in 3 of 35 patients (8.6%). Atypical patterns were found in 6 of 30 patients with anti‐MPO antibodies (20.0%). ANCA positivity was associated with higher prevalences of renal disease (51.4% versus 12.1%; P < 0.001) and pulmonary hemorrhage (20.0% versus 0.0%; P = 0.001) and, to a lesser extent, with other organ system manifestations (purpura and mononeuritis multiplex), but with lower frequencies of lung disease (34.3% versus 60.3%; P = 0.019) and heart disease (5.7% versus 22.4%; P = 0.042).

Conclusion

ANCAs are present in ∼40% of patients with CSS. A pANCA pattern with specificity for MPO is found in most ANCA‐positive patients. ANCA positivity is mainly associated with glomerular and alveolar capillaritis.