人工流产术后即时口服屈螺酮炔雌醇片避孕效果观察

目的探讨人工流产术后即时服用复方避孕药屈螺酮炔雌醇片的临床效果。方法将160例人工流产患者分为两组,治疗组于人流术后当天服用屈螺酮炔雌醇片、抗生素及益母草颗粒,对照组仅服用抗生素及益母草颗粒,观察术后阴道流血时间、流血量及月经转归时间等情况。结果治疗组术后阴道流血量明显少于对照组(P<0.05);治疗组术后阴道流血时间和月经恢复时间较对照组短,有统计学意义(P<0.05)。结论人工流产术后即时口服避孕药屈螺酮炔雌醇片能减少人流术后并发症,有助于改善妇女生殖健康。     

芦丁稀土配合物的合成及药效学初步研究

目的　探索芦丁与稀土元素配位前后药效学的变化。方法　采用小鼠扭体法和耳壳肿胀法对芦丁与稀土元素配位前后镇痛与抗炎作用进行比较。结果　与NS组比较,芦丁及其配合物可明显减少醋酸所致小鼠扭体次数( P <0 0 5或P <0 0 1);与芦丁组比较,其各配合物组的镇痛作用更强(P <0 0 1)。同样,与NS组比较,芦丁及其配合物对二甲苯引起的小鼠耳壳肿胀有明显的抗炎作用(P <0 0 5或P <0 0 1);而各配合物的抗炎作用又强于芦丁组(P <0 0 5或P <0 0 1)。结论　芦丁稀土配合物较芦丁的镇痛、抗炎作用明显增强。     

A natural lipopeptide of surfactin for oral delivery of insulin

Abstract

Surfactin, a natural lipopeptide produced by Bacillus, is gaining attention for potentially biomedical and pharmaceutical applications. Here, surfactin was assayed for oral delivery of insulin (INS) by its ability to bind to and promote protein to penetrate through the cell membrane. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, surfactin was found to form co-precipitates with INS to protect it from acidic and enzymatic attack in the gastrointestinal tract. Further analysis by non-reductive electrophoresis showed surfactin could bind to INS forming heteropolymers. Analysis with circular dichroism, we found this binding significantly influenced the INS structure with decreased rigid α-helix and β-turn, but with increased flexible β-sheet and random coil. The change with more flexible structure was favorable for INS to penetrate through the cell membrane. Fluorescence spectra analysis also showed surfactin could lead Phe and Tyr in the inner of INS exposed outside, further promoting INS permeabilization by improving the hydrophobic-lipophilic interactions between INS and cell membrane. As a result, the effective permeability (Peff) of INS plus surfactin was 4.3 times of that of INS alone. In vivo assay showed oral INS with surfactin displayed excellent hypoglycemic effects with a relative bioavailability of 12.48% and 5.97% in diabetic mice and non-diabetic dogs, respectively. Summary, surfactin is potential for oral delivery of INS by its role as an effective protease inhibitor and permeability enhancer.     

不同产地人工蛹虫草子实体及冬虫夏草中核苷类成分的比较

目的 测定不同产地人工蛹虫草子实体与天然冬虫夏草中核苷类成分的含量,为人工蛹虫草的质量评价提供依据. 方法 以尿苷、腺苷、虫草素为主要评价指标,采用高效液相色谱法测定9个厂家11批人工蛹虫草样品和4种来源天然冬虫夏草中核苷类成分的含量. 结果 不同产地人工蛹虫草的核苷含量为0.265%～0.870%,冬虫夏草核苷含量为0.168%～0.250%,其中虫草素含量变化较大,而天然冬虫夏草中几乎不含或含有少量虫草素. 结论 产地不同,人工蛹虫草中核苷类成分含量不同,但明显高于天然冬虫夏草中核苷类成分含量,其中腺苷、尿苷含量较为稳定,可为人工蛹虫草的合理开发利用和质量评价提供依据.     

Effects of point application on celiac mast cell degranulation in mice with allergic rhinitis: An experimental study

目的：研究不同处理方法对卵白蛋白（Ovum Albumin,OVA）变应性鼻炎小鼠模型腹腔肥大细胞脱颗粒的影响。方法：将60只雌性小鼠随机分为5组,每组12只,分别按不同方法处理后分离各组小鼠的腹腔肥大细胞,中性红染色后计算腹腔肥大细胞脱颗粒率。结果：正常对照组、OVA变应性鼻炎组、穴位敷贴组、激素对照组、磷酸盐缓冲液（Phosphate Buffer Saline,PBS）阴性对照组小鼠腹腔肥大细胞脱颗粒率分别为（15±6）％、（53±11）％、（37±13）％、（31±15）％、（47±14）％。OVA变应性鼻炎小鼠的腹腔肥大细胞有明显的脱颗粒现象：与OVA变应性鼻炎小鼠相比,穴位敷贴组及激素对照组小鼠的腹腔肥大细胞脱颗粒现象显著减轻;PBS对照组小鼠的腹腔肥大细胞脱颗粒现象没有明显变化。结论：推测穴位敷贴抗过敏机制为稳定肥大细胞膜,抑制肥大细胞脱颗粒,减少致炎介质产生。     

Clinical trial on the role of tuina in rehabilitation therapy following total hip replacement

目的：观察推拿在全髋关节置换术后康复治疗中的临床疗效。方法：将60例患者随机分成治疗组和对照组。治疗组30例进行推拿结合康复治疗,对照组30例进行单纯康复治疗。两组均治疗2星期。分别观察两组患者术后7天、术后2星期、术后6星期的改良人工髋关节Harris量表及汉密尔顿焦虑量表（Hamilton Anxiety Rating Scale,HAMA）。结果：Harris量表评分总分,两组组内患者不同时间点多重比较差异有统计学意义（P〈0．05）;术后1天、术后7天及术后6星期两组间比较,差畀均无统计学意义（A,0．05）;术后2星期两组间比较差异有统计学意义（P〈0．05）。HAMA量表两组组内患者不同时间点多重比较差异有统计学意义（P〈0．05）;术后1天两纽间评分无显著性差异（P〉0．05）;术后7天、术后2星期、术后6星期两组间评分均有显著性差异（P〈0．05）。结论：单位时间内,推拿结合康复治疗对全髋关节置换术后患者的疼痛、关节活动范围及焦虑等方面的改善作用优于单纯康复治疗。     

Treatment of 97 cases of prolapse of lumbar intervertebral disc by manipulations of bone setting plus acupuncture

先应用改良斜扳法和旋转定位扳法对腰椎间盘突出症患者进行手法复位,然后取水沟和委中为主穴,进行针剌治疗.共治疗97例患者,治愈58例,好转35例,无效4例,总有效率95.9%.     

Influence of moxa smoke on mitochondrial transmembrane potential and Bax/Bcl-2 in alveolar type II epithelial A549 cells

Objective

To investigate the influence of moxibustion products on mitochondrial transmembrane potential (MTP) and mRNA expression of Bax/Bcl-2 in alveolar type II epithelial A549 cells, and to further explore influence of moxibustion products on the oxidative damage of A549 cells.

Methods

Smoke and particles generated by moxibustion were collected using the filter box for gas sampling. The moxa smoke extract (MSE) was diluted sequentially to the final concentrations of 0.05 mg/mL, 0.1 mg/mL, 0.2 mg/mL, 0.3 mg/mL and 0.4 mg/mL using the cell culture medium, and A549 cells were then intervened by the above MSE solution. Cell MTP was detected by JC-1 staining. Fluorescence quantitative polymerase chain reaction (PCR) was used to detect Bax/Bcl-2 mRNA expression of A549 cells.

Results

Compared with cells in the normal control group, MTP was significantly decreased in cells of 0.3 mg/mL and 0.4 mg/mL MSE intervention groups (P<0.01); while MTP showed no significant changes in cells of 0.05 mg/mL, 0.1 mg/mL and 0.2 mg/mL MSE intervention groups (P>0.05); compared with cells in 0.05 mg/mL MSE intervention group, MTP was decreased significantly in cells of 0.1 mg/mL, 0.2 mg/mL, 0.3 mg/mL and 0.4 mg/mL MSE intervention groups (P<0.05 ); compared with cells in 0.1 mg/mL MSE intervention group, MTP was decreased significantly in cells of 0.4 mg/mL MSE intervention group (P<0.01). Bax mRNA expression of cells in each concentration of MSE intervention group all showed no significant difference compared to that in the normal control group; Bcl-2 mRNA expression of cells was reduced with the increase of MSE intervention concentration. Wherein, Bcl-2 mRNA expressions of cells in 0.4 mg/mL and 0.3 mg/mL MSE intervention groups were significantly reduced compared with that of cells in the normal control group (P<0.05); Bcl-2 mRNA expression of cells in 0.4 mg/mL MSE intervention group was significantly reduced compared to that in 0.05 mg/mL MSE intervention group (P<0.05).

Conclusion

Certain higher concentration of moxa smoke could reduce MTP and mRNA expression of the anti-apoptosis gene Bcl-2 in alveolar type II epithelial A549 cells. Oxidative damage may be the important mechanism of apoptosis caused by the high concentration of moxa smoke solution, and further studies are necessary on the specific mechanisms.

     

Observation on clinical effect of tuina plus Western medication for functional dyspepsia due to liver qi stagnation and spleen deficiency

Objective

To observe the clinical effects of tuina plus Western medication for functional dyspepsia (FD) due to liver qi stagnation and spleen deficiency.

Methods

total of 72 patients in conformity with the inclusion criteria of FD were randomly divided into an observation group and a control group based upon the random number table, 36 cases in each group. The control group was treated with mosapride citrate dispersible tablets, and the observation group was treated with the same tablets plus tuina. Before the treatment and 4 weeks after the treatment, the clinical symptoms, quality of life (QOL) and depression severity were observed by the scale, and were followed up two months later after the treatment for assessment of the clinical effects.

Results

After the treatment and at the follow-up, the symptom scores of FD and the sores of Hamilton depression rating scale (HAMD) in both groups decreased, and the scores in Chinese version of quality of life questionnaire for functional digestive disorders (Chin-FDDQL) increased, with statistically significant differences in comparison with the same group before the treatment (all P<0.05). In comparison between the two groups at the same time point after the treatment, the scores of FD symptoms, HAMD and Chin-FDDQL were improved better in the observation group than those in the control group, with statistically significant differences (all P<0.05). The total effective rates at the follow-up were 91.7% in the observation group and 75.0% in the control group, without statistical difference between the two groups (P>0.05). The rate of clinical cure and remarkable effect was 66.7% in the observation group, higher than 41.7% in the control group, it is higher in the observation group than that in the control group, with a statistically significant difference between the two groups (P<0.05).

Conclusion

Tuina plus Western medication is precise in the therapeutic effects for FD due to liver qi stagnation and spleen deficiency and can effectively relieve clinical symptoms, elevate the QOL and alleviate depression severity of the patients. Moreover, it’s better than the treatment by Western medication alone in the long-term therapeutic effects.

     

眼科疾病的生物信息学研究进展

生物信息学是一门对基因组学和蛋白质组学进行研究的新兴学科,是生物学、计算机科学、信息工程和统计学的综合交叉学科。在眼科生物基因研究中,角膜、晶状体、房水、玻璃体和视网膜因富含大量的生物信息,是理想的生物信息学研究对象。基因组学检测技术的高效性和准确性有助于对眼科肿瘤及遗传疾病相关差异表达基因的筛查。蛋白质组学有助于分析眼科疾病状态下眼内液体或细胞中基因表达高低所引起的蛋白表达谱及功能改变,从而揭示疾病的发生机制。本文主要综述生物信息学在眼科疾病的应用,并初步展望其对相关疾病治疗的影响、目前存在的问题及未来的发展趋势。