Age-associated changes in the electrophoretic pattern of the high molecular weight non-histone proteins from mouse liver,hepatoma and spleen chromatin

The water-soluble fractions of non-histone chromatin (NHC) proteins were isolated from 0.35M NaCl extracts of liver and spleen chromatin of young and old CBA mice and hepatomas of old mice. SDS-disc electrophoretic patterns of proteins with higher molecular weight were compared. Tissue specificity and age changes of the patterns have been found; however, in spleen chromatin the differences of NHC proteins associated with aging were insignificant. Age-associated changes of NHC proteins in liver (increase of number of minor protein fractions) are not found in chromatin from hepatomas which indicates that the changes of the pattern of NHC proteins in aged liver cells are probably reversible.     

Detection of antibodies to the causative agents of viral infections in multiple sclerosis patients

Serological examinations of blood sera from patients with multiple sclerosis (MS), their nearest relatives, and subjects of the control groups for antibodies to causative agents of some viral infections demonstrated antihemagglutinins to measles and rubella viruses in 61%-95.5% of the subjects examined in all the groups, to mumps virus in 53% in MS patients, to tick-borne encephalitis virus in 2.2% in the same group, and in 10.5% in the group of patients with other CNS diseases, and none in healthy subjects. Virus-neutralizing antibodies to human acute encephalomyelitis virus (HAEM) in 28% of the cases, frequently in the stage of remission. Specific IgM to measles virus was found in 41% of MS patients, in 15% of their nearest relatives, and in 19.7% of patients with other CNS diseases, but not in healthy subjects. No differences in the rate of antibody findings to herpes simplex virus types 1 and 2 were observed in the groups examined. The rate of detection of virus-neutralizing antibody to HAEM virus was significantly higher in MS patients with the severity of the course of IV-V degree (20%) than of the II-III degree (8.8%). In the period of MS exacerbation the level of specific IgM to measles virus increased (35.6%), and higher titres of antihemagglutinins were observed in patients with longer duration of the disease and higher degree of its severity.