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Jaffar M. Khan BM BCh Robert J. Lederman MD 《Catheterization and cardiovascular interventions》2018,91(6):1052-1053
- Transcatheter electrosurgery has emerging value in a range of other new procedures that require traversing tissue (transcaval access, transcatheter Glenn Shunt) or slicing tissue (LAMPOON slicing of the mitral valve and BASILICA slicing of the aortic valve).
- This is the first report of bipolar radiofrequency wires used to cross lesions in humans, reported here in seven re‐entry CTO cases.
- The bipolar configuration may provide directionality to charge without need for wire alignment and advancement, but is theoretically disadvantageous for tissue “cutting” because of problems with charge concentration.
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A study was conducted on the phosphorylation of proteins in the neutrophil cytosol in response to phorbol myristate acetate (PMA) and N- formyl-methionyl-leucyl-phenylalanine (fMLP). Autoradiography of gel electrophoretograms prepared from neutrophils incubated with 32Pi in the presence and absence of the activators showed nine proteins whose state of phosphorylation was affected by neutrophil activation. 32P was gained by eight of these proteins and was lost by the ninth. For all but one of these proteins, the change in the extent of labeling appeared to reach completion by one to two minutes. It was possible to quantitate the changes in 32P content of three of the nine proteins. One of these was the 20-kD protein that lost label when the neutrophils were activated. Quantitation showed that over half the 32P present in this protein in the resting state was gone within 0.2 minutes after activation. The other two were proteins weighing 11 and 69 kD. The phosphorylation characteristics of these two proteins differed, depending on whether activation had been carried out with PMA or fMLP. These differences in protein phosphorylation support other evidence suggesting that PMA and fMLP do not activate neutrophils by identical biochemical pathways. Differences in phosphorylation between resting and activated cells were not affected by dibutyryl cyclic guanosine monophosphate (cGMP), dibutyryl cyclic adenosine monophosphate (cAMP), theophylline, aspirin, hydrocortisone, or colchicine. The differences were abolished, however, by 30 mumol/L trifluoperazine. This finding is consistent with the hypothesis that the calcium/calmodulin system plays a biochemical role in the activation of neutrophils. 相似文献
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强度一时间曲线在腰椎间盘突出症诊断中的价值 总被引:1,自引:0,他引:1
探讨强度-时间曲线对腰椎间盘突出症的诊断价值。测定111例腰椎间盘突出症患者胫前肌、腓骨长肌及拇长伸肌的强度-时间曲线共244条。结果:正常24例,部分变性83例,部分~完全变性4例,阳性率为78.38%;所检测的244条I/T曲线中,正常曲线115条,部分变性125条,完全变性4条,阳性率为52.05%;患者的阳性率与所检查的肌肉数成正比。正常和失神经支配的基强度、时值、利用时比较。基强度的。P〉0.05,时值和利用时的P〈0.05。提示:强度-时间曲线测定对腰椎间盘突出症具有诊断价值。综合多块肌肉的曲线测定可提高阳性率。 相似文献
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